WO2006080624A1 - Preparation of amino acids/oligopeptides from animal whole blood or clotted blood - Google Patents
Preparation of amino acids/oligopeptides from animal whole blood or clotted blood Download PDFInfo
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- WO2006080624A1 WO2006080624A1 PCT/KR2005/003118 KR2005003118W WO2006080624A1 WO 2006080624 A1 WO2006080624 A1 WO 2006080624A1 KR 2005003118 W KR2005003118 W KR 2005003118W WO 2006080624 A1 WO2006080624 A1 WO 2006080624A1
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- WIPO (PCT)
- Prior art keywords
- amino acids
- oligopeptides
- blood
- clotted blood
- clotted
- Prior art date
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/12—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/06—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from blood
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/341—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins
- A23J3/345—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins of blood proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/24—Animal feeding-stuffs from material of animal origin from blood
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
Definitions
- the present invention relates to a method for preparing natural amino acids/oligopeptides . More particularly, the present invention relates to a method for preparing natural amino acids/oligopeptides with high absorption ratio in the body directly from animal whole blood or clotted blood via protease treatment .
- the present invention provides a more simple and cost-efficient method for preparing amino acids/oligopeptides with higher yield, compared to conventional methods according to the prior art .
- amino acids/oligopeptides may serve as important protein sources, in the case of patients suffering from disorders on the digestive organs such as the stomach, liver and pancreas or subj ected to excision operation thereon, or other convalescent patients , because of their high absorption ratio in the small intestine .
- such disorders cause weakening of immunity, resulting in delay of wound healing at the site subjected to a surgical operation, or complications such as secondary infections . Therefore, it is very important to take various nutritional elements orally, after a surgical operation, for the purpose of better recuperation or convalescence .
- protein is the most fundamental and influential one .
- Amino acids contained in animal foods comprise all of the essential amino acids, and thus have excellent quality from the nutritional point of view.
- incomplete protein foods such as soybeans, are free from at least one essential amino acid. Therefore, use of edible livestock blood, as a starting material for amino acids/oligopeptides, is highly desirable from the nutritional as well as economical points of view .
- the present invention provides a novel method for preparing amino acids/oligopeptides with high purity in a large amount , directly from clotted blood via protease treatment, in a simple, prompt and cost-efficient manner .
- An obj ect of the present invention is to provide a method for preparing amino acids/oligopeptides, which comprises the steps of : clotting animal blood, homogenizing the clotted blood, removing a fat layer from the homogenized clotted blood and degenerating the clotted blood at high temperature; treating the degenerated clotted blood with a protease to perform decomposition of proteins ; filtering the resultant product , treating the filtrate with active carbon and concentrating the filtrate; and drying the concentrate in a short time .
- the present invention provides a novel method for preparing amino acids/oligopeptides directly from animal blood .
- the method according to the present invention comprises the steps of : homogenizing animal clotted blood by a homogenizer and destructing cells so that the clotted blood can react with a protease; heating the homogenized and protease-treated clotted blood to degenerate the clotted blood and treating the resultant clotted blood with a hydrolase to perform hydrolysis ; filtering the protein hydrolyzate obtained from the homogenized clotted blood and concentrating the filtrate; and spray drying the concentrate instantaneously to obtain brown powder containing amino acids/oligopeptides .
- the method according to a preferred embodiment of the present invention comprises the steps of : comminuting and homogenizing clotted animal blood so as to facilitate enzymatic hydrolysis of the clotted blood; removing a fat layer from the homogenized clotted blood; treating the homogenized clotted blood with a protease to perform hydrolysis efficiently, and thus to facilitate preparation of amino acids/oligopeptides ; separating amino acids/oligopeptides from the protein hydrolyzate via ultrafiltration; further filtering the filtrate with active carbon to remove odors and impurities ; concentrating the filtrate by a reverse osmosis .system; and drying the concentrate to provide brown powder containing amino acids/oligopeptides .
- animal includes all livestock or animals except those excluded due to aesthetic and hygienic reasons .
- animal comprises cows , pigs, horses, goats , sheep, dogs , birds, whales, or the like .
- water is added to clotted blood, from which sera are removed or not, in an amount of 1-5 times based on the amount of clotted blood, and then the resultant mixture is homogenized and subj ected to cell destruction by • being homogenized sufficiently with a homogenizer at 1, 000-10 , 000 rpm.
- the above-described step allows animal clotted blood to be in better contact with a protease , so that yield of amino acids/oligopeptides can be increased. Then, the homogenized animal clotted blood obtained from the above step is passed to a separation system, in which the uppermost fat layer and insoluble materials are separated from the homogenized clotted blood.
- the homogenizing and cell- destructing step results in an improvement in the hydrolysis degree of clotted blood .
- the first step facilitates removal of polypeptides or non-hydrolyzed protein components during the following step of separating amino acids/oligopeptides from the hydrolyzate of clotted blood.
- the homogenized clotted blood is transferred to a water bath thermostat, pH of the clotted blood is adjusted to pH 10-11 and warmed the clotted blood to 90 ° C to perform degeneration of proteins for 1 hour .
- the degenerated clotted blood are treated with a protease under the conditions of a pH of 8.0-10.5 and a temperature of 50-60 ° C
- the hydrolysis reaction is performed for 2-24 hours .
- the alkalase may be any one of the commercially available alkalases .
- the hydrolyzate in the reaction mixture is subj ected to ultrafiltration, so as to separate amino acids/oligopeptides efficiently from the reaction mixture .
- the filtrate obtained from the ultrafiltration step is further filtered with active carbon, so as to remove unique odors and impurities from the filtrate, and thus to improve the quality of amino acids/oligopeptides and to provide edible amino acids/oligopeptides .
- the filtrate containing amino acids/oligopeptides is concentrated by a reverse osmosis system, so as to reduce the water content .
- the concentrate of amino acids/oligopeptides obtained from the reverse osmosis step is dried at 196-202 ° C for 10-40 seconds to provide amino acid/oligopeptide fractions .
- drying of the amino acid/oligopeptide concentrate may be performed by any one drying process of hot air drying, spray drying, vacuum freeze-drying, vacuum heat drying, depressurized heat drying, or the like .
- the finally dried product is light brown powder containing 20.0-40.0% (w/w) of amino acids and 40.0-50.0 % (w/w) of amino acids/oligopeptides .
- FIG. 1 is a mass spectrum of amino acids/oligopeptides in the product obtained by the method according to a preferred embodiment of the present invention .
- the homogenized product was transferred to a separation flask to collect the bottom layer .
- the bottom layer was transferred to a water bath thermostat, adjusted to pH 10- 11, warmed to 90 "Q and then subj ected to degeneration of proteins for 1 hour .
- 1-3 mL/kg of alkalase was added thereto .
- the pH of the reaction mixture was maintained at 8-10.5 by using 4-6 N NaOH, and then the reaction mixture was allowed to react for 2-24 hours while maintaining the reaction temperature at 50-60 ° C
- the hydrolyzate in the reaction mixture was subj ected to ultrafiltration (polyacrylonitrile membrane, Chemicore, Daej eon, Republic of Korea) to separate out amino acids/oligopeptides .
- Example 1 Each product (sample 1, sample 2 and sample 3 ) obtained from the above Example 1 was analyzed for nutritional elements .
- Each sample comprised proteins and carbohydrates in a ratio of 1 : 1 , and is substantially free from fats .
- Amino acid contents of each amino acid/oligopeptide product are shown in the following Table 1. Particularly, Table 2 shows the amino acid composition of sample 1. Additionally, the mass spectrum for amino acids/oligopeptides contained in the product ( sample 1) is shown in FIG. 1. [Table 1]
- GLX represents glutamine and glutamic acid in total .
- the method of the present invention it is possible to obtain high-purity natural amino acids/oligopeptides by treating animal whole blood or clotted blood directly with an enzyme to perform decomposition of proteins, filtering and concentrating the resultant product, and drying the resultant concentrate .
- the amino acids/oligopeptides obtained from the above method show a high absorption ratio in the small intestine, and thus can be used as a meal substitute for patients and are useful for ' food additives . Therefore, the present invention is a very useful invention in the medical and food industrial fields .
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Abstract
The present invention relates to a method for preparing amino acids/oligopeptides, which comprises treating animal whole blood or clotted blood directly with a protease and separating amino acids/oligopeptides from the reaction mixture. The method is more simple and cost-efficient and provides higher yield, compared to conventional methods, and thus is very useful in the medical and food industrial fields.
Description
DESCRIPTION
PREPARATION OF AMINO ACIDS/OLIGOPEPTIDES FROM ANIMAL WHOLE BLOOD OR CLOTTED BLOOD
TECHNICAL FIELD
The present invention relates to a method for preparing natural amino acids/oligopeptides . More particularly, the present invention relates to a method for preparing natural amino acids/oligopeptides with high absorption ratio in the body directly from animal whole blood or clotted blood via protease treatment . The present invention provides a more simple and cost-efficient method for preparing amino acids/oligopeptides with higher yield, compared to conventional methods according to the prior art .
BACKGROUND ART
In general, amino acids/oligopeptides may serve as important protein sources, in the case of patients suffering from disorders on the digestive organs such as the stomach, liver and pancreas or subj ected to excision operation thereon, or other convalescent patients , because of their high absorption ratio in the small intestine . A disorder in oral absorption of proteins, occurring after a surgical excision operation, results in a protein deficiency from the nutritional point of view. Also, such
disorders cause weakening of immunity, resulting in delay of wound healing at the site subjected to a surgical operation, or complications such as secondary infections . Therefore, it is very important to take various nutritional elements orally, after a surgical operation, for the purpose of better recuperation or convalescence . Among such nutritional elements , protein is the most fundamental and influential one .
Amino acids contained in animal foods comprise all of the essential amino acids, and thus have excellent quality from the nutritional point of view. On the contrary, incomplete protein foods, such as soybeans, are free from at least one essential amino acid. Therefore, use of edible livestock blood, as a starting material for amino acids/oligopeptides, is highly desirable from the nutritional as well as economical points of view .
Although blood discharged upon butchery of cows or pigs is an important resource for amino acids/oligopeptides , there are no commercially available methods for preparing amino acids/oligopeptides directly from blood. Herein, the animal blood, from which amino acids/oligopeptides may be prepared, can be obtained in the form of clotted blood in butcheries .
Now, the present invention provides a novel method for preparing amino acids/oligopeptides with high purity in a
large amount , directly from clotted blood via protease treatment, in a simple, prompt and cost-efficient manner .
Disclosure of the Invention TECHNICAL PROBLEMS
An obj ect of the present invention is to provide a method for preparing amino acids/oligopeptides, which comprises the steps of : clotting animal blood, homogenizing the clotted blood, removing a fat layer from the homogenized clotted blood and degenerating the clotted blood at high temperature; treating the degenerated clotted blood with a protease to perform decomposition of proteins ; filtering the resultant product , treating the filtrate with active carbon and concentrating the filtrate; and drying the concentrate in a short time .
TECHNICAL SOLUTIONS
The present invention provides a novel method for preparing amino acids/oligopeptides directly from animal blood .
The method according to the present invention comprises the steps of : homogenizing animal clotted blood by a homogenizer and destructing cells so that the clotted blood can react with a protease; heating the homogenized and protease-treated clotted blood to degenerate the
clotted blood and treating the resultant clotted blood with a hydrolase to perform hydrolysis ; filtering the protein hydrolyzate obtained from the homogenized clotted blood and concentrating the filtrate; and spray drying the concentrate instantaneously to obtain brown powder containing amino acids/oligopeptides .
More particularly, the method according to a preferred embodiment of the present invention comprises the steps of : comminuting and homogenizing clotted animal blood so as to facilitate enzymatic hydrolysis of the clotted blood; removing a fat layer from the homogenized clotted blood; treating the homogenized clotted blood with a protease to perform hydrolysis efficiently, and thus to facilitate preparation of amino acids/oligopeptides ; separating amino acids/oligopeptides from the protein hydrolyzate via ultrafiltration; further filtering the filtrate with active carbon to remove odors and impurities ; concentrating the filtrate by a reverse osmosis .system; and drying the concentrate to provide brown powder containing amino acids/oligopeptides .
As used herein, the term "animal" includes all livestock or animals except those excluded due to aesthetic and hygienic reasons . For example, "animal" comprises cows , pigs, horses, goats , sheep, dogs , birds, whales, or the like .
In the method according to a preferred embodiment of the present invention, water is added to clotted blood, from which sera are removed or not, in an amount of 1-5 times based on the amount of clotted blood, and then the resultant mixture is homogenized and subj ected to cell destruction by • being homogenized sufficiently with a homogenizer at 1, 000-10 , 000 rpm. The above-described step allows animal clotted blood to be in better contact with a protease , so that yield of amino acids/oligopeptides can be increased. Then, the homogenized animal clotted blood obtained from the above step is passed to a separation system, in which the uppermost fat layer and insoluble materials are separated from the homogenized clotted blood.
As described above, the homogenizing and cell- destructing step results in an improvement in the hydrolysis degree of clotted blood . Additionally, the first step facilitates removal of polypeptides or non-hydrolyzed protein components during the following step of separating amino acids/oligopeptides from the hydrolyzate of clotted blood.
Then, according to a preferred embodiment of the present invention, in order to perform hydrolysis efficiently, and thus to facilitate preparation of amino acids/oligopeptides, the homogenized clotted blood is transferred to a water bath thermostat, pH of the clotted
blood is adjusted to pH 10-11 and warmed the clotted blood to 90 °C to perform degeneration of proteins for 1 hour . Next, the degenerated clotted blood are treated with a protease under the conditions of a pH of 8.0-10.5 and a temperature of 50-60 °C
As the protease, 1-3 mL/kg of alkalase is added. The hydrolysis reaction is performed for 2-24 hours . The alkalase may be any one of the commercially available alkalases . Then, the hydrolyzate in the reaction mixture is subj ected to ultrafiltration, so as to separate amino acids/oligopeptides efficiently from the reaction mixture .
Then, the filtrate obtained from the ultrafiltration step is further filtered with active carbon, so as to remove unique odors and impurities from the filtrate, and thus to improve the quality of amino acids/oligopeptides and to provide edible amino acids/oligopeptides .
After the ultrafiltration with active carbon, the filtrate containing amino acids/oligopeptides is concentrated by a reverse osmosis system, so as to reduce the water content .
Finally, the concentrate of amino acids/oligopeptides obtained from the reverse osmosis step is dried at 196-202 °C for 10-40 seconds to provide amino acid/oligopeptide fractions . More particularly, drying of the amino
acid/oligopeptide concentrate may be performed by any one drying process of hot air drying, spray drying, vacuum freeze-drying, vacuum heat drying, depressurized heat drying, or the like . The finally dried product is light brown powder containing 20.0-40.0% (w/w) of amino acids and 40.0-50.0 % (w/w) of amino acids/oligopeptides .
BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 is a mass spectrum of amino acids/oligopeptides in the product obtained by the method according to a preferred embodiment of the present invention .
BEST MODE
The present invention will be described in detail by preferable embodiments as the following description. It is to be understood that the following examples are illustrative only and the scope of the present invention is not limited thereto .
Example 1 : Preparation of Amino Acids/Oligopeptides according to the present invention
About 200 g of sera were removed from 1.2 kg of clotted blood of pigs by sieving . Then, 1 kg of water was added to the remaining clotted blood, and then the resultant mixture was introduced into a homogenizer and
homogenized at 3, 000 rpm / room temperature for 30 minutes .
The homogenized product was transferred to a separation flask to collect the bottom layer . The bottom layer was transferred to a water bath thermostat, adjusted to pH 10- 11, warmed to 90 "Q and then subj ected to degeneration of proteins for 1 hour . Then, 1-3 mL/kg of alkalase was added thereto . Herein, the pH of the reaction mixture was maintained at 8-10.5 by using 4-6 N NaOH, and then the reaction mixture was allowed to react for 2-24 hours while maintaining the reaction temperature at 50-60 °C The hydrolyzate in the reaction mixture was subj ected to ultrafiltration (polyacrylonitrile membrane, Chemicore, Daej eon, Republic of Korea) to separate out amino acids/oligopeptides . Then, the filtrate was subj ected to filtration with active carbon, so as to remove odors and impurities . The filtrate was concentrated by removing water therefrom in a reverse osmosis system. Finally, the concentrate was spray-dried at 196-202 °C to provide sample 1. Samples 2 and 3 were obtained in the same manner as described above .
Example 2 : Analysis for Nutritional Elements
Each product (sample 1, sample 2 and sample 3 ) obtained from the above Example 1 was analyzed for nutritional elements . Each sample comprised proteins and
carbohydrates in a ratio of 1 : 1 , and is substantially free from fats . Amino acid contents of each amino acid/oligopeptide product are shown in the following Table 1. Particularly, Table 2 shows the amino acid composition of sample 1. Additionally, the mass spectrum for amino acids/oligopeptides contained in the product ( sample 1) is shown in FIG. 1. [Table 1]
Amino Acid content
Amino acid content in Amino acid content total protein (%) D in total sample (% )
Sample 1 40 2 20.1
Sample 2 60 2 30.1
Sample 3 83 .4 41.7 l) Total protein content is measured by the Mini- Kj eldahl method.
[Table 2]
Amino Acid Composition
* CYA represents cystein and cystine in total .
** ASX represents asparagine and aspartic acid in total .
*** GLX represents glutamine and glutamic acid in total .
INDUSTRIAL APPLICABILITY
As described above, according to the method of the present invention, it is possible to obtain high-purity natural amino acids/oligopeptides by treating animal whole blood or clotted blood directly with an enzyme to perform decomposition of proteins, filtering and concentrating the resultant product, and drying the resultant concentrate . The amino acids/oligopeptides obtained from the above method show a high absorption ratio in the small intestine, and thus can be used as a meal substitute for patients and
are useful for' food additives . Therefore, the present invention is a very useful invention in the medical and food industrial fields .
Claims
1. A method for preparing amino acids/oligopeptides from animal whole blood or clotted blood, which comprises the steps of : comminuting and homogenizing the animal whole blood or clotted blood; removing a fat layer and insoluble materials from the homogenized clotted blood; treating the homogenized clotted blood with a protease to perform hydrolysis of proteins ; separating amino acids/oligopeptides from the protein hydrolyzate of the clotted blood via filtration; concentrating the filtrate containing amino acids/oligopeptides ; and drying the concentrate containing amino acids/oligopeptides .
2. The method for preparing amino acids/oligopeptides according to claim 1, wherein the homogenization of animal whole blood or clotted blood is performed by adding water to the animal whole blood or clotted blood, from which sera are removed or not, in an amount of 1-5 times based on the amount of the animal whole blood or clotted blood, and then homogenizing the resultant mixture by using a homogenizer at 1, 000-10 , 000 rpm.
3. The method for preparing amino acids/oligopeptides according to claim 1, wherein the hydrolysis of proteins of the homogenized clotted blood is performed by treating the homogenized clotted blood with a protease under the conditions of a pH of 8.0-10.5 and a temperature of 50-60 °C
4. The method for preparing amino acids/oligopeptides according to claim 3 , wherein the treatment with a protease is performed, after the steps of : transferring the homogenized clotted blood to a water bath thermostat, adj usting the pH to 10-11, and warming the homogenized clotted blood to a temperature of 90 °C to perform degeneration of the proteins for 1 hour .
5. The method for preparing amino acids/oligopeptides according to claim 3 , wherein the hydrolysis of proteins is performed for 2-24 hours .
6. The method for preparing amino acids/oligopeptides according to claim 1, wherein the filtering step of the protein hydrolyzate of the clotted blood is performed by ultrafiltration .
7. The method for preparing amino acids/oligopeptides according to claim 6, wherein the filtrate obtained from the ultrafiltration is further filtered with active carbon in order to remove odors and impurities .
8. The method for preparing amino acids/oligopeptides according to claim 1, wherein the concentration of the filtrate containing amino acids/oligopeptides is carried out in a reverse osmosis system.
9. The method for preparing amino acids/oligopeptides according to claim 1 , wherein the drying step of the concentrate containing amino acids/oligopeptides is carried out at 19β-202 °C within a time of 10-40 seconds , by using any one selected from the group consisting of hot air drying, spray drying, vacuum freeze-drying, vacuum heat drying and depressurized heat drying .
10. The method for preparing amino acids/oligopeptides according to claim 1, wherein the animal comprises cows, pigs, horses , goats , sheep, dogs , birds and whales .
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020040075329A KR100694662B1 (en) | 2004-09-21 | 2004-09-21 | Preparation of amino acids/oligopeptides from animal whole blood or clotted blood |
| KR10-2004-0075329 | 2004-09-21 |
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| Publication Number | Publication Date |
|---|---|
| WO2006080624A1 true WO2006080624A1 (en) | 2006-08-03 |
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|---|---|---|---|
| PCT/KR2005/003118 WO2006080624A1 (en) | 2004-09-21 | 2005-09-21 | Preparation of amino acids/oligopeptides from animal whole blood or clotted blood |
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| WO (1) | WO2006080624A1 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101613734B (en) * | 2009-08-17 | 2011-09-07 | 张吉越 | Method for preparing plasma polypeptide |
| EP3643181A1 (en) * | 2018-10-26 | 2020-04-29 | Dagon Products BVBA | Food supplement and method for the manufacturing thereof |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106805251B (en) * | 2017-01-18 | 2020-06-16 | 福建农林大学 | Polypeptide-curdlan composite gel and preparation method thereof |
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| JPS63283553A (en) * | 1987-05-15 | 1988-11-21 | Niigata Eng Co Ltd | Production of sterilized blood powder |
| JPS63287449A (en) * | 1987-05-20 | 1988-11-24 | Gunei Kagaku Kogyo Kk | Pet food added by enzyme hydrolysate of blood-corpuscle |
| JPS63294733A (en) * | 1987-05-28 | 1988-12-01 | 三菱重工業株式会社 | Concentration of blood |
| JPS63301773A (en) * | 1987-05-30 | 1988-12-08 | Shinko Dousan Kk | Treatment of animal blood |
| KR910009177A (en) * | 1989-11-22 | 1991-06-28 | 권태완 | Manufacturing method of powdered seasoning food using livestock's blood |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20010038713A (en) * | 1999-10-27 | 2001-05-15 | 최상원 | Method for preparing amino acids and/or oligopeptides |
| KR100406171B1 (en) * | 2000-11-24 | 2003-11-17 | (주)바이오넬 | Preparation methods of amino acids from dry blood powder |
| KR100420824B1 (en) * | 2001-06-21 | 2004-03-02 | 배도규 | Silk peptide and silk fibroin powder materials made by silk and manufacturing method for those materials |
-
2004
- 2004-09-21 KR KR1020040075329A patent/KR100694662B1/en active IP Right Grant
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2005
- 2005-09-21 WO PCT/KR2005/003118 patent/WO2006080624A1/en active Application Filing
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63283553A (en) * | 1987-05-15 | 1988-11-21 | Niigata Eng Co Ltd | Production of sterilized blood powder |
| JPS63287449A (en) * | 1987-05-20 | 1988-11-24 | Gunei Kagaku Kogyo Kk | Pet food added by enzyme hydrolysate of blood-corpuscle |
| JPS63294733A (en) * | 1987-05-28 | 1988-12-01 | 三菱重工業株式会社 | Concentration of blood |
| JPS63301773A (en) * | 1987-05-30 | 1988-12-08 | Shinko Dousan Kk | Treatment of animal blood |
| KR910009177A (en) * | 1989-11-22 | 1991-06-28 | 권태완 | Manufacturing method of powdered seasoning food using livestock's blood |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101613734B (en) * | 2009-08-17 | 2011-09-07 | 张吉越 | Method for preparing plasma polypeptide |
| EP3643181A1 (en) * | 2018-10-26 | 2020-04-29 | Dagon Products BVBA | Food supplement and method for the manufacturing thereof |
| BE1026731B1 (en) * | 2018-10-26 | 2020-06-03 | Dagon Products Bvba | Dietary supplement and method for its manufacture |
Also Published As
| Publication number | Publication date |
|---|---|
| KR100694662B1 (en) | 2007-03-13 |
| KR20060026548A (en) | 2006-03-24 |
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