WO2005095588A1 - Écorce glutamatergique précurseur de neurone produisant une écorce glutamatergique précurseuse de neurone seule in vivo. - Google Patents

Écorce glutamatergique précurseur de neurone produisant une écorce glutamatergique précurseuse de neurone seule in vivo. Download PDF

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Publication number
WO2005095588A1
WO2005095588A1 PCT/JP2005/006821 JP2005006821W WO2005095588A1 WO 2005095588 A1 WO2005095588 A1 WO 2005095588A1 JP 2005006821 W JP2005006821 W JP 2005006821W WO 2005095588 A1 WO2005095588 A1 WO 2005095588A1
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WO
WIPO (PCT)
Prior art keywords
cells
cerebral
cortex
cerebral cortical
cell
Prior art date
Application number
PCT/JP2005/006821
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English (en)
Japanese (ja)
Inventor
Nobuaki Tamamaki
Original Assignee
Kyoto University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Kyoto University filed Critical Kyoto University
Publication of WO2005095588A1 publication Critical patent/WO2005095588A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0619—Neurons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells

Abstract

Une méthode comprenant:l’étape de préparation d’une zone cellulaire produite à partir d’une écorce de tissu, de souches de cellules embryonnaires ou de souches de cellules nerveuses ; l’étape de transfère un vecteur d'expression, qui à un prometteur sélectionné à partir de NEX(Math2) NeuroD, lié-à-un-facteur, Neurogin 1, Neurogen 2, Svet 1, Otx1 et Tbr2 et un gène pour constater l’expression de cette molécule attachée en aval du prometteur en cellules individuelles dans la souche cellulaire ; l’étape d’isolement des écorces de neurones glutamatergiques et des écorces glutamatergiques précurseuses en utilisant la fonction de l’expression de la fonction du vecteur d'expression comme indicateur; et ; l’étape d’isolement des écorces précurseuses de neurones glutamatergiques en utilisant la capacité de prolifération comme indicateur. Selon cette méthode, il est possible d’obtenir une écorce précurseuse de neurones glutamatergiques pour le traitement de dysfonctionnement causé par une blessure d’écorce, Etc.
PCT/JP2005/006821 2004-03-31 2005-03-31 Écorce glutamatergique précurseur de neurone produisant une écorce glutamatergique précurseuse de neurone seule in vivo. WO2005095588A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2004105645 2004-03-31
JP2004/105645 2004-03-31

Publications (1)

Publication Number Publication Date
WO2005095588A1 true WO2005095588A1 (fr) 2005-10-13

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PCT/JP2005/006821 WO2005095588A1 (fr) 2004-03-31 2005-03-31 Écorce glutamatergique précurseur de neurone produisant une écorce glutamatergique précurseuse de neurone seule in vivo.

Country Status (1)

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WO (1) WO2005095588A1 (fr)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002064748A2 (fr) * 2001-02-14 2002-08-22 Furcht Leo T Cellules souches adultes totipotentes, sources de ces cellules, procedes d'obtention et de maintien de ces dernieres, procedes de differentiation de ces cellules, procedes d'utilisation correspondants et cellules derivees des cellules susmentionnees

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002064748A2 (fr) * 2001-02-14 2002-08-22 Furcht Leo T Cellules souches adultes totipotentes, sources de ces cellules, procedes d'obtention et de maintien de ces dernieres, procedes de differentiation de ces cellules, procedes d'utilisation correspondants et cellules derivees des cellules susmentionnees

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BARTHOLOMA A. ET AL: "NEX-1: a novel brain specific helix-loop-helix protein with autoregulation and sustained expression in mature cortical neurons.", MECHANISM OF DEVELOPMENT., vol. 48, no. 3, 1994, pages 217 - 228, XP002992002 *
SCHWAB M.H. ET AL: "Neuronal basic helix-loop-helix proteins (NEX and BETA2/Neuro D) regulate terminal granule cell differentiation in the hippocampus.", THE JOURNAL OF NEUROSCIENCE., vol. 20, 2000, pages 3714 - 3724, XP002991783 *
SCHWAB M.H. ET AL: "Neuronal basic helix-loop-helix proteins (NEX, neuroD, NDRF): Spatiotemporal expression and targeted disruption of the NEX gene in transgenic mice.", THE JOURNAL OF NEUROSCIENCE., vol. 18, 1998, pages 1408 - 1418, XP002992032 *
WU S.X. ET AL: "Pyramidal neuron production in the extraventricular zone of the mouse neocortex.", SOCIETY FOR NEUROSCIENCE ABSTRACT VIEWER AND ITINERARY PLANNER., vol. 243, no. 6, 2003 *

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