WO2005060345A3 - New method for sequencing-by-synthesis - Google Patents

New method for sequencing-by-synthesis Download PDF

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Publication number
WO2005060345A3
WO2005060345A3 PCT/IB2004/004438 IB2004004438W WO2005060345A3 WO 2005060345 A3 WO2005060345 A3 WO 2005060345A3 IB 2004004438 W IB2004004438 W IB 2004004438W WO 2005060345 A3 WO2005060345 A3 WO 2005060345A3
Authority
WO
WIPO (PCT)
Prior art keywords
sequencing
synthesis
tubular member
methods
new method
Prior art date
Application number
PCT/IB2004/004438
Other languages
French (fr)
Other versions
WO2005060345A2 (en
Inventor
Nigel Tooke
Peter Hagerlid
Bjorn Ekstrom
Original Assignee
Biotage Ab
Nigel Tooke
Peter Hagerlid
Bjorn Ekstrom
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from SE0303473A external-priority patent/SE0303473D0/en
Application filed by Biotage Ab, Nigel Tooke, Peter Hagerlid, Bjorn Ekstrom filed Critical Biotage Ab
Publication of WO2005060345A2 publication Critical patent/WO2005060345A2/en
Publication of WO2005060345A3 publication Critical patent/WO2005060345A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The invention refers to a tubular member, such as a pipette tip, for binding nucleic acid molecules for a subsequent biomolecular reaction, to methods for at least partly performing a sequencing-by-synthesis reaction in a tubular member, to an automated system using the tubular member for performing the methods of the invention, and kits and computer programs for use in relation to the methods of the invention. By performing a sequencing-by-synthesis reaction in a tubular member, such as a pipette tip, a new format for enzymatic reactions of this type, i.e. sequencing by synthesis, is provided.
PCT/IB2004/004438 2003-12-22 2004-12-20 New method for sequencing-by-synthesis WO2005060345A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
SE0303473-3 2003-12-22
SE0303473A SE0303473D0 (en) 2003-12-22 2003-12-22 New method
US53162003P 2003-12-23 2003-12-23
US60/531,620 2003-12-23

Publications (2)

Publication Number Publication Date
WO2005060345A2 WO2005060345A2 (en) 2005-07-07
WO2005060345A3 true WO2005060345A3 (en) 2006-05-11

Family

ID=34713080

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2004/004438 WO2005060345A2 (en) 2003-12-22 2004-12-20 New method for sequencing-by-synthesis

Country Status (1)

Country Link
WO (1) WO2005060345A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7754429B2 (en) 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
MX2012004627A (en) 2009-10-22 2012-05-23 Biotools Biotechnological & Medical Lab S A Composition, method and kit for detecting bacteria by means of sequencing.

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5171537A (en) * 1991-05-06 1992-12-15 Richard E. MacDonald Activated immunodiagnostic pipette tips
WO1994020831A1 (en) * 1993-03-08 1994-09-15 Norman Wainwright Aligned fiber diagnostic chromatography
WO2001085341A1 (en) * 2000-05-12 2001-11-15 Pyrosequencing Ab Microfluidic devices
WO2003068962A1 (en) * 2002-02-12 2003-08-21 Biotage Ab Separating method and an apparatus performing such a method
WO2003085135A1 (en) * 2002-04-04 2003-10-16 Biotage New method
WO2003102234A1 (en) * 2002-06-03 2003-12-11 Pamgene Bv Method for high-throughput integrated chemical and biochemical reactions

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5171537A (en) * 1991-05-06 1992-12-15 Richard E. MacDonald Activated immunodiagnostic pipette tips
WO1994020831A1 (en) * 1993-03-08 1994-09-15 Norman Wainwright Aligned fiber diagnostic chromatography
WO2001085341A1 (en) * 2000-05-12 2001-11-15 Pyrosequencing Ab Microfluidic devices
WO2003068962A1 (en) * 2002-02-12 2003-08-21 Biotage Ab Separating method and an apparatus performing such a method
WO2003085135A1 (en) * 2002-04-04 2003-10-16 Biotage New method
WO2003102234A1 (en) * 2002-06-03 2003-12-11 Pamgene Bv Method for high-throughput integrated chemical and biochemical reactions

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
ANDERSSON H ET AL: "Micromachined filter-chamber array with passive valves for biochemical assays on beads.", ELECTROPHORESIS. JAN 2001, vol. 22, no. 2, January 2001 (2001-01-01), pages 249 - 257, XP002359035, ISSN: 0173-0835 *
POURMAND N ET AL: "Multiplex Pyrosequencing", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 30, no. 7, 2002, pages E31 - 1, XP002329580, ISSN: 0305-1048 *
RUSSOM AMAN ET AL: "Single nucleotide polymorphism analysis by allele-specific primer extension with real-time bioluminescence detection in a microfluidic device.", JOURNAL OF CHROMATOGRAPHY A, vol. 1014, no. 1-2, 3 October 2003 (2003-10-03), pages 37 - 45, XP004456338, ISSN: 0021-9673 *

Also Published As

Publication number Publication date
WO2005060345A2 (en) 2005-07-07

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