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WO2006064739A1 - Method of treating biological sample for performing nucleic acid amplification - Google Patents

Method of treating biological sample for performing nucleic acid amplification

Info

Publication number
WO2006064739A1
WO2006064739A1 PCT/JP2005/022676 JP2005022676W WO2006064739A1 WO 2006064739 A1 WO2006064739 A1 WO 2006064739A1 JP 2005022676 W JP2005022676 W JP 2005022676W WO 2006064739 A1 WO2006064739 A1 WO 2006064739A1
Authority
WO
Grant status
Application
Patent type
Prior art keywords
nucleic
acid
sample
method
biological
Prior art date
Application number
PCT/JP2005/022676
Other languages
French (fr)
Japanese (ja)
Inventor
Takeshi Nagasaka
Nagahide Matsubara
Noriaki Tanaka
Original Assignee
National University Corporation Okayama University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6802General aspects
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for PCR assay

Abstract

It is intended to establish and provide a treatment method for conveniently extracting a nucleic acid in amplifying a target gene-origin nucleic acid likely contained in a biological sample without separating and purifying a nucleic acid such as a DNA from the sample by using complicated procedures as in the existing methods. Namely, a method which comprises: (a) the step of dissolving a biological sample in a buffer solution for dissolving the sample; (b) the step of impregnating a development support with the resultant solution; and (c) the step of bringing the development support into contact with a nucleic acid amplification solution. According to this method, a target gene-origin nucleic acid in a biological sample can be conveniently and efficiently amplified without resorting to complicated procedures for separating and purifying the nucleic acid.
PCT/JP2005/022676 2004-12-13 2005-12-09 Method of treating biological sample for performing nucleic acid amplification WO2006064739A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2004359468A JP2006166711A (en) 2004-12-13 2004-12-13 Simple method for amplifying feces-derived gene
JP2004-359468 2004-12-13

Publications (1)

Publication Number Publication Date
WO2006064739A1 true true WO2006064739A1 (en) 2006-06-22

Family

ID=36587795

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2005/022676 WO2006064739A1 (en) 2004-12-13 2005-12-09 Method of treating biological sample for performing nucleic acid amplification

Country Status (2)

Country Link
JP (1) JP2006166711A (en)
WO (1) WO2006064739A1 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011019505A (en) * 2009-01-29 2011-02-03 Mukogawa Gakuin Method for detecting specific gene
JP2012157295A (en) * 2011-02-01 2012-08-23 Daicel Corp Method for amplifying gene, and method for detecting specific gene

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015019952A1 (en) * 2013-08-06 2015-02-12 東洋紡株式会社 Method of amplifying nucleic acid

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4483920A (en) * 1982-05-17 1984-11-20 Hahnemann University Immobilization of message RNA directly from cells onto filter material
JPH08504081A (en) * 1992-04-01 1996-05-07 ザ ジョーンズ ホプキンズ ユニバーシティー スクール オブ メディシン Method for detecting a nucleic acid of the isolated mammalian from stool samples, and detection reagents that
JP2001221721A (en) * 2000-02-09 2001-08-17 Sapporo Imuno Diagnostic Laboratory:Kk Genetic diagnosis based on dry filter paper feces
JP2002306175A (en) * 2001-04-11 2002-10-22 Lifetech Co Ltd Method for isolating nucleic acid

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL8900725A (en) * 1989-03-23 1990-10-16 Az Univ Amsterdam The method and combination of means for isolating nucleic acid.
JP2004201607A (en) * 2002-12-26 2004-07-22 Asahi Kasei Corp Lamp reaction on nucleic acid-absorbing solid phase material

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4483920A (en) * 1982-05-17 1984-11-20 Hahnemann University Immobilization of message RNA directly from cells onto filter material
JPH08504081A (en) * 1992-04-01 1996-05-07 ザ ジョーンズ ホプキンズ ユニバーシティー スクール オブ メディシン Method for detecting a nucleic acid of the isolated mammalian from stool samples, and detection reagents that
JP2001221721A (en) * 2000-02-09 2001-08-17 Sapporo Imuno Diagnostic Laboratory:Kk Genetic diagnosis based on dry filter paper feces
JP2002306175A (en) * 2001-04-11 2002-10-22 Lifetech Co Ltd Method for isolating nucleic acid

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011019505A (en) * 2009-01-29 2011-02-03 Mukogawa Gakuin Method for detecting specific gene
JP2012157295A (en) * 2011-02-01 2012-08-23 Daicel Corp Method for amplifying gene, and method for detecting specific gene

Also Published As

Publication number Publication date Type
JP2006166711A (en) 2006-06-29 application

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