KR20010038713A - Method for preparing amino acids and/or oligopeptides - Google Patents

Abstract

PURPOSE: A method for producing amino acids and/or oligo peptides is provided, thereby amino acids and oligo peptides having no side effects can be produced with low costs. CONSTITUTION: The method for producing amino acids and/or oligo peptides comprises the steps of: hydrolyzing waste products or by-products containing 3 wt.% or more proteins by adding an alkali aqueous solution thereinto and reacting at 80 to 170 deg. C for 10 minutes to 2 hours to produce amino acids and/or oligo peptides metal salts; and removing metals by adding acid into the proteins and/or oligo peptides metal salts, in which the waste products or byproducts are obtained from the leather industry, fish industry or stock farmer; the alkali aqueous solution is selected from the group consisting of sodium hydroxide solution, kalium hydroxide solution and calcium hydroxide solution; and the acid is selected from the group consisting of sulfuric acid solution, oxalic acid solution, carbonate gas and hypochlorite.

Description

Method for preparing amino acids and / or oligopeptides {METHOD FOR PREPARING AMINO ACIDS AND / OR OLIGOPEPTIDES}

The present invention relates to a method for preparing amino acids and / or oligopeptides, and in particular, a method for producing amino acids and / or oligopeptides by alkali hydrolysis of neutralized livestock waste and leather waste, followed by neutralization to remove metal salts. It is about.

All animals need to consume protein, which is the body's constituent and energy source, in order to be active. Proteins are the most important biological components in vivo and are polymers of polypeptide chains in which about 20 amino acids are linked by peptide bonds. Numerous proteins with different molecular weights exist according to the number, type, and sequence of amino acids. The composition is not constant, but the average protein is 53% carbon, 23% oxygen, 16% nitrogen, 7% hydrogen, and 1% sulfur. Have When the protein is directly taken as food, a large amount of the protein is not absorbed and released into the body due to the difference in digestibility and absorption rate depending on the properties of the protein. Special proteins may contain phosphorus, iron, iodine, copper, manganese and zinc.

The role of minerals in vivo is known. Firstly, sodium, magnesium, phosphorus, and chlorine dissolve in body fluids, stimulating muscles and nerve tissues to make the muscles elastic, which can also excite or calm it. In addition, by controlling the pH and osmotic pressure plays a large role in maintaining the normal physicochemical action in vivo. The pH of the blood is maintained at 7.3-7.5 because the mineral buffers. Second, as a component, calcium and phosphorus are components of hard tissues such as bones and teeth, and phosphorus binds to organic compounds to form nuclear proteins and phospholipids, and is involved in the metabolism of organisms as ATP components. Third is the catalytic function. Magnesium, manganese, copper, zinc and iron are involved in the activation of enzymatic reactions and iron, zinc, sulfur, phosphorus, selenium, molybdenum and the like are components of the enzyme. However, when these minerals are present in excess, their side effects occur.

Amino acids absorbed by digested protein from food are mixed with endogenous amino acids resulting from the breakdown of body tissues, forming a so-called amino acid pool. These amino acids are used for the synthesis of body proteins, hormones, enzymes, etc. The remaining ones are used for energy by undergoing oxidative degradation.

At this time, the α-amino group of the L-amino acid is removed by oxidative degradation. This amino group is not reused in the synthesis of new or other nitrogen-containing products, and in vertebrates it is formed and excreted as urea.

Such amino acids are industrially obtained from hydrolysis of proteins. Hydrolysis of proteins is a chemical reaction that hydrolyzes peptide bonds of proteins to produce amino acids or peptides, also called proteolysis. Decomposition methods include thermal hydrolysis, acid-hydrolysis, alkali-hydrolysis, and hydrolysis using enzymes. A typical process for producing amino acids and oligopeptides is a thermal hydrolysis process, in which the protein-containing material as a raw material is hydrolyzed as pressurized steam and crushed after direct drying. This method is relatively simpler than other methods and is the most widely used, but has a very low degradation rate, so it shows only about 50% absorption when used as a feed product.

In the hydrolysis by acid, it is required to perform the hydrolysis process at about 110 ° C. for at least 24 hours, and there is a disadvantage that tryptophan is destroyed during the process. The hydrolysis method using enzymes has a problem that it takes a long time and the yield is low, and the production cost of amino acids is expensive.

It is essential to remove metal salts from amino acids and oligopeptides used as feed because the animal may ingest excess salt when used as feed for the animal.

The general salt removal method is known to use a variety of solubility difference for each salt with temperature. When sodium carbonate (Na ₂ CO ₃ ), sodium chloride (NaCl), and sodium sulfate (Na ₂ SO ₄ ) are mixed, the solution is first treated with sulfuric acid to convert the carbonate to the sulfate form, which is then heated to 120-130 ° C. in the process of recrystallization. To convert sodium chloride to sulphate to regenerate (US Pat. No. 3,833,462), and if sodium sulphate is mixed in the aqueous slurry, sodium sulphate is poured by cold water to recrystallize (US Pat. No. 4,246,241), soluble citric acid, In order to remove sodium sulfate from dific acid and phosphate buffer solution, there is a method of sufficiently absorbing sulfur dioxide (SO ₂ ) gas and heating and precipitating separation (US Pat. No. 5,108,625).

Korean Patent Application No. 99-4886 discloses a technique for producing excess amino acid salts and oligopeptide salts by alkali hydrolysis of wastes containing proteins.

The object of the present invention is to improve the removal method of metal salts from amino acids and oligopeptides with very complicated removal process and low yield, so that the process is simple and the metal salt removal rate is close to 100%. It is an object of the present invention to provide a method for preparing an amino acid and / or oligopeptide.

Another object of the present invention is to provide a method of using an amino acid and an oligo peptide prepared according to the above method.

In order to achieve the above object, in the present invention

Preparing an amino acid and / or oligopeptide metal salt by performing an alkali hydrolysis reaction by adding an aqueous alkali solution to a waste or by-product having a protein content of 3% or more by weight; And

It provides a method for producing an amino acid and / or oligo peptide comprising the step of adding an acid to the metal salt to remove the metal component.

As the wastes or by-products, wastes, by-products and the like obtained from the activities of the leather industry, fishery or livestock industry are preferably used. There is no particular limitation on the amount of alkali component added for alkali hydrolysis of such wastes or by-products, but repeated experiments show that it is appropriate to use 40-90 parts by weight of an aqueous alkaline solution based on about 5-50 parts by weight of wastes or by-products. I could confirm it.

In the present invention, amino acids and / or amino acids that can be used as animal feed or feed additives by removing metal components from amino acids or oligopeptide metal salts produced by alkali hydrolysis of proteins in leather processing, fishery and livestock waste or by-products as raw materials The preparation and application of oligopeptides is presented.

The present invention also provides an inexpensive amino acid salt and oligopeptide salt obtained by alkali hydrolysis of a waste containing protein as feed or feed additive, thereby removing excess metal components present in them, thereby inexpensive amino acids and And / or techniques for preparing oligopeptides.

Hereinafter, the present invention will be described in more detail.

Referring to the salt removal process of the present invention in detail as follows. First, when alkali hydrolysis of waste or by-products containing proteins, the resulting amino acid salt, oligopeptide salt and excess alkali are present in the reaction vessel. The alkali hydrolysis reaction formula is as follows.

NH ₂ -CHR-CO- (NH-CHR-CO) _n-2 -NH-CHR-COOH + nMOH → nNH ₂ -CHR-COOM-(1)

NH ₂ -CHR-CO- (NH-CHR-CO) _n-2 NH-CHR-COOH + n / m + 2 MOH → n / m + 2 NH ₂ -CHR-CO- (NH-CHR-CO) _m -NH-CHR-COOM --- (2)

In the formula, M means metal ions such as sodium, calcium, potassium ions, R is an alkyl group, n and m means an integer. Formula (1) is a hydrolysis reaction to generate an amino acid salt, and formula (2) is a hydrolysis reaction to generate an oligo peptide salt.

Under normal pressure or pressurized conditions, the reaction temperature is adjusted to about 80-170 ° C., and the reaction is allowed to proceed with stirring using a stirrer. The reaction time may vary depending on the pressure conditions, the amount of the reactants, the amount or the concentration of the alkali component, but about 10 minutes to 2 hours after the temperature is sufficient. If the pressure is high during the reaction, the reaction is terminated within a short time even at low temperatures, and if the pressure is low, the reaction temperature is increased or the reaction takes a long time, so these conditions are appropriately selected according to the situation. Do not. The method of removing the salt and excess alkali thus produced is to neutralize it with acid to effectively remove the salt.

When acid is added to the amino acid salt, a neutralization reaction is carried out through the isoelectric point, and the new anion salt becomes an amine salt form of the amino acid in the acid region through the isoelectric point to remove the metal.

nNH ₂ -CHR-COOM + aMOH + aHA → nNH ₂ -CHR-COOM + aMA --- (3)

_{nNH 2 -CHR-COOM + nHA →} nNH 3 + -CHR-COO - + MA --- (4)

_{^{nNH 3 + -CHR-COO - +}} nHA → nA - NH 3 + -CHR-COOH --- (5)

In the formulas (3) to (5), M means a metal ion, HA means an acid, n and a means an integer. Equation (3) is a reaction scheme in which an excess of alkali is removed by titrating an acid to a hydrolyzed amino acid salt and an excess of alkali. Equation (4) is a reaction scheme in which an amino acid is formed through an isoelectric point when an acid is added to an amino acid salt. Equation (5) is a reaction in which a new anionic salt is formed by the amphoteric amphoteric and amino acid zwitterionicity. The isoelectric point represents the state where the charge of the amphoteric electrolyte in the aqueous solution becomes logarithmic to zero as the hydrogen index pH of the solution.

Livestock wastes and leather wastes are used as raw materials containing a large amount of protein as a raw material for generating amino acid salts. The composition ratios and properties of some protein-containing wastes are shown in Table 1 below.

Ingredient Ratio and Properties of Raw Materials division protein(%) Fat(%) moisture(%) Constellation Livestock waste (chicken slaughter) feather 72 11 13 A damp state Etc 65 13 15 A damp state Leather waste fur 26 13 55 Half price leather 22 13 60 Half price

The protein content of the waste and by-products of livestock, leather and aquatic products that can be used as raw materials is more than 3% by weight, and the higher the protein content, the simpler the removal of soap components by fat.

An alkaline process for the production of amino acid salts was carried out using waste containing an excess of such protein as a raw material as follows.

<Experimental example 1>

40 parts by weight of cowhide waste and 60 parts by weight of 10% aqueous sodium hydroxide solution were placed in a reaction vessel and heated to 160 ° C while stirring under atmospheric pressure. After stirring for 2 hours, the reaction was stopped, cooled to room temperature, and the mesh was filtered through a fine wire mesh to remove foreign substances such as unreacted trees, bones, and chips, and discarded to obtain a mixture containing amino acid sodium salt and / or oligopeptide salt. It was.

<Experiment 2>

20 parts by weight of chicken processing waste and 80 parts by weight of 10% aqueous sodium hydroxide solution were placed in a reaction vessel and heated to 160 ° C. while stirring under atmospheric pressure. Hereinafter, a process was performed according to the same method as Experimental Example 1 to obtain a mixture including the amino acid sodium salt and / or oligo peptide salt.

<Experiment 3>

40 parts by weight of cowhide waste and 80 parts by weight of 10% aqueous potassium hydroxide solution were placed in a reaction vessel and heated to 160 ° C while stirring under atmospheric pressure. Hereinafter, a process was performed according to the same method as Experimental Example 1 to obtain a mixture including the amino acid sodium salt and / or oligo peptide salt.

<Experiment 4>

20 parts by weight of chicken processing waste and 80 parts by weight of 10% aqueous potassium hydroxide solution were placed in a reaction vessel and heated to 160 ° C. while stirring under atmospheric pressure. Hereinafter, a process was performed according to the same method as Experimental Example 1 to obtain a mixture including the amino acid sodium salt and / or oligo peptide salt.

<Experiment 5>

50 parts by weight of cowhide waste and 50 parts by weight of 10% aqueous calcium hydroxide solution were placed in a reaction vessel and heated to 160 ° C while stirring under atmospheric pressure. Hereinafter, a process was performed according to the same method as Experimental Example 1 to obtain a mixture including the amino acid sodium salt and / or oligo peptide salt.

<Experiment 6>

40 parts by weight of chicken waste and 60 parts by weight of 10% aqueous calcium hydroxide solution were placed in a reaction vessel and heated to 160 ° C while stirring under atmospheric pressure. Hereinafter, a process was performed according to the same method as Experimental Example 1 to obtain a mixture including the amino acid sodium salt and / or oligo peptide salt.

The type and amount of amino acids contained in the product according to Experimental Example 2 were analyzed by the amino acid analyzer, and are shown in Table 2 below. The isoelectric point of each amino acid is also shown.

In Table 2, Cys means Cystein, Asp means Aspartic acid, Glu means Glutamic acid, Ser means Serine, and Gly means Glysine. ), His means histidine, Arg means arginine, Thr means threonine, Ala means alanine, and Pro means proline. ), Tyr means Tyrosine, Val means Valine, Met means Methionine, Cys2 means two cysteines, and two disulfides And Ile means Isoleucine, Leu means Leucine, Phe means Phenylalanine, Trp means Trypsine, Lys means Lysine.

name Amount (pmol) amount(%) Isoelectric point (pH) One Cys 62.270 0.14 5.07 2 Asp 3117.782 6.98 2.77 3 Glu 4892.614 10.95 3.22 4 Ser 1610.073 3.60 5.68 5 Gly 7755.794 17.36 5.97 6 His 258.787 0.58 5.03 7 Arg 106.436 0.24 10.76 8 Thr 105.917 0.24 5.64 9 Ala 4979.645 11.15 6.00 10 Pro 6058.935 13.56 6.30 11 Tyr 548.057 1.23 5.66 12 Val 3901.818 8.74 5.96 13 Met 358.237 0.80 5.74 14 Cys2 37.397 0.08 5.07 15 Ile 1026.128 2.30 5.94 16 Leu 3881.819 8.69 5.98 17 Phe 1829.258 4.10 5.48 18 Trp 3259.581 7.30 5.98 19 Lys 877.922 1.97 9.74

Since the isoelectric points of the respective amino acids are all different, the timing at which each amino acid is formed from the amino acid salt mixture varies depending on the isoelectric point. However, since most isoelectric points of amino acids are acidic regions, metal salts should be removed from acidic regions in order to generate amino acids from amino acid salts. A more detailed description of the metal salt removal method is as follows.

First, various acids are added to amino acid salts and / or oligopeptide salts to sufficiently lower the pH to an acidic region where isoelectric points are present, preferably to a pH of about 3-4. The reaction is then cooled to a temperature range of 0-4 ° C. and centrifuged by a centrifuge while maintaining this cooling temperature. Centrifugation is performed at about 3000-4000 rpm for about 10 minutes. After about 10 minutes, pour only the supernatant and filter it again. The filtrate is dried with a spray drier at a temperature of about 120 ° C.

Hereinafter, a specific method for removing metal from each amino acid salt and / or oligopeptide salt will be described in detail with reference to Examples. First, a method for removing sodium from the sodium salt.

<Example 1>

20 parts by weight of 50% aqueous sulfuric acid solution was added to 100 parts by weight of 10% amino acid sodium salt and / or oligopeptide sodium salt as the product according to Experimental Example 2. It was cooled to about 4 ° C. and centrifuged at 3000-4000 rpm using a centrifuge while maintaining the cooling temperature. After about 10 minutes, the filtrate was filtered along the supernatant, and the resulting filtrate was dried in a spray drier at a temperature of 120 ° C. to prepare amino acids and / or oligopeptides.

<Example 2>

40 parts by weight of a 50% aqueous sulfuric acid solution was added to 100 parts by weight of 20% amino acid sodium salt and / or oligopeptide sodium salt as a product according to Experimental Example 2. Hereinafter, according to the same method as in Example 1 to prepare an amino acid and / or oligo peptide.

<Example 3>

30 parts by weight of an aqueous 30% oxalic acid solution was added to 100 parts by weight of 10% amino acid sodium salt and / or oligopeptide sodium salt as a product according to Experimental Example 2. Hereinafter, according to the same method as in Example 1 to prepare an amino acid and / or oligo peptide.

<Example 4>

60 parts by weight of a 30% aqueous oxalic acid solution was added to 100 parts by weight of 20% amino acid sodium salt and / or oligopeptide sodium salt as the product according to Experimental Example 2. Hereinafter, according to the same method as in Example 1 to prepare an amino acid and / or oligo peptide.

<Example 5>

10 parts by weight of 50% aqueous sulfuric acid solution was added to 100 parts by weight of 10% amino acid sodium salt and / or oligopeptide sodium salt as the product according to Experimental Example 2. After removing the forget-me-not, neutralized sequentially by adding 20 parts by weight of 30% aqueous oxalic acid solution again. Hereinafter, according to the same method as in Example 1 to prepare an amino acid and / or oligo peptide.

<Example 6>

20 parts by weight of 50% aqueous sulfuric acid solution was added to 100 parts by weight of 20% amino acid sodium salt and / or oligopeptide sodium salt as the product according to Experimental Example 2. After removing the forget-me-not, 40 parts by weight of 30% aqueous oxalic acid solution was added again to neutralize sequentially. Hereinafter, according to the same method as in Example 1 to prepare an amino acid and / or oligo peptide.

Table 3 shows the amount of sodium salt removed by the experiment according to Example 1-6. The amount of sodium salt was analyzed via an elemental analyzer.

Example % Of sodium removed One 90 2 94 3 90 4 96 5 96 6 98

An experiment for removing potassium from the decomposed product was performed as follows.

<Example 7>

As a product according to Experimental Example 4, 20 parts by weight of 50% aqueous sulfuric acid solution was added to 100 parts by weight of 10% amino acid potassium salt and / or oligopeptide potassium salt. Hereinafter, according to the same method as in Example 1 to prepare an amino acid and / or oligo peptide.

<Example 8>

40 parts by weight of 50% aqueous sulfuric acid solution was added to 100 parts by weight of 20% amino acid potassium salt and / or oligopeptide potassium salt as the product according to Experimental Example 4. Hereinafter, according to the same method as in Example 1 to prepare an amino acid and / or oligo peptide.

<Example 9>

30 parts by weight of 30% aqueous oxalic acid solution was added to 100 parts by weight of 10% amino acid potassium salt and / or oligopeptide potassium salt as the product according to Experimental Example 4. Hereinafter, according to the same method as in Example 1 to prepare an amino acid and / or oligo peptide.

<Example 10>

As a product according to Experiment 4, 60 parts by weight of 30% aqueous oxalic acid solution was added to 100 parts by weight of 20% amino acid potassium salt and / or oligopeptide potassium salt. Hereinafter, according to the same method as in Example 1 to prepare an amino acid and / or oligo peptide.

<Example 11>

As a product according to Experiment 4, 30 parts by weight of 30% aqueous perchloric acid solution was added to 100 parts by weight of 10% amino acid potassium salt and / or oligopeptide potassium salt. Hereinafter, according to the same method as in Example 1 to prepare an amino acid and / or oligo peptide.

<Example 12>

As a product according to Experiment 4, 60 parts by weight of 30% aqueous perchloric acid solution was added to 100 parts by weight of 20% amino acid potassium salt and / or oligopeptide potassium salt. Hereinafter, according to the same method as in Example 1 to prepare an amino acid and / or oligo peptide.

Table 4 shows the amount of potassium salt removed by the experiment according to Examples 7-12. The amount of potassium salt was analyzed by elemental analyzer.

Example Potassium removed (%) 7 90 8 94 9 91 10 96 11 94 12 96

The experiment for removing calcium from the decomposed product as in Experimental Example was performed as follows.

<Example 13>

As a product according to Experimental Example 6, 20 parts by weight of 50% aqueous sulfuric acid solution was added to 100 parts by weight of 10% amino acid calcium salt and / or oligopeptide calcium salt. Hereinafter, according to the same method as in Example 1 to prepare an amino acid and / or oligo peptide.

<Example 14>

40 parts by weight of 50% aqueous sulfuric acid solution was added to 100 parts by weight of 20% amino acid calcium salt and / or oligopeptide calcium salt as the product according to Experimental Example 6. Hereinafter, according to the same method as in Example 1 to prepare an amino acid and / or oligo peptide.

<Example 15>

30 parts by weight of a 30% aqueous oxalic acid solution was added to 100 parts by weight of 10% amino acid calcium salt and / or oligopeptide calcium salt as the product according to Experimental Example 6. Hereinafter, according to the same method as in Example 1 to prepare an amino acid and / or oligo peptide.

<Example 16>

As a product according to Experiment 6, 60 parts by weight of 30% aqueous oxalic acid solution was added to 100 parts by weight of 20% amino acid calcium salt and / or oligopeptide calcium salt. Hereinafter, according to the same method as in Example 1 to prepare an amino acid and / or oligo peptide.

<Example 17>

Carbon dioxide was injected into the product according to Experimental Example 6 to 100 parts by weight of 10% amino acid calcium salt and / or oligopeptide calcium salt until the pH was in the range of 3-4 and neutralized. Hereinafter, according to the same method as in Example 1 to prepare an amino acid and / or oligo peptide.

<Example 18>

Carbon dioxide was injected into the product according to Experimental Example 6 to 100 parts by weight of 20% amino acid calcium salt and / or oligopeptide calcium salt until the pH was in the range of 3-4 and neutralized. Hereinafter, according to the same method as in Example 1 to prepare an amino acid and / or oligo peptide.

Table 5 shows the amount of calcium salt removed by the experiment according to Example 13-18. The amount of calcium salt was analyzed by elemental analyzer.

Example Calcium removed (%) 13 96 14 99 15 98 16 99 17 99 18 99

After removing the metal from the amino acid and / or oligopeptide salt as described above, the content of the amino acid and its absorption rate are shown in Table 6.

Analysis item Analysis(%) Crude protein 68 0.2% pepsin digestibility 99.84 0.0002% pepsin digestibility 99.62 Water soluble protein 67

From Table 6, it can be seen that the amount of crude protein shows a high value close to 70%, of which most of the water-soluble protein. In addition, pepsin digestibility, which shows the degree of melting when dissolved metals and / or oligopeptides are dissolved in pepsin solutions of 0.2% and 0.0002%, is more than 99%, and the digestibility is greater than 99%, according to the present invention. Is very suitable for use as animal feed.

For comparison, the protein of 86.4% crude protein was digested by thermal hydrolysis conditions to obtain a protein, and the pepsin digestibility thereof is shown in Table 7. According to Table 7, it can be seen that the digestibility is about 50%, showing a low digestibility.

Processing conditions Pepsin digestibility (%) ² Kg / cm 2/30 bun 46.88 3 Kg / cm ^2/90 bun 62.68 4 Kg / cm ^2/120 bun 67.77 Commercially available feather powder 47.81

In order to determine the effects of amino acids and oligopeptides obtained in accordance with the method of the present invention on animals, weight gain after oral administration to 10 hamsters was obtained to obtain a weight gain and is shown in Table 8 below.

7 days later 14 days later 21 days later 28 days later Weight gain (%) Control 0.1 0.5 0.7 One Administration group 15 17 20 23

It can be seen from Table 8 that the digestibility of the amino acids and oligopeptides according to the present invention is high, so that the animal grows well through this, and thus it is very suitable as an animal feed.

The amino acids and oligopeptides according to the method of the present invention as described above are not only animal feed, fish feed, animal feed or fish feed additives, but also human nutrition, nutritional supplements, plant nutritional sources, fertilizers, fertilizers, and additives in cosmetics. Or it can use variously as an additive of surfactant.

In particular, the surfactant generally called protein shampoo is a form in which a protein such as collagen and a hydrolyzate polypeptide and amino acid are combined as a hair conditioning agent. In addition, cosmetics such as emulsion-based foundation, nourishing cream, etc. also serves as a moisturizer and nutrient source of the stratum corneum, for this purpose is prepared using a variety of amino acids. However, since the amino acids used for this purpose are expensive, they can be said to play a decisive role in the price and quality of the final product.

Since the amino acids produced according to the method of the present invention are not chemical synthesis but are natural components, it can be said that the components are complex and balanced nutrition can be provided. Therefore, excellent effects can be obtained when the amino acids of the present invention are applied to the various uses described above.

As described above, when the amino acid and the oligopeptide are produced according to the method of the present invention, various wastes and by-products can be used, which are environmentally friendly and low in manufacturing cost. In addition, since the product obtained is a natural one, when applied to a human body, an animal, a plant, etc., there is no side effect and can provide an excellent effect. When using a mixture of protein and amino acid salts and oligopeptide salts as feed, the digestibility and absorption rate of nearly 100% are guaranteed.

In addition, the amino acids and oligopeptides according to the method of the present invention are very useful for various applications where amino acids are used as raw materials.

Although the present invention has been described above according to an embodiment of the present invention, it will be apparent to those skilled in the art that various modifications may be made without departing from the spirit of the present invention.

Claims (13)

Preparing an amino acid and / or oligopeptide metal salt by performing an alkali hydrolysis reaction by adding an aqueous alkali solution to a waste or by-product having a protein content of 3% or more by weight; And A method for producing an amino acid and / or oligo peptide comprising the step of adding an acid to the metal salt to remove the metal component. The method of claim 1 wherein the waste or byproduct is a waste or byproduct obtained from a leather industry, fishery or animal husbandry activity. The method according to claim 1, wherein 40-90 parts by weight of the aqueous alkali solution is added to 5-50 parts by weight of the waste or by-product. The process of claim 1, wherein the alkaline hydrolysis is performed for about 10 minutes to 2 hours at a temperature range of about 80-170 ° C. under normal pressure or pressurized conditions in the presence of an alkaline compound. The method according to claim 4, wherein the alkaline compound is at least one aqueous solution selected from the group consisting of aqueous sodium hydroxide solution, potassium hydroxide solution and calcium hydroxide solution. The method according to claim 1, wherein the removal of the metal component is performed by adding an acid to lower and separate the pH to an acidic region where the isoelectric point of the amino acid is present. The method according to claim 6, wherein the acid is at least one selected from the group consisting of aqueous sulfuric acid solution, oxalic acid aqueous solution, carbon dioxide gas and aqueous perchloric acid solution. 7. The process according to claim 6, wherein the removal of the metal component is carried out by neutralization by addition of acid, cooling, centrifugation, filtration and drying to a temperature range of about 0-4 ° C. Amino acids and / or oligopeptides prepared according to the method of claim 1 may be added to the human nutrients, nutritional supplements, livestock feed, fish feed, animal feed or fish feed additives, plant nutritional sources, fertilizers, fertilizer additives, cosmetic additives. Or as an additive to a surfactant. The method of claim 9, wherein the metal component is removed by adding at least one acid selected from the group consisting of aqueous solution of sulfuric acid, aqueous solution of oxalic acid, carbon dioxide and aqueous solution of perchloric acid to lower the pH to an acidic region where an isoelectric point of an amino acid or oligopeptide is present. Characterized in that it is carried out by separating. The method of claim 10, wherein the removal of the metal component is carried out through neutralization by addition of acid, cooling, centrifugation, filtration and drying to a temperature range of about 0-4 ° C. 12. 10. The method of claim 9, wherein the surfactant is a shampoo. 10. The method of claim 9, wherein the cosmetic is an emulsion or nutrition cream.