WO2004101788A3 - Bibliotheques de petits arn interferants, procedes de synthese et d'utilisation - Google Patents

Bibliotheques de petits arn interferants, procedes de synthese et d'utilisation Download PDF

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Publication number
WO2004101788A3
WO2004101788A3 PCT/US2004/014494 US2004014494W WO2004101788A3 WO 2004101788 A3 WO2004101788 A3 WO 2004101788A3 US 2004014494 W US2004014494 W US 2004014494W WO 2004101788 A3 WO2004101788 A3 WO 2004101788A3
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Prior art keywords
sirna
subpopulation
population
cells
libraries
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PCT/US2004/014494
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English (en)
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WO2004101788A2 (fr
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Mark Nichols
Richard Steinman
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Univ Pittsburgh
Mark Nichols
Richard Steinman
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Application filed by Univ Pittsburgh, Mark Nichols, Richard Steinman filed Critical Univ Pittsburgh
Priority to CA002523785A priority Critical patent/CA2523785A1/fr
Priority to EP04760959A priority patent/EP1623032A2/fr
Priority to AU2004239303A priority patent/AU2004239303A1/en
Priority to JP2006532898A priority patent/JP2007502129A/ja
Publication of WO2004101788A2 publication Critical patent/WO2004101788A2/fr
Publication of WO2004101788A3 publication Critical patent/WO2004101788A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1138—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against receptors or cell surface proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A61P35/04—Antineoplastic agents specific for metastasis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C12N2310/111—Antisense spanning the whole gene, or a large part of it
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/30—Production chemically synthesised
    • C12N2330/31—Libraries, arrays

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oncology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Virology (AREA)
  • Communicable Diseases (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

L'invention concerne, dans un aspect, une bibliothèque (de codage) d'ARNi aléatoires ou semi-aléatoires. Dans un autre aspect, elle concerne des méthodes de construction de bibliothèques (de codage) d'ARNi aléatoires ou semi-aléatoires. Dans un autre aspect, l'invention concerne des systèmes de vecteurs utilisables dans la construction de bibliothèques d'ARNi et/ou pouvant exprimer des ARNi individuels ou des bibliothèques d'ARNi, tant de manière constitutive que de façon inductible. Dans un autre aspect, l'invention concerne une méthode d'utilisation d'une bibliothèque d'ARNi. La bibliothèque d'ARNi est introduite dans la population de cellules. La population de cellules est ensuite soumise à un processus de sélection d'une sous-population de cellules présentant une propriété comportementale, biochimique, chimique, fonctionnelle, moléculaire, morphologique, phénotypique ou physique différente de celle du reste de la population. A l'issue du processus de sélection, la sous-population de cellules peut être isolée, analysée et/ou clonée à loisir. Une telle analyse de la sous-population peut consister à identifier et séquencer les espèces d'ARNi responsables pour les différentes propriétés de la sous-population relativement au reste de la population. En variante, la sous-population peut subséquemment être analysée grâce à des dosages génomiques, protéomiques et/ou cellomiques. Lorsque de tels dosages génomiques, protéomiques et/ou cellomiques sont mis en oeuvre, la méthode de l'invention peut produire plusieurs produits bio-informatiques utiles. Des ARNi spécifiques identifiés par ce processus peuvent présenter un intérêt thérapeutique direct.
PCT/US2004/014494 2003-05-09 2004-05-10 Bibliotheques de petits arn interferants, procedes de synthese et d'utilisation WO2004101788A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA002523785A CA2523785A1 (fr) 2003-05-09 2004-05-10 Bibliotheques de petits arn interferants, procedes de synthese et d'utilisation
EP04760959A EP1623032A2 (fr) 2003-05-09 2004-05-10 Bibliotheques de petits arn interferants, procedes de synthese et d'utilisation
AU2004239303A AU2004239303A1 (en) 2003-05-09 2004-05-10 Small interfering RNA libraries and methods of synthesis and use
JP2006532898A JP2007502129A (ja) 2003-05-09 2004-05-10 短鎖干渉ｒｎａライブラリーならびに合成および使用の方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US46916903P 2003-05-09 2003-05-09
US60/469,169 2003-05-09

Publications (2)

Publication Number Publication Date
WO2004101788A2 WO2004101788A2 (fr) 2004-11-25
WO2004101788A3 true WO2004101788A3 (fr) 2005-06-30

Family

ID=33452262

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/014494 WO2004101788A2 (fr) 2003-05-09 2004-05-10 Bibliotheques de petits arn interferants, procedes de synthese et d'utilisation

Country Status (6)

Country Link
US (1) US7524653B2 (fr)
EP (1) EP1623032A2 (fr)
JP (1) JP2007502129A (fr)
AU (1) AU2004239303A1 (fr)
CA (1) CA2523785A1 (fr)
WO (1) WO2004101788A2 (fr)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4747245B2 (ja) * 2003-12-31 2011-08-17 謙造 廣瀬 ＲＮＡｉライブラリーの酵素的構築方法
US20090004703A1 (en) * 2004-05-28 2009-01-01 Mologen Ag Method for the Production of Suitable Dna Constructs for Specific Inhibition of Gene Expression by Rna Interference
DE102004063265B4 (de) * 2004-12-29 2012-02-02 Biontech Ag Verfahren zur Bestimmung der Funktion von Nukleinsäuresequenzen und den dadurch kodierten Expressionsprodukten
WO2006130976A1 (fr) * 2005-06-10 2006-12-14 National Research Council Of Canada Arn interferents, procedes d'elaboration et utilisation
AU2014202015B2 (en) * 2006-03-07 2016-06-02 The Trustees Of The University Of Pennsylvania Random RNAi libraries, methods of generating same, and screening methods utilizing same
EP2543738A3 (fr) 2006-03-07 2013-04-24 The Trustees Of The University Of Pennsylvania Bibliothèques ARNi aléatoires, leurs procédés de génération et procédés de criblage les utilisant
WO2009071722A1 (fr) * 2007-12-07 2009-06-11 Newbiotechnic, S.A. PROCÉDÉS ET TROUSSES POUR LA PRÉPARATION DE GÉNOTHÈQUES D'ARNsi SPÉCIFIQUES D'UN TRANSCRIPTOME PAR TRANSCRIPTION CONVERGENTE
US20100297756A1 (en) * 2007-12-13 2010-11-25 Abdennajj Adib Means for delivery of nucleic acids active for gene silencing using synthetic polymers
US8309791B2 (en) * 2008-07-16 2012-11-13 Recombinectics, Inc. Method for producing a transgenic pig using a hyper-methylated transposon
US8278048B2 (en) 2008-08-15 2012-10-02 Cascade Biosystems, Inc. Detecting nucleic acid
US8551701B2 (en) 2010-02-15 2013-10-08 Cascade Biosystems, Inc. Methods and materials for detecting genetic or epigenetic elements
WO2011100747A1 (fr) 2010-02-15 2011-08-18 Cascade Biosystems, Inc. Procédés et matériaux permettant de détecter des produits alimentaires contaminés
US8597886B2 (en) 2010-02-15 2013-12-03 Cascade Biosystems, Inc. Methods and materials for detecting viral or microbial infections
EP2536847B1 (fr) 2010-02-15 2018-08-08 Cascade Biosystems, Inc. Procédés et matériaux pour l'évaluation de l'expression d'arn
CA2920608C (fr) * 2013-05-28 2018-07-24 Five3 Genomics, Llc Reseaux de reactions a un medicament paradigmatiques
US11390882B2 (en) * 2019-03-12 2022-07-19 Varigen Biosciences Corporation Expression vector

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0501914A1 (fr) * 1991-02-25 1992-09-02 Ciba-Geigy Ag Vecteurs de levure améliorés
WO2003020931A2 (fr) * 2001-09-01 2003-03-13 Galapagos Genomics N.V. Dosage biologique de choc a interference arn de courte duree et constructions

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69839501D1 (de) 1997-02-27 2008-06-26 Cellomics Inc System für Screening biologischer Zellen
US6506559B1 (en) 1997-12-23 2003-01-14 Carnegie Institute Of Washington Genetic inhibition by double-stranded RNA
AUPP249298A0 (en) 1998-03-20 1998-04-23 Ag-Gene Australia Limited Synthetic genes and genetic constructs comprising same I
HU230458B1 (hu) 2000-12-01 2016-07-28 Europäisches Laboratorium für Molekularbiologie (EMBL) Az RNS interferenciát közvetítő kis RNS molekulák
JP2005502383A (ja) 2001-09-27 2005-01-27 ティモシー アルバート ホルトン 核酸ライブラリーを作製する方法
AU2003211058A1 (en) 2002-02-20 2003-09-09 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED TARGET DISCOVERY AND TARGET VALIDATION USING SHORT INTERFERING NUCLEIC ACID (siNA)
CA2493251A1 (fr) 2002-07-24 2004-01-29 Immusol Incorporated Systeme a un seul promoteur pour la production de cassettes d'expression d'arnsi et genotheque d'expression reposant sur l'utilisation d'un lieur en epingle a cheveux d'amorce de polymerase
CA2493564A1 (fr) 2002-07-24 2004-01-29 Immusol, Inc. Nouvelles banques genomiques de petits fragments d'arn interferents et procedes de production et d'utilisation de ces banques
US20040224328A1 (en) 2003-01-15 2004-11-11 Hans Prydz siRNA screening method
US20040224405A1 (en) 2003-05-06 2004-11-11 Dharmacon Inc. siRNA induced systemic gene silencing in mammalian systems
US20050130184A1 (en) 2003-07-18 2005-06-16 University Of Massachusetts Enhanced promoters for synthesis of small hairpin RNA

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0501914A1 (fr) * 1991-02-25 1992-09-02 Ciba-Geigy Ag Vecteurs de levure améliorés
WO2003020931A2 (fr) * 2001-09-01 2003-03-13 Galapagos Genomics N.V. Dosage biologique de choc a interference arn de courte duree et constructions

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
HANNON G J: "RNA interference", NATURE, MACMILLAN JOURNALS LTD. LONDON, GB, vol. 418, 11 July 2002 (2002-07-11), pages 244 - 251, XP002979088, ISSN: 0028-0836 *
KANO M ET AL: "CRE-IOXP-MEDIATED DNA FLIP-FLOP IN MAMMALIAN CELLS LEADING TO ALTERNATE EXPRESSION OF RETROVIRALLY TRANSDUCED GENES", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC. ORLANDO, FL, US, vol. 248, 1998, pages 806 - 811, XP000827972, ISSN: 0006-291X *
KAWASAKI HIROAKI ET AL: "siRNAs generated by recombinant human Dicer induce specific and significant but target site-independent gene silencing in human cells.", NUCLEIC ACIDS RESEARCH. 1 FEB 2003, vol. 31, no. 3, 1 February 2003 (2003-02-01), pages 981 - 987, XP002323761, ISSN: 1362-4962 *
MIYAGISHI M ET AL: "U6 PROMOTER-DRIVEN SIRNAS WITH FOUR URIDINE 3' OVERHANGS EFFICIENTLY SUPPRESS TARGETED GENE EXPRESSION IN MAMMALIAN CELLS", NATURE BIOTECHNOLOGY, NATURE PUBLISHING, US, vol. 19, no. 5, May 2002 (2002-05-01), pages 497 - 500, XP001153719, ISSN: 1087-0156 *
SEKTAS M ET AL: "TIGHTLY CONTROLLED TWO-STAGE EXPRESSION VECTORS EMPLOYING THE FLP/FRT-MEDIATED INVERSION OF CLONED GENES", MOLECULAR BIOTECHNOLOGY, TOTOWA, NJ, US, vol. 9, no. 1, February 1998 (1998-02-01), pages 17 - 24, XP001034234, ISSN: 1073-6085 *
SEN G ET AL: "Restriction enzyme-generated siRNA (REGS) vectors and libraries", NATURE GENETICS 2004 UNITED STATES, vol. 36, no. 2, 2004, pages 183 - 189, XP002323763, ISSN: 1061-4036 *
SHIRANE DAISUKE ET AL: "Enzymatic production of RNAi libraries from cDNAs.", NATURE GENETICS, vol. 36, no. 2, February 2004 (2004-02-01), pages 190 - 196, XP002323762, ISSN: 1061-4036 *
TRINH K R ET AL: "Site-specific and directional gene replacement mediated by Cre recombinase", JOURNAL OF IMMUNOLOGICAL METHODS, ELSEVIER SCIENCE PUBLISHERS B.V.,AMSTERDAM, NL, vol. 244, no. 1-2, 20 October 2000 (2000-10-20), pages 185 - 193, XP004218458, ISSN: 0022-1759 *
YOON Y G ET AL: "Cre/loxP-mediated in vivo excision of large segments from yeast genome and their amplification based on the 2mum plasmid-derived system", GENE, ELSEVIER BIOMEDICAL PRESS. AMSTERDAM, NL, vol. 223, no. 1-2, 26 November 1998 (1998-11-26), pages 67 - 76, XP004153578, ISSN: 0378-1119 *

Also Published As

Publication number Publication date
US20070166720A1 (en) 2007-07-19
JP2007502129A (ja) 2007-02-08
CA2523785A1 (fr) 2004-11-25
US7524653B2 (en) 2009-04-28
EP1623032A2 (fr) 2006-02-08
WO2004101788A2 (fr) 2004-11-25
AU2004239303A1 (en) 2004-11-25

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