WO2006096648A3 - Modele de souris - Google Patents

Modele de souris Download PDF

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Publication number
WO2006096648A3
WO2006096648A3 PCT/US2006/007941 US2006007941W WO2006096648A3 WO 2006096648 A3 WO2006096648 A3 WO 2006096648A3 US 2006007941 W US2006007941 W US 2006007941W WO 2006096648 A3 WO2006096648 A3 WO 2006096648A3
Authority
WO
WIPO (PCT)
Prior art keywords
mice
relates
present
well
targeting vectors
Prior art date
Application number
PCT/US2006/007941
Other languages
English (en)
Other versions
WO2006096648A2 (fr
Inventor
Nanda A Singh
Mark F Leppert
H Steve White
Karen S Wilcox
Original Assignee
Univ Utah Res Found
Nanda A Singh
Mark F Leppert
H Steve White
Karen S Wilcox
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Utah Res Found, Nanda A Singh, Mark F Leppert, H Steve White, Karen S Wilcox filed Critical Univ Utah Res Found
Publication of WO2006096648A2 publication Critical patent/WO2006096648A2/fr
Publication of WO2006096648A3 publication Critical patent/WO2006096648A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0276—Knock-out vertebrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/035—Animal model for multifactorial diseases
    • A01K2267/0356—Animal model for processes and diseases of the central nervous system, e.g. stress, learning, schizophrenia, pain, epilepsy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Environmental Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Veterinary Medicine (AREA)
  • Biochemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

La présente invention concerne des souris transgéniques, en particulier des souris knock-in (avec insertion d'un gène actif), qui présentent des mutations dans le gène KCNQ2 ou dans le gène KCNQ3, ainsi que le sperme de ces souris. Cette invention concerne également des vecteurs de ciblage conçus pour créer de telles souris. Elle concerne aussi des cellules souches embryonnaires de souris et du sperme cryoconservé comprenant lesdits vecteurs de ciblage, ainsi qu'un procédé de criblage permettant d'identifier des composés utilisés pour traiter des pathologies physiologiques humaines, telles que les convulsions néonatales familiales bénignes (BFNC), les épilepsies partielles, les épilepsies à résistance thérapeutique, les migraines, les douleurs neuropathiques, les accidents vasculaires cérébraux, la démence et l'anxiété.
PCT/US2006/007941 2005-03-07 2006-03-07 Modele de souris WO2006096648A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US65856905P 2005-03-07 2005-03-07
US60/658,569 2005-03-07

Publications (2)

Publication Number Publication Date
WO2006096648A2 WO2006096648A2 (fr) 2006-09-14
WO2006096648A3 true WO2006096648A3 (fr) 2007-07-05

Family

ID=36953935

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/007941 WO2006096648A2 (fr) 2005-03-07 2006-03-07 Modele de souris

Country Status (1)

Country Link
WO (1) WO2006096648A2 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2390348A1 (fr) * 2010-05-25 2011-11-30 Sanofi Procédés et utilisations liées à l'identification d'un composé impliqué dans la douleur et procédés pour le diagnostic de l'algésie
EP2390349A1 (fr) * 2010-05-25 2011-11-30 Sanofi Procédés et utilisations liées à l'identification d'un composé impliqué dans la douleur et procédés pour le diagnostic de l'algésie

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6413719B1 (en) * 1997-10-24 2002-07-02 University Of Utah Research Foundation KCNQ2 and KCNQ3-potassium channel genes which are mutated in benign familial neonatal convulsions (BFNC) and other epilepsies
US6794161B1 (en) * 1999-01-26 2004-09-21 Neurosearch A/S Potassium channels and genes encoding these potassium channels

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6413719B1 (en) * 1997-10-24 2002-07-02 University Of Utah Research Foundation KCNQ2 and KCNQ3-potassium channel genes which are mutated in benign familial neonatal convulsions (BFNC) and other epilepsies
US6794161B1 (en) * 1999-01-26 2004-09-21 Neurosearch A/S Potassium channels and genes encoding these potassium channels

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DEL GIUDICE E.M. ET AL.: "Benign family neonatal convulsions (BFNC) resulting from mutation of the KCNQ2 voltage sensor", EUROPEAN JOURNAL OF HUMAN GENETICS, vol. 8, no. 12, December 2000 (2000-12-01), pages 994 - 997, XP003015158 *
PETERS H.C. ET AL.: "Conditional transgenic suppression of M channels in mouse brain reveals functions in neuronal excitability, resonance and behavior", NATURE NEUROSCIENCE, vol. 8, no. 1, January 2005 (2005-01-01), pages 51 - 60, XP003015160 *
SINGH N.A. ET AL.: "KCNQ2 and KCNQ3 potassium channel genes in benign familial neonatal convulsions: expansion of the functional and mutation spectrum", BRAIN, vol. 126, no. 12, October 2003 (2003-10-01), pages 2726 - 2737, XP003015159 *

Also Published As

Publication number Publication date
WO2006096648A2 (fr) 2006-09-14

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