BIOCATALYST Cross-Reference to Related Applications
This application claims priority to Applicant's co-pending U.S. Provisional Application Serial No. 60/599,855 entitled "BIOCATALYST" filed August 10, 2004. The entirety of this prior patent application is incorporated herein by reference. BACKGROUND OF INVENTION Field of the Invention
This invention relates to the adaptation of U.S. Patent No. 2,908,113 issued to Martin on October 13, 1959, to a process of enhancement, concentration, dilution, sterilization, and filtration to a final product that enhances biological activity through the availability of oxygen. The conventional art has been modified by replacing seawater with fresh water. Description of the Related Art
This invention relates to the modification of U.S. Patent No. 2,903,113 issued to Martin on October 13, 1959, the contents of which are incorporated in their entirety by reference, to modify water to a catalytic active state. Its value lies in its simplicity and basic nature of the life generating capabilities of oxygen releasing water. The present invention modifies the prior art by using fresh water in place of seawater. SUMMARY OF THE INVENTION
The product, described below as a "biocatalyst", has a use, by the availability of atomic oxygen, to enhance drinking water and other beverages, lotions, gels, unguents, cosmetics, transdermal delivery systems, animal uses and spa services. This biocatalyst formed from biologically treated water acts as a biocatalyst to replace the water components of the above list and to enhance the water used in fish farming, aquaculture, or mariculture.
A biocatalyst is a substance that initiates or modifies the rate of a biological process. The biocatalyst stimulate biological activity when the biocatalyst molecule contacts a cell wall, inducing a catalytic reaction which causes the oxygen atom to be released from the biocatalyst directly to the cell. Through a unique process of modification and filtration, the biocatalyst of the present invention allows for availability of atomic oxygen and is rendered potable and shelf stable. This catalytically enhanced water can be used as part of a water component or to replace water, in order to provide added benefits by improving health and endurance, reducing disease, and improving the efficiency of farming.
Additional aspects, features, and advantages of the invention will be set forth in the description which follows, and in part will be apparent from the description, or may be learned by practice of the invention. The aspects, features, and advantages of the invention
will Ie 1V^e Il iέefr1 'Ift'cJ'Saf n Id ^/4' hi 'ϊtr u ctu re and steps particularly pointed out in the written description, the claims, and the drawings. BRIEF DESCRIPTION OF THE DRAWINGS
Figure 1 shows a production process for using the product of conventional art to produce making the biocatalyst of the present invention through modification and filtration. DETAILED DESCRIPTION OF INVENTION
As shown in Figure 1 , regular water is added to a first container where it contacts a biomass 6. The inflow of water into the first container causes an outflow of biologically treated water 8 from the top of the first container or biogenerator 2 at a rate equal to the inflow of water. The biologically treated water 8 from the biogenerator 2 flows into a second container or mixture/storage tank 4, where additional water is added. The mixture/storage tank 4 contains biologically treated water 8 from the biogenerator 2 diluted with regular water at a ratio of about 1.5 to about 8, preferably about 4, parts regular water to about 1 part of the biologically treated water to form the biocatalyst 20. The biocatalyst 20 then is removed from the bottom of the mixture/storage tank 4 and filtered through a filtration system 18.
The water added to the biogenerator and mixture/storage tanks 4 can be, for example, fresh or clean water that is free from metals, such as heavy metals, toxins, or other contaminants. In one embodiment of the present invention, the water contains less that 10 ppm of metal ions. In another embodiment of the present invention, the water contains less that 5 ppm of metal ions. In another embodiment of the present invention, the water contains less than 1 ppm of metal ions. In another embodiment of the present invention, the water contains an amount of metal ions that are not detectable by conventional instruments.
As shown in the Figure, water entering the biogenerator 2 passes through a nozzle
14. The nozzle 14 in the biogenerator 2 ensures that the water entering the biogenerator 2 creates a vortex which provides for optimum contact with the biomass 6 at the bottom of the biogenerator 2.
The biomass 6 can comprise excreta from lactating ruminants, such as cows. In one example of the present invention, five pounds of biomass 6 in the form of a dry powder can be deposited in the bottom of the biogenerator 2. Then, clean water is poured into the biogenerator 2 and where it contacts the biomass 6, whereby the hydrogen and oxygen bond of the water are altered by the biomass 6, resulting in the biologically treated water. The biologically treated water 8 is allowed to overflow out through the top of the biogenerator discharge 16 into the mixture/storage tank 4. In the process, some of the biomass 6 may be lost. Thus, in order to replace biomass 6 lost through the outflow of biologically treated water 8 from the top of the biogenerator 2, one ounce of biomass 6 can be added through
the
hydrate the added biomass 6 when it enters the biogenerator 2.
During the process, some of the biomass 6 may rise to the surface of the biologically treated water 8 in the biogenerator 2. The rising of the biomass 6 occurs because portions of the biomass 6 become full of trapped bubbles. Any floating biomass 6 can simply be compressed by hand or mechanical device, under the surface of the water in the biogenerator 2, to release the trapped bubbles. As a result, the biomass portions 6 are no longer buoyant and can sink back to the bottom of the biogenerator 2.
The rate of flow of water through the flow meter 10 into the biogenerator 2 can be between 20 gallons per hour (gph) and 40 gph. In one embodiment of the present invention, the flow of water into the biogenerator 2 is not less than 20 gallons per hour (gph) and not more than 40 gph. The rate of flow of water through the flow meter 12 into the mixture/storage tank 4 is dependent on the rate of flow into the biogenerator 2. For example, the rate of flow of water through the flow meter 12 into the mixture/storage tank 4 can be four times greater than the rate of flow of water into the biogenerator 2. Thus, if the rate of flow of water into the biogenerator 2 is 40 gph, the rate of flow into the mixture/storage tank 4 is set to 160 gph to maintain the ratio of about 4 parts regular water to about 1 part of the biologically treated water.
If the ratio is not maintained, for example, and a greater amount of water is used to dilute the biologically treated water, then the beneficial properties of the biologically treated water will be diluted. If, on the other hand, less water is used to dilute the biologically treated water, the covalent bond of the hydrogen and oxygen molecules will strengthen and the biologically treated water will revert to the regular or ordinary water. For example, regular water can be added to a 55-gallon biogenerator 2 at a rate of 0.33 to 0.66 gallons per minute (gpm), and flow into the mixture/storage tank 4 at the same rate. Fresh water for dilution can be added to the mixture/storage tank 4 at a rate of four times the flow rate of water into the biogenerator 2.
The rate of removal of the biocatalyst from the mixture/storage tank 4 is the combined flow rate of the fresh water and the raw biologically treated water. As such, the rate of flow of fresh water into the biogenerator 2 causes the biologically treated water to flow out of the biogenerator 2 into the mixture/storage tank 4 at exactly the same rate. In one embodiment of the present invention, the rate of removal of biologically treated water can be five times the flow rate into the biogenerator 2. Due to attrition, about 1 ounce per day of biomass 6 is added to the biogenerator 2, five days a week.
size and shape. In one example of the present invention, the biogenerator 2, is cylindrical, 55-gallon container, with the diameter approximately 2/3 the height. The cylindrical shape of the container allows for an efficient flow of water from the nozzle 14 down to the biomass 6 and up toward the top of the container, so that the resulting biologically treated water can efficiently pass from the biogenerator 2 to the mixture/storage tank 4. The biogenerator 2 and mixture/storage tanks 4 which can be formed from a polymeric material such as, for example, high-density polyethylene (HDPE) or polyvinyl chloride (PVC). Other examples of materials for the containers include ceramics and glass. In one embodiment of the present invention, the biogenerator 2 is preferably non-metal. Alternatively, if a metal is used, it is coated to prevent contact with the metal, preferably coated with an epoxy. Similarly, all related materials that are in contact with the biologically treated water are also either non-metal or coated metals.
The mixture/storage tank 4 contains the biologically treated water plus four times that volume in diluent water, thus, the storage tank is larger than the biogenerator 2.
The filtration process involves a unique multi-stage system 18 designed to ensure potability and shelf-life stability of the biocatalyst. Filtration is multi-stage process that includes filtering the biocatalyst through an initial three stages using poly filters. In the first stage, a 5-μ nominal filtration poly filter removes gross contaminants. In the second stage, a 0.35-μ nominal filtration poly filter is used. In the third stage, a 0.2 μ absolute filtration poly filter performs absolute filtration and ensures that no pathogens such as e coli, giardia, pseudomonas, or the like, can pass therethrough. In the fourth stage of the filtration process, the biocatalyst is exposed to ultraviolet radiation. The UV lights can be sized based on the predetermined flow rate of the biocatalyst, so that the UV units can irradiate the delivered volume of biocatalyst. In the last stage of the filtration process, the biocatalyst is filtered through activated charcoal or a carbon block to improve taste and remove odor.
Ceramic filters, 0.6 microns or finer, may be used in lieu of standard replaceable filter elements. A reverse osmosis filtration system can be used when large volumes of the biocatalyst must be purified. After ceramic or reverse osmosis filtration, the product will be treated with UV-irradiation and filtered with activated charcoal. Reverse osmosis systems incorporate pre-filtration consisting of one or more back-flushable media tanks, one or more back-flushable activated charcoal tanks, and UV-irradiation. The pre-treatment is to ensure that no bacteria gather or grow on the reverse osmosis membranes and to ensure the removal of contaminants such as heavy metals, iron, calcium, and chlorine. As mentioned
above,1 after felv?rll!!^έ'rτfdlsft1reltfnent, the product will again be treated with UV and activated charcoal.
As a result of the contact between the water and the biomass 6, the water molecule undergoes a reaction whereby the bond between the hydrogen and oxygen atoms is altered. Specifically, the bond between the hydrogen and oxygen atoms of water is altered so that the atoms can be released upon contact with a cell wall. Thus, the biologically treated water of the present invention is chemically/physically identical to water except that the surface tension is significantly lower. Specifically, the biocatalyst has a surface tension of about 52 to 65, preferably 61 dynes/cm compared to 72.75 dynes/cm for standard water. In addition, the biologically treated water of the present invention freezes at about 33°F to 35° F, preferably about 340F, compared to 320F for standard water. The biologically treated water is unstable, until it is diluted to form the biocatalyst. After 24 hours, the biologically treated water begins to revert to ordinary water. In particular, the surface tension will revert to 72.75 dynes/cm2 and the freezing point will lower to 32° F. The process produces an all-natural product, a biocatalyst of which the direct action is unknown. Indirectly, by best scientific analogy, the process somehow alters the molecular structure of water by catalytically breaking down the water molecule to small amounts of atomic oxygen atoms. This is suggested by new NASA results in archival research of the role of water in the initiation of life. Extensive research has shown that the biologically treated water action to produce oxygen atoms can enhance microbial populations by three orders of magnitude, allowing biological oxidation in anaerobic conditions. In waste treatment systems it can double the biological activity reducing sludge and biological oxygen demand (BOD) output.
This availability of atomic oxygen has value as the oxygen atoms enhance medicinal treatment of burns, skin damage, etc. as described below. In addition, the biocatalyst can be used in place of water in special sports and energy beverages used by athletes and others in preparation, endurance, and recovery from activities that strain the body such as marathons, iron man competitions, or any other strenuous activity. The biocatalyst enhances the benefits of such drinks. This invention also relates to the use of biologically treated water in eyewashes, topical applications, cosmetics, spa products, and animal additives.
The invention further relates to the use of biologically treated water for topical application.
The invention further relates to a method of treating asthma, emphysema, lung damage, sports recovery, and hangovers by ingesting biologically treated water.
The invention further 'relates "to a method of treating redness and eye fatigue, nasal irritation, burns, wrinkles, hair loss, shingles, eczema, acne, stretch marks, scar tissue, and hemorrhoids by topical or ocular application of a liquid, lotion, gel, or unguent containing biologically treated water. The invention further relates to a method of transdermal delivery by dissolving a pharmaceutical product in biologically treated water before applying topically.
The invention further relates to liquid products for human or animal consumption having all or any part of the water component replaced by biologically treated water.
The invention further relates to liquid, gel, unguent, or cosmetic products for topical application to people having all or any part of the water component replaced by biologically treated water.
The invention further relates to the replacement of water or a portion of water used in hot tubs, misting cabinets, and other spa services with biologically treated water.
United States Patent Nos. 3,227,648 (Hahn et al., issued January 4, 1966); 3,362,905 (Gleave, issued January 9, 1968); 3,431 ,675 (Moore, issued March 1 1 , 1969); 3,456,812
(Masayasu et al., issued December 15, 1970); and 3,855,121 (Gough, issued December 17,
1974), the contents of all of which are incorporated in their entirety by reference, teach variations on the basic method where yeast or another fungus can be added to speed the fermentation. Also, seawater can be used to speed the turning process, where the solids rise to the top of the mixture before removal. The biologically treated water can be produced without seawater if other factors are optimized. Protozoa inhibitors such as calcium carbide may be added, as well as other additives such as casein of milk. Regulation of the temperature of the mixture, the pH levels, and the ratio of the height of solid matter to liquid can be optimized as taught in the referenced patents. Non-limiting examples of uses of the present invention are described as follows.
I. Drinks
The finished biologically treated water can be used in place of any part of the water in drinks. The biologically treated water provides enhanced benefits when consumed as bottled water. The biologically treated water can also be used in place of water in fruit based drinks, ready-to-drink teas, coffee products, soft drinks, sport drinks, drinks with electrolyte replenishers, and energy drinks. The biologically treated water increases SpO2 levels for people with asthma, emphysema, or other lung damage. This increase can easily be measured using a pulse oximeter. In order to benefit from such increases, drinks containing the present invention are ingested as needed.
'6i(5to'gϊcaiiy" 1rdateαr""wϊitlr can be used in place of water in special sports beverages used by athletes in preparation, endurance, and recovery from activities that strain the body such as marathons or iron man competitions. The biologically treated water enhances the benefits of such drinks. The biologically treated water can be used in place of all or part of the water component in the preparation of beer, wine, and other alcoholic beverages.
The biologically treated water can be used to replace water in cold brewing green tea. The resulting product provides all the benefits of green tea with the enhanced qualities of the biologically treated water and can be used as an aid for hangovers. Undiluted cold brewed green tea made with the present invention may be consumed before sleeping to reduce the effects of a hangover in a preventative manner. The product can also be consumed to provide increased relief from the effects of an existing hangover.
One embodiment of the present invention is a present method of treating one of asthma, emphysema, lung damage, or hangovers by ingesting a product comprising biologically treated water.
Another embodiment of the present invention is a method of treating fatigue related to sports activities by ingesting a product comprising biologically treated water.
Another embodiment of the invention is liquids to be consumed by humans or animals comprising biologically treated water. Another embodiment of the present invention is green tea brewed with biologically treated water.
Another embodiment of the present invention is a drink for consumption by humans wherein the improvement comprises replacing at least a part of the water component with biologically treated water. II. Eyewashes and Nasal Sprays
The biologically treated water of the present invention can be used in place of at least a part of water in saline solutions prepared for application to eyes. An isotonic (0.9%) saline solution prepared with the present invention provides all the benefits of typical saline eyewash with increased reduction of redness and increased relief of eye fatigue. The isotonic saline solution prepared with the present invention is applied topically to the eye. Eyewashes containing the present invention speeds healing time for eye injuries associated with windbum, sunburn, dust and pollution. Additionally, eyewashes containing the present solution to speed recovery from LASIK eye surgery compared to ordinary eye drops.
Similar to the replacement of the water content in saline solutions for the eye, the biologically treated water of the present invention can be used in place of all or part of the
used in nasal sprays. A nasal spray using a mild saline solution including the present invention soothes irritated nasal passages with or without additional additives. The saline solution also speeds recovery of irritated nasal passages with or without additional additives. An embodiment of the present invention is a method of treating irritated eyes by applying a fluid containing biologically treated water.
Another embodiment of the present invention is a method of treating eye fatigue by applying a fluid containing biologically treated water.
Another embodiment of the present invention is a method of treating any one of windbum, sunburn, irritation from dust, irritation from pollution, and irritation due to laser surgery in the eye by applying a fluid containing biologically treated water to the eye.
Another embodiment of the present invention is a method of treating irritated nasal passages by applying a product containing biologically treated water.
Another embodiment of the present invention is a method of treating irritated nasal passages by applying a product containing biologically treated water and another additive.
Another embodiment of the present invention is a solution to be applied to the eye, wherein the improvement comprises replacing any part of the water component with biologically treated water.
Another embodiment of the present invention is a solution to be applied to the nasal passage, wherein the improvement comprises replacing any part of the water component with biologically treated water. III. Products for Topical Application
The biologically treated water of the present invention can be used to replace all or part of the water component in products intended to be applied topically to humans or animals. The present invention can be used in place of at least a part of the water component of various lotions, gels, unguents or cosmetic product.
A liquid or gel substance containing the present invention heals burns significantly faster than other treatments. This product can speed healing beyond the topical application of aloe vera. Additional ingredients can also be added to the gel or liquid such as menthol or cold brewed green tea. However, the product is effective with or without these additives. The product can also be applied topically several times daily. For example, the product can be applied 2-6 times daily until the burn is healed.
A liquid or gel substance containing the present invention can also be applied topically once or several times daily to treat shingles, eczema, acne, stretch marks, scar tissue and hemorrhoids. For example, the product can be applied 2 to 6 times daily. As with
the
is beneficial whether the biologically treated water of the present invention replaces all or a part of the water component of such liquids or gels. Additional ingredients may be added to the lotion or gel. For example green tea, green tea extracts, menthol, eucalyptus, or aloe vera can be added to the lotion or gel along with biologically treated water.
A gel containing the present invention speeds healing of bruises as well as reduces swelling, pain and itching associated with hemorrhoids. A product containing the present invention in a liquid, lotion, gel or other form speeds the healing of cold sores. A gel form of the present invention can also be used in products as an intimate lubricant. Continued application of a gel containing the biologically treated water of the present invention results in a reduction of wrinkles and fine lines. The gel containing the present invention as a replacement for all or part of the water component can be applied topically each day. Over a period of time, a reduction in the appearance of fine lines and wrinkles occurs as skin cells appear more hydrated and oxygenated creating a healthier and more elastic appearance. Application of the product can be continual (i.e., every day for a period of at least 2-100 days) or intermittent. In a preferred embodiment of the invention, the application is continual.
A similar daily, topical application of a gel containing the biologically treated water of the present invention to the scalp can aid in regrowth and retention of hair when the cause of hair loss is reduced blood flow to the scalp. Application of the product can be continuous or intermittent in order to encourage regrowth and retention of hair. In a preferred embodiment of the invention, regular application to the scalp is made on a continuing basis.
An embodiment of the present invention is a method of treating burns by applying a substance containing biologically treated water topically. Another embodiment of the present invention is a method of treating shingles by applying a substance containing biologically treated water topically.
Another embodiment of the present invention is a method of treating eczema by applying a substance containing biologically treated water topically.
Another embodiment of the present invention is a method of treating acne by applying a substance containing biologically treated water topically.
Another embodiment of the present invention is a method of treating stretch marks by applying a substance containing biologically treated water topically. ■
Another embodiment of the present invention is a method of treating scar tissue by applying a substance containing biologically treated water topically.
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Another" e"mDoαιήnet1t"bf""th'e" present invention is a method of treating any one of swelling, itching, or pain caused by hemorrhoids by applying a substance containing biologically treated water topically.
Another embodiment of the present invention is a method of treating cold sores by applying a substance containing biologically treated water topically.
Another embodiment of the present invention is a method of treating bruises by applying a substance containing biologically treated water topically.
Another embodiment of the present invention is a method of reducing wrinkles by applying a substance containing biologically treated water topically. Another embodiment of the present invention is a method of treating hair loss by applying a substance containing biologically treated water topically.
Another embodiment of the present invention is a gel, wherein the improvement comprises replacing any part of the water component with biologically treated water.
Another embodiment of the present invention is a lotion, wherein the improvement comprises replacing any part of the water component with biologically treated water.
Another embodiment of the present invention is an unguent, wherein the improvement comprises replacing any part of the water component with biologically treated water. IV. Transdermal Delivery Systems
The biologically treated water of the present invention can be used in the transdermal delivery of pharmaceutical products. The biologically treated water of the present invention is mixed with the chosen product in a liquid or gel form to provide a transdermal delivery system. Such pharmaceutical products can be methyl sulfonyl methane; aspirin; pharmaceutical and herbal products such as sera for immunization; analgesics; birth control and hormonal applications; veterinary applications such as immunizations, flea, tick and heartworm prevention; antibiotics; and others. The inclusion of the present invention in such transdermal delivery systems enhances the quick and painless penetration of the pharmaceutical product to provide delivery directly to the affected area. The biologically treated water of the present invention may act as a transdermal delivery system for any additive dissolved in it. An embodiment of the present invention is a method of making a transdermal delivery system, comprising dissolving a pharmaceutical product in biologically treated water.
Another embodiment of the present invention is a transdermal delivery system comprising biologically treated water.
Another embodiment of the present invention is a transdermal delivery system comprising biologically treated water and methyl sulfonyl methane.
" Another"1 δlribd'diήne'nr '1O1Ih tf16Apresent invention is a transdermal delivery system comprising biologically treated water and aspirin.
Another embodiment of the present invention is a transdermal delivery system comprising biologically treated water and herbal products. Another embodiment of the present invention is a transdermal delivery system comprising biologically treated water and sera for immunization.
Another embodiment of the present invention is a transdermal delivery system comprising biologically treated water and analgesics.
Another embodiment of the present invention is a transdermal delivery system comprising biologically treated water and birth control.
Another embodiment of the present invention is a transdermal delivery system comprising biologically treated water and hormonal applications.
Another embodiment of the present invention is a transdermal delivery system comprising biologically treated water and immunizations for veterinary uses. Another embodiment of the present invention is a transdermal delivery system comprising biologically treated water and flea prevention products.
Another embodiment of the present invention is a transdermal delivery system comprising biologically treated water and tick prevention products.
Another embodiment of the present invention is a transdermal delivery system comprising biologically treated water and heartworm prevention products.
Another embodiment of the present invention is a transdermal delivery system comprising biologically treated water and an antibiotic.
Another embodiment of the present invention is a transdermal delivery system, wherein the improvement comprises replacing any part of the water component with biologically treated water.
Another embodiment of the present invention is a method of method of applying pharmaceutical products transdermal^ comprising the step of dissolving the pharmaceutical product in biologically treated water. V. Cosmetics The biologically treated water of the present invention can be used as a gel, lotion, or unguent as part of a cosmetic product. The present invention may also be used in all types of cosmetics to replace all or any part of the water component. The present invention enhances hydration and oxygenation. For example, the present invention can be used as a component of shaving gel for men or women. The shaving gel including the biologically treated water not only lubricates but also aids healing of minor nicks and cuts.
""' m embϋdtmaϊf d'f i!Fiy::pli's8!πfinvention is a cosmetic product comprising biologically treated water.
Another embodiment of the present invention is a method of making a cosmetic product with the step of replacing any part of the water component with biologically treated water.
Another embodiment of the present invention is a cosmetic product, the improvement comprising the replacement of any part of the water component with biologically treated water.
Another embodiment of the present invention is shaving gel comprising biologically treated water.
Another embodiment of the present invention is the treatment of minor nicks and cuts caused by shaving by the application of a gel comprising biologically treated water. Vl. Spa Products
The biologically treated water can be used in place of all or any part of the water used in hot tubs, misting cabinets, or any other spa application where water is used. Use of the present invention in place of water provides enhanced hydration and oxygenation of all exposed skin surfaces.
Another embodiment of the present invention is the treatment of skin surfaces by contact with biologically treated water. VII. Animals
The biologically treated water of the present invention can also be used in place of all or any part of regular water used with animals, whether pets or livestock. The present invention aids in pet health when used in place of all or a part of water as a drink for dogs, cats, birds, small animals, and other pets. The biologically treated water of the present invention can be used in place of all or any part of the water added to aquariums. This use increases the health of fish in the aquarium. The present invention can be used in place of all or any part of the water component used in fish farming, aquaculture, or mariculture. This use provides for increased weight gain, growth, and reduced feed costs. Further benefits include decreased time to market and increased general health of stock. The benefits are increased in high- density applications.
The biologically treated water of the present invention can be used to replace all or a part of the drinking water for animals. The present invention, when used to replace the drinking water for racehorses and greyhounds, enhances the animals' ability to run. Increases are seen both in the animals' speed and endurance. The biologically treated
,™ ,.„„ ,„„,„ ... J „ „.,.,, „.,„ W::, r,, p .„„ p „,.„, water c'aVbe useα tbTepla'c'e BFcir any part of the drinking water for livestock such as cattle, pork, sheep, goats, and all types of poultry. This use lessens the amount of time to market, reduces the amount of necessary feed, and increases weight of the treated animals.
An embodiment of the present invention is a method of raising animals the improvement comprising replacing any part of the animals drinking water with biologically treated water.
Another embodiment of the present invention is a method of fish farming the improvement comprising the step of replacing any part of the water component with biologically treated water. Another embodiment of the present invention is a method of aquaculture the improvement comprising the step of replacing any part of the water component with biologically treated water.
Another embodiment of the present invention is a method of mariculture, wherein the improvement comprises replacing any part of the water component with biologically treated water.
It is to be understood that while the invention has been described above using specific embodiments, the description and examples are intended to illustrate the structural and functional principles of the present invention and are not intended to limit the scope of the invention. On the contrary, the present invention is intended to encompass all modifications, alterations, and substitutions within the spirit and scope of the disclosure.