CN100577009C - Vermin controlling composition - Google Patents
Vermin controlling composition Download PDFInfo
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- CN100577009C CN100577009C CN200480037191A CN200480037191A CN100577009C CN 100577009 C CN100577009 C CN 100577009C CN 200480037191 A CN200480037191 A CN 200480037191A CN 200480037191 A CN200480037191 A CN 200480037191A CN 100577009 C CN100577009 C CN 100577009C
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- chloro
- base
- oxygen base
- bacillus thuringiensis
- insecticidal proteins
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
- A01N63/23—B. thuringiensis
Abstract
A pesticidal composition and so on characterized by containing 3,5-dichloro-1-(3,3-dichloro-2-propenyloxy)-4-[3-(5-trifluoromethylpyridin-2-yloxy)propyloxy]benzene and an insecticidal protein of Bacillus thuringiesis.
Description
Technical field
The present invention relates to a kind of pest control composition, more specifically, relate to and a kind ofly comprise 3,5-two chloro-1-(3,3-two chloro-2-acrylic oxygen bases)-4-[3-(5-5-flumethiazine-2-base oxygen base) propyl group oxygen base] pest control composition of insecticidal proteins of benzene and bacillus thuringiensis (Bacillus thuringiesis).
Background technology
3,5-two chloro-1-(3,3-two chloro-2-acrylic oxygen bases)-4-[3-(5-5-flumethiazine-2-base oxygen base) propyl group oxygen base] to have insecticidal activity be known (international open text WO96/11909) to benzene.
A kind of pest control agent is developed in expectation, and it utilizes 3,5-two chloro-1-(3,3-two chloro-2-acrylic oxygen bases)-4-[3-(5-5-flumethiazine-2-base oxygen base) propyl group oxygen base] benzene just showing good control efficiency to agriculture and gardening pest insect than low dosage.
Detailed Description Of The Invention
In these cases, by research in earnest, the present inventor has found a kind of combination of pest control composition form, finished the present invention, this combination has obtained synergistic effect, is higher than adding and effect that simple mixing obtains, thereby has reduced using dosage.
That is to say, the invention provides:
1. a pest control composition (hereinafter being called the present composition sometimes), comprise 3,5-two chloro-1-(3,3-two chloro-2-acrylic oxygen bases)-4-[3-(5-5-flumethiazine-2-base oxygen base) propyl group oxygen base] insecticidal proteins (hereinafter being called albumen of the present invention sometimes) of benzene (hereinafter being called The compounds of this invention sometimes) and bacillus thuringiensis.
2. a pest control composition comprises 3 of synergy effective dose, 5-two chloro-1-(3,3-two chloro-2-acrylic oxygen bases)-4-[3-(5-5-flumethiazine-2-base oxygen base) propyl group oxygen base] insecticidal proteins of benzene and bacillus thuringiensis.
3. according to above-mentioned 1 or 2 pest control composition, wherein 3,5-two chloro-1-(3,3-two chloro-2-acrylic oxygen bases)-4-[3-(5-5-flumethiazine-2-base oxygen base) propyl group oxygen base] weight ratio of insecticidal proteins of benzene and bacillus thuringiensis is 1: 1000-10: 1.
4. according to above-mentioned 1 or 2 pest control composition, wherein said insecticidal proteins is that culture, living cells, spore, its heat or the chemistry of bacillus thuringiensis killed the insecticidal proteins that contains in form or its decomposed form.
5. insect pest control method; comprise to the habitat of claimed plant, insect or insect and use 3 of effective dose; 5-two chloro-1-(3,3-two chloro-2-acrylic oxygen bases)-4-[3-(5-5-flumethiazine-2-base oxygen base) propyl group oxygen base] combination of insecticidal proteins of benzene and bacillus thuringiensis.
6. insect pest control method; comprise to the habitat of claimed plant, insect or insect and use 3 of synergy effective dose; 5-two chloro-1-(3,3-two chloro-2-acrylic oxygen bases)-4-[3-(5-5-flumethiazine-2-base oxygen base) propyl group oxygen base] insecticidal proteins of benzene and bacillus thuringiensis.
7.3,5-two chloro-1-(3,3-two chloro-2-acrylic oxygen bases)-4-[3-(5-5-flumethiazine-2-base oxygen base) propyl group oxygen base] and the insecticidal proteins of benzene and bacillus thuringiensis is used for the combined use of control of insect; With
8. 3 of synergy effective dose, 5-two chloro-1-(3,3-two chloro-2-acrylic oxygen bases)-4-[3-(5-5-flumethiazine-2-base oxygen base) propyl group oxygen base] insecticidal proteins of benzene and bacillus thuringiensis is used for the combined use of control of insect.
Implement preferred plan of the present invention
The The compounds of this invention that is used as a kind of active component in the present invention is to have insecticidal activity, by the compound of following formula (1) expression.
The compounds of this invention is described among the international open text WO96/11909, and can prepare by the method described in the disclosure text.
The albumen of the present invention that is used as another kind of active component in the present invention is if the insecticidal proteins of bacillus thuringiensis, then have no particular limits, its concrete example comprises CryIA (a), CryIA (b), CryIA (c), CryIB, CryIC, CryID, CryIF, CryIIA, CryIIB, CryIIIA, CryIIIB, CryIIIC, CryIIID, CryV, CryVI etc.
Above-mentioned albumen of the present invention is that to belong to the bacterium of bacillus thuringiensis spontaneous; Wherein, be preferred for composition of the present invention by the above-mentioned bacteriogenic albumen of the present invention that belongs to Kustak subspecies (kurstaki), Aizawa subspecies (aizawai), plan walking first subspecies (tenebrionis) and Japanese subspecies (japonensis Buibui).
Albumen of the present invention can be prepared by the bacterium that belongs to naturally occurring bacillus thuringiensis, or by utilizing the conventional transformant that technique for gene engineering produced (as Escherichia coli (E.coli), bacillus subtilis (Bacillus subtilis) and plant) to prepare.The gene (hereinafter being called gene of the present invention sometimes) of the nucleotide sequence that comprises code book invention Argine Monohydrochloride sequence that uses in the above-mentioned transformant of preparation can be (1) gene by naturally occurring gene clone, (2) from the gene nucleotide series of naturally occurring gene clone, comprise the gene [just naturally occurring gene being carried out the gene of mutagenic treatment (site-directed mutagenesis and random mutagenesis)] of introducing disappearance, replacement or insertion portion nucleotide artificially, or the gene of (3) synthetic.
Above-mentioned microorganism can separate from natural world, buys from bacterial strain preservation person, or carries out artificial preparation according to the microbial technique of routine, technique for gene engineering etc. by mentioned microorganism.
For example, under the situation of separating from natural world, at first will be from open air collection soil.After being suspended in collected soil in the aqua sterilisa, the suspension that is obtained is administered to the solid culture medium that is used for separate microorganism such as bacillus subtilis, cultivates under 25 ℃ temperature.Grow independently bacterial clump after several days, downcut this bacterium colony and transfer to the solid culture medium that is used for separate microorganism such as bacillus subtilis, cultivate down at 25 ℃ again.The bacterium that grows is accredited as the bacterium that belongs to bacillus thuringiensis, according to SNEATH, (P.H.A.), MAIR, (N.S.), SHARPE, (M.E.) and HOLT, (J.G.); Method described in Bergey ' the s manual of SystematicBacteriology.Vol.2.1984.Williams and Wilkins, Baltimore, thus can select to belong to the bacterium of bacillus thuringiensis.
Next step, about the selected bacterium that belongs to bacillus thuringiensis, the existence of insecticidal proteins is confirmed according to evaluated biological activity method or microscopy observation method in the bacterium, thereby can select to belong to the bacterium of bacillus thuringiensis.
Employed albumen of the present invention can be albumen itself or for example culture, living cells, spore, its heat or the chemistry of bacillus thuringiensis are killed the insecticidal proteins that contains in form or its decomposed form among the present invention.
The example that is used to cultivate the medium of the bacterium that belongs to bacillus thuringiensis that can produce insecticidal proteins comprises general bacteria culture media and conventional meat soup liquid nutrient medium, can be any medium, as long as bacterium can grow.As indicated above, the culture of cultivating, contain bacillus thuringiensis and product thereof with above-mentioned medium can be in the centrifugal and/or dry back of the culture that will comprise medium as the another kind of active component of albumen of the present invention as the present composition.The insecticidal proteins itself that is separated according to known method by above-mentioned medium can directly be used as albumen of the present invention.By the way, the molecular weight of albumen of the present invention is generally tens thousand of to 120,000 dalton.When belonging to the bacterial decomposition of bacillus thuringiensis, albumen of the present invention discharges with spore with the crystalline material form after the cell division of certain number of times.
About the albumen of the present invention that uses in the present composition, can use commercially available BT preparation, preferably include Dipol (registration mark, by Sumitomo Chemical Co., the Ltd preparation), Esmark (registration mark, by Sumitomo Chemical Co., the Ltd preparation), Froback (registration mark, by Sumitomo Chemical Co., the Ltd preparation), (registration mark is by Sumika Takeda Co. for Zentari, the Ltd preparation), Novodor (registration mark, by Valent BioSciences Co. preparation), Buihunter (registration mark, by SumitomoChemical Co., the Ltd preparation; Business is transferred the possession of in December, 2003 by Kubota Biotech) and Design (registration mark is by Thermo Trilogy Co. preparation).
The present composition can be used for the wide-scale distribution of control agricultural and gardening pest insect (as insect and evil mite).Its general example comprises following insect.
Semiptera:
Delphacidae (Delphacidae) is as small brown rice planthopper (Laodelphax striatellus), brown planthopper (Nilaparvata lugens) and white-backed planthopper (Sogatella furcifera), angle top Cicadidae (Deltocephalidae) is as rice leafhopper (Nephotettix cincticeps) and Empoascaonukii, Aphidiadae (Aphididae) is as cotten aphid (Aphis gossypii), black peach aphid (Myzuspersicae) and turnip aphid (Lipaphis pserudobrassicae), Pentatomiddae (Pentatomidae), Aleyrodidae (Aleyrodidae) is as greenhouse whitefly (Trialeurodesvaporariorum), sweet potato whitefly (Bemisia tabaci) and little aleyrodid (Bemisiaargentifolli), a red-spotted lizard section (Coccidae), Tingidae (Tingidae), Psyllidae (Psyllidae) etc.
Lepidoptera:
Pyralidae (Pyralidae) is as striped rice borer (Chilo suppressalis), rice leaf roller (Cnaphalocrocis medinalis), European corn borer (Ostrinia nubilalis) and Parapediasia teterrella, Noctuidae (Noctuidae) is as the greedy noctuid (Spodopteralitura) of twill, greedy noctuid (Spodoptera exigua), oriental armyworm (Pseudaletia separata), tomato moth (Mamestra brassicae), black cutworm (Agrotis ipsilon), cabbage looper (Trichoplusia ni), stain gamma (Autographa nigrisigna), Heliothis (Heliothis spp.), Helicoverpa spp. and spark (Earias spp.), Sulfur butterfly (Pieridae) is as imported cabbageworm Japan subspecies (Pieris rapae crucivora), Adoxophyes honmai, adoxophyes moth (Adoxophyes orana fasciata), oriental fruit moth (Grapholita molesta) and apple skin steinernema (Cydia pomonella), moth fruit moth section (Carposinidae) is as peach fruit moth (Carposina niponensis), Bucculatricidae such as peach leaf miner (Lyonetia clerkella), Gracilariidae (Gracillariidae) is as the little thin moth (Phyllonorycter ringoniella) of diving of golden line, leaf lyonetid section (Phyllocnistidae) is as oranges and tangerines leaf lyonetid (Phylloenistis citrella), Yponomeutidae (Yponomeutidae) is as diamond-back moth (Plutella xylostella), Lymantriidae (Lymantriidae) is as Euproctis taiwana), gypsymoth (Lymantria dispar) and digramma brauni porthesia similis (fues.) (Euproctis similis), inferior moth section (Eucleidae) is as Scopelodescontracus, withered Noctuidae (Lasiocampidae) is as dendrolimus spectabilis (Dendrolimusspectabilis), Gelechidae (Gelechiidae) is as Pectinophora gossypiella (Pectinophoragossypiella), Arctiidae (Arctiidae), rain moth section (Tineidae) etc.
Diptera:
Agromyzidae (Agromyzidae) is as clover liriomyza bryoniae (Liriomyza trifolii), melon liriomyza bryoniae (Liriomyza bryoniae), vegetables liriomyza bryoniae (Liriomyza sativae), the color fly (Chromatomyia horticola) of diving of Liriomyza asterivora and pea, culex (Culex), yellow-fever mosquito (Aedes), anopheles (Anopheles), Chironomidae (Chironomidae), Nuscidae (Muscidae), Calliphoridae (Calliphoridae), Flesh flies (Sarcophagidae), Anthomyiidae (Anthomyiidae), Cecidomyiidae (Cecidomyiidae), Tephritidae (Trypetidae), Drosophilidae (Drosophilidae), Moth files (Psychodidae), Simulidae (Tabanidae), Tabanidae (Simuliidae), chela Nuscidae (Stomoxyinae) etc.
Coleoptera:
Chrysomelidae (Chrysomelidae), Scarabaeidae (Scarabaeidae), curculionid subfamily (Curculionidae), volume Elephantidae (Attelabidae), Coccinellidae (Coccinellidae), Cerambycidae (Cerambycidae), TRenebrionidae (Tenebrionidae) etc.;
Thrips:
Thripidae (Thripidae) comprises that as Thrips (Thrips spp.) joint melon thrips (Thripspalmi), flower thrips belong to (Frankliniella spp.) and comprise that west flower thrips (Frankliniellaoccidentalis), Sciltothrips spp. comprise Sciltothrips dorsalis, Phlaeothripidae (Phlaeothripidae) etc.
Thrips:
Joint melon thrips (Thrips palmi), yellow chest thrips (Thrips hawaiiensis) etc.
Hymenoptera:
Swaflies (Tenthredinidae), Dulicidae (Formicidae), Vespidae (Vespidae) etc.
Dictyoptera:
Blattidae (Blattidae), Ji Lian section (Blattellidae) etc.
Orthoptera:
Locust section (Acrididae), Gryllotalpidae (Gryllotalpidae) etc.
Siphonaptera:
Pulex irritans (Pulex irritans) etc.
Anoplura:
Head louse (Pediculus humanus capitis) etc.
Isoptera:
Termitidae (Termitidae) is as northern reticulitermes flavipe that dwells (Reticulitermes speratus) and coptotermes formosanus (Coptotermes formosanus) etc.
Acarina:
Mite section (Tetranychidae) belongs to (Panonychus spp.) as Tetranychus (Tetranychus spp.) and Panonychus citri, Eriophyidae (Eriophyidae) is as tomato peronium goitre mite (Aculopslycopersic), Tyroglyphidae (Acaridae), hard tick section (Ixodidae) etc.
The purposes of the present composition is not particularly limited, said composition can be used for preventing and treating the control of different types of plant and fruit tree pest insect on various agricultural and the gardening, as wild cabbage, Chinese cabbage, ChingGuang Juai, ternip, cauliflower, lettuce, spinach, tomato, eggplant, sweet green pepper, capsicum, cucumber, watermelon, muskmelon, asparagus, shallot, strawberry, rubber, rice, rye, wheat, barley, oat, grain, sweet potato, potato, corn, Kidney bean, broad bean, soybean, red bean, beet, sugarcane, tea, tobacco, forage, beet edible root, cocoa bean, carrot, cassava, celery, chickpea, cedra fruits, coconut, coffee, cotton, fig, grape, apple, sweet cherry, pears, peach, plum, persimmon, guava, gumbo, ornamental plants, papaya papaw, peanut, pepper, pigeonpea and pineapple.
The present invention also can protect flower such as turf, carnation, cherry, rose, flameray gerbera, chrysanthemum, pot plant and the Indian fig of cultivation.The example of cultivated plant also comprises all ornamental plantss and the shrub that blooms.
In the present composition, the mixing ratio of The compounds of this invention and albumen of the present invention can be the mixing ratio that produces synergistic effect, the example comprises 1: 10000-10000: 1, preferred 1: 1000-10: 1, more preferably 1: 1000-5: 1, also more preferably 1: 40-1: 1, preferred especially 1: 20-1: 1 weight ratio.
Under the situation of using the present composition, The compounds of this invention and albumen of the present invention can mix use, need not add any other composition, by adding solid carrier, liquid-carrier and carrier gas generally as required again, surfactant and other preparation used additives, said composition is preferably used with several formulations, as lubricant, emulsion, wetting powder, wettable granula, easily flow agent such as water dispersant and water emulsifier, pulvis, granule, aerosol, microcapsule formulations, hot fumigant and poison bait agent.The active component that the present composition can contain generally is approximately the weight ratio of 0.01-95% in above-mentioned preparation.
The example of the solid carrier that uses in preparation comprises fine powder and granular clay material (kaolin, diatomite, bentonite, Fubasami clay, Emathlite etc.), synthetic hydrated silica, various talcum, potter's clay, other inorganic minerals (sericite mica, quartz, sulphur, active carbon, calcium carbonate, hydrated SiO 2 etc.), chemical fertilizer (ammonium sulfate, ammonium phosphate, ammonium nitrate, urea, ammonium chloride etc.) etc.
The example of liquid-carrier comprises water, alcohol (methyl alcohol, ethanol etc.), ketone (acetone, MEK etc.), aromatic hydrocarbon (toluene, dimethylbenzene, ethylo benzene, methyl naphthalene etc.), non-aromatic hydrocarbon (hexane, cyclohexane, kerosene, light oil etc.), ester (ethyl acetate, butyl acetate etc.), nitrile (acetonitrile, isobutyronitrile etc.), ether (diisopropyl ether, diox etc.), acid amides (N, dinethylformamide, N,N-dimethylacetamide etc.), halogenated hydrocarbons (carrene, trichloroethanes, carbon tetrachloride etc.), methyl-sulfoxide, vegetable oil (soya-bean oil, cottonseed wet goods) etc.
Carrier gas, promptly the example of propellant comprises fluorine Lyons gas, butagas, liquefied petroleum gas, dimethyl ether and carbonic acid gas.
The example of surfactant comprises alkyl sulfate, alkylsulfonate, alkylaryl sulfonate, alkyl aryl ether and polyoxyethylene derivative thereof, polyglycol ether, polyalcohols ester, sugar alcohol derivant etc.
The example of other preparation used additives comprises casein, gelatin, carbohydrate (starch, gum Arabic, cellulose derivatives, alginic acid etc.), lignin derivative, bentonite, synthetic water soluble (CO) polymers (polyvinyl alcohol, polyvinylpyrrolidone, polyacrylic acid etc.), PAP (acid isopropyl base phosphate), BHT (2,6 di tert butyl 4 methyl phenol), BHA (the 2-tert-butyl group-4-metoxyphenol) and the 3-tert-butyl group-4-metoxyphenol), vegetable oil, mineral oil, surfactant, fatty acid, fatty acid ester etc.
The example of the base-material that uses in the poison bait agent comprises bait such as grain dust, vegetable oil, sugar and avicel cellulose; Antioxidant such as dibutyl hydroxy toluene and nordihydroguaiaretic acid; Eat inhibitor such as chilli powder by mistake and lure spices such as cheese spices and onion spice.
Easily the stream agent can constantly be dispersed in by active compound component that will about 1-75% weight as water dispersant and water emulsifier in the water of auxiliary agent (as anti-foaming agent, antirust agent, stabilizing agent, spreader-sticker, penetrant, anticoagulant, preservative and bactericide) of the suspension aids that contains the 0.5-15% weight of the having an appointment material of protective colloid and thixotropy characteristic (as can produce) and about 0-10% weight and obtain.
The present composition also can be processed each active component by above-mentioned preparation technique, mixes above-mentioned preparation afterwards again and prepares.That is to say that the present composition can prepare by the composition that mixing comprises the composition of above-mentioned processed The compounds of this invention and comprises above-mentioned processed albumen of the present invention according to its preparation.Both also can mix use or use together in the process of using for they.
So the present composition of processing can directly use or dilute with water after use etc.In addition, other insecticide, miticide, nematocide, fungicide, weed killer herbicide, plant growth regulator, synergist, fertilizer, soil conditioner, animal feed etc. also can mix use or use together.
The present composition can be administered on the habitat and claimed plant of insect, insect.In the case, when being processed into emulsion, wetting powder, wettable granula and easily flowing agent, the general dilute with water of the present composition is handled.
Handle at the present composition under the situation of agricultural and gardening pest insect, the effective dose of the present composition or amount of application are generally about 0.1-1000g/1000m
2When the active component of pest control was used as granule, pulvis, lubricant etc., the present composition can generally direct use, need not dilute, to obtain above-mentioned effective dose or amount of application.
Even at the active component of pest control by dilute with water emulsion, wetting powder, wettable granula, easily flow under the situation that agent etc. uses, present composition dilutable water is used, to obtain above-mentioned effective dose or amount of application.Emulsion therein, wetting powder, wettable granula, easily flow under the situation that agent etc. uses by dilute with water, the instantiation of the application concentration of the present composition comprises general about 1-10000ppm.
Each can change above-mentioned effective dose and amount of application with the degree of kind, the time of application of situation such as preparation, the kind of using place, application process, insect and infringement, and can be by increasing outside above-mentioned scope or reducing and suitably select.
Embodiment
Hereinafter be described in further detail the present invention, but be not restricted to this by example of formulations and experimental example.
The culture (being a kind of form of albumen of the present invention) that above-mentioned " BT bacterial cultures " expression obtains in the following way contains 1 * 10 in the 1ml spore suspension
3The spore alive by the bacterial derivation that belongs to bacillus thuringiensis, getting 100 μ L is poured in the flask of 500ml, be inoculated in the L-meat soup liquid nutrient medium (100ml) of sterilization, concussion was cultivated (150rpm) about 2-5 days under 25 ℃ temperature condition, afterwards from reclaiming dry spore also and by the insecticidal proteins of the bacterial derivation that belongs to bacillus thuringiensis from the culture that obtains by centrifugal.
Example of formulations 1 emulsion
1 part of The compounds of this invention and 10 parts of BT bacterial culturess are dissolved in 36.5 parts of dimethylbenzene and the 36.5 parts of dimethyl formamides,, fully mix acquisition emulsion to wherein adding 10 parts of polyoxyethylene styrene phenyl ethers and 6 parts of calcium dodecyl benzene sulfonates.
Example of formulations 2 wetting powders
5 parts of The compounds of this invention and 45 parts of BT bacterial culturess are joined in 4 parts of NaLSs, 2 parts of calcium lignosulfonates, 22 parts of synthetic moisture fine silica and the 22 parts of diatomaceous mixtures, fully mix the acquisition wetting powder.
Example of formulations 3 granules
Join 5 parts of BT bacterial culturess, 5 parts of synthetic aqueous silicon dioxide fine powders, 5 parts of neopelexes, 30 parts of bentonites and 54 parts of clays in 1 part of The compounds of this invention and fully mix; in this mixture, add an amount of water subsequently; stir again; by the comminutor granulation, pneumatic conveying drying obtains granule.
Example of formulations 4 dusting powders
With 4 parts of BT bacterial culturess, 0.5 part of The compounds of this invention, 1 part of synthetic moisture fine silica, 1 part of Dorires B as flocculant (by Sankyo Co., Ltd. the preparation) and 7 parts of clays fully mix with mortar, mix with the fruit juice agitator then.In the mixture of gained, add 86.5 parts cut clay and fully mix the acquisition dusting powder.
Easily stream agent of example of formulations 5
Join 1 part of The compounds of this invention, 10 parts of BT bacterial culturess and 1.5 parts of sorbitan trioleates in 26 parts of aqueous solution that contain 2 parts of polyvinyl alcohol and fully mix, this mixture is subsequently by sand mill pulverize (particle diameter 3 μ m or littler), add 48 parts of aqueous solution that contain 0.05 part of xanthans and 0.1 part of Magnesiumaluminumsilicate afterwards, add 15 parts of propane diols again, mix and obtain easily stream agent.
Example of formulations 6 lubricants
In 5 parts of dimethylbenzene and 5 parts of trichloroethanes, dissolve 0.1 part of The compounds of this invention and 0.8 part of BT bacterial cultures, with the kerosene mixing acquisition lubricant of 89.1 parts of deodorizing.
Example of formulations 7 microcapsule formulations
Mix 2 parts of The compounds of this invention, 8 parts of BT bacterial culturess, 10 parts of phenyl xylyl ethane and 0.5 part of Sumijoule L-75 (two isocyanic acid benzal ester (tolylenediisocyanate), by Sumitomo Bayer Urethane Ltd. preparation), join afterwards in 20 part 10% the Arabic gum aqueous solution, stir by even mixer that to obtain average grain diameter be the emulsion of 20 μ m.Next step, to wherein adding 2 parts of ethylene glycol, reaction obtained microcapsule slurry in 24 hours in 60 ℃ temperature is bathed again.On the other hand, 0.2 part of xanthans and 1.0 parts of Beagum R (Magnesiumaluminumsilicate, by Sanyo Kasei Co., Ltd. preparation) are dispersed in 56.3 parts of ion exchange waters obtain thickener soln.Mix 42.5 parts of above-mentioned microcapsule slurry and above-mentioned 57.5 parts of acquisitions of thickener soln microcapsules.
In addition, experimental example shows that the present composition shows good pest controling effect.Under the situation of mixing two kinds of active components, the theoretical value of desired killing rate is by E=X+Y-(X * Y/100) (by the way, this formula generally is called the Colby computing formula, is used to calculate the standard of the synergistic effect and the antagonistic effect of agent combination) calculate.In this formula,
X: the killing rate (%) when handling with the active components A of M ppm
Y: the killing rate (%) when the active component B of usefulness N ppm handles
E: the theoretical value of the killing rate of expection when the active component B of the active components A of usefulness M ppm and N ppm handles respectively
Experimental example 1 (to the artificial feed test of tobacco budworm (Heliothis virescens))
10% emulsion and BT bacteria preparation Design (registration mark) (the bacillus thuringiensis catfish pool mutation GC91 bacterial strain (conjugant) that mix The compounds of this invention, the wettable granula, by Thermo Trilogy Corporation preparation) diluent water prepare testing liquid, wherein each is diluted to predetermined concentration.After the bottom surface of plastic cup (capacity of 30ml) adds artificial feed (about 2g), handle with the testing liquid of 0.2ml.Handle the back after air-dry about 2 hours, discharge the second instar larvae (20 repetitions) of a test insect (tobacco budworm).Handle and measured lethality in back 5 days, and proofread and correct, thereby calculate killing rate (%) according to following formula.The results are shown in the table 1.
Killing rate (%)=100 * (Mt-Mc)/(100-Mc)
Mt: the lethality of test compound treatment region (%)
Mc: the lethality (%) that test compound is untreated and distinguishes
Table 1
| Test reagent | Application concentration (ppm) | The theoretical value of killing rate (%) | The actual measured value of killing rate (%) | The judged result that synergistic effect exists |
| The The compounds of this invention albumen present composition of the present invention | 1.56 15.6 1.56+15.6 | 11.1 | 0.0 11.1 88.3 | --exist |
| The The compounds of this invention albumen present composition of the present invention | 6.25 62.5 6.25+62.5 | 63.9 | 27.8 50.0 100.0 | --exist |
Experimental example 2 (to the edible leaf immersion test of the greedy noctuid (Spodoptera litura) of twill)
Mix 10% emulsion and BT bacteria preparation Froback (registration mark) (bacillus thuringiensis catfish pool mutation of The compounds of this invention, do easily stream agent, by Sumitomo Chemical Co., Ltd preparation) diluent water obtain wherein each be diluted to the thinner of predetermined concentration, in this thinner, add spreader-sticker with respect to thinner 1/5000 amount, thus the preparation testing liquid.Potted plant cabbage leaves is cut during the phase and is immersed in this testing liquid at the 5-8 leaf.With diameter is that the filter paper of 90mm places plastic cup (capacity of 500ml), places the air-dry cabbage leaves in dipping back on filter paper.On wild cabbage, place 3 instar larvaes (3 repetitions) of 10 test insects (twill is coveted noctuid).Handle and measured the test insect mortality in back 6 days, and proofread and correct, thereby calculate killing rate (%) according to following formula.The results are shown in the table 2.
Killing rate (%)=100 * (Mt-Mc)/(100-Mc)
Mt: the lethality of test compound treatment region (%)
Mc: the lethality (%) that test compound is untreated and distinguishes
Table 2
| Test reagent | Application concentration (ppm) | The theoretical value of killing rate (%) | The actual measured value of killing rate (%) | The judged result that synergistic effect exists |
| The The compounds of this invention albumen present composition of the present invention | 0.78 25.0 0.78+25.0 | 44.0 | 30.0 20.0 56.7 | --exist |
| The The compounds of this invention albumen present composition of the present invention | 1.56 25.0 1.56+25.0 | 44.0 | 30.0 20.0 76.7 | --exist |
| The The compounds of this invention albumen present composition of the present invention | 1.56 12.5 1.56+12.5 | 37.0 | 30.0 10.0 90.0 | --exist |
| The The compounds of this invention albumen present composition of the present invention | 1.56 6.25 1.56+6.25 | 37.0 | 30.0 10.0 86.0 | --exist |
Industrial applicibility
The present invention can provide pest control composition, and it has the synergistic effect than adding of obtaining of simple mixing and better effects if, and has reduced using dosage.
Claims (8)
1. pest control composition, comprise 3,5-two chloro-1-(3,3-two chloro-2-acrylic oxygen bases)-and 4-[3-(5-5-flumethiazine-2-base oxygen base) propyl group oxygen base] insecticidal proteins of benzene and bacillus thuringiensis (Bacillus thuringiesis), wherein 3,5-two chloro-1-(3,3-two chloro-2-acrylic oxygen bases)-4-[3-(5-5-flumethiazine-2-base oxygen base) propyl group oxygen base] weight ratio of insecticidal proteins of benzene and bacillus thuringiensis is 1: 1000-10: 1.
2. according to the pest control composition of claim 1, wherein said insecticidal proteins is that culture, living cells, spore, its heat or the chemistry of bacillus thuringiensis killed the insecticidal proteins that contains in form or its decomposed form.
3. insect pest control method; comprise to the habitat of claimed plant, insect or insect and use 3 of synergy effective dose; 5-two chloro-1-(3; 3-two chloro-2-acrylic oxygen bases)-and 4-[3-(5-5-flumethiazine-2-base oxygen base) propyl group oxygen base] insecticidal proteins of benzene and bacillus thuringiensis; wherein 3; 5-two chloro-1-(3,3-two chloro-2-acrylic oxygen bases)-4-[3-(5-5-flumethiazine-2-base oxygen base) propyl group oxygen base] weight ratio of insecticidal proteins of benzene and bacillus thuringiensis is 1: 1000-10: 1.
4. according to the method for claim 3, wherein amount of application is about 0.1-1000g/1000m
23,5-two chloro-1-(3,3-two chloro-2-acrylic oxygen bases)-4-[3-(5-5-flumethiazine-2-base oxygen base) propyl group oxygen base] combination of insecticidal proteins of benzene and bacillus thuringiensis.
5.3,5-two chloro-1-(3,3-two chloro-2-acrylic oxygen bases)-and 4-[3-(5-5-flumethiazine-2-base oxygen base) propyl group oxygen base] insecticidal proteins of benzene and bacillus thuringiensis is used for the combined use of control of insect, wherein 3,5-two chloro-1-(3,3-two chloro-2-acrylic oxygen bases)-4-[3-(5-5-flumethiazine-2-base oxygen base) propyl group oxygen base] weight ratio of insecticidal proteins of benzene and bacillus thuringiensis is 1: 1000-10: 1.
6. according to the pest control composition of claim 1 or 2, wherein 3,5-two chloro-1-(3,3-two chloro-2-acrylic oxygen bases)-4-[3-(5-5-flumethiazine-2-base oxygen base) propyl group oxygen base] weight ratio of insecticidal proteins of benzene and bacillus thuringiensis is 1: 40-1: 1.
7. according to the insect pest control method of claim 3 or 4, wherein 3,5-two chloro-1-(3,3-two chloro-2-acrylic oxygen bases)-4-[3-(5-5-flumethiazine-2-base oxygen base) propyl group oxygen base] weight ratio of insecticidal proteins of benzene and bacillus thuringiensis is 1: 40-1: 1.
8. according to the combined use of claim 5, wherein 3,5-two chloro-1-(3,3-two chloro-2-acrylic oxygen bases)-4-[3-(5-5-flumethiazine-2-base oxygen base) propyl group oxygen base] weight ratio of insecticidal proteins of benzene and bacillus thuringiensis is 1: 40-1: 1.
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| Application Number | Priority Date | Filing Date | Title |
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| JP2003354762 | 2003-10-15 | ||
| JP354762/2003 | 2003-10-15 |
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| WO (1) | WO2005034627A1 (en) |
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| TW200518676A (en) | 2005-06-16 |
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