WO2005118882A3 - Verfahren zur taxonspezifischen zellidentifizierung und zellsortierung auf grampositive bakterien und vorrichtungen hierfür - Google Patents

Verfahren zur taxonspezifischen zellidentifizierung und zellsortierung auf grampositive bakterien und vorrichtungen hierfür Download PDF

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Publication number
WO2005118882A3
WO2005118882A3 PCT/EP2005/005408 EP2005005408W WO2005118882A3 WO 2005118882 A3 WO2005118882 A3 WO 2005118882A3 EP 2005005408 W EP2005005408 W EP 2005005408W WO 2005118882 A3 WO2005118882 A3 WO 2005118882A3
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WO
WIPO (PCT)
Prior art keywords
gram
positive bacteria
taxon
cells
specific identification
Prior art date
Application number
PCT/EP2005/005408
Other languages
English (en)
French (fr)
Other versions
WO2005118882A2 (de
Inventor
Bertrand Tankouo Sandjong
Original Assignee
Eppendorf Ag
Bertrand Tankouo Sandjong
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eppendorf Ag, Bertrand Tankouo Sandjong filed Critical Eppendorf Ag
Priority to EP05750160A priority Critical patent/EP1749107A2/de
Publication of WO2005118882A2 publication Critical patent/WO2005118882A2/de
Publication of WO2005118882A3 publication Critical patent/WO2005118882A3/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Die vorliegende Erfindung betrifft ein Verfahren zur taxonspezifischen Zellidentifizierung und/oder Zellsortierung auf grampositive Bakterien. Darüber hinaus stellt die vorliegende Erfindung einen Kit zum Identifizieren und Sortieren von grampositiven Bakterien und Vorrichtungen zur taxonspezifischen Zellidentifizierung und/oder Zellsortierung auf grampositive Bakterien, beispielsweise Mikrotiterplatten und Objektträger, bereit.
PCT/EP2005/005408 2004-05-26 2005-05-18 Verfahren zur taxonspezifischen zellidentifizierung und zellsortierung auf grampositive bakterien und vorrichtungen hierfür WO2005118882A2 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP05750160A EP1749107A2 (de) 2004-05-26 2005-05-18 Verfahren zur taxonspezifischen zellidentifizierung und zellsortierung auf grampositive bakterien und vorrichtungen hierfür

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE200410025710 DE102004025710A1 (de) 2004-05-26 2004-05-26 Verfahren zur taxonspezifischen Zellidentifizierung und Zellsortierung auf grampositive Bakterien und Vorrichtungen hierfür
DE102004025710.8 2004-05-26

Publications (2)

Publication Number Publication Date
WO2005118882A2 WO2005118882A2 (de) 2005-12-15
WO2005118882A3 true WO2005118882A3 (de) 2006-02-02

Family

ID=35276684

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2005/005408 WO2005118882A2 (de) 2004-05-26 2005-05-18 Verfahren zur taxonspezifischen zellidentifizierung und zellsortierung auf grampositive bakterien und vorrichtungen hierfür

Country Status (3)

Country Link
EP (1) EP1749107A2 (de)
DE (1) DE102004025710A1 (de)
WO (1) WO2005118882A2 (de)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113528684A (zh) * 2021-08-04 2021-10-22 领航基因科技（杭州）有限公司 用于鉴别革兰氏阴性菌和革兰氏阳性菌的数字pcr检测试剂盒和应用

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0497464A1 (de) * 1991-01-31 1992-08-05 Amoco Corporation Schnellnachweis von Mikroben durch in situ-Hybridisation in wässeriger Suspension
WO1999018234A2 (en) * 1997-10-08 1999-04-15 Suez Lyonnaise Des Eaux Means for qualitative and quantitative analysis of microbial populations potentially present in a sample
WO1999054502A1 (en) * 1998-04-20 1999-10-28 Academisch Ziekenhuis Groningen Method for the rapid determination of bacteria
EP1096024A1 (de) * 1999-10-28 2001-05-02 Remacle, José Verfahren und Reagentiensatz zum Auswählen und/oder Quantifizieren von multiplen homologen Nukleinsäuresequenzen unter Verwendung von Sondenanordnungen
US20030175709A1 (en) * 2001-12-20 2003-09-18 Murphy George L. Method and system for depleting rRNA populations
WO2003106676A1 (ja) * 2002-06-14 2003-12-24 日立ソフトウエアエンジニアリング株式会社 微生物同定用プローブ及びそれを用いた同定方法
US20040010129A1 (en) * 2001-11-01 2004-01-15 Po-Hsiang Hsu Nucleic acid kit for bacterial pathogen diagnosis and method for using the same
WO2004033720A2 (en) * 2002-10-09 2004-04-22 Hôpitaux Universitaires De Geneve Analytical chip for detection of 16s-rrna from clinically relevant bacteria and analytical method based thereon

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988003957A1 (en) * 1986-11-24 1988-06-02 Gen-Probe Incorporated Nucleic acid probes for detection and/or quantitation of non-viral organisms
DE19955303A1 (de) * 1999-11-17 2001-05-31 Creatogen Ag Test von Mikroorganismen
DE10214153A1 (de) * 2002-03-28 2003-11-13 Vermicon Ag Verfahren zum Nachweis von Mikroorganismen mittels in situ-Hybridisierung und Durchflusszytometrie
JP2006500010A (ja) * 2002-07-18 2006-01-05 ヘンケル・コマンディットゲゼルシャフト・アウフ・アクチエン 微生物検出用オリゴヌクレオチド

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0497464A1 (de) * 1991-01-31 1992-08-05 Amoco Corporation Schnellnachweis von Mikroben durch in situ-Hybridisation in wässeriger Suspension
WO1999018234A2 (en) * 1997-10-08 1999-04-15 Suez Lyonnaise Des Eaux Means for qualitative and quantitative analysis of microbial populations potentially present in a sample
WO1999054502A1 (en) * 1998-04-20 1999-10-28 Academisch Ziekenhuis Groningen Method for the rapid determination of bacteria
EP1096024A1 (de) * 1999-10-28 2001-05-02 Remacle, José Verfahren und Reagentiensatz zum Auswählen und/oder Quantifizieren von multiplen homologen Nukleinsäuresequenzen unter Verwendung von Sondenanordnungen
US20040010129A1 (en) * 2001-11-01 2004-01-15 Po-Hsiang Hsu Nucleic acid kit for bacterial pathogen diagnosis and method for using the same
US20030175709A1 (en) * 2001-12-20 2003-09-18 Murphy George L. Method and system for depleting rRNA populations
WO2003106676A1 (ja) * 2002-06-14 2003-12-24 日立ソフトウエアエンジニアリング株式会社 微生物同定用プローブ及びそれを用いた同定方法
WO2004033720A2 (en) * 2002-10-09 2004-04-22 Hôpitaux Universitaires De Geneve Analytical chip for detection of 16s-rrna from clinically relevant bacteria and analytical method based thereon

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* Cited by examiner, † Cited by third party
Title
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DATABASE EMBL [online] 18 August 2003 (2003-08-18), "Sequence 5041 from patent US 6593114.", XP002354848, retrieved from EBI accession no. EM_PRO:AR358923 Database accession no. AR358923 *
DATABASE EMBL [online] 18 February 2001 (2001-02-18), "1M0567P11F Mouse 10kb plasmid UUGC1M library Mus musculus genomic clone UUGC1M0567P11 F, DNA sequence.", XP002354849, retrieved from EBI accession no. EM_PRO:AZ767749 Database accession no. AZ767749 *
LUDWIG W ET AL: "PCR-BASED PREPARATION OF 23S RRNA-TARGETED GROUP-SPECIFIC POLYNUCLEOTIDE PROBES", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, WASHINGTON,DC, US, vol. 60, no. 9, September 1994 (1994-09-01), pages 3236 - 3244, XP000921283, ISSN: 0099-2240 *
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THOMAS J-C ET AL: "QUANTITATIVE FLOW CYTOMETRIC DETECTION OF SPECIFIC MICROORGANISMS IN SOIL SAMPLES USING RRNA TARGETED FLUORESCENT PROBES AND ETHIDIUM BROMIDE", CYTOMETRY, ALAN LISS, NEW YORK, US, vol. 27, no. 3, 1997, pages 224 - 232, XP009014220, ISSN: 0196-4763 *

Also Published As

Publication number Publication date
DE102004025710A1 (de) 2005-12-22
EP1749107A2 (de) 2007-02-07
WO2005118882A2 (de) 2005-12-15

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