WO2005104649A3 - Levaduras (bgs4delta) autoliticas, procedimiento de obtención y sus aplicaciones en procedimientos de expresión de proteínas - Google Patents

Levaduras (bgs4delta) autoliticas, procedimiento de obtención y sus aplicaciones en procedimientos de expresión de proteínas Download PDF

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Publication number
WO2005104649A3
WO2005104649A3 PCT/ES2005/070050 ES2005070050W WO2005104649A3 WO 2005104649 A3 WO2005104649 A3 WO 2005104649A3 ES 2005070050 W ES2005070050 W ES 2005070050W WO 2005104649 A3 WO2005104649 A3 WO 2005104649A3
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WIPO (PCT)
Prior art keywords
yeasts
applications
bgs4
autolytic
delta
Prior art date
Application number
PCT/ES2005/070050
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English (en)
French (fr)
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WO2005104649A2 (es
Inventor
Elcorobarrutia Juan Carl Ribas
Bravo Angel Duran
Cortes Juan Carlos Garcia
Original Assignee
Consejo Superior Investigacion
Elcorobarrutia Juan Carl Ribas
Bravo Angel Duran
Cortes Juan Carlos Garcia
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Consejo Superior Investigacion, Elcorobarrutia Juan Carl Ribas, Bravo Angel Duran, Cortes Juan Carlos Garcia filed Critical Consejo Superior Investigacion
Publication of WO2005104649A2 publication Critical patent/WO2005104649A2/es
Publication of WO2005104649A3 publication Critical patent/WO2005104649A3/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Mycology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La invención describe unas nuevas levaduras eficaces para la expresión y obtención de proteínas heterólogas de interés comercial que permite su secreción al medio mediante autolisis regulada. La ventaja es que se produce una lisis natural, sin producir otro tipo de alteraciones celulares como ocurre en otros procedimientos y que la rotura celular es inducida, por lo que se evita cualquier método de tratamiento físico, de otra manera necesario para romper la pared celular de la levadura y liberar su contenido. Igualmente, se describe su método de obtención y sus aplicaciones.
PCT/ES2005/070050 2004-04-30 2005-04-25 Levaduras (bgs4delta) autoliticas, procedimiento de obtención y sus aplicaciones en procedimientos de expresión de proteínas WO2005104649A2 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ES200401042A ES2274664B1 (es) 2004-04-30 2004-04-30 Levaduras bgs4delta autoliticas, procedimiento de obtencion y sus aplicaciones en procedimientos de expresion de proteinas.
ESP200401042 2004-04-30

Publications (2)

Publication Number Publication Date
WO2005104649A2 WO2005104649A2 (es) 2005-11-10
WO2005104649A3 true WO2005104649A3 (es) 2006-05-11

Family

ID=35242089

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/ES2005/070050 WO2005104649A2 (es) 2004-04-30 2005-04-25 Levaduras (bgs4delta) autoliticas, procedimiento de obtención y sus aplicaciones en procedimientos de expresión de proteínas

Country Status (2)

Country Link
ES (1) ES2274664B1 (es)
WO (1) WO2005104649A2 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK3327112T3 (en) 2012-07-19 2020-05-04 Dsm Ip Assets Bv Agse-deficient stamme

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996002629A2 (es) * 1994-07-14 1996-02-01 Universidad Complutense De Madrid Procedimiento de liberacion de proteinas heterologas de cepas de saccharomyces cerevisiae

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996002629A2 (es) * 1994-07-14 1996-02-01 Universidad Complutense De Madrid Procedimiento de liberacion de proteinas heterologas de cepas de saccharomyces cerevisiae

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
CORTES J.C. ET AL: "Localization of the (1,3)beta-D-glucan synthase catalytic subunit homologue Bgs1p/Cps1p from fission yeast suggests that it is involved in septation, polarized growth, mating, spore wall formation and spore germination", J. CELL SCI., vol. 115, no. 21, 2002, pages 4081 - 4096 *
CORTES J.C. ET AL: "The novel fission yeast (1,3)beta-D-glucan synthase catalytic subunit Bgs4p is essential during both cytokinesis and polarized growth", J. CELL SCI., vol. 118, no. 1, 2005, pages 157 - 174 *
DIJKGRAAF G.J. ET AL: "Mutations in Fks1p affect the cell wall content of beta-1,3- and beta-1,6-glucan in Saccharomyces cerevisiae", YEAST, vol. 19, no. 8, 2002, pages 671 - 690 *
DOUGLAS C.M.: "Fungal beta(1,3)-D-glucan synthesis", MED. MYCOL., vol. 39, no. 1, 2001, pages 55 - 66 *
MARTIN V. ET AL: "bgs2+, a sporulation-specific glucan synthase homologue is required for proper ascospore wall maturation in fission yeast", MOL. MICROBIOL., vol. 38, no. 2, 2000, pages 308 - 321 *
MARTIN V. ET AL: "Bgs3p, a putative 1,3-beta-glucan synthase subunit, is required for cell wall assembly in Schizosaccharomyces pombe", EUKARYOT. CELL., vol. 2, no. 1, 2003, pages 159 - 169 *
RIBAS J.C. ET AL: "Isolation and characterization of Schizosaccharomyces pombe mutants defective in cell wall (1-3)beta-D-glucan", J. BACTERIOL., vol. 173, no. 11, 1991, pages 3456 - 3462, XP002504461 *
TERASHIMA H. ET AL: "Up-regulation of genes encoding glycosylphosphatidylinositol (GPI)-attached proteins in response to cell wall damage caused by disruption of FKS1 in Saccharomyces cerevisiae", MOL. GEN. GENET., vol. 264, no. 1-2, 2000, pages 64 - 74, XP002444392, DOI: doi:10.1007/s004380000285 *
ZHANG N. ET AL: "Genetically controlled cell lysis in the yeast Saccharomyces cerevisiae", BIOTECHNOL. BIOENG., vol. 64, no. 5, 1999, pages 607 - 615, XP000852988, DOI: doi:10.1002/(SICI)1097-0290(19990905)64:5<607::AID-BIT11>3.0.CO;2-0 *

Also Published As

Publication number Publication date
ES2274664A1 (es) 2007-05-16
WO2005104649A2 (es) 2005-11-10
ES2274664B1 (es) 2008-04-01

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