WO2005034627A1

WO2005034627A1 - Pesticidal composition

Info

Publication number: WO2005034627A1
Application number: PCT/JP2004/015460
Authority: WO
Inventors: Shigeru Saito; Shinji Isayama
Original assignee: Sumitomo Chemical Company, Limited
Priority date: 2003-10-15
Filing date: 2004-10-13
Publication date: 2005-04-21
Also published as: TW200518676A; CN1893824A; TWI329001B; CN100577009C

Abstract

A pesticidal composition and so on characterized by containing 3,5-dichloro-1-(3,3-dichloro-2-propenyloxy)-4-[3-(5-trifluoromethylpyridin-2-yloxy)propyloxy]benzene and an insecticidal protein of Bacillus thuringiesis.

Description

Specification

Pest control composition

Technical field

The present invention relates to a pesticidal composition, more specifically, 3,5-dichloro-11- (3,3-dichloro-2-propenyloxy) -4- [3- (5-trifluoromethylpyridine). The present invention relates to a pesticidal composition containing (2-yloxy) propyloxy] benzene and an insecticidal protein of Bacillus thuringiesis. Background art

1,5-Dichrolic 1- (3,3-dichloro-2-propenyloxy) -4- [3- (5-trifluoromethylpyridine-2-yloxy) propyloxy] benzene may have insecticidal activity It is known (W096 / 1190.9).

Agriculture and horticulture using 3,5-dichloro-1- (3,3-dichroic-2--2-propenyloxy) -14- [3- (5_ (trifluoromethyl) pyridine-2-yloxy) propyloxy] benzene There is a demand for the development of pesticidal agents that exhibit excellent control effects at lower doses against pests. Disclosure of the invention

As a result of intensive studies under such circumstances, the present inventors have found that a pest control composition which can obtain a synergistic effect higher than the additive effect obtained when simply mixed, and which can reduce the amount of drug used. Were found, and the present invention was achieved.

That is, the present invention

1. 3,5-dichloro mouth—1- (3,3-dichloro-2-propenyloxy) —4-1

Insecticidal protein of [3- (5-trifluoromethylpyridine-1-2-yloxy) propyloxy] benzene (hereinafter sometimes referred to as the present compound) and Bacillus thuringiesis (hereinafter referred to as Bacillus thuringiesis) A pest control composition characterized by containing (hereinafter, referred to as the present composition). Sometimes. );

2. A synergistic effective amount of

3,5-dichloro mouth— 1— (3,3-dichloro-2-propinyloxy) -4-1- [3- (5-trifluoromethylpyridine-2-yloxy) propyloxy] benzene and Bacillus thuringiensis A pesticidal composition comprising: (Bacillus thuringiesis) an insecticidal protein;

3. 3,5-Dichloro-11- (3,3-dichloro-2-propinyloxy) -14- [3- (5-Trifluoromethylpyridine-12-yloxy) propyloxy] benzene and Bacillus thuringiensis (Bacillus thuringiesis), wherein the pesticidal protein is contained in a weight ratio of 1: 1000 to 10: 1, and the pesticidal composition according to the above 1 or 2;

4. If the insecticidal protein is a Bacillus thuringi esis culture, a viable cell, or a spore, the cell has been killed by heat or chemical means, or the cell of the cell The pesticidal composition according to the above 1 or 2, which is an insecticidal protein contained in the crushed product;

5. 3, .5-Dichloro_1- (3,3-dichloro-2-propenyloxy) -1-4_ [3- (5-trifluoromethylpyridine-12-yloxy) propyloxy] benzene and Bacillus chu A pest control method, which comprises applying an effective amount of both Bacillus thuringiesis and a pesticidal protein to a plant, a pest or a habitat of a pest to be protected;

6. a synergistic effective amount of

3,5-dichloro-1- (3,3-dichloro-2-propinyloxy)-4- [3- (5-trifluoromethylpyridine_2-yloxy) propyloxy] benzene and Bacillus'thuringiensis (Bacillus a pesticidal protein of S. thuringiesis) to the plant, pest or habitat of the pest to be protected;

7. 3,5-Dichro mouth—11 (3,3-Dichro mouth—2—Phenyloxy) for pest control 1. 4— [3— (5-Trifluoromethylpyridine 1-2) Iloxy ) Propoxy] benzene in combination with the insecticidal protein of Bacillus thur ingiesis;

8. A synergistic effective amount of 3,5-dichloro-1_ (3,3-dichloro-2-propidinyloxy) for pest control Methylpyridine_2-yloxy) propyloxy] benzene in combination with Bacillus thuringiesis insecticidal dampening protein;

And so on. BEST MODE FOR CARRYING OUT THE INVENTION

The compound used as one active ingredient in the composition of the present invention has the following formula (1)

It is a compound having insecticidal activity represented by (1). This compound is described in W096 / 11909, and can be produced by the method described in the publication. The present protein used as another active ingredient in the composition of the present invention is not particularly limited as long as it is an insecticidal protein of Bacillus thuringiesis.Specifically, for example, CryIA (a ), CryIA (b), CrylA (c), CryIB, CryIC, CryID, CryIF, CryIIA, CryllB, CryIIIA, CrylllB, Crylll CrylllD, CryV, CryVI and the like.

These proteins are naturally produced by bacteria belonging to Bacillus th uringiesis. Among them, for example, among them, for example, kurstaki, aisawai, tenebrionis (tenebrionis), japonensis-buibu ( japonensis Buibui). It is preferably used in the composition of the present invention.

In addition, this protein may be produced by a bacterium belonging to Bacillus thuringiesis which exists in nature, and may be a transformant produced by a conventional genetic engineering technique. (Eg, Escherichia coli, Bacillus subtilis, plants, etc.). As a gene having a nucleotide sequence encoding the amino acid sequence of the present protein (hereinafter sometimes referred to as the present gene) used for producing such a transformant, (1) naturally occurring It may be a gene that has been cloned from existing genes, or (2) deletion, substitution or partial deletion of some bases in the base sequence of a gene that has been cloned from naturally occurring genes. It may be a gene into which the addition is artificially introduced [that is, a gene obtained by subjecting a naturally-occurring gene to a mutation treatment (partial mutation introduction treatment, mutation treatment, etc.)], or It may be a gene that has been synthetically synthesized. The microorganisms as described above may be isolated from nature or purchased from a strain preservation organization, etc., and artificially purified from such microorganisms according to ordinary microbiological or genetic engineering techniques. It may be produced in a special way.

When separating from nature, for example, first, soil is collected from the field. After suspending the collected soil in sterile water, the obtained suspension is applied to a solid medium used for separating microorganisms such as Bacillus subtilis, for example, and cultured at 25 ° C. A few days later, an independent colony of the growing bacteria is cut out, transplanted to a solid medium used for separating microorganisms such as Bacillus subtilis, and further cultured at 25 ° C. About the bacteria that have grown, SNEATH, (PHA), MAIR, (NS) SHARPE, (ME) and HOLT, (JG): Bergey's manual of Systematic Bacteriology. Vol.2.1984, Williams and Wilkins, Baltimore. Bacteria belonging to Bacillus thuringiesis may be selected by identifying whether the bacterium belongs to Bacillus thuringies is in accordance with the method described in the above.

Next, for the selected bacteria belonging to Bacillus thuringiesis, the presence or absence of insecticidal proteins in the bacteria is determined by, for example, biological activity. Bacteria belonging to Bacillus thuringiensis may be selected by confirming according to an evaluation method, a microscopic observation method, or the like. The present protein used in the composition of the present invention may be the protein itself as it is, for example, a culture of Bacillus thuringiesis, a viable cell, a spore, or a bacterium that is killed by heat or chemical means. It may be a fungus treated, or a pesticidal protein contained in a crushed product of the fungus.

As a medium for culturing a bacterium belonging to Bacillus thuringiesis that produces an insecticidal protein, a general bacterium culture medium, a normal bouillon liquid medium, and the like can be mentioned. Any medium can be used. As described above, a culture containing bacteria belonging to Bacillus thuringiesis and a product thereof, which has been cultured using the above-described medium, is centrifuged and / or dried together with the medium, and the present invention is performed. It can be used as the present protein, which is the other active ingredient of the composition. Alternatively, the insecticidal protein itself isolated from the culture according to a known method may be used as it is as the present protein. Incidentally, the molecular weight of this protein is generally tens of thousands to several hundred thousand daltons. This protein is released together with spores in the form of crystalline material when bacteria belonging to Bacillus thuringiesis that have undergone a predetermined number of divisions are disrupted.

As the present protein used in the composition of the present invention, a commercially available BT preparation may be used, and preferably, Dipole (registered trademark) (Sumitomo Chemical Co., Ltd.), Esmarck (registered trademark) (Sumitomo) Chemical Industry Co., Ltd.), Flowpack (registered trademark) (Sumitomo Chemical Co., Ltd.), Zentari (registered trademark) (Sumika Takeda Agrochemical Co., Ltd.), Novodor (registered trademark) (Valent Biosciences co.), Buoy Hunter (registered trademark) (Sumitomo Chemical Co., Ltd .: Business transferred from Kupo Yu Biotech on December 12, 2003), Design (registered trademark) (Thermo Trilogy co.), Etc. The composition of the present invention can be used for controlling pests (for example, insects and harmful mites) in a wide range of agricultural and horticultural industries. The following pests are typical examples. Hemiptera: Insects such as the brown beetle (Laodelphax striatellus), the brown beetle (Nil aparvata lugens), the stag beetle (Sogatella furcifera), the black-tailed tuna 3n / ^ f (Nep otettix cincticeps), and the cyca midea Aphids such as leafhoppers, Aphis gossypii, Aphid aphids (Myzus persicae), and aphids, Lippaphis pserudobrassicae; stink bugs; Bemisia tabaci), whiteflies such as Bemisia argent ifo Hi, beetles, dung beetles, psyllids, etc.

Lepidopteran insect pests: Chilo suppressalis, Cnaphalocroci s medinalis, Ostrinia nubilalis, Parapediasia teierrella, etc., and Spodoptera litura. Toto (Spodoptera exigua), Atoto (Pseudaletia separata), Totoga (Mamestra brasicae), Tamanaya sword (Agrotis ipsilon), Iraksaginjiba (Trichoplusia ni), Tamanaginpa (Autographa nigrisigna sp.) , Helicoveira spp., Airis spp., Etc., White butterfly, Pieris rapae crucivora, White butterfly, Adoxophyes onmai, Apple cocoa Kumonhamaju (Adoxophyes orana fasciata), Nashihimenshi (Grapholita mo lest a), Codling Moss (Cy dia pomonella), toads such as peach moth (Carposina ni ponensis), etc .;

(Phyllocnisiis citrella) and other moth moths, stags (Plutella xylosiella) and other stags, squirrels (Euproctis taiwana), gypsy moths (Lymantria dispar), and skulls and other squashes (Euproctis similis) Irrigans, Calleha moths such as McDoreja (Dendrolimus spectabilis), Kibaga moths such as pink ball worms (Pectinophora gossypiella), hitriggers, japonic moths, etc.

Diptera pests: Ligustrum flies (Liriomyza trifolii), Nasal flies (Lir) iomyza bryoniae), tomato nomomoglino ^ e (Li riomyza sat ivae), omenasuji hamogrinoe (Liriomyza as terivora), namoglieno (Chromatomyia hort icol a), etc. , Anopheles, Mussidae, Musca domestica, Black flies, Drosophila, Anopheles, Thysanidae, Fruit flies, Drosophila, Drosophila, Aphids, Bubu, Dwarfs

Coleopteran pests: beetles, scarab beetles, weevils, beetles, ladybirds, beetles, beetles, etc.

Thrips of the order Thrips palmi: Thrips palmi, etc., genus Frankliniella, such as Franklin iniel la occidental is, Sci l tothrips dorsal is And others of the genus Siltthrips, such as

Hymenopteran pests: wasps, squirrels, wasps, etc.

Reticulate pests: cockroaches, cockroaches, etc.

Orthoptera: Pests, Kera

Pests of the order Coleoptera: Wild flea

Pests of the lice: lice

Termite pests: termites such as the termite (Ret icul i termes speratus) and the termite (Coptotermes formosanus)

Acarid pests: spider mites such as Tetranychus spp. And Panonychus spp., Fucus mites such as Tomato rust mite (Aculops lycopersic i), mites and ticks. Uses of the composition of the present invention are not particularly limited, but include cabbage, Chinese cabbage, Chinese cabbage, Japanese radish, broccoli, lettuce, spinach, tomato, eggplant, bell pepper, capsicum, kiyuri, watermelon, melon, asparagus. , Green onions, strawberries, bananas, rice, rye, wheat, barley, oats, amber, sweet potatoes, potatoes, corn, green beans, field beans, soybeans, red beans, sugar beet, sugar cane, tea, tabaco , Forage, beet edible root, cacao, carrot, kyasapa, celery, chickpea, citrus Various kinds of fruits such as tachibana, coconut, coffee, cotton, fig, grape, apple, apple, pear, peach, plum, oyster, guava, okra, ornamental plant, papaya, peanut, pepper, pigeon, pineapple, etc. It can be used to control pests for a variety of agricultural and horticultural applications on different plants and fruits.

Also, for example, cultivated flowers such as lawn, carnation, cherry, rose tree, gerbera, chrysanthemum, potted plant, and cactus can be protected by the present invention. Examples of cultivated vegetation would include all ornamental and flowering shrubs. In the composition of the present invention, the mixing ratio of the present compound and the present protein may be any mixing ratio that provides a synergistic effect. For example, a weight ratio of 1: 10000 to 10000: 1 may be used. Ratio, preferably 1: 100 to 100: 1 ratio, more preferably 1: 100 to 5: 1 ratio, more preferably 1:40 to 1: 1 ratio, especially Preferably, the ratio of 1:20 to 1: 1 can be used.

When the composition of the present invention is used, the present compound and the present protein may be used as a mixture without adding any other component, but usually, the mixture is further mixed with a solid carrier, a liquid carrier, Oils, emulsions, water-dispersible powders, water-dispersible granules, suspensions in water It is preferably used as various preparations such as powders, granules, aerosols, microcapsules, heat fumigants, poison baits and the like. These preparations usually contain the composition of the present invention as an active ingredient in an amount of about 0.01 to 95% by weight. Examples of the solid carrier used in the formulation include clays (kaolin clay, diatomaceous earth, bentonite, fubasami clay, acid clay, etc.), synthetic hydrous silicon oxide, talc, ceramics, other inorganic minerals ( Fine powders and granular materials such as sericite, quartz, sulfur, activated carbon, calcium carbonate, hydrated silica, etc., and chemical fertilizers (ammonium sulfate, phosphorous ammonium, ammonium nitrate, urea, salt ammonium, etc.).

Examples of the liquid carrier include water, alcohols (methanol, ethanol, etc.), ketones (acetone, methyl ethyl ketone, etc.), and aromatic hydrocarbons (toluene, xylene, etc.). , Ethylbenzene, methylnaphthalene, etc.), non-aromatic hydrocarbons (hexane, cyclohexane, kerosene, gas oil, etc.), esters (ethyl acetate, butyl acetate, etc.), nitriles (acetonitrile, isoptyronitrile) ), Ethers (diisopropyl ether, dioxane, etc.), acid amides (N, N-dimethylformamide, N, N-dimethylacetoamides), halogenated hydrocarbons (dichloromethane, trichloroethane, carbon tetrachloride, etc.) ), Dimethylsulfoxide, vegetable oils (soybean oil, cottonseed oil, etc.).

Examples of the gaseous carrier, that is, the propellant include, for example, CFC gas, butane gas, liquefied petroleum gas, dimethyl ether, and carbon dioxide gas.

Examples of surfactants include alkyl sulfates, alkyl sulfonates, alkyl aryl sulfonates, alkyl aryl ethers and polyoxyethylene compounds, polyethylene glycol ethers, polyhydric alcohol esters, and sugars. Alcohol derivatives and the like.

Other pharmaceutical auxiliaries include, for example, casein, gelatin, sugars (starch, arabia gum, cellulose derivatives, alginic acid, etc.), lignin derivatives, bentonite, synthetic water-soluble polymers (polyvinyl alcohol, polyvinylpyrrolidone, Acrylic acid, etc.), PAP (isopropyl isopropyl phosphate), BHT (2,6-di-tert-butyl-4 monomethylphenol), BHA (2-teri-butyl-4-methoxyphenol and 3-tert-butyl-4) -Methoxyphenol), vegetable oils, mineral oils, surfactants, fatty acids, fatty acid esters and the like.

Base materials for poison bait include, for example, bait ingredients such as cereal flour, vegetable oil, sugar, and crystalline cellulose; antioxidants such as dibutylhydroxytoluene and nordihydroguaiaretic acid; preservatives such as dehydroacetic acid; and pepper powder. And inducing flavors such as cheese flavor, evening onion flavor and the like. Suspension in water · Floables such as emulsions in water usually contain about 1 to 75% by weight of the active ingredient compound and about 0.5 to 15% by weight of a suspending aid (for example, protective colloid or thixotrope). About 0 to 10% by weight of auxiliary agents (eg, antifoaming agent, anti-foaming agent, stable) Agents, spreading agents, penetration aids, antifreezing agents, preservatives, antifungal agents, etc.).

The composition of the present invention can also be prepared by formulating each active ingredient by the above-mentioned formulation technique, and then mixing these formulations. That is, the composition of the present invention may be prepared by mixing a composition prepared in advance with the present compound and a composition prepared in advance with the present protein, depending on the form of the preparation. Also, both can be mixed or used at the time of application.

The composition of the present invention thus formulated is used as it is or after being diluted with water or the like. It is also possible to mix or use other insecticides, acaricides, nematicides, fungicides, herbicides, plant growth regulators, synergists, fertilizers, soil conditioners, animal feeds, etc. it can. The composition of the present invention is applied to pests, habitats of pests, plants to be protected from pests, and the like. At that time, when the composition of the present invention is formulated into an emulsion, a water-dispersible powder, a water-dispersible granule, a flowable or the like, the composition is usually diluted with water or the like before treatment.

When processing the composition of the present invention on pests in agricultural and horticultural, the effective amount or application amount of the present composition is generally about at 1 0 0 O m ² application rate per 0. L~1 0 0 0 g. When the active ingredient for controlling pests is applied in the form of granules, dusts, oils, etc., it is usually sufficient to apply the active ingredient as it is without any dilution so that the above effective amount or application amount is obtained. .

Even when the active ingredient for controlling pests is applied after diluting the emulsion, wettable powder, wettable granule, flowable, etc. in water, dilute it with water so that the above effective amount or application rate is achieved. And then apply. Specifically, for example, when an emulsion, a water-dispersible powder, a water-dispersible granule, a floor pull agent and the like are diluted with water and used, the application concentration of the composition of the present invention is usually about 1 to 100 0 ppm can be raised.

The above effective amount or application amount depends on the type of drug product, application period, application place, application method, type of pest, degree of damage, etc., and should be selected as appropriate by increasing or decreasing without affecting the above range. Can be. Example

Hereinafter, the present invention will be described in more detail with reference to Formulation Examples and Test Examples, but the present invention is not limited to these Examples.

The “BT culture” described below is defined as a spore suspension 100 1 containing 1 × 10 ³ viable spores per liter of a bacterium belonging to Bacillus thuringiesis, and 50 Om Inoculate a sterilized L-broth liquid medium (100 ml) in a 1-volume flask, shake culture (15 Orpm) for about 2 to 5 days at 25 ° C, and then obtain The spores derived from bacteria belonging to Bacillus thuringiesis and the insecticidal protein were recovered from the culture broth by centrifugation and dried to obtain a culture (ie, one of the present proteins). Form). Formulation Example 1 Emulsion

1 part of the present compound and 10 parts of a culture of BT bacteria were dissolved in 36.5 parts of xylene and 36.5 parts of dimethylformamide, and 10 parts of polyoxyethylenestyrylphenyl ether and 6 parts of calcium dodecylbenzenesulfonate were added thereto. And the mixture is mixed well to obtain an emulsion. Formulation Example 2 wettable powder

Add 5 parts of this compound and 45 parts of BT culture to a mixture of 4 parts of sodium lauryl sulfate, 2 parts of lignin calcium sulfonate, 22 parts of synthetic hydrous silicon oxide fine powder and 22 parts of diatomaceous earth, and mix well. To obtain a wettable powder. Formulation Example 3 Granules

To 1 part of this compound, 5 parts of a culture of BT bacteria, 5 parts of synthetic hydrous silicon oxide fine powder, 5 parts of sodium dodecyl benzene sulphonate, 30 parts of bentonite and 54 parts of clay are mixed well, and then mixed well. Add the amount of water, stir further, and make Granulate and air-dry to obtain granules. Formulation Example 4 Dust

After 0.5 part of this compound, 4 parts of BT bacterial culture, 1 part of synthetic hydrous silicon oxide fine powder, 1 part of Drilles B (manufactured by Sankyo) as a flocculant and 7 parts of clay were mixed well in a mortar. Stir and mix with a juice mixer. 86.5 parts of cut clay is added to the obtained mixture, and the mixture is sufficiently stirred and mixed to obtain a powder. Formulation Example 5 Flowable

1 part of the present compound, 10 parts of the BT bacteria culture and 1.5 parts of sorbitan trioleate were added to 26 parts of an aqueous solution containing 2 parts of polyvinyl alcohol, mixed well, and then the mixture was added. After finely pulverizing with a sand glider (particle size: 3 m or less), add 48 parts of an aqueous solution containing 0.05 part of xanthan gum and 0.1 part of aluminum magnesium silicate, and further add 15 parts of propylene glycol. In addition, the mixture is stirred and mixed to obtain a flooring agent. Formulation Example 6 Oil

One part of the present compound and 0.8 part of the BT bacterial culture are dissolved in 5 parts of xylene and 5 parts of trichloroethane, and mixed with 89.1 parts of deodorized kerosene to obtain an oil. Formulation Example 7 Microforce capsule

After mixing 2 parts of the present compound, 8 parts of BT bacterial culture, 10 parts of phenyloxylylethane and 0.5 part of Sumidur L-175 (Tolylene diisocyanate manufactured by Sumitomo Bayer Urethane Co., Ltd.) And 20 parts of a 10% aqueous solution of gum arabic, and stirred with a homomixer to obtain an emulsion having an average particle size of 20 m. Next, 2 parts of ethylene glycol is added thereto, and the mixture is further reacted for 24 hours in a warm bath at 60 ° C. to obtain a micro force capsule slurry. On the other hand, 0.2 part of Xanthan gum and 1.0 part of Vegum R (aluminum magnesium silicate manufactured by Sanyo Chemical Co., Ltd.) were dispersed in 56.3 parts of ion-exchanged water to increase the viscosity. To obtain an agent solution. 42.5 parts of the above micro force capsule slurry and 57.5 parts of the thickener solution are mixed to obtain microcapsules. Next, test examples show that the composition of the present invention exhibits an excellent pest control effect. The theoretical value of the insecticidal rate expected when two types of active ingredients are mixed and treated is E = X + Y one (XXY / 100) (By the way, this formula is calculated by the formula of Co 1 by This is an equation used to calculate criteria for synergistic and antagonistic effects on a combination of drugs that are generally called.) In the formula,

X: Insecticidal rate when active ingredient A is treated with Mpm

Y: Insecticidal rate when active ingredient B is treated with Nppm (%)

E: Expected theoretical value of insecticidal rate when active ingredient A is treated with Mp pm and active ingredient B with Np pm () Test Example 1 (artificial sample test against Heliothis virescens)

A 10% emulsion of this compound is mixed with an aqueous solution of BT bacteria preparation: Design® (Bacillus tlmr in giensis var. Aizawai strain GC91 (conjugant) Wettable granules preparation, manufactured by Thermo Trilogy Corporation) Thus, test solutions diluted to the respective predetermined concentrations were prepared. After injecting artificial feed (about 2 g) into the bottom of a plastic cup (30 ml capacity), 0.2 ml of the test solution was treated. After air-drying for about 2 hours after the treatment, one second-instar larva of the test insect (Heliothis virescens) was released (20 repetitions). Five days after the treatment, the mortality was calculated, and the mortality was corrected by the following formula to calculate the mortality (%).

Insecticidal rate (%) = 100 X (M t— Mc) / (100— Mc)

M t: Insect mortality rate in test compound treated area (%)

Mc: mortality rate in test compound-untreated area (%) table 1

Test Example 2 (Leaf dipping test on Spodoptera litura)

A 10% emulsion of this compound is mixed with a BT bacteria preparation: Flowback (registered trademark) (Bacillus thuringiensis var. Aizawai), a dry-flowable preparation, manufactured by Sumitomo Chemical Co., Ltd.) and diluted with water. A test solution was prepared by adding a spreading agent (special Reno-1: manufactured by Nippon Agrochemical Co., Ltd.) to the diluted solution diluted to a concentration of 1/5000 of the diluted solution. The cabbage leaves at the 5th to 8th leaf stage of the pot plant were cut out and immersed in the test solution for 60 seconds. In a plastic cup (500 ml capacity), filter paper with a diameter of 90 mm was laid, and on this was placed cabbage leaves that had been immersed and air-dried. Ten third-instar larvae of the test insect (Spodoptera litura) were released on the cabbage (three replicates). Six days after the treatment, the mortality of the test insects was determined, and the mortality was corrected by the following formula to calculate the mortality (%). The results are shown in Table 2.

Insecticidal rate (%) = 100 X (M t -Mc) / (100 -Mc)

Mt: Insect mortality rate in test compound treated area (%)

Mc: Death rate (%) in the test compound untreated area Table 2

Industrial potential

INDUSTRIAL APPLICABILITY The present invention can provide a pest control composition which can provide a synergistic effect higher than the additive effect obtained by simply mixing and can reduce the amount of drug used.

Claims

The scope of the claims

1. 3,5-dichloro-1- (3,3-dichloro-2-propenyloxy) 1-41

A pesticidal composition comprising [3- (5-trifluoromethylpyridine-1-2-yloxy) propyloxy] benzene and an insecticidal protein of Bacillus thuringiesis. .

2. a synergistic effective amount of

3,5-dichloro-11- (3,3-dichloro-1-propenyloxy) -1-4- [3- (5-trifluoromethylpyridine-12-yloxy) propyloxy] benzene and Bacillus thuringiensis (Bacillus and a pesticidal protein of P. thuringiesis).

3. 3,5-Dichro mouth—1_ (3,3-Dichro mouth—2-Prodenyloxy) 1-4— [3- (5-Trifluoromethylpyridine-12-yloxy) propyloxy] benzene and bacillus '' The pesticidal composition according to claim 1, wherein the pesticidal protein of Thuringiensis (Bacillus thuringiesis) is contained in a weight ratio of 1: 1000 to 10: 1.

4. If the insecticidal protein is a Bacillus thuringi esis culture, a viable cell, or a spore, the cell has been killed by heat or chemical means, or the cell of the cell The pesticidal composition according to claim 1 or 2, which is an insecticidal protein contained in the crushed product. 5. 3,

5-Dichro mouth-1-1 (3,3-dichloro-2_propenyloxy) -4

Plants that need to protect the effective amount of both [3- (5-trifluoromethylpyridine-2-yloxy) propyloxy] benzene and the insecticidal protein of Bacillus thuringiesis (Bacillus thuringiesis) , Pests or habitats of pests Pest control method characterized by applying to a place

6. a synergistic effective amount of

3,5-dichloro-1- (3,3-dichloro-2--1-propenyloxy) -4-1 [3- (5-trifluoromethylpyridine-2-yloxy) propyloxy] benzene and Bacillus thuringiensis (Bacillus thuringiesis) an insecticidal protein, which is applied to plants, pests or habitats of pests to be protected.

7. For control of pests, 3,5-dichroic mouth—1— (3,3-dichroic mouth—2-pulp benzoyloxy) 1-41 [3— (5—trifluoromethylpyridine—2— (Iloxy) Propoxy] Use of benzene in combination with Bacillus thur ingiesis insecticidal protein.

8. A synergistic effective amount of 3,5-dichloro-1 (3,3-diclo- mouth-2-propidinyloxy) for pest control —4— [3— (5— Trifluoromethylpyridine-1-2-yloxy) propyloxy] benzene in combination with Bacillus thuringiesis insecticidal protein.