WO2005001108A3 - Method and device for characterising a culture solution - Google Patents

Method and device for characterising a culture solution Download PDF

Info

Publication number
WO2005001108A3
WO2005001108A3 PCT/EP2004/007034 EP2004007034W WO2005001108A3 WO 2005001108 A3 WO2005001108 A3 WO 2005001108A3 EP 2004007034 W EP2004007034 W EP 2004007034W WO 2005001108 A3 WO2005001108 A3 WO 2005001108A3
Authority
WO
WIPO (PCT)
Prior art keywords
culture solution
cells
product
parameters
characterising
Prior art date
Application number
PCT/EP2004/007034
Other languages
German (de)
French (fr)
Other versions
WO2005001108A2 (en
Inventor
Frank Theodor Gudermann
Marc Stuerz
Original Assignee
Innovatis Ag
Frank Theodor Gudermann
Marc Stuerz
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Innovatis Ag, Frank Theodor Gudermann, Marc Stuerz filed Critical Innovatis Ag
Publication of WO2005001108A2 publication Critical patent/WO2005001108A2/en
Publication of WO2005001108A3 publication Critical patent/WO2005001108A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/32—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Sustainable Development (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The invention relates to a method and a device for determining specific product formation rates and/or substrate consumption rates of cells in a culture solution. Determination of the specific formation and/or consumption rates is based on measurement of the parameters of the number of living cells, cell viability and the product and substrate concentration in a cell culture sample whereby the culture solution is analysed by microscopic imaging of the cells and by evaluating said imaging and by determining the product and substrate concentration, more particularly, by means of SPR (surface plasmon resonance). Analysis of the parameters of said sample is carried out simultaneously, in an automatic manner.
PCT/EP2004/007034 2003-06-30 2004-06-29 Method and device for characterising a culture solution WO2005001108A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10329539.9 2003-06-30
DE2003129539 DE10329539A1 (en) 2003-06-30 2003-06-30 Method and device for characterizing a culture fluid

Publications (2)

Publication Number Publication Date
WO2005001108A2 WO2005001108A2 (en) 2005-01-06
WO2005001108A3 true WO2005001108A3 (en) 2009-03-12

Family

ID=33521223

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2004/007034 WO2005001108A2 (en) 2003-06-30 2004-06-29 Method and device for characterising a culture solution

Country Status (2)

Country Link
DE (1) DE10329539A1 (en)
WO (1) WO2005001108A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102005021034B4 (en) * 2005-05-06 2012-09-13 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Method for culturing a cell culture in an automated cell culture system and automated cell culture system
DE102006022877B4 (en) 2006-05-15 2009-01-29 Sartorius Stedim Biotech Gmbh Method for determining the viability of cells in cell cultures
DE102007042138A1 (en) * 2007-09-05 2009-03-12 Carl Zeiss Microimaging Gmbh Method and device for automatic sample feeding

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0333560A1 (en) * 1988-03-08 1989-09-20 Chemunex Process for quantitative determination of micro organismes
JPH03123481A (en) * 1989-10-06 1991-05-27 Toa Denpa Kogyo Kk Apparatus for automatic simultaneous determination of cell concentration and cell activity
JPH04148673A (en) * 1990-10-09 1992-05-21 Kao Corp Diagnosis of culture state
WO1999050442A1 (en) * 1998-03-30 1999-10-07 Friedrich Srienc A flow injection flow cytometry system for on-line monitoring of bioreactors and method for monitoring
WO1999061577A1 (en) * 1998-05-22 1999-12-02 Microalgae S.P.A. Closed circuit photobioreactor

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0333560A1 (en) * 1988-03-08 1989-09-20 Chemunex Process for quantitative determination of micro organismes
JPH03123481A (en) * 1989-10-06 1991-05-27 Toa Denpa Kogyo Kk Apparatus for automatic simultaneous determination of cell concentration and cell activity
JPH04148673A (en) * 1990-10-09 1992-05-21 Kao Corp Diagnosis of culture state
WO1999050442A1 (en) * 1998-03-30 1999-10-07 Friedrich Srienc A flow injection flow cytometry system for on-line monitoring of bioreactors and method for monitoring
WO1999061577A1 (en) * 1998-05-22 1999-12-02 Microalgae S.P.A. Closed circuit photobioreactor

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
PATENT ABSTRACTS OF JAPAN vol. 015, no. 324 (C - 0859) 19 August 1991 (1991-08-19) *
PATENT ABSTRACTS OF JAPAN vol. 016, no. 431 (C - 0983) 9 September 1992 (1992-09-09) *

Also Published As

Publication number Publication date
WO2005001108A2 (en) 2005-01-06
DE10329539A1 (en) 2005-01-20

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