WO2005063966A3 - Verfahren zur in vitro differenzierung neuronaler stammzellen oder von neuronalen stammzellen abgeleiteter zellen - Google Patents

Verfahren zur in vitro differenzierung neuronaler stammzellen oder von neuronalen stammzellen abgeleiteter zellen Download PDF

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Publication number
WO2005063966A3
WO2005063966A3 PCT/EP2004/014673 EP2004014673W WO2005063966A3 WO 2005063966 A3 WO2005063966 A3 WO 2005063966A3 EP 2004014673 W EP2004014673 W EP 2004014673W WO 2005063966 A3 WO2005063966 A3 WO 2005063966A3
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WO
WIPO (PCT)
Prior art keywords
cells
neuronal stem
stem cells
vitro differentiation
derived
Prior art date
Application number
PCT/EP2004/014673
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English (en)
French (fr)
Other versions
WO2005063966A2 (de
Inventor
Martin H Maurer
Robert E Feldmann
Wolfgang Kuschinsky
Armin Schneider
Original Assignee
Axaron Bioscience Ag
Martin H Maurer
Robert E Feldmann
Wolfgang Kuschinsky
Armin Schneider
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Axaron Bioscience Ag, Martin H Maurer, Robert E Feldmann, Wolfgang Kuschinsky, Armin Schneider filed Critical Axaron Bioscience Ag
Priority to US10/584,341 priority Critical patent/US20090081189A1/en
Priority to EP04804267A priority patent/EP1697501A2/de
Priority to CA002551259A priority patent/CA2551259A1/en
Priority to AU2004309070A priority patent/AU2004309070A1/en
Publication of WO2005063966A2 publication Critical patent/WO2005063966A2/de
Publication of WO2005063966A3 publication Critical patent/WO2005063966A3/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0619—Neurons
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/005—Enzyme inhibitors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/1703—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • A61K38/1709—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5023—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on expression patterns
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5041—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects involving analysis of members of signalling pathways
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5058—Neurological cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5073—Stem cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6893—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere
    • G01N33/6896—Neurological disorders, e.g. Alzheimer's disease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled

Abstract

Das Verfahren zur in vitro Differenzierung von neuronalen Stammzellen umfasst das in Kontakt bringen der Zellen mit einer Substanz, die eine Reaktion des Wnt­Signaltransduktionswegs inhibiert, und das Kultivieren dieser Zellen unter Bedingungen, die eine Vermehrung und/oder Differenzierung der Zellen ermöglichen. In einer bevorzugten Ausführungsform des Verfahrens differenzieren die neuronalen Stammzellen zu gehirnzellenähnlichen Zellen.
PCT/EP2004/014673 2003-12-23 2004-12-23 Verfahren zur in vitro differenzierung neuronaler stammzellen oder von neuronalen stammzellen abgeleiteter zellen WO2005063966A2 (de)

Priority Applications (4)

Application Number Priority Date Filing Date Title
US10/584,341 US20090081189A1 (en) 2003-12-23 2004-12-23 Process for in vitro differentiation of neuronal stem cells or of cells derived from neuronal stem cells
EP04804267A EP1697501A2 (de) 2003-12-23 2004-12-23 Verfahren zur in vitro differenzierung neuronaler stammzellen oder von neuronalen stammzellen abgeleiteter zellen
CA002551259A CA2551259A1 (en) 2003-12-23 2004-12-23 A process for in vitro differentiation of neuronal stem cells or of cells derived from neuronal stem cells
AU2004309070A AU2004309070A1 (en) 2003-12-23 2004-12-23 Method for in vitro differentiation of neuronal stem cells or cells derived from neuronal stem cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10361444A DE10361444A1 (de) 2003-12-23 2003-12-23 Verfahren zur in vitro Differenzierung neuronaler Stammzellen oder von neuronalen Stammzellen abgeleiteter Zellen
DE10361444.3 2003-12-23

Publications (2)

Publication Number Publication Date
WO2005063966A2 WO2005063966A2 (de) 2005-07-14
WO2005063966A3 true WO2005063966A3 (de) 2005-10-27

Family

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PCT/EP2004/014673 WO2005063966A2 (de) 2003-12-23 2004-12-23 Verfahren zur in vitro differenzierung neuronaler stammzellen oder von neuronalen stammzellen abgeleiteter zellen

Country Status (6)

Country Link
US (1) US20090081189A1 (de)
EP (1) EP1697501A2 (de)
AU (1) AU2004309070A1 (de)
CA (1) CA2551259A1 (de)
DE (1) DE10361444A1 (de)
WO (1) WO2005063966A2 (de)

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CA2617601A1 (en) * 2004-09-02 2006-03-09 Neuro Therapeutics Ab Methods and materials relating to enhanced production of dopamine neurons
WO2007016485A2 (en) * 2005-07-29 2007-02-08 Athersys, Inc. Use of a gsk-3 inhibitor to maintain potency of cultured cells
WO2010014622A2 (en) * 2008-07-28 2010-02-04 Fondazione Istituto Firc Di Oncologica Molecolare (Ifom) In vitro model for modulating the blood brain barrier and methods of screening
WO2010052203A1 (en) * 2008-11-04 2010-05-14 Novartis Forschungsstiftung, Zweigniederlassung, Friedrich Miescher Institute For Biomedical Research Modulating neuronal plasticity and treatment of neuronal loss by modulating wnt7a or wnt7b modulation
CN111690612B (zh) * 2019-02-28 2023-07-25 同济大学 通过调控Wnt信号和/或Notch信号扩增人神经前体细胞的方法
CN114292815A (zh) * 2022-01-08 2022-04-08 广东省疾病预防控制中心 一种人神经祖细胞分化方法及其在毒性测试中的应用

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US6686198B1 (en) * 1993-10-14 2004-02-03 President And Fellows Of Harvard College Method of inducing and maintaining neuronal cells

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Also Published As

Publication number Publication date
DE10361444A1 (de) 2005-07-21
US20090081189A1 (en) 2009-03-26
WO2005063966A2 (de) 2005-07-14
EP1697501A2 (de) 2006-09-06
AU2004309070A1 (en) 2005-07-14
CA2551259A1 (en) 2005-07-14

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