WO2006074351A3 - Reversible nucleotide terminators and uses thereof - Google Patents

Reversible nucleotide terminators and uses thereof Download PDF

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Publication number
WO2006074351A3
WO2006074351A3 PCT/US2006/000432 US2006000432W WO2006074351A3 WO 2006074351 A3 WO2006074351 A3 WO 2006074351A3 US 2006000432 W US2006000432 W US 2006000432W WO 2006074351 A3 WO2006074351 A3 WO 2006074351A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleotide terminators
reversible nucleotide
reversible
terminators
useful
Prior art date
Application number
PCT/US2006/000432
Other languages
French (fr)
Other versions
WO2006074351A2 (en
Inventor
Douglas Smith
Kevin Mckernan
Alan Blanchard
Original Assignee
Agencourt Personal Genomics
Douglas Smith
Kevin Mckernan
Alan Blanchard
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agencourt Personal Genomics, Douglas Smith, Kevin Mckernan, Alan Blanchard filed Critical Agencourt Personal Genomics
Priority to EP06733635A priority Critical patent/EP1879906A4/en
Priority to JP2007550492A priority patent/JP2008526877A/en
Publication of WO2006074351A2 publication Critical patent/WO2006074351A2/en
Publication of WO2006074351A3 publication Critical patent/WO2006074351A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/073—Pyrimidine radicals with 2-deoxyribosyl as the saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention provides nucleotide analogs which are useful in one or more DNA sequencing techniques.
PCT/US2006/000432 2005-01-05 2006-01-05 Reversible nucleotide terminators and uses thereof WO2006074351A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP06733635A EP1879906A4 (en) 2005-01-05 2006-01-05 Reversible nucleotide terminators and uses thereof
JP2007550492A JP2008526877A (en) 2005-01-05 2006-01-05 Reversible nucleotide terminator and use thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US64154905P 2005-01-05 2005-01-05
US60/641,549 2005-01-05

Publications (2)

Publication Number Publication Date
WO2006074351A2 WO2006074351A2 (en) 2006-07-13
WO2006074351A3 true WO2006074351A3 (en) 2007-01-18

Family

ID=36648200

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/000432 WO2006074351A2 (en) 2005-01-05 2006-01-05 Reversible nucleotide terminators and uses thereof

Country Status (3)

Country Link
EP (1) EP1879906A4 (en)
JP (1) JP2008526877A (en)
WO (1) WO2006074351A2 (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9146248B2 (en) 2013-03-14 2015-09-29 Intelligent Bio-Systems, Inc. Apparatus and methods for purging flow cells in nucleic acid sequencing instruments
US9228228B2 (en) 2006-10-27 2016-01-05 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
US9334490B2 (en) 2006-11-09 2016-05-10 Complete Genomics, Inc. Methods and compositions for large-scale analysis of nucleic acids using DNA deletions
US9476054B2 (en) 2005-06-15 2016-10-25 Complete Genomics, Inc. Two-adaptor library for high-throughput sequencing on DNA arrays
US9591268B2 (en) 2013-03-15 2017-03-07 Qiagen Waltham, Inc. Flow cell alignment methods and systems

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8445194B2 (en) 2005-06-15 2013-05-21 Callida Genomics, Inc. Single molecule arrays for genetic and chemical analysis
EP2495337A1 (en) 2006-02-24 2012-09-05 Callida Genomics, Inc. High throughput genome sequencing on DNA arrays
US11940413B2 (en) 2007-02-05 2024-03-26 IsoPlexis Corporation Methods and devices for sequencing nucleic acids in smaller batches
WO2009029728A1 (en) 2007-08-29 2009-03-05 Applied Biosystems Inc. Alternative nucleic acid sequencing methods
CN101910399B (en) 2007-10-30 2015-11-25 考利达基因组股份有限公司 For the device of high throughput sequencing of nucleic acids
DK2610351T3 (en) 2007-12-05 2015-09-28 Complete Genomics Inc Base efficient provision of sequencing reactions
US9085798B2 (en) 2009-04-30 2015-07-21 Prognosys Biosciences, Inc. Nucleic acid constructs and methods of use
DK2977455T3 (en) 2009-06-15 2020-07-13 Complete Genomics Inc PROGRESS FOR LONG-FRAGMENT READING SEQUENCE
KR101107315B1 (en) * 2009-09-29 2012-01-20 한국과학기술연구원 A DNA sequencing method using novel nucleoside triphosphates with a fluorescent 3'-O-blocking group as reversible terminators
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
PT2556171E (en) 2010-04-05 2015-12-21 Prognosys Biosciences Inc Spatially encoded biological assays
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
US9453258B2 (en) 2011-09-23 2016-09-27 Illumina, Inc. Methods and compositions for nucleic acid sequencing
US10837879B2 (en) 2011-11-02 2020-11-17 Complete Genomics, Inc. Treatment for stabilizing nucleic acid arrays
EP2894158B1 (en) 2012-09-04 2018-06-20 Kabushiki Kaisha DNAFORM Compound, nucleic acid, labeling substance, and detection method
LT3013983T (en) 2013-06-25 2023-05-10 Prognosys Biosciences, Inc. Spatially encoded biological assays using a microfluidic device
JP6261027B2 (en) * 2013-07-23 2018-01-17 国立大学法人東京工業大学 2'-O-carbamoyl modified nucleoside triphosphates
EP2876166B1 (en) * 2013-11-20 2016-12-14 Roche Diagnostics GmbH New compound for sequencing by synthesis
CN113186256A (en) 2015-04-10 2021-07-30 空间转录公司 Spatially differentiated, multiplexed nucleic acid analysis of biological samples
US11591647B2 (en) 2017-03-06 2023-02-28 Singular Genomics Systems, Inc. Nucleic acid sequencing-by-synthesis (SBS) methods that combine SBS cycle steps
WO2019067635A1 (en) * 2017-09-27 2019-04-04 Ultima Genomics, Inc. Methods and systems for nucleic acid sequencing

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5852182A (en) * 1990-01-11 1998-12-22 Isis Pharmaceuticals Inc. Thiol-derivatized oligonucleosides
US6265588B1 (en) * 1997-01-28 2001-07-24 Aventis Pharma Deutschland Gmbh Isoxazole and crotonamide derivatives and their use as pharmaceuticals and diagnostics

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6087482A (en) * 1990-07-27 2000-07-11 Isis Pharmaceuticals, Inc. Heteroatomic oligonucleoside linkages
US5646269A (en) * 1994-04-28 1997-07-08 Gilead Sciences, Inc. Method for oligonucleotide analog synthesis
SE9500342D0 (en) * 1995-01-31 1995-01-31 Marek Kwiatkowski Novel chain terminators, the use thereof for nucleic acid sequencing and synthesis and a method of their preparation
ATE556149T1 (en) * 1999-02-23 2012-05-15 Caliper Life Sciences Inc MANIPULATION OF MICROPARTICLES IN MICROFLUIDIC SYSTEMS
US6309836B1 (en) * 1999-10-05 2001-10-30 Marek Kwiatkowski Compounds for protecting hydroxyls and methods for their use
US7057026B2 (en) * 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
DE10214395A1 (en) * 2002-03-30 2003-10-23 Dmitri Tcherkassov Parallel sequencing of nucleic acid segments, useful for detecting single-nucleotide polymorphisms, by single-base extensions with labeled nucleotide

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5852182A (en) * 1990-01-11 1998-12-22 Isis Pharmaceuticals Inc. Thiol-derivatized oligonucleosides
US6265588B1 (en) * 1997-01-28 2001-07-24 Aventis Pharma Deutschland Gmbh Isoxazole and crotonamide derivatives and their use as pharmaceuticals and diagnostics

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP1879906A4 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9476054B2 (en) 2005-06-15 2016-10-25 Complete Genomics, Inc. Two-adaptor library for high-throughput sequencing on DNA arrays
US9228228B2 (en) 2006-10-27 2016-01-05 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
US9334490B2 (en) 2006-11-09 2016-05-10 Complete Genomics, Inc. Methods and compositions for large-scale analysis of nucleic acids using DNA deletions
US9146248B2 (en) 2013-03-14 2015-09-29 Intelligent Bio-Systems, Inc. Apparatus and methods for purging flow cells in nucleic acid sequencing instruments
US9591268B2 (en) 2013-03-15 2017-03-07 Qiagen Waltham, Inc. Flow cell alignment methods and systems

Also Published As

Publication number Publication date
JP2008526877A (en) 2008-07-24
WO2006074351A2 (en) 2006-07-13
EP1879906A4 (en) 2009-07-22
EP1879906A2 (en) 2008-01-23

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