WO2005044923A1 - Cyanine dye labelling reagents - Google Patents
Cyanine dye labelling reagents Download PDFInfo
- Publication number
- WO2005044923A1 WO2005044923A1 PCT/GB2004/004573 GB2004004573W WO2005044923A1 WO 2005044923 A1 WO2005044923 A1 WO 2005044923A1 GB 2004004573 W GB2004004573 W GB 2004004573W WO 2005044923 A1 WO2005044923 A1 WO 2005044923A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- groups
- group
- dye
- methyl
- sulphobutyl
- Prior art date
Links
- AZZWNFWTJHSJFZ-UHFFFAOYSA-N CC1(CCCCS(O)(=O)=O)c(cc(CC(O)=O)cc2)c2N=C1C Chemical compound CC1(CCCCS(O)(=O)=O)c(cc(CC(O)=O)cc2)c2N=C1C AZZWNFWTJHSJFZ-UHFFFAOYSA-N 0.000 description 1
- KGECGSDLSUJXJG-UHFFFAOYSA-N CC1(CCCCS(O)(=O)=O)c2cc(S(O)(=O)=O)ccc2N=C1C Chemical compound CC1(CCCCS(O)(=O)=O)c2cc(S(O)(=O)=O)ccc2N=C1C KGECGSDLSUJXJG-UHFFFAOYSA-N 0.000 description 1
- HZNYUTUMAQYLKI-UHFFFAOYSA-N CCC(CC(C=C1)=O)C1=O Chemical compound CCC(CC(C=C1)=O)C1=O HZNYUTUMAQYLKI-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
- C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
- C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
- C07D209/04—Indoles; Hydrogenated indoles
- C07D209/10—Indoles; Hydrogenated indoles with substituted hydrocarbon radicals attached to carbon atoms of the hetero ring
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
- C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
- C07D209/04—Indoles; Hydrogenated indoles
- C07D209/10—Indoles; Hydrogenated indoles with substituted hydrocarbon radicals attached to carbon atoms of the hetero ring
- C07D209/18—Radicals substituted by carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals
- C07D209/24—Radicals substituted by carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals with an alkyl or cycloalkyl radical attached to the ring nitrogen atom
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D403/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00
- C07D403/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing two hetero rings
- C07D403/06—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing two hetero rings linked by a carbon chain containing only aliphatic carbon atoms
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D403/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00
- C07D403/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing two hetero rings
- C07D403/08—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing two hetero rings linked by a carbon chain containing alicyclic rings
Definitions
- the present invention relates to the field of labelling reagents, in particular reactive cyanine dyes having one or multiple water solubilising groups attached thereon and to methods utilising such dyes.
- Fluorescent labels are established as the detection means of choice in microarray analysis.
- Post-labelling strategies in microarray analyses have the potential to offer improved sensitivity of detection, especially for low expressed targets and enable the use of less mRNA.
- ultrasensitive detection methods such as may be obtained through the use of multiple labels, either through chemical labelling of the nucleic acid molecule, or through the use of avidin or streptavidin conjugates.
- Cyanine dyes offer a number of advantages over other fluorescent dyes.
- the excitation and emission spectra of cyanine dyes span the visible and NIR spectrum from 450nm to 800nm.
- the cyanine dyes are characterised by having very high extinction coefficients, favourable quantum yields and good photostability. See for example, US Patent Nos.6048982, 5268486, 5569587, (Waggoner, A.S. et al).
- WO 02/26891 (Molecular Probes Inc.) describes modified carbocyanine dyes and their conjugates with target materials, in which there is at least one substituted indolinium ring system, where the substituent on the 3-position of the indolinium ring contains a chemically reactive group or a conjugated substance.
- the modified dyes according to WO 02/26891 are reported to overcome the tendency of cyanine dyes to self-associate (i.e. stack) and dye conjugates labelled with the modified dyes are reported to be more fluorescent than conjugates labelled with structurally similar carbocyanine dyes.
- US Patent No. 6083485 (Licha et al) relates to an in-vivo diagnostic method based on near infra-red radiation (NIR) that uses dyes having the following structure:
- groups X and Y include the groups -C(CH 2 R 32 )(CH 2 R 33 ) and groups R 20 to R 29 , R 32 and R 33 may be substituted with groups including hydroxy, carboxy, sulphonic acid, carboxyalkyl, alkoxycarbonyl or alkoxyoxoalkyl residues containing up to 10 carbon atoms, or a sulphoalkyl residue containing up to 4 carbon atoms.
- Japanese Patent Application No. 5313304 discloses a silver halide photographic sensitive material incorporating a dye containing multiple sulphonate groups and represented by the formula:
- R a and R b may be alkyl carboxylate or alkyl sulphonate moieties.
- None of the prior art documents specifically discloses a cyanine dye having one or more sulphonic acid or phosphonic acid water solubilising groups attached to the 3-position of the indolinium ring system, in which dye there is also provided at least one group suitable for direct covalent or non-covalent labelling of a target material. It has now been found that a new class of cyanine dye labelling reagents are useful for labelling and detecting biological and other materials.
- Z 1 and Z 2 independently represent the carbon atoms necessary to complete a one ring, or two-fused ring aromatic system; at least one of groups R 1 , R 2 , R 3 , R 4 , R 5 , R 6 and R 7 is the group -E-F where E is a single bond or a spacer group having a chain from 1-20 linked atoms selected from the group consisting of carbon, nitrogen and oxygen atoms and F is a target bonding group; one or more of groups R 11 , R 12 , R 13 and R 14 are independently selected from the group -(Chb ⁇ -W, where W is sulphonic acid or phosphonic acid and k is an integer from 1 to 10; when any of groups R 1 and R 2 is not said group -E-F, said remaining groups
- R 1 and R 2 are independently selected from Ci - C 6 alkyl, benzyl either unsubstituted or substituted with sulphonic acid, and the group -(CH 2 ) k -W, where W and k are hereinbefore defined; when any of groups R 3 , R 4 , R 5 and R 6 is not said group -E-F, said remaining groups R 3 , R 4 , R 5 and R 6 are independently selected from hydrogen and sulphonic acid; when any of groups R 11 , R 12 , R 13 and R 14 is not said group -(CH 2 ) k -W, said remaining groups R 11 , R 12 , R 13 and R 14 are independently Ci - C 6 alkyl; remaining groups R 7 are hydrogen or two of R 7 together with the group,
- the compound according to the first aspect includes one or more counter-ions, which may be positive or negative to balance the formal charge (or charges) on the dye chromophore.
- the nature of the counter-ion is not material to the invention and could be one of many known ions such as NH 4 + , K + , Na + , trifluoroacetate (F 3 C-C0 2 ⁇ ), perchlorate (CI0 ⁇ ), Br ⁇ , or I " .
- the terms “sulphonic acid” and “phosphonic acid” will also include respectively the groups “sulphonate” and “phosphonate", since they are the ionised forms of the parent acids.
- At least two of groups R 11 , R 2 , R 13 and R 14 are the group -(CH 2 ) k -W.
- one of groups R 11 and R 12 , and one of groups R 13 and R 14 is the group -(CH 2 )k-W, wherein W and k are hereinbefore defined.
- remaining groups R 11 or R 12 and R 13 or R 14 are preferably methyl.
- compounds of the present invention are those in which W is sulphonic acid.
- k is 3 or 4.
- Particularly preferred -(CH 2 ) k -W is selected from -(CH 2 ) 3 -S0 3 H and -(CH 2 ) 4 -S0 3 H.
- said remaining groups R 1 and R 2 may be selected from Ci - C 6 alkyl, benzyl either unsubstituted or substituted with sulphonic acid, and the group -(CH 2 ) k -W, where W and k are hereinbefore defined.
- said remaining groups R 1 and R 2 may be selected from C-i - C ⁇ alkyl, sulphobenzyl and the group -(CH 2 ) k -W.
- preferred alkyl groups are methyl and ethyl.
- Z 1 and Z 2 are independently selected from phenyl and naphthyl.
- Particular examples of cyanine dyes according to the compound of formula (I) and having one or two fused ring aromatic systems are shown as structures (II), (III), (IV), (V) and (VI) in Table 1.
- Table 1
- Examples of compounds in which groups R 7 form a hydrocarbon ring system are shown in Table 2 as structures (VII) and (VIII).
- structures (II) to (VIII) groups R 1 , R 2 , R 3 , R 4 , R 5 , R 6 , R 7 , R 11 , R 12 , R 13 , R 14 and n are as hereinbefore defined.
- the linking moiety E links the target bonding group F with the chromophore moiety of the compounds according to formula (I).
- the target bonding group F may be attached directly to the R 3 , R 4 , R 5 , R 6 or R 7 positions of the dye, in which case E is a single covalent bond.
- the target bonding group F may be covalently attached to the R 1 , R 2 , R 3 , R 4 , R 5 , R 6 or R 7 positions of the dye indirectly, via a spacer group.
- E is suitably a straight or branched chain of from 1 to 20 linked atoms containing carbon, nitrogen and oxygen atoms.
- the spacer group E is selected from:
- the target bonding group F is a group that reacts with a complementary group of a target component, with the formation of a covalent linkage between the dye and the component.
- the choice of bonding group will depend on the groups that are available on the component to be labelled and, as such, will be well known to those skilled in the art.
- the target bonding group may be a reactive group that can react under suitable conditions with a complementary functional group of a component.
- functional groups present in components such as proteins, peptides, nucleic acids carbohydrates and the like, include hydroxy, amino, sulphydryl, carbonyl (including aldehyde and ketone) and thiophosphate.
- the target bonding group F may be a functional group and the target may contain, or be derivatised to contain a reactive constituent, such that the functional group of the dye may be reacted under suitable conditions with the reactive group of the target component.
- the component becomes labelled with the dye according to formula (I).
- reactive groups F may be selected from carboxyl, succinimidyl ester, sulpho-succinimidyl ester, isothiocyanate, maleimide, haloacetamide, acid halide, hydrazide, vinylsulphone, dichlorotriazine and phosphoramidite.
- the reactive group is a succinimidyl ester of a carboxylic acid, an isothiocyanate, a maleimide, a haloacetamide or a phosphoramidite.
- F is a functional group, it is suitably selected from hydroxy, amino, sulphydryl, carbonyl (including aldehyde and ketone) and thiophosphate.
- R 1 , R 2 , R 3 , R 4 , R 5 , R 6 and R 7 in the compound according to formula (I) and the groups with which groups R 1 , R 2 , R 3 , R 4 , R 5 , R 6 and R 7 can react are provided in Table 3.
- R 1 , R 2 , R 3 , R 4 , R 5 , R 6 and R 7 may be the functional groups of Table 3 which would react with the reactive groups of a target component.
- Particularly preferred reactive groups R 1 , R 2 , R 3 , R 4 , R 5 , R 6 and R 7 which are especially useful for labelling target components with available amino and hydroxyl functional groups include:
- Particularly preferred reactive groups R 1 , R 2 , R 3 , R 4 , R 5 , R 6 and R 7 which are useful for labelling target components with available thiol functional groups include:
- group -E-F are those which comprise a carboxypentyl group E, for example:
- the target bonding group F may be an affinity tag which is capable of binding specifically and non-covalently with its complementary specific binding partner.
- specific binding partner pairs include, but are not restricted to: biotin/avidin, biotin/streptavidin, polyhistidine tag-metal ion complexes with nitrilotriacetic acid (e.g. Ni 2+ : NTA).
- the complementary specific binding partner may be one component of a labelling complex for detection of a target component.
- streptavidin having four sites of attachment for a biotin label
- group F is biotin, iminobiotin or desthiobiotin.
- group F is biotin, iminobiotin or desthiobiotin.
- affinity tags are selected from biotin, iminobiotin and desthiobiotin.
- the fluorescent cyanine dyes of the present invention may contain one or more additional sulphonic acid groups.
- one or more sulphonic acid groups may be attached directly to the Z 1 and/or Z 2 ring structures.
- the R 1 and/or R 2 positions may be substituted directly with sulphobenzyl or the group -(CH 2 ) k -W , where W and k are hereinbefore defined.
- the dye may be optionally further substituted with one or more sulphonic acid groups attached directly to the R 3 , R 4 , R 5 and R ⁇ positions.
- the dyes according to the present invention may be substituted with up to five or more sulphonic acid groups, preferably between three and five sulphonic acid groups.
- the use of cyanine dyes substituted with three or more sulphonic acid groups for labelling biological target molecules results in a labelled product in which there is reduced dye-dye aggregation, negligible excited state interactions and therefore minimal dye-dye quenching and loss of fluorescence.
- the fluorescence emission intensity of a molecule so labelled with the preferred dyes of the present invention increases with the number of covalently attached dyes.
- substitution of the indolinium 3-position with sulphonic acid groups in addition to increasing the overall charge on the dye molecule also adds steric bulk, thereby contributing to a reduction in dye-dye aggregation.
- Halogen and halo groups are selected from fluorine, chlorine, bromine and iodine.
- n 1 , 2 or 3; at least one of groups R 1 , R 2 , R 3 and R 5 is the group -E-F where E and F are hereinbefore defined; when any of groups R 1 and R 2 is not said group -E-F, said remaining groups R 1 and R 2 are independently selected from methyl, ethyl and -(CH 2 ) k -W, where W is sulphonic acid and k is 3 or 4; when any of groups R 3 and R 5 is not said group -E-F, said remaining groups R 3 and R 5 are independently selected from hydrogen and sulphonic acid, preferably sulphonic acid.
- group -E-F is suitably a succinimidyl ester derivative of an alkyl carboxylic acid, preferably 5-carboxypentyl, N- hydroxysuccinimidyl ester, or 5-carboxypentyl, N-hydroxy-sulphosuccinimidyl ester.
- the present invention also relates to labelling methods wherein the compounds of the present invention including at least group F attached to the R 1 to R 7 positions as hereinbefore defined may be used to label and thereby impart fluorescent properties to a target component.
- they may be used for multiple labelling and detection of biological molecules, such as nucleic acids, DNA, RNA, oligonucleotides, nucleotides, proteins, peptides, antibodies, etc.
- a method for labelling a component comprising: i) contacting said component with a compound of formula (I):
- Z 1 and Z 2 independently represent the carbon atoms necessary to complete a one ring, or two-fused ring aromatic system; at least one of groups R 1 , R 2 , R 3 , R 4 , R 5 , R 6 and R 7 is the group -E-F where E is a single bond or a spacer group having a chain from 1-20 linked atoms selected from the group consisting of carbon, nitrogen and oxygen atoms and F is a target bonding group; one or more of groups R 11 , R 12 , R 13 and R 14 are independently selected from the group -(CH 2 ) k -W, where W is sulphonic acid or phosphonic acid and k is an integer from 1 to 10; when any of groups R 1 and R 2 is not said group -E-F, said remaining groups
- R 1 and R 2 are independently selected from Ci - C ⁇ alkyl, benzyl either unsubstituted or substituted with sulphonic acid, and the group -(CH 2 ) k -W, where W and k are hereinbefore defined; when any of groups R 3 , R 4 , R 5 and R 6 is not said group -E-F, said remaining groups R 3 , R 4 , R 5 and R 6 are independently selected from hydrogen and sulphonic acid; when any of groups R 11 , R 12 , R 13 and R 14 is not said group -(CH 2 ) k ⁇ W, said remaining groups R 11 , R 12 , R 13 and R 14 are independently Ci - C ⁇ alkyl; remaining groups R 7 are hydrogen or two of R 7 together with the group, R 7
- the target bonding group F may be a group suitable for the formation of a covalent link between the compound of formula (I) and the target component, such as a reactive or functional group as hereinbefore defined.
- the target bonding group F is an affinity tag, for example biotin, desthiobiotin or iminobiotin, and the dye is bound to the target by non-covalent association.
- the method comprises incubating the component to be labelled with an amount of the compound according to the invention under conditions such that the dye becomes bound to the component. Methods for the formation of dye conjugates or complexes with target components will be well known to the skilled person.
- covalent labelling of proteins is typically performed in an aqueous buffered medium, suitably bicarbonate at pH 9.0, at ambient temperature for a period of typically 1 hour.
- the reaction is normally carried out in the dark.
- the labelled protein can be separated from any unreacted dye by size exclusion chromatography, for example using SephadexTM as the stationary phase and phosphate buffer, pH 7.0 as the eluant.
- size exclusion chromatography for example using SephadexTM as the stationary phase and phosphate buffer, pH 7.0 as the eluant.
- the ratio of the amount or concentration of dye to target material should be adjusted accordingly.
- Suitable target biological components include, but are not limited to the group consisting of antibody, lipid, protein, peptide, carbohydrate, nucleotides which contain or are derivatized to contain one or more of an amino, sulphydryl, carbonyl, hydroxyl and carboxyl and thiophosphate groups, and oxy or deoxy polynucleic acids which contain or are derivatized to contain one or more of an amino, sulphydryl, carbonyl, hydroxyl, carboxyl and thiophosphate groups, microbial materials, drugs, hormones, cells, cell membranes and toxins.
- the present invention also relates to two-step labelling processes in which, in a first step, a dye according to the present invention binds to, and thereby labels a primary component, such as an antibody, protein, DNA probe, etc.
- a primary component such as an antibody, protein, DNA probe, etc.
- the fluorescently labelled primary component is then used as a probe for detection of a secondary component, such as an antigen for which the antibody is specific.
- the compounds of the present invention can also be used to determine the concentration of a particular protein or other component in a system. If the number of reactive groups on a protein which can react with a probe is known, the fluorescence per molecule can be known and the concentration of these molecules in the system can be determined by the total fluorescence intensity of the system. This particular method can be used to measure the concentration of various labelled analytes using microtitre plate readers or other known immunofluorescence detection systems. The concentration of fluorescently labelled material can also be determined using, for example, fluorescence polarization detection instruments.
- the compounds of the present invention may also be used in a detection method wherein a plurality of the fluorescent dyes are covalently attached to a plurality of different primary components, such as antibodies, each primary component being specific for a different secondary component, such as an antigen, in order to identify each of a plurality of secondary components in a mixture of secondary components.
- each of the primary components is separately labelled with a fluorescent dye having a different light absorption and emission wavelength characteristic, compared with the dye molecules used for labelling the other primary components.
- the labelled primary components are then added to the preparation containing secondary components, such as antigens, and the primary components are allowed to attach to the respective secondary components for which they are selective.
- any unreacted probe materials may be removed from the preparation by, for example, washing, to prevent interference with the analysis.
- the preparation is then subjected to a range of excitation wavelengths including the absorption wavelengths of particular fluorescent compounds.
- a fluorescence microscope or other fluorescence detection system such as a flow cytometer or fluorescence spectrophotometer, having filters or monochromators to select the rays of the excitation wavelength and to select the wavelengths of fluorescence is next employed to determine the intensity of the emission wavelengths corresponding to the fluorescent compounds utilized, the intensity of fluorescence indicating the quantity of the secondary component which has been bound with a particular labelled primary component.
- Known techniques for conducting multi-parameter fluorescence studies include, for example, multiparameter flow cytometry.
- a single wavelength of excitation can be used to excite fluorescence from two or more materials in a mixture where each fluoresces at a different wavelength and the quantity of each labelled species can be measured by detecting its individual fluorescence intensity at its respective emission wavelength.
- a light absorption method can also be employed.
- the detection method of the present invention can be applied to any system in which the creation of a fluorescent primary component is possible.
- an appropriately reactive fluorescent compound can be conjugated to a DNA or RNA fragment and the resultant conjugate then caused to bind to a complementary target strand of DNA or RNA.
- Appropriate fluorescence detection equipment can then be employed to detect the presence of bound fluorescent conjugates.
- the present invention relates to intermediates and to methods useful for preparing the dyes of formula (I) which are suitably prepared by a process comprising: a) reacting a first intermediate compound having the formula (A):
- Z 2 , R 2 , R 5 , R 6 , R 13 and R 14 are hereinbefore defined, and c) a third compound (C) suitable for forming a linkage between the first and second compounds; provided that at least one of the groups R 1 , R 2 , R 3 , R 4 , R 5 and R 6 is the group -E-F, where E and F are hereinbefore defined; and provided that one or more of groups R 11 , R 12 , R 13 and R 14 are independently selected from the group -(CH 2 ) k -W, where W is selected from sulphonic acid and phosphonic acid groups and k is an integer from 1 to 10.
- -(CH 2 ) k -W is selected from -(CH 2 ) 3 -S0 3 H and -(CH 2 ) 4 -S0 3 H.
- intermediate compounds (A), (C) and (B) may be reacted either in a single step or in a multiple step process to form the compounds of formula (I).
- Symmetrical compounds of formula (I) wherein structures (A) and (B) are the same may be suitably prepared by reacting a compound of formula (A) (or (B)) in two molar proportions with an appropriate bis-functional methine fragment containing 1 , 3 or 5 carbon atoms, substituted with a group to form R 7 as hereinbefore defined.
- a substituted N.N'-diphenylformamidine, or ortho ester will be employed as the third compound (C) for preparing trimethine cyanine dye analogues.
- a suitably substituted malondialdehyde dianil may be employed for preparing the pentamethine cyanine dye analogues and a glutaconic aldehyde for preparing heptamethine cyanine dye analogues.
- the reaction is usually carried out in an organic solvent, such as pyridine and heated to reflux.
- the mixture subsequently is cooled and poured into an organic solvent such as ether.
- the resulting solid or semi-solid may be purified by chromatography on a silica gel column using a series of methanol/chloroform solvents.
- Unsymmetrical compounds of formula (I) wherein structures (A) and (B) are different may be conveniently prepared in a two step process.
- an intermediate compound is first formed by reacting an indolinium compound of formula (A) with a compound suitable for forming the linkage, for example, a suitably substituted N.N'-diphenylformamidine, or malonaldehyde dianil, in the presence of acetic anhydride, to form a 2-anilinovinyl or 4-anilino- 1 ,3-butadienyl quaternary salt.
- the intermediate quaternary salt may be reacted with a second 2-methyl indolinium quaternary salt to give a compound of formula (I).
- Groups R 1 , R 2 , R 3 , R 4 , R 5 , R 6 and R 7 may be chosen so that the dyes of the present invention have different wavelength characteristics, thereby providing a number of related dyes which can be used in multiparameter analyses wherein the presence and quantity of different compounds in a single sample may be differentiated based on the wavelengths of a number of detected fluorescence emissions.
- CyTM is a trademark of Amersham Biosciences UK Limited.
- Figure 1 are plots showing dye/protein ratio versus amount of applied NHS ester for Compound 2 and Compound 5.
- Figure 2 is plot of relative fluorescence intensity versus dye/protein ratio at constant antibody concentration for pentamethine cyanine dyes.
- Figure 3 shows the absorption spectra of IgG Conjugates of Compound 5 and
- Figure 4 shows the absorption spectra of IgG conjugates of Compound 6 compared with Compounds 3 and 4 at high-dye/protein ratios.
- Figure 5 is a plot showing relative fluorescence intensity versus dye/protein ratio for heptamethine cyanine dyes.
- Figure 6 is a spectral scan (200-700nm) of cDNA labelled with Compound 2.
- Figure 7 is a spectral scan (200-700nm) of cDNA labelled with Compound 5.
- Figure 8 is a spectral scan (200-700nm) of cDNA labelled with Compound 6.
- Figure 9 is a plot showing UV absorbance measured at intervals over 120 hours for Compounds 2, 5 and 6. Examples
- UVA is (Water+0.1%TFA): 269, 229nm
- the fractions containing the desired product were pooled and the solvent removed under reduced pressure.
- the product was obtained as a pale pink oil (400mg).
- Disodium 2,3-dimethyl-3-(4-sulphonatobutyl)-3 - -indole-5-sulphonate (1g), 6-bromohexanoic acid (3.2g, 16.41mmol) and tetramethylene sulphone (5ml) were heated together at 110°C under nitrogen for 14hrs. A further aliquot (3.2g, 16.41mmol) of bromohexanoic acid was then added and heating continued for 12hrs. A further aliquot (1.6g, 8.21mmol) of 6-bromohexanoic acid was then added and heating continued for a further 12hrs. The reaction mixture was cooled to RT and then poured into ethyl acetate.
- the product was filtered off, washed with ethyl acetate and then dried in vacuo at 40°C and obtained as a brown solid (2.71 g).
- UV/Vis (Water+0.1 %TFA): 653nm.
- Disodium 2,3-dimethyl-3-(4-sulphonatobutyl)-3H-indole-5-sulphonate (1.0g) and 1 ,4-butanesultone (10ml) were mixed and heated under nitrogen at 150°C for 52hrs to give a dark purple slurry. After cooling, the mixture was triturated with ethyl acetate: the solid portion was collected by filtration, washed with ethyl acetate and diethyl ether, then dried under high vacuum over phosphorus pentoxide to give the title product (1.45g), which was used directly without purification.
- Disodium 2,3-dimethyl-1 ,3-bis(4-sulphonatobutyl)-3H-indolium-5- sulphonate, crude (1.0g) and malonaldehyde bis(phenylimine) HCI (1.0g) and acetic acid (10ml) were heated under nitrogen at 130°C for 10hrs to give a dark orange-red solution.
- the solvent was then evaporated under vacuum; the residue was partitioned in a water / dichloromethane / methanol mixture.
- the aqueous layer was evaporated under vacuum and purified by HPLC (water/0.1 % TFA and acetonitrile/0.1% TFA eluants). Fractions containing the product were pooled and evaporated, freeze-dried from aqueous solution, with final drying under high vacuum over phosphorus pentoxide to give the title product. Yield 240mg as a red foam.
- UV/Vis Water+0.1 %TFA: 520nm.
- Fractions containing the desired product were pooled and the solvent removed under reduced pressure. The product was obtained as a dark green solid (7mg).
- LC-MS (ES + ): found 927. MH + C41H.54N 2 O14S4 requires 927. UV ⁇ /is; ⁇ max 754nm (PBS buffer).
- the fractions containing the desired product were pooled and the solvent removed under reduced pressure, the residue was then freeze dried.
- the product was obtained as a rust brown solid (4.14g).
- the dye was dissolved in water (7.5ml), filtered and purified by HPLC (Dynamax C ⁇ 8 42mm x 25cm) using water (0.1 % TFA) modified with a 20 to 30% acetonitrile (0.1 %TFA) gradient over 60 minutes. The flow rate was 20ml/min. Fractions containing desired product were combined and rotary evaporated to a small volume, transferred to a small bottle and freeze-dried o/n. UV ⁇ /is detection was at 650nm. Yield: 132mg. The partially purified material was then dissolved in water (7.5ml), filtered and purified by HPLC
- Sheep IgG was dissolved in sodium carbonate buffer (0.1 M, pH 9.2) at 1 mg/ml; the dye NHS esters were dissolved in anhydrous DMSO at ⁇ 10mg/ml (250 ⁇ l).
- a series of labelling experiments was carried out. Each reaction used 500 ⁇ l of antibody solution, combined with varying amounts of dye NHS ester solution, ranging from 0.1- 32.0 ⁇ l.
- the labelling reactions were rolled in the dark at ambient temperature for 45minut.es. Free dye was removed from the conjugates by purification by size exclusion chromatography using Sephadex as the stationary phase and phosphate buffered saline (PBS) of pH 7.4 as the eluant.
- PBS phosphate buffered saline
- Absorbance spectra were first measured on the neat conjugate solutions: in cases where the dye absorbance exceeded the linear range of the instrument ( ⁇ 1.5AU), a more dilute sample was made up using PBS and the readings scaled appropriately. Absorbance values were recorded at the dye absorption peak ( ⁇ 650nm) and at the antibody absorbance (280nm).
- Dye/protein ratios were calculated using the standard formula: A max .
- SAB D / P (A 28 o - x ⁇ A max ) . ⁇ D
- a 280 the absorbance at 280nm (the absorbance peak of the antibody)
- ⁇ AB the extinction coefficient of the antibody at 280nm (determined by experiment to be 170,000 dm 3 mol "1 cm “1 ).
- the conjugate solutions were diluted with PBS (200 ⁇ l conjugate into 20ml) and the fluorescence reading determined on a Perkin Elmer LS-55 instrument. Excitation was at the dye peak absorbance wavelength; emission at 680nm was recorded. Initial fluorescence readings were processed to take account of the actual concentration of antibody in each sample, as determined from the absorbance data. The readings for both sets of conjugates were thus scaled to a constant concentration of antibody; relative fluorescence was then plotted versus dye/protein ratio: Figure 2.
- Sheep IgG was dissolved in sodium carbonate buffer (0.1 M, pH 9.2) at 1 mg/ml; the dye NHS esters were dissolved in anhydrous DMSO at -1 Omg/ml (250 ⁇ l).
- a series of labelling experiments was carried out. Each reaction used 500 ⁇ l of antibody solution, combined with varying amounts of dye NHS ester solution, ranging from 0.5- 16 ⁇ l.
- the labelling reactions were rolled in the dark at ambient temperature for 45minutes. Free dye was removed from the conjugates by purification by size exclusion chromatography using Sephadex as the stationary phase and phosphate buffered saline (PBS) of pH 7.4 as the eluant.
- PBS phosphate buffered saline
- Absorbance spectra were first measured on the neat conjugate solutions; in cases where the dye absorbance exceeded the linear range of the instrument ( ⁇ 1.5AU), a more dilute sample was made up using PBS and the readings scaled appropriately. Absorbance values were recorded at the dye absorption peak ( ⁇ 750nm) and at the antibody absorbance (280nm).
- Dye/protein ratios were calculated using the standard formula given in Example 6; ⁇ o was taken as 250,000 dm 3 mol “1 cm “1 and x as 0.04. As seen in Figure 4, UV ⁇ /is absorbance of the conjugates in PBS shows
- UV ⁇ /is solutions were diluted further with PBS buffer in order to measure the relative fluorescence of the different heptamethine cyanine dye- labelled conjugates. Relative fluorescence was measured as described in Example 6.3 and then plotted versus dye/protein ratio: Figure 5. The results indicate that the IgG conjugates with Compounds 3 and 4 are brighter at higher loadings of dye than the standard Cy7 6-lgG conjugates.
- a sample (25 ⁇ l in 500 ⁇ l buffer) was analysed using analytical HPLC (Phenomenex Jupiter C ⁇ 8 10 ⁇ 25x0.46cm) in phosphate buffer 0.05M pH 5.6 modified with a 5 to 30% acetonitrile gradient over 30 minutes and constant flow rate of 1 ml/minute.
- Sample detection was made using absorbance at 254nm and 650nm.
- the chromatogram shows consumption of ester and amine to give a new product with a RT of 15.5 minutes.
- the product was diluted with water (1ml) and purified by ion-exchange chromatography (HiTrapQ HP 5ml) in 0.1 M triethyl ammonium bicarbonate buffer modified with 20 - 75% 1 M triethyl ammonium carbonate buffer over 60 minutes.
- the flow rate was 1 ml/min and detection was at 650nm and 254nm. Fractions corresponding to the major peak were combined and rotary evaporated with heat, dissolved in water, and freeze-dried.
- cDNA Probe labelling cDNA was labelled using Compounds 2 and 5 by means of a post- labelling technique in which the reactive NHS ester derivatives of the dyes were coupled to cDNA to generate microarray probes as described below.
- Purified human skeletal muscle messenger RNA (1 ⁇ g) was converted into cDNA using oligo-dT and random primers in the presence of aminoallyl- deoxyUTP, deoxynucleotides, reverse transcriptase and reaction buffer for 1hour at 42°C in 20 ⁇ l reactions following standard protocol outlined in the CyScribe Post Labelling Kit (GE Healthcare). Unincorporated nucleotides and buffers were removed from synthesized cDNA by binding cDNA on a glass- bead matrix. The aminoallyl-cDNA was eluted in water.
- the eluted cDNA was dried down and resuspended in aliquots of 0.1 M sodium bicarbonate buffer, pH 8.5 (40 ⁇ l) and separate aliquots mixed with reactive NHS esters of Compounds 2 and 5. Equal amounts of cDNA were used with 100-500 ⁇ g of Compounds 2 and 5 as their reactive NHS esters. The coupling reaction was carried out in the dark for 1 hr 30 minutes, followed by purification of labelled cDNA from un-reacted ester using a glass bead matrix. For comparison purposes, an aliquot of the cDNA was also labelled with Compound 6 (Cy5 NHS ester). The cDNA labelled probes were purified and the yields determined as described below. i) Calculation of Yield of Labelled cDNA
- Abs max the absorbance at the dye peak wavelength (650nm).
- ⁇ the extinction coefficient of the dye at the dye peak wavelength (250,000 mol "1 cm “1 ).
Abstract
Description
Claims
Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP04791610A EP1678258B1 (en) | 2003-10-31 | 2004-10-29 | Cyanine dye labelling reagents |
US10/576,956 US7750163B2 (en) | 2003-10-31 | 2004-10-29 | Cyanine dye labelling reagents |
JP2006537422A JP4943156B2 (en) | 2003-10-31 | 2004-10-29 | Cyanine dye labeling reagent |
AT04791610T ATE522580T1 (en) | 2003-10-31 | 2004-10-29 | CYANINE DYES AS MARKING AGENTS |
HK07100361.4A HK1093520A1 (en) | 2003-10-31 | 2007-01-10 | Cyanine dye labelling reagents |
US12/828,391 US8148539B2 (en) | 2003-10-31 | 2010-07-01 | Cyanine dye labelling reagents |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US51642803P | 2003-10-31 | 2003-10-31 | |
US60/516,428 | 2003-10-31 |
Related Child Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US10/576,956 A-371-Of-International US7750163B2 (en) | 2003-10-31 | 2004-10-29 | Cyanine dye labelling reagents |
US12/828,391 Division US8148539B2 (en) | 2003-10-31 | 2010-07-01 | Cyanine dye labelling reagents |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2005044923A1 true WO2005044923A1 (en) | 2005-05-19 |
Family
ID=34572883
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/GB2004/004573 WO2005044923A1 (en) | 2003-10-31 | 2004-10-29 | Cyanine dye labelling reagents |
Country Status (8)
Country | Link |
---|---|
US (2) | US7750163B2 (en) |
EP (2) | EP2360211A1 (en) |
JP (1) | JP4943156B2 (en) |
CN (1) | CN100577742C (en) |
AT (1) | ATE522580T1 (en) |
ES (1) | ES2372395T3 (en) |
HK (1) | HK1093520A1 (en) |
WO (1) | WO2005044923A1 (en) |
Cited By (30)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1792949A2 (en) | 2005-12-05 | 2007-06-06 | Dyomics GmbH | Hydrophilic marker on the basis of diastereomeric cyanines |
DE102006029454A1 (en) * | 2005-12-05 | 2007-06-06 | Dyomics Gmbh | Hydrophilic markers based on diastereomeric |
WO2008139206A2 (en) * | 2007-05-16 | 2008-11-20 | Ge Healthcare As | Optical imaging agents |
WO2007100711A3 (en) * | 2006-02-24 | 2009-04-09 | Investigen Inc | Methods and compositions for detecting polynucleotides |
JP2009527629A (en) * | 2006-02-21 | 2009-07-30 | エポック バイオサイエンシズ インコーポレーティッド | Phosphonylated fluorescent dyes and complexes |
EP2184326A2 (en) | 2008-09-17 | 2010-05-12 | Thermo Fischer (Milwaukee) LLC | Hydrophilic labels for biomolecules |
US7745119B2 (en) | 2003-05-20 | 2010-06-29 | Investigen, Inc. | System for detecting polynucleotides |
US7771947B2 (en) | 2007-02-23 | 2010-08-10 | Investigen, Inc. | Methods and compositions for rapid light-activated isolation and detection of analytes |
GB2478359A (en) * | 2010-03-05 | 2011-09-07 | Innova Biosciences Ltd | Reagents and methods relating to conjugation reactions |
WO2012001050A1 (en) | 2010-06-29 | 2012-01-05 | Ge Healthcare As | Dye compositions and dye syntheses |
WO2012001063A1 (en) | 2010-06-29 | 2012-01-05 | Ge Healthcare As | Dye compositions and dye syntheses |
WO2012048063A2 (en) * | 2010-10-06 | 2012-04-12 | Emd Millipore Corporation | Cyanine compounds, conjugates and method of use |
WO2012088007A1 (en) | 2010-12-21 | 2012-06-28 | Pierce Biotechnology, Inc. | Fluorescent compounds |
RU2475266C2 (en) * | 2007-05-16 | 2013-02-20 | ДжиИ Хелткер АС | Optical agents of visualisation |
DE102012107475A1 (en) | 2011-08-16 | 2013-02-21 | Dyomics Gmbh | BENZOCYANINVERBINDUNGEN |
US8389745B2 (en) | 2004-08-13 | 2013-03-05 | Elitech Holding B.V. | Phosphonate fluorescent dyes and conjugates |
WO2013041117A1 (en) | 2011-09-23 | 2013-03-28 | Illumina Cambrigde Ltd. | Dyes for labelling molecular ligands |
WO2013130761A1 (en) | 2012-03-02 | 2013-09-06 | Pierce Biotechnology, Inc. | Indole derivatives as labeling dye for biomolecule |
US8809551B1 (en) | 2013-03-08 | 2014-08-19 | Illumina Cambridge Limited | Polymethine compounds and their use as fluorescent labels |
US8877892B2 (en) | 2011-03-15 | 2014-11-04 | Innova Biosciences Limited | Conjugation reactions |
US8889884B1 (en) | 2011-07-14 | 2014-11-18 | Pierce Biotechnology, Inc. | Phosphine derivatives of fluorescent compounds |
US9309409B2 (en) | 2014-05-07 | 2016-04-12 | Illumina Cambridge Limited | Polymethine compounds and their use as fluorescent labels |
US20160176819A1 (en) * | 2014-12-19 | 2016-06-23 | Washington University | Asymmetrically charged nir fluorophores |
EP2618849A4 (en) * | 2010-09-20 | 2016-06-29 | Caliper Life Sciences Inc | Multivalent fluorescent probes |
US9676787B2 (en) | 2012-08-28 | 2017-06-13 | Pierce Biotechnology, Inc. | Benzopyrylium compounds |
US9751868B2 (en) | 2012-02-28 | 2017-09-05 | Pierce Biotechnology, Inc. | Benzocyanine compounds |
US10239909B2 (en) | 2015-05-22 | 2019-03-26 | Illumina Cambridge Limited | Polymethine compounds with long stokes shifts and their use as fluorescent labels |
US10982261B2 (en) | 2015-09-25 | 2021-04-20 | Illumina Cambridge Limited | Polymethine compounds and their use as fluorescent labels |
WO2021162133A1 (en) * | 2020-02-10 | 2021-08-19 | (주)씨바이오멕스 | Amphoteric fluorescent substance capable of being attached to biomaterials |
US11813338B2 (en) * | 2013-03-12 | 2023-11-14 | The Trustees Of The University Of Pennsylvania | Diagnosing and treating cancer |
Families Citing this family (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7750163B2 (en) * | 2003-10-31 | 2010-07-06 | GE Healthcare U.K. Limited | Cyanine dye labelling reagents |
CN101723874B (en) * | 2008-10-31 | 2013-09-11 | 深圳迈瑞生物医疗电子股份有限公司 | Cyanine compound and application thereof in dyeing biological samples |
CN102272106A (en) | 2008-11-07 | 2011-12-07 | 通用医疗公司 | Monofunctional carbocyanine dyes for in vivo and in vitro imaging |
CN101750476B (en) * | 2008-12-08 | 2015-06-03 | 深圳迈瑞生物医疗电子股份有限公司 | Blood analysis reagent and use method thereof |
US8729276B2 (en) * | 2009-04-17 | 2014-05-20 | Korea Institute Of Science And Technology | Cyanine compound for labeling biomolecule and preparation method thereof |
US20100291706A1 (en) * | 2009-05-15 | 2010-11-18 | Millipore Corporation | Dye conjugates and methods of use |
WO2013109859A1 (en) * | 2012-01-20 | 2013-07-25 | Cornell University | Dye compositions, methods of preparation, conjugates thereof, and methods of use |
CN102608051B (en) * | 2012-02-21 | 2015-04-08 | 中国科学院化学研究所 | Reagent kit for diagnosis of leukemia |
KR101953819B1 (en) * | 2017-12-22 | 2019-03-06 | (주)바이오액츠 | Novel cyanine compound for labeling biomolecule and preparation method thereof |
CN108752406A (en) * | 2018-05-29 | 2018-11-06 | 苏州百源基因技术有限公司 | A kind of nucleotide of fluorescent marker and its preparation method and application |
JP7359869B2 (en) * | 2019-12-19 | 2023-10-11 | 富士フイルム株式会社 | Compounds and fluorescently labeled biological substances using them |
US11964965B2 (en) | 2020-05-08 | 2024-04-23 | On Target Laboratories, LLC | Methods of manufacture and synthesis of fluorescent dye compounds and uses thereof |
CA3175996A1 (en) * | 2020-05-12 | 2021-11-18 | Julie KARPENKO | Fluorogenic dimer compound, useful as a probe for detection of endogenous receptors |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH04186342A (en) * | 1990-11-21 | 1992-07-03 | Fuji Photo Film Co Ltd | Preparation of color proof using photosensitive material for photograph |
JPH05313304A (en) * | 1992-05-11 | 1993-11-26 | Fuji Photo Film Co Ltd | Silver halide photographic sensitive material |
US5268486A (en) * | 1986-04-18 | 1993-12-07 | Carnegie-Mellon Unversity | Method for labeling and detecting materials employing arylsulfonate cyanine dyes |
WO2002026891A1 (en) * | 2000-09-29 | 2002-04-04 | Molecular Probes, Inc. | Modified carbocyanine dyes and their conjugates |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6048982A (en) | 1986-04-18 | 2000-04-11 | Carnegie Mellon University | Cyanine dyes as labeling reagents for detection of biological and other materials by luminescence methods |
US5569587A (en) | 1986-04-18 | 1996-10-29 | Carnegie Mellon University | Method for labeling and detecting materials employing luminescent arysulfonate cyanine dyes |
JPH0531330A (en) * | 1991-07-31 | 1993-02-09 | Riken Corp | Decontamination of exhaust gas |
DE4445065A1 (en) | 1994-12-07 | 1996-06-13 | Diagnostikforschung Inst | Methods for in-vivo diagnostics using NIR radiation |
US7615646B2 (en) * | 2002-05-10 | 2009-11-10 | Carnegie Mellon University | Chiral indole intermediates and their fluorescent cyanine dyes containing functional groups |
US7750163B2 (en) * | 2003-10-31 | 2010-07-06 | GE Healthcare U.K. Limited | Cyanine dye labelling reagents |
-
2004
- 2004-10-29 US US10/576,956 patent/US7750163B2/en not_active Expired - Fee Related
- 2004-10-29 EP EP11156595A patent/EP2360211A1/en not_active Withdrawn
- 2004-10-29 ES ES04791610T patent/ES2372395T3/en active Active
- 2004-10-29 AT AT04791610T patent/ATE522580T1/en not_active IP Right Cessation
- 2004-10-29 WO PCT/GB2004/004573 patent/WO2005044923A1/en active Application Filing
- 2004-10-29 EP EP04791610A patent/EP1678258B1/en not_active Not-in-force
- 2004-10-29 JP JP2006537422A patent/JP4943156B2/en not_active Expired - Fee Related
- 2004-10-29 CN CN200480032219A patent/CN100577742C/en not_active Expired - Fee Related
-
2007
- 2007-01-10 HK HK07100361.4A patent/HK1093520A1/en not_active IP Right Cessation
-
2010
- 2010-07-01 US US12/828,391 patent/US8148539B2/en not_active Expired - Fee Related
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5268486A (en) * | 1986-04-18 | 1993-12-07 | Carnegie-Mellon Unversity | Method for labeling and detecting materials employing arylsulfonate cyanine dyes |
JPH04186342A (en) * | 1990-11-21 | 1992-07-03 | Fuji Photo Film Co Ltd | Preparation of color proof using photosensitive material for photograph |
JPH05313304A (en) * | 1992-05-11 | 1993-11-26 | Fuji Photo Film Co Ltd | Silver halide photographic sensitive material |
WO2002026891A1 (en) * | 2000-09-29 | 2002-04-04 | Molecular Probes, Inc. | Modified carbocyanine dyes and their conjugates |
US20020077487A1 (en) * | 2000-09-29 | 2002-06-20 | Molecular Probes, Inc. | Modified carbocyanine dyes and their conjugates |
Non-Patent Citations (2)
Title |
---|
PATENT ABSTRACTS OF JAPAN vol. 016, no. 511 (P - 1441) 21 October 1992 (1992-10-21) * |
PATENT ABSTRACTS OF JAPAN vol. 018, no. 126 (P - 1702) 2 March 1994 (1994-03-02) * |
Cited By (58)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7745119B2 (en) | 2003-05-20 | 2010-06-29 | Investigen, Inc. | System for detecting polynucleotides |
US8389745B2 (en) | 2004-08-13 | 2013-03-05 | Elitech Holding B.V. | Phosphonate fluorescent dyes and conjugates |
DE102006029454A1 (en) * | 2005-12-05 | 2007-06-06 | Dyomics Gmbh | Hydrophilic markers based on diastereomeric |
EP1792949A2 (en) | 2005-12-05 | 2007-06-06 | Dyomics GmbH | Hydrophilic marker on the basis of diastereomeric cyanines |
US7745640B2 (en) | 2005-12-05 | 2010-06-29 | Dyomics Gmbh | Hydrophilic labels for biomolecules |
JP2009527629A (en) * | 2006-02-21 | 2009-07-30 | エポック バイオサイエンシズ インコーポレーティッド | Phosphonylated fluorescent dyes and complexes |
US8021839B2 (en) | 2006-02-24 | 2011-09-20 | Investigen, Inc. | Methods and compositions for detecting polynucleotides |
WO2007100711A3 (en) * | 2006-02-24 | 2009-04-09 | Investigen Inc | Methods and compositions for detecting polynucleotides |
US7771947B2 (en) | 2007-02-23 | 2010-08-10 | Investigen, Inc. | Methods and compositions for rapid light-activated isolation and detection of analytes |
RU2475266C2 (en) * | 2007-05-16 | 2013-02-20 | ДжиИ Хелткер АС | Optical agents of visualisation |
KR101578225B1 (en) * | 2007-05-16 | 2015-12-17 | 지이 헬스케어 에이에스 | Labelled HGF binding peptides for imaging |
WO2008139206A3 (en) * | 2007-05-16 | 2009-04-16 | Ge Healthcare As | Optical imaging agents |
WO2008139206A2 (en) * | 2007-05-16 | 2008-11-20 | Ge Healthcare As | Optical imaging agents |
US7951959B2 (en) | 2008-09-17 | 2011-05-31 | Thermo Fisher Scientific (Milwaukee) LLC | Hydrophilic labels for biomolecules |
EP2184326A2 (en) | 2008-09-17 | 2010-05-12 | Thermo Fischer (Milwaukee) LLC | Hydrophilic labels for biomolecules |
GB2478359B (en) * | 2010-03-05 | 2018-09-12 | Innova Biosciences Ltd | Conjugation reactions |
GB2478359A (en) * | 2010-03-05 | 2011-09-07 | Innova Biosciences Ltd | Reagents and methods relating to conjugation reactions |
WO2012001063A1 (en) | 2010-06-29 | 2012-01-05 | Ge Healthcare As | Dye compositions and dye syntheses |
WO2012001050A1 (en) | 2010-06-29 | 2012-01-05 | Ge Healthcare As | Dye compositions and dye syntheses |
EP2618849A4 (en) * | 2010-09-20 | 2016-06-29 | Caliper Life Sciences Inc | Multivalent fluorescent probes |
WO2012048063A2 (en) * | 2010-10-06 | 2012-04-12 | Emd Millipore Corporation | Cyanine compounds, conjugates and method of use |
WO2012048063A3 (en) * | 2010-10-06 | 2014-04-03 | Emd Millipore Corporation | Cyanine compounds, conjugates and method of use |
US10053447B2 (en) | 2010-12-21 | 2018-08-21 | Pierce Biotechnology, Inc | Fluorescent compounds |
EP3029027A1 (en) | 2010-12-21 | 2016-06-08 | Pierce Biotechnology, Inc. | Fluorescent compounds |
US10351551B2 (en) | 2010-12-21 | 2019-07-16 | Pierce Biotechnology, Inc. | Fluorescent compounds |
US11053222B2 (en) | 2010-12-21 | 2021-07-06 | Pierce Biotechnology, Inc. | Fluorescent compounds |
WO2012088007A1 (en) | 2010-12-21 | 2012-06-28 | Pierce Biotechnology, Inc. | Fluorescent compounds |
US8877892B2 (en) | 2011-03-15 | 2014-11-04 | Innova Biosciences Limited | Conjugation reactions |
US8889884B1 (en) | 2011-07-14 | 2014-11-18 | Pierce Biotechnology, Inc. | Phosphine derivatives of fluorescent compounds |
US9365598B2 (en) | 2011-07-14 | 2016-06-14 | Pierce Biotechnology, Inc. | Phosphine derivatives of fluorescent compounds |
US10125120B2 (en) | 2011-08-16 | 2018-11-13 | Pierce Biotechnology, Inc. | Benzocyanine compounds |
DE102012107475A1 (en) | 2011-08-16 | 2013-02-21 | Dyomics Gmbh | BENZOCYANINVERBINDUNGEN |
US9249307B2 (en) | 2011-08-16 | 2016-02-02 | Pierce Biotechnology, Inc. | Benzocyanine compounds |
DE102012107475B4 (en) | 2011-08-16 | 2024-02-08 | Dyomics Gmbh | BENZOCYANINE COMPOUNDS |
US10730857B2 (en) | 2011-08-16 | 2020-08-04 | Pierce Biotechnology, Inc. | Benzocyanine compounds |
US9376568B2 (en) | 2011-09-23 | 2016-06-28 | Illumina Cambridge Limited | Dyes for labelling molecular ligands |
WO2013041117A1 (en) | 2011-09-23 | 2013-03-28 | Illumina Cambrigde Ltd. | Dyes for labelling molecular ligands |
US9085698B2 (en) | 2011-09-23 | 2015-07-21 | Illumina Cambridge Limited | Dyes for labelling molecular ligands |
US9738786B2 (en) | 2011-09-23 | 2017-08-22 | Illumina Cambridge Limited | Dyes for labelling molecular ligands |
US10526317B2 (en) | 2012-02-28 | 2020-01-07 | Pierce Biotechnology, Inc. | Benzocyanine compounds |
US9751868B2 (en) | 2012-02-28 | 2017-09-05 | Pierce Biotechnology, Inc. | Benzocyanine compounds |
US10000467B2 (en) | 2012-03-02 | 2018-06-19 | Pierce Biotechnology, Inc. | Cyanine compounds |
EP3081564A1 (en) | 2012-03-02 | 2016-10-19 | Pierce Biotechnology, Inc. | Indole derivatives as labeling dye for biomolecule |
WO2013130761A1 (en) | 2012-03-02 | 2013-09-06 | Pierce Biotechnology, Inc. | Indole derivatives as labeling dye for biomolecule |
US10696653B2 (en) | 2012-03-02 | 2020-06-30 | Pierce Biotechnology, Inc. | Cyanine compounds |
US9676787B2 (en) | 2012-08-28 | 2017-06-13 | Pierce Biotechnology, Inc. | Benzopyrylium compounds |
US10174045B2 (en) | 2012-08-28 | 2019-01-08 | Pierce Biotechnology, Inc. | Benzopyrylium compounds |
US8809551B1 (en) | 2013-03-08 | 2014-08-19 | Illumina Cambridge Limited | Polymethine compounds and their use as fluorescent labels |
US9156987B2 (en) | 2013-03-08 | 2015-10-13 | Illumina Cambridge Limited | Polymethine compounds and their use as fluorescent labels |
US8993784B2 (en) | 2013-03-08 | 2015-03-31 | Illumina Cambridge Limited | Polymethine compounds and their use as fluorescent labels |
US11813338B2 (en) * | 2013-03-12 | 2023-11-14 | The Trustees Of The University Of Pennsylvania | Diagnosing and treating cancer |
US9309409B2 (en) | 2014-05-07 | 2016-04-12 | Illumina Cambridge Limited | Polymethine compounds and their use as fluorescent labels |
US10144967B2 (en) | 2014-05-07 | 2018-12-04 | Illumina Cambridge Limited | Polymethine compounds and their use as fluorescent labels |
US20160176819A1 (en) * | 2014-12-19 | 2016-06-23 | Washington University | Asymmetrically charged nir fluorophores |
US10239909B2 (en) | 2015-05-22 | 2019-03-26 | Illumina Cambridge Limited | Polymethine compounds with long stokes shifts and their use as fluorescent labels |
US10982261B2 (en) | 2015-09-25 | 2021-04-20 | Illumina Cambridge Limited | Polymethine compounds and their use as fluorescent labels |
US11530439B2 (en) | 2015-09-25 | 2022-12-20 | Illumina Cambridge Limited | Polymethine compounds and their use as fluorescent labels |
WO2021162133A1 (en) * | 2020-02-10 | 2021-08-19 | (주)씨바이오멕스 | Amphoteric fluorescent substance capable of being attached to biomaterials |
Also Published As
Publication number | Publication date |
---|---|
HK1093520A1 (en) | 2007-03-02 |
CN100577742C (en) | 2010-01-06 |
ES2372395T3 (en) | 2012-01-19 |
JP2007510031A (en) | 2007-04-19 |
CN1875073A (en) | 2006-12-06 |
JP4943156B2 (en) | 2012-05-30 |
ATE522580T1 (en) | 2011-09-15 |
US20070203343A1 (en) | 2007-08-30 |
EP1678258A1 (en) | 2006-07-12 |
EP1678258B1 (en) | 2011-08-31 |
US20100267937A1 (en) | 2010-10-21 |
EP2360211A1 (en) | 2011-08-24 |
US8148539B2 (en) | 2012-04-03 |
US7750163B2 (en) | 2010-07-06 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
EP1678258B1 (en) | Cyanine dye labelling reagents | |
EP1874871B1 (en) | Water soluble fluoro-substituted cyanine dyes as reactive fluorescence labelling reagents | |
US5453505A (en) | N-heteroaromatic ion and iminium ion substituted cyanine dyes for use as fluorescence labels | |
US7172907B2 (en) | Cyanine dye labelling reagents with meso-substitution | |
US8044203B2 (en) | Chiral indole intermediates and their fluorescent cyanine dyes containing functional groups | |
EP0769145A1 (en) | N-heteroaromatic ion and iminium ion substituted cyanine dyes for use as fluorescence labels | |
Markova et al. | Water soluble indodicarbocyanine dyes based on 2, 3-dimethyl-3-(4-sulfobutyl)-3H-indole-5-sulfonic acid | |
EP1654327A1 (en) | Cyanin-type compounds having an alkynyl linker arm | |
US7351829B2 (en) | Compounds on the basis of 2- and 4-chromenylidene-merocyanines respectively, and their use | |
EP1810998B1 (en) | Fluorescent cyanine dye | |
Zhan et al. | The synthesis and characterization of novel coumarin-containing cyanine dyes via “Click” chemistry | |
US6617458B2 (en) | Alpha cyano methine and polymethine dyes for the labeling of biological subtrates |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
WWE | Wipo information: entry into national phase |
Ref document number: 200480032219.4 Country of ref document: CN |
|
AK | Designated states |
Kind code of ref document: A1 Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BW BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE EG ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NA NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW |
|
AL | Designated countries for regional patents |
Kind code of ref document: A1 Designated state(s): GM KE LS MW MZ NA SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG |
|
121 | Ep: the epo has been informed by wipo that ep was designated in this application | ||
DPEN | Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed from 20040101) | ||
WWE | Wipo information: entry into national phase |
Ref document number: 2004791610 Country of ref document: EP Ref document number: 2176/DELNP/2006 Country of ref document: IN |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2006537422 Country of ref document: JP |
|
WWP | Wipo information: published in national office |
Ref document number: 2004791610 Country of ref document: EP |
|
WWE | Wipo information: entry into national phase |
Ref document number: 10576956 Country of ref document: US Ref document number: 2007203343 Country of ref document: US |
|
WWP | Wipo information: published in national office |
Ref document number: 10576956 Country of ref document: US |