WO2005114169A1 - Parallel processing microfluid chip - Google Patents

Parallel processing microfluid chip Download PDF

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Publication number
WO2005114169A1
WO2005114169A1 PCT/EP2004/050893 EP2004050893W WO2005114169A1 WO 2005114169 A1 WO2005114169 A1 WO 2005114169A1 EP 2004050893 W EP2004050893 W EP 2004050893W WO 2005114169 A1 WO2005114169 A1 WO 2005114169A1
Authority
WO
WIPO (PCT)
Prior art keywords
microfluidic chip
coupled
microfluidic
column
port
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2004/050893
Other languages
English (en)
French (fr)
Inventor
Bernd Glatz
Herbert Anderer
Martin Bäuerle
Karsten Kraiczek
Jan Eickhoff
Patrick Müller
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Agilent Technologies Inc
Original Assignee
Agilent Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agilent Technologies Inc filed Critical Agilent Technologies Inc
Priority to EP04741632A priority Critical patent/EP1751538B1/en
Priority to PCT/EP2004/050893 priority patent/WO2005114169A1/en
Priority to DE602004024249T priority patent/DE602004024249D1/de
Publication of WO2005114169A1 publication Critical patent/WO2005114169A1/en
Priority to US11/603,404 priority patent/US20070071638A1/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G01N30/466—Flow patterns using more than one column with separation columns in parallel
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/16—Injection
    • G01N30/20—Injection using a sampling valve
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6095—Micromachined or nanomachined, e.g. micro- or nanosize
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J41/00—Discharge tubes for measuring pressure of introduced gas or for detecting presence of gas; Discharge tubes for evacuation by diffusion of ions
    • H01J41/02—Discharge tubes for measuring pressure of introduced gas or for detecting presence of gas
    • H01J41/04—Discharge tubes for measuring pressure of introduced gas or for detecting presence of gas with ionisation by means of thermionic cathodes
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/02—Details
    • H01J49/04—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0622—Valves, specific forms thereof distribution valves, valves having multiple inlets and/or outlets, e.g. metering valves, multi-way valves
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/06—Preparation
    • G01N30/08—Preparation using an enricher
    • G01N2030/085—Preparation using an enricher using absorbing precolumn
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/16—Injection
    • G01N30/20—Injection using a sampling valve
    • G01N2030/201—Injection using a sampling valve multiport valves, i.e. having more than two ports
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/16—Injection
    • G01N30/20—Injection using a sampling valve
    • G01N2030/202—Injection using a sampling valve rotary valves
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/72—Mass spectrometers
    • G01N30/7233—Mass spectrometers interfaced to liquid or supercritical fluid chromatograph
    • G01N30/724—Nebulising, aerosol formation or ionisation

Definitions

  • the microfluidic chip comprises at least one port, in particular an array of ports, which is adapted to interact with a multi-route switching valve, in particular for sealing and/or opening and/or connecting the port, in particular the array of ports, of the microfluidic chip.
  • the different processes can be controlled, switched, and time-shifted by the valve.
  • FIG. 1 shows a hydraulic layout of a microfluidic device
  • the multi-route switching device 17 realizes two different settings in a first setting - symbolized with continuous lines - a first switching port 19 is connected to a second outlet port 25 and a second switching port 21 is connected to a first outlet port 23 of the microfluidic device 3.
  • Figure 2 shows the same hydraulic functionality as described in Figure 1 realized by the microfluidic chip 1. Therefore, only the differences are described .
  • the multi-route switching devices 17 and 65 comprise fluid conducting grooves 90 and parallel flow paths 82 and 84 for coupling the ports of the microfluidic device 63 as follows:
  • the first combination column 5 can be reloaded with a new sample concurrently.
  • the ten-port multi-route switching valve 99 is adapted for coupling and/or sealing fourteen ports 75, 81 , 83, 85, 86, 109, 111 , 113, 115, 117, and 119 of the microfluidic chips 93:
  • the multi-route switching valves 97 and 99 comprise fluid conducting grooves 90 and parallel flow paths 82 for coupling the ports of the microfluidic device 93 as follows:
  • the first enrichment column 101 is coupled upstream to the sample inlet port 75 via the first enrichment column inlet port 109 and via the ten-port multi-route switching valve 99 and downstream to the second outlet port 85 via the first enrichment column outlet port 113 and via the ten-port multi-route switching valve 99.
  • the sample inlet port 75 can be coupled to the sample-feeding device 13.
  • the second outlet port 85 can be coupled to the waste container 29.
  • a bar 61 symbolizes the different settings "A" to "D" of the ten-port multi- route switching valve 99 and of the four-port multi-route switching valve 97 with capital letters A to D written in sectors of the bar 61.
  • the length of the time axis of each sector represents the time span for the indicated setting.
  • the valves are switched concurrently.
  • Optional settings are indicated with two capital letters separated by a slash.
  • the microfluidic device 125 is adapted to be coupled with just one sample-feeding device 13 - symbolized by the arrow 9 - and to be coupled with just one microfluidic feeding device - symbolized by the arrow 71 - in this embodiment with one nano-pump 67.
  • the inlet ports 212 and 214 can be coupled at the bottom side of the microfluidic chip 201 to two nano-pumps. Inflowing liquid can flow from the bottom side of the microfluidic chip 201 through the holes through the parallel flow paths 259 and 261 of the middle layer 255 of the microfluidic chip 201 to the outlet ports 217 and 219 and to the parallel ports 216 and 218 to the top side of the microfluidic chip 201.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Nanotechnology (AREA)
  • Dispersion Chemistry (AREA)
  • Sampling And Sample Adjustment (AREA)
PCT/EP2004/050893 2004-05-22 2004-05-22 Parallel processing microfluid chip Ceased WO2005114169A1 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP04741632A EP1751538B1 (en) 2004-05-22 2004-05-22 Parallel processing microfluid chip
PCT/EP2004/050893 WO2005114169A1 (en) 2004-05-22 2004-05-22 Parallel processing microfluid chip
DE602004024249T DE602004024249D1 (https=) 2004-05-22 2004-05-22
US11/603,404 US20070071638A1 (en) 2004-05-22 2006-11-22 Parallel processing microfluidic chip

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/EP2004/050893 WO2005114169A1 (en) 2004-05-22 2004-05-22 Parallel processing microfluid chip

Publications (1)

Publication Number Publication Date
WO2005114169A1 true WO2005114169A1 (en) 2005-12-01

Family

ID=34957817

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2004/050893 Ceased WO2005114169A1 (en) 2004-05-22 2004-05-22 Parallel processing microfluid chip

Country Status (4)

Country Link
US (1) US20070071638A1 (https=)
EP (1) EP1751538B1 (https=)
DE (1) DE602004024249D1 (https=)
WO (1) WO2005114169A1 (https=)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009003520A1 (en) * 2007-07-04 2009-01-08 Agilent Technologies, Inc. Two-valve arrangement for liquid chromatography
WO2007084425A3 (en) * 2006-01-19 2009-04-02 Kionix Inc Flexible and modular microfluidic device
EP2065703A1 (en) * 2007-11-30 2009-06-03 Services Pétroliers Schlumberger Natural gas analyzer on a micro-chip
US7608160B2 (en) 2004-10-13 2009-10-27 Rheonix, Inc. Laminated microfluidic structures and method for making
US7837821B2 (en) 2004-10-13 2010-11-23 Rheonix, Inc. Laminated microfluidic structures and method for making
US8293053B2 (en) 2004-10-13 2012-10-23 Rheonix, Inc. Fabrication methods for microfluidic pump and valve structures
CN102896010A (zh) * 2012-10-26 2013-01-30 中国科学技术大学 一种微流控分离芯片、分离器及超滤装置
EP2285462B1 (en) * 2008-05-27 2020-12-16 PerkinElmer Health Sciences, Inc. Method and device for modulating flow of a fluid in a chromatography system

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7811452B2 (en) * 2008-01-30 2010-10-12 Agilent Technologies, Inc. Microfluidic device for sample analysis
CN103897985B (zh) * 2012-12-25 2016-08-10 中国科学院青岛生物能源与过程研究所 活体单细胞分选电子控制系统
CN209194072U (zh) 2015-06-30 2019-08-02 珀金埃尔默健康科学股份有限公司 气相色谱系统
EP3423821B1 (en) 2016-04-04 2023-06-21 Entech Instruments Inc. Multi-capillary column pre-concentration system for enhanced sensitivity in gas chromatography (gc) and gas chromatography-mass spectrometry (gcms)
JP7072134B2 (ja) 2017-10-25 2022-05-20 エンテック インスツルメンツ インコーポレイテッド ガスクロマトグラフィで使用するための試料予備濃縮システム及び方法
US11162925B2 (en) 2017-11-03 2021-11-02 Entech Instruments Inc. High performance sub-ambient temperature multi-capillary column preconcentration system for volatile chemical analysis by gas chromatography
US12360088B2 (en) * 2020-02-21 2025-07-15 Shimadzu Corporation Liquid chromatograph
US11946912B2 (en) 2020-06-30 2024-04-02 Entech Instruments Inc. System and method of trace-level analysis of chemical compounds

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1162464A1 (en) * 2000-06-08 2001-12-12 Pfizer Products Inc. Method and device for increasing efficiency of bioanalysis
WO2004015411A1 (en) * 2002-08-08 2004-02-19 Nanostream, Inc. Systems and methods for high-throughput microfluidic sample analysis
WO2004042383A1 (en) * 2002-10-31 2004-05-21 Nanostream, Inc. System and method for performing multiple parallel chromatographic separations

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6635173B2 (en) * 2000-12-28 2003-10-21 Cohesive Technologies, Inc. Multi column chromatography system

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1162464A1 (en) * 2000-06-08 2001-12-12 Pfizer Products Inc. Method and device for increasing efficiency of bioanalysis
WO2004015411A1 (en) * 2002-08-08 2004-02-19 Nanostream, Inc. Systems and methods for high-throughput microfluidic sample analysis
WO2004042383A1 (en) * 2002-10-31 2004-05-21 Nanostream, Inc. System and method for performing multiple parallel chromatographic separations

Cited By (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8293053B2 (en) 2004-10-13 2012-10-23 Rheonix, Inc. Fabrication methods for microfluidic pump and valve structures
US7608160B2 (en) 2004-10-13 2009-10-27 Rheonix, Inc. Laminated microfluidic structures and method for making
US8535020B2 (en) 2004-10-13 2013-09-17 Rheonix, Inc. Microfluidic pump and valve structures and fabrication methods
US7837821B2 (en) 2004-10-13 2010-11-23 Rheonix, Inc. Laminated microfluidic structures and method for making
US7976795B2 (en) 2006-01-19 2011-07-12 Rheonix, Inc. Microfluidic systems
WO2007084425A3 (en) * 2006-01-19 2009-04-02 Kionix Inc Flexible and modular microfluidic device
JP2009524054A (ja) * 2006-01-19 2009-06-25 キオニックス インコーポレイテッド 微小流体チップ及び分析システム
US7959875B2 (en) 2006-01-19 2011-06-14 Rheonix, Inc. Microfluidic chips and assay systems
US8257586B2 (en) 2007-07-04 2012-09-04 Agilent Technologies, Inc. Two-valve arrangement for liquid chromatography
CN101730844A (zh) * 2007-07-04 2010-06-09 安捷伦科技有限公司 用于液相色谱的两阀结构
WO2009003520A1 (en) * 2007-07-04 2009-01-08 Agilent Technologies, Inc. Two-valve arrangement for liquid chromatography
CN101730844B (zh) * 2007-07-04 2013-05-22 安捷伦科技有限公司 用于液相色谱的两阀结构
GB2467672A (en) * 2007-11-30 2010-08-11 Schlumberger Holdings Natural gas analyzer on a micro-chip
WO2009068201A1 (en) * 2007-11-30 2009-06-04 Services Petroliers Schlumberger Natural gas analyzer on a micro-chip
EP2065703A1 (en) * 2007-11-30 2009-06-03 Services Pétroliers Schlumberger Natural gas analyzer on a micro-chip
US8621912B2 (en) 2007-11-30 2014-01-07 Schlumberger Technology Corporation Natural gas analyzer on a micro-chip
EP2285462B1 (en) * 2008-05-27 2020-12-16 PerkinElmer Health Sciences, Inc. Method and device for modulating flow of a fluid in a chromatography system
CN102896010A (zh) * 2012-10-26 2013-01-30 中国科学技术大学 一种微流控分离芯片、分离器及超滤装置
CN102896010B (zh) * 2012-10-26 2014-06-18 中国科学技术大学 一种微流控分离芯片、分离器及超滤装置

Also Published As

Publication number Publication date
EP1751538B1 (en) 2009-11-18
DE602004024249D1 (https=) 2009-12-31
US20070071638A1 (en) 2007-03-29
EP1751538A1 (en) 2007-02-14

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