WO2005121358A3 - Extendable probes - Google Patents

Extendable probes Download PDF

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Publication number
WO2005121358A3
WO2005121358A3 PCT/DK2005/000383 DK2005000383W WO2005121358A3 WO 2005121358 A3 WO2005121358 A3 WO 2005121358A3 DK 2005000383 W DK2005000383 W DK 2005000383W WO 2005121358 A3 WO2005121358 A3 WO 2005121358A3
Authority
WO
WIPO (PCT)
Prior art keywords
probes
extendable
extendable probes
pcr
relates
Prior art date
Application number
PCT/DK2005/000383
Other languages
French (fr)
Other versions
WO2005121358A2 (en
Inventor
Henrik M Pfundheller
Michael Meldgaard
Peter Mouritzen
Peter Stein Nielsen
Mikkel Noerholm
Soeren Morgenthaler Echwald
Original Assignee
Exiqon As
Henrik M Pfundheller
Michael Meldgaard
Peter Mouritzen
Peter Stein Nielsen
Mikkel Noerholm
Soeren Morgenthaler Echwald
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Exiqon As, Henrik M Pfundheller, Michael Meldgaard, Peter Mouritzen, Peter Stein Nielsen, Mikkel Noerholm, Soeren Morgenthaler Echwald filed Critical Exiqon As
Publication of WO2005121358A2 publication Critical patent/WO2005121358A2/en
Publication of WO2005121358A3 publication Critical patent/WO2005121358A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention relates to probes which are extendable useful as PCR probes and in probe libraries. The invention further relates to prevention of replication of a primer extension product in PCR reactions.
PCT/DK2005/000383 2004-06-10 2005-06-10 Extendable probes WO2005121358A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA200400907 2004-06-10
DKPA200400907 2004-06-10

Publications (2)

Publication Number Publication Date
WO2005121358A2 WO2005121358A2 (en) 2005-12-22
WO2005121358A3 true WO2005121358A3 (en) 2006-03-23

Family

ID=35482321

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK2005/000383 WO2005121358A2 (en) 2004-06-10 2005-06-10 Extendable probes

Country Status (1)

Country Link
WO (1) WO2005121358A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8034569B2 (en) * 2008-06-06 2011-10-11 Biotex, Inc. Methods for molecular detection

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001009376A1 (en) * 1999-07-29 2001-02-08 Cytocell Limited Method for amplification of nucleic acids
US20030113765A1 (en) * 1999-10-26 2003-06-19 Epoch Biosciences, Inc. Hybridization-triggered fluorescent detection of nucleic acids
WO2004113563A2 (en) * 2003-06-20 2004-12-29 Exiqon A/S Probes, libraries and kits for analysis of mixtures of nucleic acids and methods for constructing the same
WO2005003373A2 (en) * 2003-06-26 2005-01-13 Proligo, Llc Fluorogenic nucleic acid probes including lna for methods to detect and/or quantify nucleic acid analytes

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001009376A1 (en) * 1999-07-29 2001-02-08 Cytocell Limited Method for amplification of nucleic acids
US20030113765A1 (en) * 1999-10-26 2003-06-19 Epoch Biosciences, Inc. Hybridization-triggered fluorescent detection of nucleic acids
WO2004113563A2 (en) * 2003-06-20 2004-12-29 Exiqon A/S Probes, libraries and kits for analysis of mixtures of nucleic acids and methods for constructing the same
WO2005003373A2 (en) * 2003-06-26 2005-01-13 Proligo, Llc Fluorogenic nucleic acid probes including lna for methods to detect and/or quantify nucleic acid analytes

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
KUTYAVIN I V ET AL: "3'-minor groove binder-DNA probes increase sequence specificity at PCR extension temperatures", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 28, no. 2, 15 January 2000 (2000-01-15), pages 655 - 661, XP002318952, ISSN: 0305-1048 *
LATORRA D ET AL: "Design considerations and effects of LNA in PCR primers", MOLECULAR AND CELLULAR PROBES, ACADEMIC PRESS, LONDON, GB, vol. 17, no. 5, October 2003 (2003-10-01), pages 253 - 259, XP004466447, ISSN: 0890-8508 *
LETERTRE C ET AL: "Evaluation of the performance of LNA and MGB probes in 5'-nuclease PCR assays", MOLECULAR AND CELLULAR PROBES, ACADEMIC PRESS, LONDON, GB, vol. 17, no. 6, December 2003 (2003-12-01), pages 307 - 311, XP004471011, ISSN: 0890-8508 *

Also Published As

Publication number Publication date
WO2005121358A2 (en) 2005-12-22

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