WO2005113743A3 - Vorrichtung zur durchführung einer liquid-air-kultur von epithel - Google Patents

Vorrichtung zur durchführung einer liquid-air-kultur von epithel Download PDF

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Publication number
WO2005113743A3
WO2005113743A3 PCT/EP2005/005446 EP2005005446W WO2005113743A3 WO 2005113743 A3 WO2005113743 A3 WO 2005113743A3 EP 2005005446 W EP2005005446 W EP 2005005446W WO 2005113743 A3 WO2005113743 A3 WO 2005113743A3
Authority
WO
WIPO (PCT)
Prior art keywords
epithelium
liquid
carrying
air culture
culture
Prior art date
Application number
PCT/EP2005/005446
Other languages
English (en)
French (fr)
Other versions
WO2005113743A2 (de
Inventor
Juergen Ostwald
Original Assignee
Univ Rostock
Juergen Ostwald
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Rostock, Juergen Ostwald filed Critical Univ Rostock
Publication of WO2005113743A2 publication Critical patent/WO2005113743A2/de
Publication of WO2005113743A3 publication Critical patent/WO2005113743A3/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0629—Keratinocytes; Whole skin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/14—Scaffolds; Matrices
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C12N2533/40—Polyhydroxyacids, e.g. polymers of glycolic or lactic acid (PGA, PLA, PLGA); Bioresorbable polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Dermatology (AREA)
  • Immunology (AREA)
  • Sustainable Development (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Die vorliegende Erfindung betrifft eine Vorrichtung und ein Kit zur Durchführung einer Liquid-Air-Kultur von Epithel. Wei­terhin betrifft die Erfindung ein Verfahren zur Herstellung einer mit Kollagen beschichteten Matrix, die Teil der Vorrich­tung ist, die Verwendung der genannten Vorrichtung zur Liquid­-Air-Kultur von Epithel und ein Verfahren zur Liquid-Air-Kultur von Epithel.
PCT/EP2005/005446 2004-05-19 2005-05-19 Vorrichtung zur durchführung einer liquid-air-kultur von epithel WO2005113743A2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102004024834.6 2004-05-19
DE102004024834A DE102004024834A1 (de) 2004-05-19 2004-05-19 Vorrichtung zur Durchführung einer Liquid-Air-Kultur von Epithel

Publications (2)

Publication Number Publication Date
WO2005113743A2 WO2005113743A2 (de) 2005-12-01
WO2005113743A3 true WO2005113743A3 (de) 2006-06-15

Family

ID=34975230

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2005/005446 WO2005113743A2 (de) 2004-05-19 2005-05-19 Vorrichtung zur durchführung einer liquid-air-kultur von epithel

Country Status (2)

Country Link
DE (1) DE102004024834A1 (de)
WO (1) WO2005113743A2 (de)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE602008002545D1 (de) * 2008-02-01 2010-10-28 Eppendorf Ag Kulturplatte mit Klappe zur seitlichen Belüftung
DE102010064098B4 (de) * 2010-12-23 2014-02-13 Hochschule Wismar Zellreaktor
DE202016000904U1 (de) 2016-02-11 2016-06-06 Frankenförder Forschungsgesellschaft mbH für Betriebswirtschaft, Ernährung und ökologischen Landbau Zelllinie aus Gewebe von Pferden und deren Anwendung

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4608342A (en) * 1983-05-13 1986-08-26 Stephan Nees Method of growing a confluent layer of cells on a porous or semi-permeable substrate and apparatus for practicing the method
DE3923279A1 (de) * 1989-07-14 1990-01-18 Will W Prof Dr Minuth Minusheets ist ein neues produkt, um zellen in beliebigen behaeltnissen in hochdifferenzierter form auf einer moeglichst natuerlichen unterlage zu kultivieren
DE3938632C1 (en) * 1989-11-21 1991-03-14 Adelbert Prof. Dr.Dr. 8046 Garching De Bacher Culturing living animal cells - using natural or synthetic polymer substrate vacuum deposition coated with e.g. nitride of Gp=III element
US5122470A (en) * 1988-07-05 1992-06-16 Banes Albert J Floating cell culture device and method
US5175092A (en) * 1989-05-04 1992-12-29 Millipore Corporation Vitro toxicology kit and method therefor

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3050879B2 (ja) * 1987-04-28 2000-06-12 ザ・リージエント・オブ・ザ・ユニバーシテイ・オブ・カリフオルニア 複合皮膚代用品の調製方法及び装置
JPH0779770A (ja) * 1993-09-17 1995-03-28 Kobe Steel Ltd 生物細胞の培養方法及び培養装置
JP3543869B2 (ja) * 1995-03-07 2004-07-21 株式会社メニコン 培養皮膚およびその製造法
IN183604B (de) * 1995-05-16 2000-03-04 Indian Inst Technology
DE10201259A1 (de) * 2002-01-15 2003-08-28 Augustinus Bader Vorrichtung zum Züchten oder Kultivieren von Zellen in einem dosenartigen Behälter

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4608342A (en) * 1983-05-13 1986-08-26 Stephan Nees Method of growing a confluent layer of cells on a porous or semi-permeable substrate and apparatus for practicing the method
US5122470A (en) * 1988-07-05 1992-06-16 Banes Albert J Floating cell culture device and method
US5175092A (en) * 1989-05-04 1992-12-29 Millipore Corporation Vitro toxicology kit and method therefor
DE3923279A1 (de) * 1989-07-14 1990-01-18 Will W Prof Dr Minuth Minusheets ist ein neues produkt, um zellen in beliebigen behaeltnissen in hochdifferenzierter form auf einer moeglichst natuerlichen unterlage zu kultivieren
DE3938632C1 (en) * 1989-11-21 1991-03-14 Adelbert Prof. Dr.Dr. 8046 Garching De Bacher Culturing living animal cells - using natural or synthetic polymer substrate vacuum deposition coated with e.g. nitride of Gp=III element

Also Published As

Publication number Publication date
WO2005113743A2 (de) 2005-12-01
DE102004024834A1 (de) 2006-01-12

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