WO2005084420A1 - Materiau de culture pouvant induire des hybrides de ble en vue de produire des plantes diploides genetiquement pures et procede d'utilisation de ce materiau - Google Patents

Materiau de culture pouvant induire des hybrides de ble en vue de produire des plantes diploides genetiquement pures et procede d'utilisation de ce materiau Download PDF

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Publication number
WO2005084420A1
WO2005084420A1 PCT/CN2005/000156 CN2005000156W WO2005084420A1 WO 2005084420 A1 WO2005084420 A1 WO 2005084420A1 CN 2005000156 W CN2005000156 W CN 2005000156W WO 2005084420 A1 WO2005084420 A1 WO 2005084420A1
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WIPO (PCT)
Prior art keywords
wheat
breeding
plants
hybrids
plant
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Application number
PCT/CN2005/000156
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English (en)
Chinese (zh)
Inventor
Tianmin Shen
Original Assignee
Tianmin Shen
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Filing date
Publication date
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Publication of WO2005084420A1 publication Critical patent/WO2005084420A1/fr

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility

Definitions

  • the invention relates to a breeding material capable of inducing wheat hybrids to produce homozygous diploid plants.
  • the present invention also relates to a wheat breeding method for pollinating wheat hybrids or progeny with the breeding material, and inducing female parthenogenesis to develop into homozygous diploid plants. Background technique
  • % Is bred using conventional breeding methods % Is bred using conventional breeding methods.
  • ⁇ breeding of new wheat varieties by conventional breeding methods has a long separation time and slow stability, which usually takes 7-8 years, and distant hybridization has a longer stabilization time. Shortening the stabilization time, improving breeding efficiency, and speeding up the breeding process are the goals pursued by breeders in all countries.
  • haploid doubling to create a homozygous double haploid (DH) line has important practices for shortening the breeding period, accelerating the breeding process, improving selection efficiency and genetic research. significance.
  • DH homozygous double haploid
  • the induction frequency is low, mostly in the range of 0.3% to 5%, and the induced immature embryos need to be rescued (immature embryo culture) with the help of expensive medicines and experimental equipment, and the chromosomes are doubled after seedling formation.
  • the breeding unit is very Difficult to carry out, so the above methods have not been widely used in wheat breeding.
  • the invention provides a breeding material capable of inducing a heterozygous diploid plant in a wheat hybrid, a hexaploid triticale-inducing material. Using pollen of this inducing material to pollinate offspring of wheat hybrids can induce maternal parthenogenesis to develop into homozygous diploid plants.
  • the identification is Non-segregating lines are available, so the main selection process can be completed with only one selection of F2 plants. This method completely eliminates the process of artificial immature embryo culture and chromosome doubling.
  • the breeding material capable of inducing the production of homozygous diploid plants by wheat hybrids provided by the present invention is a hexaploid triticale-inducing material named "Lanyu No. 1".
  • the hexaploid triticale MZA-Lenonad BUTR was used as the female parent and Baofeng 7228 was used as the male parent.
  • the hexaploid small black was cultivated.
  • Wheat induction material "Lanyu No.1”. Characteristics of the breeding material "Lanyu No. 1"-plant height S 5 cm. Weak spring, medium cold resistance, early maturity, semi-upright seedlings, dark green leaves, short leaf width, medium tillering force, short and stout sting, medium spike rate. In adult stage, the plant type is compact, erect and erect, with good-looking stems.
  • the stems are stout, and the lower panicles account for three-fifths of the plant height.
  • the lower 1–13 knots are short, with thick stem walls and good toughness. Waxy.
  • the flag leaves are wide, short, thick, and deep in color.
  • the main veins protrude, and the angle of the second, third, and fourth leaves gradually increases.
  • the whole plant has a pagoda shape, a long functional period, and a good light receiving state.
  • the shell is white, the grains are round, the white horny, the seed coat is wrinkled, not full, and the thousand kernels weigh about 50 grams.
  • the root system is developed, and secondary roots occur in the first internode of the lower part. Strong vitality, drought resistance, stain resistance, high lodging resistance, high resistance to stripe rust, leaf rust, stalk rust, powdery mildew, root rot.
  • the method of inducing wheat hybrids to produce homozygous diploid plants using "Lanyu No. 1" is exactly the same as that of conventional wheat for compound hybridization, except that the material of "Lan Yu No. 1" must be used as the male parent.
  • the parental growth period used by the combination adjusts the seeding period to ensure that the flowering period meets. According to the breeding goal, select the combination or single plant with excellent performance in F1 or progeny, and use the "spider-wing hybrid method" to make 20 whole ears, with 30-40 florets per ear, and 600-800 florets per ear. When the stigma of the female parent is mature, pick the flowering spikelets in the middle of the "Lanyu No.
  • High-frequency induction can be used to select wheat substitution lines.
  • the invention also provides a breeding method for inducing wheat hybrids to produce homozygous diploid plants.
  • the method is to use conventional breeding methods to pollinate wheat hybrids or progeny (including distant hybrid materials) with wheat exogenous materials (ie, the aforementioned hexaploid triticale "Lanyu No. 1") to induce female parthenogenesis in F1
  • a breeding method that develops into a homozygous diploid plant so that hybrid progeny can no longer be separated.
  • the induction frequency can reach 10. 20-81. 56%.
  • the breeding procedure for induction of homozygous diploid plants in wheat hybrids using the "Lanyu No. 1" material is as follows: The first year, choose hybrid F1 or
  • Progeny single plants are combined, and each combination is integrated with lanlan No. 1
  • Homozygous diploid plants can be obtained in the F1 generation of hybrids, and the progeny are no longer separated.

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

Cette invention se rapporte à un matériau de culture qui peut induire des hybrides de blé, en vue de produire des plantes diploïdes génétiquement pures, ainsi qu'à un procédé d'utilisation de ce matériau pour produire des lignées de blé pures. Ce matériau de culture est constitué par une triticale hexaploïde. Ce procédé utilise des pollens provenant de la triticale hexaploïde en tant qu'hybrides de blé émasculé de pollinisation de parent mâle ou leur descendance comme parent femelle. Tous les hybrides F1 provenant des parents femelles induits sont des plantes à diploïdie pure. La descendance de ces hybrides ne présente pas de ségrégation. Ce procédé peut induire efficacement la production de plantes de blé diploïdes pures et ne produit pas de plantes haploïdes. Ce procédé peut fixe la vigueur ou l'hétérosis des hybrides de blé, et il réduit la période de culture. Ce procédé adopte des moyens de culture traditionnels et ne nécessite pas de produits chimiques et d'équipements coûteux.
PCT/CN2005/000156 2004-03-05 2005-02-04 Materiau de culture pouvant induire des hybrides de ble en vue de produire des plantes diploides genetiquement pures et procede d'utilisation de ce materiau WO2005084420A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN200410004762.5 2004-03-05
CNA2004100047625A CN1559186A (zh) 2004-03-05 2004-03-05 诱导小麦杂种产生纯合二倍体植株的育种材料和方法

Publications (1)

Publication Number Publication Date
WO2005084420A1 true WO2005084420A1 (fr) 2005-09-15

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PCT/CN2005/000156 WO2005084420A1 (fr) 2004-03-05 2005-02-04 Materiau de culture pouvant induire des hybrides de ble en vue de produire des plantes diploides genetiquement pures et procede d'utilisation de ce materiau

Country Status (2)

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CN (1) CN1559186A (fr)
WO (1) WO2005084420A1 (fr)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102972284A (zh) * 2012-11-19 2013-03-20 河南大学 一种获得人工合成六倍体小麦的新方法
CN108184657A (zh) * 2018-02-27 2018-06-22 姜新 一种黑金谷的育种方法
CN115553211A (zh) * 2022-08-26 2023-01-03 河北省农林科学院生物技术与食品科学研究所 一种小麦杂交快速获得纯系的育种方法
CN116762688A (zh) * 2023-07-17 2023-09-19 滨州市农业科学院 一种促进燕麦和小麦杂交花期相遇的调节方法

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102334448A (zh) * 2010-07-23 2012-02-01 沈天民 一种小麦的工厂化培育方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1185275A (zh) * 1996-12-17 1998-06-24 中国农业科学院作物育种栽培研究所 抗小麦黄矮病毒病小麦种质的育种方法
CN1266615A (zh) * 2000-04-10 2000-09-20 湖北大学生命科学学院 利用远缘杂交和多倍体双重优势选育水稻新品种和杂交水稻的方法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1185275A (zh) * 1996-12-17 1998-06-24 中国农业科学院作物育种栽培研究所 抗小麦黄矮病毒病小麦种质的育种方法
CN1266615A (zh) * 2000-04-10 2000-09-20 湖北大学生命科学学院 利用远缘杂交和多倍体双重优势选育水稻新品种和杂交水稻的方法

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102972284A (zh) * 2012-11-19 2013-03-20 河南大学 一种获得人工合成六倍体小麦的新方法
CN108184657A (zh) * 2018-02-27 2018-06-22 姜新 一种黑金谷的育种方法
CN115553211A (zh) * 2022-08-26 2023-01-03 河北省农林科学院生物技术与食品科学研究所 一种小麦杂交快速获得纯系的育种方法
CN116762688A (zh) * 2023-07-17 2023-09-19 滨州市农业科学院 一种促进燕麦和小麦杂交花期相遇的调节方法
CN116762688B (zh) * 2023-07-17 2024-03-29 滨州市农业科学院 一种促进燕麦和小麦杂交花期相遇的调节方法

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Publication number Publication date
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