WO2005094353A3 - Techniques de production de lymphocytes t regulateurs et utilisation de ceux-ci - Google Patents

Techniques de production de lymphocytes t regulateurs et utilisation de ceux-ci Download PDF

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Publication number
WO2005094353A3
WO2005094353A3 PCT/US2005/010597 US2005010597W WO2005094353A3 WO 2005094353 A3 WO2005094353 A3 WO 2005094353A3 US 2005010597 W US2005010597 W US 2005010597W WO 2005094353 A3 WO2005094353 A3 WO 2005094353A3
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WO
WIPO (PCT)
Prior art keywords
regulatory
cells
production
methods
culture systems
Prior art date
Application number
PCT/US2005/010597
Other languages
English (en)
Other versions
WO2005094353A2 (fr
Inventor
Mark J Pykett
Michael Rosenzweig
Original Assignee
Cytomatrix Llc
Mark J Pykett
Michael Rosenzweig
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cytomatrix Llc, Mark J Pykett, Michael Rosenzweig filed Critical Cytomatrix Llc
Priority to EP05755497A priority Critical patent/EP1743025A4/fr
Priority to US10/594,806 priority patent/US20080063652A1/en
Priority to AU2005228442A priority patent/AU2005228442A1/en
Publication of WO2005094353A2 publication Critical patent/WO2005094353A2/fr
Publication of WO2005094353A3 publication Critical patent/WO2005094353A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0636—T lymphocytes
    • C12N5/0638—Cytotoxic T lymphocytes [CTL] or lymphokine activated killer cells [LAK]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2307—Interleukin-7 (IL-7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2315—Interleukin-15 (IL-15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/09—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells
    • C12N2502/094—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells keratinocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/11—Coculture with; Conditioned medium produced by blood or immune system cells
    • C12N2502/1185—Thymus cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2531/00—Microcarriers

Abstract

La présente invention concerne des systèmes de culture permettant la génération, l'expansion et l'isolation de lymphocytes T. régulateurs. Ces systèmes de culture peuvent être utilisés pour rechercher des facteurs qui modulent le développement des lymphocytes T régulateurs. Des lymphocytes T régulateurs dérivés de ces cultures peuvent être utilisés dans diverses applications in vitro et in vivo, de même que des populations de culture dépourvues de ces cellules.
PCT/US2005/010597 2004-03-29 2005-03-29 Techniques de production de lymphocytes t regulateurs et utilisation de ceux-ci WO2005094353A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP05755497A EP1743025A4 (fr) 2004-03-29 2005-03-29 Techniques de production de lymphocytes t regulateurs et utilisation de ceux-ci
US10/594,806 US20080063652A1 (en) 2004-03-29 2005-03-29 Methods for Production of Regulatory T Cells and Uses Thereof
AU2005228442A AU2005228442A1 (en) 2004-03-29 2005-03-29 Methods for production of regulatory T cells and uses thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US55766904P 2004-03-29 2004-03-29
US60/557,669 2004-03-29

Publications (2)

Publication Number Publication Date
WO2005094353A2 WO2005094353A2 (fr) 2005-10-13
WO2005094353A3 true WO2005094353A3 (fr) 2006-09-08

Family

ID=35064314

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/010597 WO2005094353A2 (fr) 2004-03-29 2005-03-29 Techniques de production de lymphocytes t regulateurs et utilisation de ceux-ci

Country Status (4)

Country Link
US (1) US20080063652A1 (fr)
EP (1) EP1743025A4 (fr)
AU (1) AU2005228442A1 (fr)
WO (1) WO2005094353A2 (fr)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
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ATE383417T1 (de) * 1998-11-12 2008-01-15 Cytomatrix Llc Produktion lymphoider gewebsspezifischer zellen von hämatopoietischen vorläuferzellen in einem dreidimensionalen system
CA2637686A1 (fr) * 2006-02-02 2007-08-16 Innovative Bio Therapies Dispositif therapeutique et systeme d'administration extracorporels a base de cellules
US8241621B2 (en) * 2006-12-18 2012-08-14 Medistem Laboratories Stem cell mediated treg activation/expansion for therapeutic immune modulation
WO2010019643A2 (fr) * 2008-08-15 2010-02-18 Innovative Biotherapies, Inc. Dispositif thérapeutique à base de cellules extracorporelles et système d'administration
WO2010124212A2 (fr) 2009-04-23 2010-10-28 The University Of Chicago Matériaux et procédés pour la préparation de nanocomposites
WO2011006029A1 (fr) 2009-07-10 2011-01-13 University Of Pittsburgh - Of The Commonwealth System Of Higher Education Produits de recombinaison cellulaire artificiels pour l’induction d’une tolérance immunitaire
US20120177618A1 (en) * 2009-07-14 2012-07-12 Massachusetts Institute Of Technology Hematopoietic stromal progenitor cells and uses thereof
GB0918615D0 (en) 2009-10-23 2009-12-09 Cellerix Sa Cell populations having immunoregulatory activity, methods for the preparation and uses thereof
WO2012135842A2 (fr) * 2011-03-31 2012-10-04 President And Fellows Of Harvard College Population unique de lymphocytes t régulateurs qui régulent la régénération de tissu et la cicatrisation des plaies
US9427450B2 (en) 2012-01-31 2016-08-30 Xon Cells, Inc. Therapeutic immune modulation by stem cell secreted exosomes
CN102693968B (zh) * 2012-05-25 2014-12-03 华为技术有限公司 芯片堆叠封装结构
EP3063271A4 (fr) * 2013-10-31 2017-05-03 Sloan-Kettering Institute for Cancer Research Méthodes et compositions pour induire une génération des lymphocytes t régulateurs
AU2014348454A1 (en) * 2013-11-15 2016-06-02 Celularity Inc. Compositions comprising human placental perfusate cells, subpopulations thereof, and their uses
US10092597B2 (en) 2014-01-14 2018-10-09 The University Of Hong Kong Human CD8+ regulatory T cells inhibit GVHD and preserve general immunity in humanized mice
JP7387603B2 (ja) * 2017-11-29 2023-11-28 フィジーン、エルエルシー 活性化のための線維芽細胞と免疫細胞との相互作用及びそれらの使用
CN111575299A (zh) * 2020-05-11 2020-08-25 吉林农业大学 牛荚膜a型多杀性巴氏杆菌免疫磁珠的制备方法

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US5541107A (en) * 1986-04-18 1996-07-30 Advanced Tissue Sciences, Inc. Three-dimensional bone marrow cell and tissue culture system

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US5635386A (en) * 1989-06-15 1997-06-03 The Regents Of The University Of Michigan Methods for regulating the specific lineages of cells produced in a human hematopoietic cell culture
US5763266A (en) * 1989-06-15 1998-06-09 The Regents Of The University Of Michigan Methods, compositions and devices for maintaining and growing human stem and/or hematopoietics cells
US5635387A (en) * 1990-04-23 1997-06-03 Cellpro, Inc. Methods and device for culturing human hematopoietic cells and their precursors
US5262320A (en) * 1990-06-18 1993-11-16 Massachusetts Institute Of Technology Cell-culturing apparatus and method employing a macroporous support
US5342774A (en) * 1991-05-23 1994-08-30 Ludwig Institute For Cancer Research Nucleotide sequence encoding the tumor rejection antigen precursor, MAGE-1
US5282861A (en) * 1992-03-11 1994-02-01 Ultramet Open cell tantalum structures for cancellous bone implants and cell and tissue receptors
US5677139A (en) * 1995-04-21 1997-10-14 President And Fellows Of Harvard College In vitro differentiation of CD34+ progenitor cells into T lymphocytes
US6121042A (en) * 1995-04-27 2000-09-19 Advanced Tissue Sciences, Inc. Apparatus and method for simulating in vivo conditions while seeding and culturing three-dimensional tissue constructs
US6440734B1 (en) * 1998-09-25 2002-08-27 Cytomatrix, Llc Methods and devices for the long-term culture of hematopoietic progenitor cells
US6548299B1 (en) * 1999-11-12 2003-04-15 Mark J. Pykett Lymphoid tissue-specific cell production from hematopoietic progenitor cells in three-dimensional devices
US6991933B1 (en) * 1999-09-24 2006-01-31 Cytomatrix, Llc Cell culture spinner flasks
JP2005527223A (ja) * 2002-05-24 2005-09-15 サイトマトリックス，エルエルシー． サイトカインフリーでの前駆細胞の増殖および維持

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Non-Patent Citations (1)

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Also Published As

Publication number Publication date
AU2005228442A2 (en) 2005-10-13
AU2005228442A1 (en) 2005-10-13
WO2005094353A2 (fr) 2005-10-13
US20080063652A1 (en) 2008-03-13
EP1743025A2 (fr) 2007-01-17
EP1743025A4 (fr) 2007-12-05

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