WO2006062454A1 - Prelevement d'echantillons aseptique - Google Patents

Prelevement d'echantillons aseptique

Info

Publication number
WO2006062454A1
WO2006062454A1 PCT/SE2005/001603 SE2005001603W WO2006062454A1 WO 2006062454 A1 WO2006062454 A1 WO 2006062454A1 SE 2005001603 W SE2005001603 W SE 2005001603W WO 2006062454 A1 WO2006062454 A1 WO 2006062454A1
Authority
WO
WIPO (PCT)
Prior art keywords
liquid
tube
collecting means
chamber
flow pipe
Prior art date
Application number
PCT/SE2005/001603
Other languages
English (en)
Inventor
Johan Sjöholm
Original Assignee
Tetra Laval Holdings & Finance S.A.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tetra Laval Holdings & Finance S.A. filed Critical Tetra Laval Holdings & Finance S.A.
Publication of WO2006062454A1 publication Critical patent/WO2006062454A1/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/10—Devices for withdrawing samples in the liquid or fluent state
    • G01N1/20—Devices for withdrawing samples in the liquid or fluent state for flowing or falling materials
    • G01N1/2035—Devices for withdrawing samples in the liquid or fluent state for flowing or falling materials by deviating part of a fluid stream, e.g. by drawing-off or tapping
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/10—Devices for withdrawing samples in the liquid or fluent state
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/02—Food
    • G01N33/14—Beverages
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/10—Devices for withdrawing samples in the liquid or fluent state
    • G01N1/20—Devices for withdrawing samples in the liquid or fluent state for flowing or falling materials
    • G01N1/2035—Devices for withdrawing samples in the liquid or fluent state for flowing or falling materials by deviating part of a fluid stream, e.g. by drawing-off or tapping
    • G01N2001/2071—Removable sample bottle

Definitions

  • the present invention relates to a method for determining contamination of a production system for sterile liquid food products according to the preamble of claim 1.
  • the invention further relates to a system for production of sterilised liquid food products according to the preamble of claim 7, and to a sampling device for aseptic sampling of a liquid food product flowing in a flow pipe according to the preamble of claim 12.
  • Another way of providing samples for testing is to take out samples from the liquid before it has been packaged, i.e. to connect some sort of device for taking out an amount of liquid from the system.
  • a device for withdrawal of a medium from a container is described in WO 97/16715. Using this device a number of relatively small liquid samples can be taken out from a container.
  • a commercially sterile liquid food product such as UHT milk
  • the subject of the present invention is to provide a method and a device for taking out liquid samples from a system for producing a commercially sterile liquid food product, with which device it is possible to take out both small amounts and large amounts of liquid food product for testing.
  • This subject is achieved by a method for determining contamination of a production system for commercially sterile liquid food products, said system having at least one flow pipe, through which the liquid food product flows, comprising the steps of arranging a sampling device in an opening in wall of the at least one flow pipe, said sampling device comprising a chamber and a tube which in its one end is connected to a needle-like nozzle and in its other end is connected to a collecting means, such as a bag, for collecting sampled liquid, the chamber having a elasti- cally deformable, penetrable wall which is sealingly arranged in the opening, inserting the nozzle into the flow pipe through the chamber and through the , elastically deformable, penetrable wall, passing liquid from the flow pipe to the collecting means through the
  • a peristaltic pump can be used for passing the liquid through the tube. This makes it possible to adjust the flow rate of liquid through the tube in an easy and efficient manner.
  • the production system can comprise a number of flow pipes and a number of sampling devices can be arranged in openings in walls of the flow pipes.
  • the production system further can comprise a number of packaging devices for packaging the liquid food product and the step of arranging the sampling devices in openings in walls of flow pipes can comprise arranging a respective sampling device upstream each packaging device.
  • the method can further comprise the step of filling the chamber/s with a sterilising fluid, such as alcohol or hot steam, prior to the step of inserting the nozzle.
  • a sterilising fluid such as alcohol or hot steam
  • the tube can be flexible and the passing of liquid through the tube be stopped when the amount of liquid in the collecting means has reached a predetermined level, by pressing together the tube in a stop means between an abutment and a lever, on which lever the collecting means are arranged and which abuts the tube and presses it against the abutment when the amount of liquid in the collecting means has reached the predetermined level. This makes it possible to stop the sampling automatically.
  • Fig. 1 is a schematic representation of a system for production of UHT-milk.
  • Fig. 2 shows a sampling device according to the invention.
  • Fig. 3 is a detailed, partially sectional view of the sampling device connected to a conduit.
  • Fig. 4 shows a lever arrangement for a tube in the sampling system.
  • UHT treated liquid product such as milk (UHT- milk) or fruit juice
  • Fig. 1 An exemplifying embodiment of a system for production of a liquid, commercially sterile, food product, for instance UHT treated liquid product, such as milk (UHT- milk) or fruit juice, is shown in Fig. 1.
  • UHT stands for Ultra High Temperature and UHT treatment is a well known method for making a food product commercially sterile.
  • the liquid first goes through a UHT process (sterilisation), denoted 2. Thereafter it enters an aseptic tank 3 via a flow pipe or conduit 4a. From the tank 3 the liquid is passed to three packaging devices 5a-c, via the conduits 4b and 4c.
  • the packaging devices 5a and 5b are connected in a so-called series arrangement, which means that a valve arrangement (not shown) distributes an appropriate amount of liquid to the first packaging device 5a, which produces packages filled with the liquid at a certain rate and then passes the liquid which is not distributed to this device to the second packaging device 5b, which also produces packages at a certain rate.
  • a valve arrangement (not shown) distributes an appropriate amount of liquid to the first packaging device 5a, which produces packages filled with the liquid at a certain rate and then passes the liquid which is not distributed to this device to the second packaging device 5b, which also produces packages at a certain rate.
  • a conduit 4d is arranged to recirculate the liquid, which has passed both the packaging devices 5a and 5b, without having been distributed to any of them, back to the aseptic tank.
  • This arrangement is normally used when producing a UHT-treated fruit juice. It is especially useful when one of the packaging devices is not functioning properly and therefore is not able to handle as much liquid as it normally does.
  • the packaging device 5c is instead connected to a "dead end", which means that the liquid which is not handled by the packaging device 5c is not recirculated. Instead it is discarded. This is normally the case when producing UHT-milk.
  • Three sampling devices 6a, ⁇ b and 6c are connected to the conduits 4a-c in various positions for taking out samples of liquid for analysis of the microbial content.
  • the sampling device 4a is connected to the conduit 4a for taking samples of the liquid after it has gone through the UHT-treatment, but before it enters the aseptic bath.
  • the sampling device 4b is connected to the conduit 4b to sample the liquid after it has left the aseptic tank 3, but before it has entered any of the packaging devices 5a-c.
  • sampling device 4c is connected to the conduit 4c, at a location between the two series connected packaging devices 5a and 5b, for taking out samples of the liquid right before it enters the packaging device 5b.
  • the source of contamination can be easily located with this arrangement of sampling devices at different sampling points in the production system.
  • the sampling device ⁇ comprises a hose or flexible tube 7 which in one end is connected to a bag 8 for collecting sampled liquid and in the other end to a needle-like nozzle 9, which is shown in Fig. 3.
  • the needle-like nozzle 9 is inserted from a chamber 10 into a flow pipe or conduit 4 through a membrane in the form of a elastically defor- mable, penetrable wall 11.
  • the wall 11 is sealingly arranged in an opening 12 in the conduit 4.
  • the sampling device may be retro fitted to an existing production system by exchanging part of a conduit for a conduit portion with a sampling device according to the invention where a wall 11 is sealingly arranged in an opening in a wall of the conduit portion.
  • the sampling device may also be arranged in an existing opening, wherein the wall 11 of course must be designed so that it fits accurately in the existing opening.
  • an opening may for instance be a manometer opening, which when not in use is sealed by a blind plug.
  • the wall 11 is self-sealing, so that is closes the hole made by the nozzle 9, if the end portion 9a of the needle-like nozzle 9 is pulled out of the conduit 4 through the wall and into the chamber.
  • the chamber 10 can be filled with hot steam, which is supplied to the chamber from a steam generator (not shown) , or sterilizing gas, in order to minimise the risk that any living microorganisms are present in the chamber and can enter the conduit 4 together with the nozzle end 9a as it is passed from the chamber 10 through the wall 11 and into the conduit 4.
  • a steam generator not shown
  • sterilizing gas in order to minimise the risk that any living microorganisms are present in the chamber and can enter the conduit 4 together with the nozzle end 9a as it is passed from the chamber 10 through the wall 11 and into the conduit 4.
  • the chamber can be filled with another sterilising agent such as alcohol.
  • a sterilising agent such as alcohol.
  • the fact that the chamber 10 is filled with a sterilising agent also offers protection against the effects of a possible leakage from the conduit 4 or the nozzle 9.
  • Some of liquid flowing in the conduit 4 is passed through the needle-like nozzle 9 and via the tube 7 to the bag 8, either by itself as the flowing liquid in the conduit 4 forces liquid into the nozzle, or by a peristaltic pump 13.
  • the peristaltic pump is used to adjust the volume flow rate of liquid through the tube to the bag from the conduit.
  • a throttle valve (not shown) could be arranged on the tube to adjust the volume flow rate to the bag 8 from the conduit 4.
  • the sampling device When the system is past the start-up phase, the sampling device according to the invention is instead used to continuously take out a small amount of liquid from the system at each sampling point. This is accomplished by slowing down the peristaltic pump or restricting the flow through the throttle valve and only allowing a small volume flow rate through the tube.
  • the sampling device can then be arranged to con- tinuously take out a liquid sample during a whole day. Thereby it can be verified that no contamination has occurred in the system before a specific sample point that day. If all sampling points are clear during a whole day, the only contamination source left is the respective packaging device, the testing of which is described above.
  • the hose or flexible tube 7 is according to a preferred embodiment arranged in a stop means 14 between an abutment 15 and a lever 16.
  • the lever 16 held by a holding device 17 in a position to the left of a pivot 18.
  • the holding device biases the lever in a direction away from the abutment 15, for instance by means of a spring.
  • the lever is balanced on the pivot 18, and the bag 7 rests on the lever 16 on the lever portion to the right of the pivot 18.
  • the holding device releases the lever, and the stop means then closes so that the flexible tube 7 is pressed together between the abutment 15 and the lever 16 and the flow of liquid through the tube 7 from the conduit 4 is stopped.
  • This lever and stop means arrangement could, as is apparent to a person skilled in the art, be accomplished in a number of different ways with the same result, i.e. that the liquid flow through the tube is stopped when the amount of liquid in the bag has reached a certain weight.
  • a testing equipment for performing a microbiological analysis of the content of a bag can be aseptically connected to one or more of the bags in the sampling devices. It can be arranged to take out samples of liquid from the bag in a continuous or intermittent manner.
  • a bag can be disconnected from the tube when it is filled with a desired amount of liquid and be taken away for testing by the testing equipment.
  • liquid flowing through the conduit 4, and then into the nozzle 9, to be collected in the bag 8 can contain particles of different sizes, such as is the case for a fruit juice containing pulp.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Hydrology & Water Resources (AREA)
  • Medicinal Chemistry (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

L'invention porte sur une méthode visant à déterminer la contamination d'un système de production (1) de produits alimentaires liquides biologiquement stables. Ce système comporte au moins un tuyau d'écoulement (4;4a-d) dans lequel le produit alimentaire liquide s'écoule. Cette méthode consiste à : placer un dispositif de prélèvement d'échantillons (6;6a-c) dans l'orifice d'une paroi du tuyau d'écoulement (4;4a-d) . Le dispositif de prélèvement d'échantillons (6;6a-c) comprend une chambre (10) et un tube (7) qui est raccordé, à une extrémité, à une busette (9) analogue à une aiguille et, à son autre extrémité, à un dispositif de récupération (8), tel qu'un sac, pour récupérer le liquide échantillonné. La chambre (10) possède une paroi élastiquement déformable, que l'on peut pénétrer, (11) et qui est disposée de manière hermétique dans l'orifice. La busette (9) est insérée dans le tuyau d'écoulement (4; 4a- d) par la chambre (10) et par la paroi élastiquement déformable que l'on peut pénétrer (11). Le liquide passe par le tuyau d'écoulement (4;4a-d) pour se diriger vers le dispositif de récupération (8) en passant par la busette analogue à une aiguille (9) et par le tube (7). Lorsque la quantité de liquide échantillonné se trouvant dans le dispositif de récupération (8) a atteint une valeur prédéterminée, on effectue une analyse de la contamination du liquide échantillonné et récupéré dans le dispositif de récupération (8), et en fonction des résultats de l'analyse, on détermine si les produits alimentaires obtenus pendant la durée où le liquide a traversé le sac (8) sont ou non contaminés.
PCT/SE2005/001603 2004-12-09 2005-10-26 Prelevement d'echantillons aseptique WO2006062454A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SE0402983-1 2004-12-09
SE0402983A SE0402983L (sv) 2004-12-09 2004-12-09 Aseptisk provtagning

Publications (1)

Publication Number Publication Date
WO2006062454A1 true WO2006062454A1 (fr) 2006-06-15

Family

ID=33550609

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/SE2005/001603 WO2006062454A1 (fr) 2004-12-09 2005-10-26 Prelevement d'echantillons aseptique

Country Status (2)

Country Link
SE (1) SE0402983L (fr)
WO (1) WO2006062454A1 (fr)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3776042A (en) * 1972-03-22 1973-12-04 Ladish Co Aseptic sampler fitting
US4450730A (en) * 1981-04-08 1984-05-29 Commissariat A L'energie Atomique Liquid sampler wherein solution to be sampled is used as retrievable sampler wash
US6032543A (en) * 1995-11-02 2000-03-07 Novaseptum Ab Device for introduction and/or withdrawal of a medium into/from a container
WO2002009493A1 (fr) * 2000-08-02 2002-02-07 Nl Technologies, Ltd Systeme pour collecter et isoler plusieurs echantillons steriles
US20030110870A1 (en) * 2001-12-14 2003-06-19 Bigalke Darrell Lee Continuous fluid sampler and method

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3776042A (en) * 1972-03-22 1973-12-04 Ladish Co Aseptic sampler fitting
US4450730A (en) * 1981-04-08 1984-05-29 Commissariat A L'energie Atomique Liquid sampler wherein solution to be sampled is used as retrievable sampler wash
US6032543A (en) * 1995-11-02 2000-03-07 Novaseptum Ab Device for introduction and/or withdrawal of a medium into/from a container
WO2002009493A1 (fr) * 2000-08-02 2002-02-07 Nl Technologies, Ltd Systeme pour collecter et isoler plusieurs echantillons steriles
US20030110870A1 (en) * 2001-12-14 2003-06-19 Bigalke Darrell Lee Continuous fluid sampler and method

Also Published As

Publication number Publication date
SE0402983D0 (sv) 2004-12-09
SE0402983L (sv) 2006-06-10

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