Classifications

• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/63—Compounds containing para-N-benzenesulfonyl-N-groups, e.g. sulfanilamide, p-nitrobenzenesulfonyl hydrazide
  • A61K31/635—Compounds containing para-N-benzenesulfonyl-N-groups, e.g. sulfanilamide, p-nitrobenzenesulfonyl hydrazide having a heterocyclic ring, e.g. sulfadiazine
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/33—Heterocyclic compounds
  • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
  • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
  • A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P35/00—Antineoplastic agents
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00

Definitions

• This invention pertains to a composition
• a composition comprising one or more cancer drugs, continuous oral dosing schedule with drugs which bind to the colchicine site of tubulin ⁇ -subunits, and methods of treating diseases using continuous dosing schedules.
• One embodiment of this invention pertains to a continuous oral dosing schedule for treatment of disease in a human with a therapeutically acceptable amount of a drug, or a therapeutically acceptable salt thereof, which binds to tubulin ⁇ -subunits, wherein said dosing schedule lasts for at least five days.
• Another embodiment pertains to a continuous oral dosing schedule for treatment of disease in a human with a therapeutically acceptable amount of a drug, or a therapeutically acceptable salt thereof, which binds to tubulin ⁇ -subunits, wherein said dosing schedule lasts for at least five days and during which the severity of at least one adverse side effect selected from the group consisting of anemia, alopecia, fluid retention, myelosupression, neuropathy and neutropenia is essentially reduced when compared to the severity of the same side effect coincident with treatment of the substantially same disease with a parenterally administered drug which binds to tubulin ⁇ -subunits.
• Still another embodiment pertains to a continuous oral dosing schedule for treatment of disease in a human with a therapeutically acceptable amount of a drug which binds to the colchicine site of tubulin ⁇ -subunits, or a therapeutically acceptable salt thereof, wherein said dosing schedule lasts for at least five days.
• Still another embodiment pertains to a continuous oral dosing schedule for treating disease in a human with a therapeutically acceptable amount of a drug, or a therapeutically acceptable salt thereof, which binds to the colchicine site of tubulin ⁇ -subunits, wherein said dosing schedule lasts for at least five days and during which the severity of at least one adverse side effect selected from the group consisting of anemia, alopecia, fluid retention, myelosupression, neuropathy and neutropenia is essentially reduced when compared to the severity of the same side effect coincident with treatment of the substantially same disease with a parenterally administered drug which binds to tubulin ⁇ -subunits .
• Still another embodiment of this invention pertains to a continuous oral dosing schedule for treatment of disease in a human with a therapeutically acceptable amount of N- (2- ( (4-hydroxyphenyl ) amino)pyrid-3-yl) -4-methoxy- benzenesulfonamide, or a therapeutically acceptable salt thereof, wherein said dosing schedule lasts for at least five days .
• Still another embodiment pertains to a continuous oral dosing schedule for treatment of disease in a human with a therapeutically acceptable amount of N- (2- ( (4-hydroxyphenyl) - amino)pyrid-3-yl) -4-methoxybenzenesulfonamide, or a therapeutically acceptable salt thereof, wherein said dosing schedule lasts for at least five days, and during which the severity of at least one adverse side effect selected from the group consisting of anemia, alopecia, fluid retention, myelosupression, neuropathy and neutropenia is essentially reduced when compared to the severity of the same side effect coincident with treatment of the substantially same disease with a parenterally administered drug which binds to tubulin ⁇ -subunits .
• Still another embodiment pertains to a method for treatment of disease in a human, said method comprising continuously orally administering, for at least five days, a therapeutically acceptable amount of a drug, or a therapeutically acceptable salt thereof, which binds to tubulin ⁇ -subunits.
• Still another embodiment pertains to a method for treatment of disease in a human, said method comprising continuously orally administering, for at least five days, a therapeutically acceptable amount of a drug, or a therapeutically acceptable salt thereof, which binds to tubulin ⁇ -subunits, during which dosing schedule the severity of at least one adverse side effect selected from the group consisting of anemia, alopecia, fluid retention, myelosupression, neuropathy and neutropenia is essentially reduced when compared with treatment of the substantially same disease with a parenterally administered drug which binds to tubulin ⁇ -subunits.
• Still another embodiment pertains to a method for treatment of disease in a human, said method comprising continuously orally administering, for a time period of at least five days, a therapeutically acceptable amount of a drug, or a therapeutically acceptable salt thereof, which binds to the colchicine site of tubulin ⁇ -subunits. Still another embodiment pertains to a method for treatment of disease in a human, said method comprising continuously orally administering, for at least five days, a
• a drug or a therapeutically acceptable salt thereof, which binds to the colchicine site of tubulin ⁇ -subunits, during which dosing schedule, the severity of at least one adverse side effect selected from the group consisting of anemia, alopecia, fluid
• Still another embodiment pertains to a method for treatment of disease in a human, said method comprising continuously orally administering, for at least five days, a therapeutically acceptable amount of N- (2- ( (4-hydroxyphenyl) - amino)pyrid-3 -yl) -4-methoxybenzenesulfonamide, or a
• Still another embodiment pertains to a method for treatment of disease in a human, said method comprising continuously orally administering, for at least five days, a therapeutically acceptable amount of N- (2- ( (4-hydroxyphenyl) - 125 amino)pyrid-3-yl) -4-methoxybenzenesulfonamide, or a therapeutically acceptable salt thereof, during which dosing schedule, the severity of at least one adverse side effect selected from the group consisting of anemia, alopecia, fluid retention, myelosupression, neuropathy and neutropenia is
• Still another embodiment pertains to a composition for 135 immediate gastrointestinal release of N-(2-((4- hydroxyphenyl) amino)pyrid-3-yl) -4-methoxybenzenesulfonamide comprising a therapeutically effective amount of N-(2-((4- hydroxyphenyl ) amino) pyrid-3 -yl ) -4-methoxybenzenesulfonamide and an excipient, which composition induces, upon continuous
• Still another embodiment pertains to a pharmaceutical composition having therapeutic synergy comprising N-(2-((4- hydroxyphenyl ) amino) pyrid-3 -yl) -4-methoxybenzenesulfonamide
• cancer drug 150 and at lease one cancer drug selected from the group consisting of cisplatin, docetaxel, and 5-fluorouracil .
• Still another embodiment pertains to a method of treating cancer in a human comprising administering a therapeutically effective amount of N- (2- ( (4-hydroxyphenyl) -amino) pyrid-3-yl) -
• At least five days means the time period over which the drug is administered.
• at least five days means for the first 7 days of a 21 day schedule, for the first 14 days of a 21 day schedule, for he 165 first 15 days of a 21 day schedule, for the first 21 days of a 28 day schedule, for 5 days then cessation for 5 days then continuation for 5 days then cessation for 5 days, i.e. (5 days on/5 days off)x2, and for 7 days then cessation for 7 days then continuation for 7 days then cessation for 7 days,
• tubulin site binder means a tubulin ⁇ -subunit binder which binds to the colchicine site of the tubulin ⁇ -subunits and thereby inhibits the polymerization of tubulin.
• a preferred example of a drug which binds to the colchicine site of tubulin ⁇ -subunits for the practice of this invention is N- (2- ( (4-hydroxyphenyl) amino) pyrid-3 -yl) -4- methoxybenzenesulfonamide, also referred to herein as N- (2- ( (4-hydroxyphenyl) amino) pyrid-3 -yl) -4-
• cancer means bone marrow dyscrasias, breast (ductal and lobular) cancer, cervical cancer, colon cancer, leukemia, lung (small cell and non-small cell) cancer, lymphoma, melonoma, mouth and tongue cancer, pancreatic cancer, prostate cancer, rectal cancer, renal
• examples of diseases for which drugs which bind to the colchicine site of tubulin ⁇ -subunits are useful are gouty arthritis and cancer.
• drug means a compound which
• parenterally administered drugs include vinca alkaloids (vincristine, vinblastine, and vinorelbine) , taxanes (paclitaxel and docetaxel), 5-fluorouracil, cisplatin,
• docetaxel such as colchicine itself which is used to treat gouty arthritis.
• colchicine site binders such as colchicine itself which is used to treat gouty arthritis.
• Binding affinities of N- (2- ( (4-hydroxyphenyl) amino)pyrid- 3-yl) -4-methoxybenzenesulfonamide, vinblastine, and paclitaxel were evaluated usi ⁇ ng the competi.tion of [3H] colchicine to 225 biotinylated bovine brain tubulin in a scintillation proximity assay.
• 235 not colchicine-site binders, and therefore must bind to tubulin ⁇ -subunits sites which are different from the colchicine binding site.
• N- (2- ( (4-hydroxyphenyl) amino) pyrid-3 -yl) -4- methoxybenzenesulfonamide is a colchicine site binder
• 240 exemplifies drugs which are useful for treatment of diseases which may be treated with colchicine-site binders other than colchicine itself.
• This invention pertains to an unexpected and surprising combination of variables which lead to a favorable therapeutic event with a sufficient reduction in the
• M5076 is a transplantable murine reticulum cell sarcoma.
• N- [ 2 - ( (4-hydroxyphenyl) mino) pyrid-3-yl) -4- methoxybenzenesulfonamide exhibited significant antitumor activity in this syngeneic flank tumor model when administered
• N- (2- ( (4-hydroxyphenyl) amino) pyrid-3 -yl) -4- ethoxybenzenesulfonamide significantly inhibited tumor growth with T/C (tumor mass of test group divided by tumor mass of control group) and ILS (percent increase in life span) values
• Ap P ⁇ n (Min) mice are models for genetically inherited ⁇ intestinal cancer. These mice carry a dominant germline mutation in the Ape tumor suppressor gene that predisposes them to the development of numerous (>50) tumors throughout
• NCI-H460 is a human non-small cell lung carcinoma derived cell line. It is MDR negative, has wild type p53 , and
• HCT-15 is a human colon carcinoma derived cell line. It is MDR positive, and expresses both mutant p53 and oncogenic
• the HCT-15 cell line has one of the highest levels of m r-1/P-glycoprotein expression of cells from the NCI tumor cell line panel.
• Paclitaxel and vincristine which are both substrates for P-glycoprotein drug efflux pump, were not efficacious, while (2- ( (4-hydroxyphenyl) amino) pyrid-3-yl) -4-
• MDA-MB-468 ⁇ " ⁇ 00 " QD d " 10-14 , " “ 25 “ 78 (2- ( (4-hydroxyphenyl) amino) pyrid-3-yl) -4- methoxybenzenesulfonamide also showed antitumor activity against a variety of other human tumor xenografts in nude mice .
• Antitumor activity was also seen in tumors derived from gastric, lung, breast, and oral carcinomas.
• the dose limiting toxicities were Grade 3 peripheral neuropathy in 1 of 4 subjects at 210 mg/m 2 /day and Grade 4 intestinal paralysis in 1 of 4 subjects at 210 mg/m 2 /day and in 1 of 6 subjects at 240 mg/m 2 /day.
• the 250 mg QD dose has been determined to be the MTD, as dose limiting toxicities of peripheral neuropathy/ileus were reported in 2 of 6 subjects at the 300 mg QD dose.
• the MTD of the QD regimen given for 21 days was determined to be 200 mg as dose limiting toxicities
• tubulin ⁇ -subunit binders of this invention can be administered with or without an excipient.
• Excipients include encapsulating materials or additives such as absorption accelerators, antioxidants, binders, buffers, coating agents, coloring agents, diluents,
• 390 invention to be administered orally include agar, alginic acid, aluminum hydroxide, benzyl alcohol, benzyl benzoate, 1,3-butylene glycol, castor oil, cellulose, cellulose acetate, cocoa butter, corn starch, corn oil, cottonseed oil, ethanol, ethyl cellulose, ethyl laureate, ethyl oleate, gelatin, germ
• tubulin ⁇ -subunit binders of this invention osmotically include chlorofluorohydrocarbons , ethanol, water and mixtures thereof.
• Excipients for the tubulin ⁇ -subunit binders of this invention to be administered parenterally include 1, 3-butanediol, castor oil, corn oil, cottonseed oil, germ oil, groundnut oil, liposomes, oleic acid, olive oil,
• tubulin ⁇ -subunit binders of this invention to be administered rectally or vaginally include cocoa butter, polyethylene
• ABT-751 30.0 microcrystalline 15.8 Filler cellulose
• lactose, and croscarmellose were granulated with a solution of povidone in water, dried, and milled. The milled product was blended with magnesium stearate.
• the doses herein were made by filling capsules with the appropriate amount of blended product .
• tubulin ⁇ -subunit binders of this invention may be administered orally, ophthalmically, osmotically, parenterally (subcutaneously, intramuscularly, intrasternally, intravenously) , rectally, topically, transdermally, or
• Orally administered solid dosage forms can be administered as capsules, dragees , granules, pills, powders, or tablets .
• Ophthalmically and orally administered dosage forms may be administered as elixirs, emulsions, microemulsions , suspensions, or syrups. Osmotically and
• topically administered dosage forms may be administered as creams, gels, inhalants, lotions, ointments, pastes, or powders.
• Parenterally administered dosage forms may be administered as aqueous or oleaginous suspensions.
• Rectally and vaginally dosage forms may be administered as creams,
• tubulin ⁇ -subunit binders of this invention administration of drugs which bind to the colchicine binding site of tubulin ⁇ -subunits is more preferable than parenteral administration of the same drug.
• the composition containing the tubulin ⁇ -subunit binder depend on the recipient of treatment, the disease being treated and the severity thereof, the composition containing the tubulin ⁇ -subunit binder, the time of administration, the route of administration, the potency of the tubulin ⁇ -subunit binder, the rate of clearance of the tubulin ⁇ -subunit binder,
• the daily amount of N- (2- ( (4-hydroxyphenyl) amino) pyrid-3 - yl) -4-methoxybenzenesulfonamide to be administered orally in a continuous, once daily dose to adult patients having refractory solid tumors is about 50 mg, about 100 mg, about
• about 250 mg of N-(2-((4- hydroxyphenyl) amino) pyrid-3 -yl) -4-methoxybenzenesulfonamide is continuously administered orally once per day (QD) to adult patients having refractory solid tumors for the first 7 days
• N-(2-((4- hydroxyphenyl) amino) pyrid-3 -yl) -4-methoxybenzenesulfonamide is continuously administered orally once per day to adult patients having refractory solid tumors for the first 21 days
• N-(2-((4- hydroxyphenyl ) amino) pyrid-3 -yl) -4-methoxybenzenesulfonamide is administered continuously orally once per day to adult patients having breast lung, kidney, or colon cancer, is for
• the daily amount of N- (2- ( (4-hydroxyphenyl) amino) pyrid-3 - yl) -4-methoxybenzenesulfonamide to be administered orally in a continuous once per day dose to pediatric patients having refractory solid tumors may be about 100 mg/mm , about 130
• 495 refractory hematologic malignancies may be about 100 mg/mm 2 , about 125 mg/mm 2 , and about 150 mg/mm2.
• the daily amount of N- (2- ( (4-hydroxyphenyl) amino) pyrid-3- yl) -4-methoxybenzenesulfonamide to be administered orally in continuous, twice daily (BID) doses to adult patients having 500 refractory solid tumors may be about 25 mg, about 50 mg, about 75 mg, about 100 mg, about 125 mg, about 150 mg, about
• N-(2-((4- hydroxyphenyl) amino) pyrid-3 -yl) -4-methoxybenzenesulfonamide is 505 administered orally twice per day to adult patients having refractory solid tumors for the first 7 days of a 21 day schedule .
• the daily amount of N- (2- ( (4-hydroxyphenyl) amino) pyrid-3- yl) -4-methoxybenzenesulfonamide to be administered orally in 510 continuous twice per day doses to adult patients having refractory hematologi ⁇ c mali ⁇ gnancies may be about 75 mg/mm2 ,
• 515 continuous twice per day doses to pediatric patients having refractory solid tumors may be about 100 mg/mm and about 130 mg/mm .
• N- (2- ( (4-hydroxyphenyl) amino) pyrid-3-yl) -4- methoxybenzenesulfonamide may also be useful in the treatment
• the compounds of the invention when used alone or in combination with other therapies, may be administered alone or in combination with radiotherapy, hormonal agents, antibodies, antiangiogenics, COX-2 inhibitors, or other 525 chemotherapeutic agents (cytotoxic or cytostatic) such as cisplatin, 5-fluorouracil, taxotere, docetaxel and gemcitabine.
• chemotherapeutic agents cytotoxic or cytostatic
• 530 chemotherapeutic drugs was tested in Calu-6 NSCL, MDA-MB-468 breast and HT-29 colon carcinoma xenografts which were grown in nude mice.
• tumors were passaged three times in mice before using the fragments to generate single cell suspensions. Multiple animals were used as donors . Single cell suspensions were generated by homogenizing the minced tumor tissue in RPMI 1640 media.
• 555 suspensions were washed three times to remove debris and other foreign materials .
• Viable cells were counted by trypan blue exclusion technique using a hemocytometer . Mice were injected subcutaneously with 0.5 mL of the cell suspension containing
• IxlO 6 cells 560
• 50 mg/kg/day was selected because administration at this dose was essentially without adverse side effects.
• mice bearing established tumors were size matched and grouped at about 233 mm 3 .
• N-(2-((4- hydroxyphenyl) amino) pyrid-3 -yl) -4-methoxybenzenesulfonamide at 575 50 mg/kg/day administered either on a once per day or twice per day schedule demonstrated similar efficacy.
• N-(2-((4- hydroxyphenyl ) amino ) pyrid-3 -yl) -4-methoxybenzenesulfonamide demonstrated greater than additive responses with N-(2-((4- 580 hydroxyphenyl) amino) pyrid-3 -yl) -4-methoxybenzenesulfonamide.
• tumor cells derived from serially passaged tumor fragments were inoculated 585 subcutaneously in female nude mice on day 0. On day 10, mice bearing established tumors were size matched and grouped at about 231 mm 3 .
• docetaxel at 33.3 mg/kg/day N-(2-((4- hydroxyphenyl) amino) pyrid-3-yl) -4-methoxybenzenesulfonamide
• mice 600 the efficacy of N- (2- ( (4-hydroxyphenyl) ammo) pyrid-3-yl) -4- methoxybenzenesulfonamide in the HT-29 Flank Xenograft model, tumor cells derived from serially passaged tumor fragments were inoculated subcutaneously in female nude mice on day 0. On day 10, mice bearing established tumors were size matched
• 660 is defined as life threatening.

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• 2004
  • 2004-06-01 EP EP04753737A patent/EP1644008B1/en not_active Expired - Lifetime
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