WO2004084654A1 - A process for the encapsulation of garcinia extract - Google Patents
A process for the encapsulation of garcinia extract Download PDFInfo
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- WO2004084654A1 WO2004084654A1 PCT/IN2003/000086 IN0300086W WO2004084654A1 WO 2004084654 A1 WO2004084654 A1 WO 2004084654A1 IN 0300086 W IN0300086 W IN 0300086W WO 2004084654 A1 WO2004084654 A1 WO 2004084654A1
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- Prior art keywords
- extract
- garcinia
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- powder
- fruits
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- 239000000284 extract Substances 0.000 title claims abstract description 45
- 238000000034 method Methods 0.000 title claims abstract description 38
- 235000000885 Garcinia xanthochymus Nutrition 0.000 title claims abstract description 34
- 238000005538 encapsulation Methods 0.000 title claims abstract description 12
- 244000119461 Garcinia xanthochymus Species 0.000 title 1
- 241000593508 Garcinia Species 0.000 claims abstract description 33
- 239000000843 powder Substances 0.000 claims abstract description 27
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 21
- 108010046377 Whey Proteins Proteins 0.000 claims abstract description 16
- 102000007544 Whey Proteins Human genes 0.000 claims abstract description 16
- 235000021119 whey protein Nutrition 0.000 claims abstract description 16
- 241000283690 Bos taurus Species 0.000 claims abstract description 12
- 239000008367 deionised water Substances 0.000 claims abstract description 10
- 239000004744 fabric Substances 0.000 claims abstract description 8
- 238000001694 spray drying Methods 0.000 claims abstract description 6
- 238000001914 filtration Methods 0.000 claims abstract description 4
- 238000002156 mixing Methods 0.000 claims abstract description 4
- 239000000203 mixture Substances 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- ZMJBYMUCKBYSCP-UHFFFAOYSA-N Hydroxycitric acid Chemical compound OC(=O)C(O)C(O)(C(O)=O)CC(O)=O ZMJBYMUCKBYSCP-UHFFFAOYSA-N 0.000 claims description 10
- 241001051053 Garcinia cowa Species 0.000 claims description 9
- 241001627228 Garcinia pedunculata Species 0.000 claims description 6
- 229940089491 hydroxycitric acid Drugs 0.000 claims description 6
- 241000196324 Embryophyta Species 0.000 claims description 4
- 235000020776 essential amino acid Nutrition 0.000 claims description 3
- 239000003797 essential amino acid Substances 0.000 claims description 3
- 238000000605 extraction Methods 0.000 claims description 3
- 239000006286 aqueous extract Substances 0.000 claims description 2
- 241000894007 species Species 0.000 abstract description 6
- 239000007921 spray Substances 0.000 description 8
- 238000004128 high performance liquid chromatography Methods 0.000 description 6
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 4
- 229910052782 aluminium Inorganic materials 0.000 description 4
- 239000000706 filtrate Substances 0.000 description 4
- 235000013305 food Nutrition 0.000 description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 3
- 239000012467 final product Substances 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- -1 HCA lactone Chemical class 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 159000000007 calcium salts Chemical class 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- VYYWVGGAJXBBCA-YIRLFHOGSA-K tripotassium;(1s,2s)-1,2-dihydroxypropane-1,2,3-tricarboxylate Chemical compound [K+].[K+].[K+].[O-]C(=O)[C@@H](O)[C@](O)(C([O-])=O)CC([O-])=O VYYWVGGAJXBBCA-YIRLFHOGSA-K 0.000 description 2
- 239000003643 water by type Substances 0.000 description 2
- 230000004580 weight loss Effects 0.000 description 2
- GMVPRGQOIOIIMI-DODZYUBVSA-N 7-[(1R,2R,3R)-3-hydroxy-2-[(3S)-3-hydroxyoct-1-enyl]-5-oxocyclopentyl]heptanoic acid Chemical compound CCCCC[C@H](O)C=C[C@H]1[C@H](O)CC(=O)[C@@H]1CCCCCCC(O)=O GMVPRGQOIOIIMI-DODZYUBVSA-N 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 238000009010 Bradford assay Methods 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241000546193 Clusiaceae Species 0.000 description 1
- 244000245602 Garcinia atroviridis Species 0.000 description 1
- 229920002527 Glycogen Polymers 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- YVNQAIFQFWTPLQ-UHFFFAOYSA-O [4-[[4-(4-ethoxyanilino)phenyl]-[4-[ethyl-[(3-sulfophenyl)methyl]amino]-2-methylphenyl]methylidene]-3-methylcyclohexa-2,5-dien-1-ylidene]-ethyl-[(3-sulfophenyl)methyl]azanium Chemical compound C1=CC(OCC)=CC=C1NC1=CC=C(C(=C2C(=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S(O)(=O)=O)C)C=2C(=CC(=CC=2)N(CC)CC=2C=C(C=CC=2)S(O)(=O)=O)C)C=C1 YVNQAIFQFWTPLQ-UHFFFAOYSA-O 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 235000019631 acid taste sensations Nutrition 0.000 description 1
- 125000005233 alkylalcohol group Chemical group 0.000 description 1
- 230000036528 appetite Effects 0.000 description 1
- 235000019789 appetite Nutrition 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 230000005961 cardioprotection Effects 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 238000004042 decolorization Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 235000015872 dietary supplement Nutrition 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000004136 fatty acid synthesis Effects 0.000 description 1
- 229940124600 folk medicine Drugs 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 235000012631 food intake Nutrition 0.000 description 1
- 229940096919 glycogen Drugs 0.000 description 1
- 230000007407 health benefit Effects 0.000 description 1
- 230000002440 hepatic effect Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 239000003456 ion exchange resin Substances 0.000 description 1
- 229920003303 ion-exchange polymer Polymers 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 230000004132 lipogenesis Effects 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- 239000000825 pharmaceutical preparation Substances 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- XAEFZNCEHLXOMS-UHFFFAOYSA-M potassium benzoate Chemical compound [K+].[O-]C(=O)C1=CC=CC=C1 XAEFZNCEHLXOMS-UHFFFAOYSA-M 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000001117 sulphuric acid Substances 0.000 description 1
- 235000011149 sulphuric acid Nutrition 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
- 238000010977 unit operation Methods 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
- 230000037221 weight management Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING OR TREATMENT THEREOF
- A23C21/00—Whey; Whey preparations
- A23C21/08—Whey; Whey preparations containing other organic additives, e.g. vegetable or animal products
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
- A23L27/20—Synthetic spices, flavouring agents or condiments
- A23L27/206—Dairy flavours
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
- A23L27/70—Fixation, conservation, or encapsulation of flavouring agents
- A23L27/72—Encapsulation
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P10/00—Shaping or working of foodstuffs characterised by the products
- A23P10/30—Encapsulation of particles, e.g. foodstuff additives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/04—Anorexiants; Antiobesity agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Definitions
- the present invention relates to a process for the encapsulation of Garcinia extract.
- the present invention particularly relates to a process of obtaining Garcinia extract in powder form by encapsulation using whey protein.
- HCA Hexadroxcitic acid
- Allison et al. (Crit.Rev.Food Sci.Nutr.2001, 41,1-28) has reviewed the use of HCA as one of the alternative treatments for weight loss.
- the derivatives of HCA have been incorporated into many pharmaceutical preparations in combination with other ingredients for the purpose of enhancing weight loss, cardioprotection, correcting the conditions of lipid abnormalities and endurance (Jena et al., 2002, J. Agric. Food Chemistry, 50,10-22). So far, HCA has been found in the fruits of certain species of Garcinia, which includes G.
- HCA has been proven to effectively curb appetite, suppress food intake, increase the rates of hepatic glycogen synthesis, reduce fatty acid synthesis and lipogenesis and decrease body-weight gain.
- Garcinia (Family: Guttiferae) is a large genus of polygamous trees or shrubs, distributed in the tropical Asia, Africa and Polynesia. It consists of 180 species, out of which about 30 species are found in India. G. pedunculata and G. cowa are grown in Northeastern parts of India and Andaman Islands. In Assam, G. cowa is often cultivated in homesteads for its acid fruits (The Wealth of India, 1956). The fruits from both the species of Garcinia are not palatable due to their strong acid taste. In Assam, the sun-dried slices of the fruits are used for culinary purposes and as folk medicine.
- the main object of the present invention is to develop a novel process for the encapsulation of Garcinia extract.
- Another object of the present invention is to develop a process for the encapsulation of HCA in powder form with whey protein to get stable and non- hygroscopic.
- Still another object of present invention is to get encapsulated HCA powder.
- the present invention provides a novel process for the encapsulation of Garcinia extract powder, which comprises (a) collecting the fruits from the species of Garcinia, (b) cutting the rinds of the fruits manually, (c) extracting with de-ionized water at a volume ratio of 1:4 for a period of 15-35 min at 110-130°C, (d) filtering the extract using filter cloth, (e) mixing of the Garcinia extract with 5-20 % (w/w) whey protein and (f) spray drying the above mixture to obtain the product in powder form.
- the process of encapsulation of Garcinia extract comprising steps of: a) collecting the fruits of Garcinia pedunculata/ Garcinia cowa, b) cutting rind fruits of the plant of step (a), c) extracting the cut rind fruits of step (b) with de-ionized water for a time period of 20 to 30 minutes at a temperature range of 100-135° C, d) filtering the water extract of step (c) through a filter cloth to obtain a water extract, e) mixing the water extract of step (d) with Whey protein, and f) spray drying the mixture of step (e) to obtain the encapsulated Garcinia extract powder.
- the size of the cut rind fruits ranges between 5 x 7 mm to 9 x 11 mm.
- the ratio of the plant to de-ionized water used is in the range of 1:4 and the extraction is performed at a temperature in the range of 115°C to 125°C.
- the weight/weight ratio of water extract of Garcinia: Whey protein is in the range of 85:15 to 90:10.
- the spray drying of the water extract is performed maintaining an inlet temperature in the range of 140 to 150° C and an outlet temperature in the range of 90° to 95° C.
- the feed rate of water extract is in the range of 80 ml to
- the hydroxycitric acid is obtained form the aqueous extract of Garcinia pedunculate or Garcinia Cowa.
- the hydroxycitric content of encapsulated powder of extract is in the range of 53-62% on dry basis.
- the Whey protein content of the encapsulated powder of extract is in the range of 30 - 44 % determined by Bradfort method.
- One more embodiment of the invention provides a process in which the encapsulated powder of Garcinia extract contains hydroxycitric acid in the native form and it is stable and non-hygroscopic.
- the encapsulated powder of Garcinia extract possesses better bioavailability. It also provides essential amino acids.
- rinds of G. pedunculata / G.cowa were cut into small pieces and extracted with de-ionized water.
- the above extract was filtered through filter cloth.
- the entire filtrate was mixed with whey protein and fed to the spray dryer with a known feed rate.
- Spray dryer was maintained at desired inlet and outlet temperatures. Powder was collected from the bottom of the dryer in an aluminum pack and immediately sealed.
- the purity of the preparation was analyzed by HPLC as described by Jayaprakasha, G.K. and Sakariah, K.K. (J. Liquid Chromatography & Related Technologies, 23, 915-923, 2000).
- Encapsulated hydroxycitric acid powder (O.lg) quantity was dissolved in 5ml water and made up to lOOml with water and filtered.
- the high performance liquid chromatographic system consisted of a Hewlett Packard HPLC model HP 1100 Series (Hewlett-Packard, CA, USA), fitted with a Waters ⁇ -BondapackTM (Waters Corporation, Milford, MA, USA) C 18 column (250 x 4.6 mm ID).
- the injection system (Rheodyne) used was 20 ⁇ l sample loop. Detection was done by a HP 1100 series variable wavelength detector at wavelength of 210 nm.
- the elution was carried out with 8 mM sulphuric acid and flow rate was 1.0 ml/min under isocratic condition.
- a known volume (lO ⁇ l) of the samples was injected on to the HPLC and the concentration of HCA was obtained directly from the peak area and by application of the dilution factor.
- the HCA concentration of the sample was expressed as g/100 g of sample.
- the purity of hydroxycitric acid was 20-23% and HCA lactone was 30-39% (w/w).
- Protein concentration was determined by Bradford method (1976) using coomassie brilliant blue G-250 using bovine serum albumin as standard. The protein content was found to be 30-44%.
- Encapsulation of HCA is achieved in its native form and not as derivative (salt).
- HCA powder obtained is in shelf stable, non-hygroscopic.
- Hydroxycitrate content was 53-62 % (w/w)
- the Garcinia extract powder obtained from the present process is highly soluble in water.
- the final product with whey protein has additional health benefits from the angle of essential amino acid content.
- Example-1 is given by way of illustration of the present invention and should not be construed to limit the scope of the present invention.
- Example-3 Fruit rinds of G. pedunculata in lOOgm quantity were cut into small pieces and extracting with 1 liter of de-ionized water for a period of 50 min at 120°C using autoclaving. The extract was filtered through filter cloth. The entire filtrate (1-kg) was mixed with 15g of whey protein and fed through the spray dryer with the feed rate of 95 ml/min. The inlet and outlet temperature of spray dryer was maintained at 155°C and 94°C, respectively. Powder was collected from the bottom of the dryer in an aluminum pack and immediately sealed. The HCA content was determined by HPLC method and the hydroxycitrate content was 62% (w/w). The protein content was found to be 31%. The main advantages of the present invention are 1.
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Abstract
The present invention relates to a novel process for the encapsulation of Garcinia extract powder, which comprises (a) collecting the fruits from the species of Garcinia, (b) cutting the rinds of the fruits manually, (c) extracting with de-ionized water at a volume ratio of 1:4 for a period of 15-35 min at 110-130°C, (d) filtering the extract using filter cloth, (e) mixing of the Garcinia extract with 5-20 % (w/w) whey protein and (f) spray drying the above mixture to obtain the product in powder form.
Description
A PROCESS FOR THE ENCAPSULATION OF GARCINIA EXTRACT Field of the invention
The present invention relates to a process for the encapsulation of Garcinia extract. The present invention particularly relates to a process of obtaining Garcinia extract in powder form by encapsulation using whey protein. Background and Prior art references
As dietary supplement, (-)-Hydroxcitic acid (HCA) is an effective addition to any weight management program. Allison et al. (Crit.Rev.Food Sci.Nutr.2001, 41,1-28) has reviewed the use of HCA as one of the alternative treatments for weight loss. The derivatives of HCA have been incorporated into many pharmaceutical preparations in combination with other ingredients for the purpose of enhancing weight loss, cardioprotection, correcting the conditions of lipid abnormalities and endurance (Jena et al., 2002, J. Agric. Food Chemistry, 50,10-22). So far, HCA has been found in the fruits of certain species of Garcinia, which includes G. cambogia, G indica, G.atroviridis and G cowa (Lewis, Y.S. 1969, Methods in enzymology, 13, 613-623; Jena et al, 2002, J. Agric. Food Chemistry, 50,10-22). The chemistry and biochemistry of HCA has been discussed recently (Jena et. al., 2002, J. Agric. Food Chemistry, 50,10-22). During extensive animal studies, HCA has been proven to effectively curb appetite, suppress food intake, increase the rates of hepatic glycogen synthesis, reduce fatty acid synthesis and lipogenesis and decrease body-weight gain.
Garcinia (Family: Guttiferae) is a large genus of polygamous trees or shrubs, distributed in the tropical Asia, Africa and Polynesia. It consists of 180 species, out of which about 30 species are found in India. G. pedunculata and G. cowa are grown in Northeastern parts of India and Andaman Islands. In Assam, G. cowa is often cultivated in homesteads for its acid fruits (The Wealth of India, 1956). The fruits from both the species of Garcinia are not palatable due to their strong acid taste. In Assam, the sun-dried slices of the fruits are used for culinary purposes and as folk medicine.
Reference may be made to the commercial samples of Garcinia cambogia extracts, where the HCA is present as its calcium salt (Sawada, et al, 1997, Nihon yukagaka kaishi, vol, 1467-1474). However, the excess calcium reduces the solubility and subsequently bioavailability when it is compared to the liquid extract.
Another reference may be made to the Ashok kumar, Ravindranath, B., and Balasubramamanvam (US patent # 656314, 1996). This process involves water extraction, followed by passing through ion exchange resins and decolorisation by using activated
charcoal and concentration. The main drawback of this method is the process involves too many unit operations for obtaining the final product.
Reference may be made to Majeed et al. (Majeed, M., Badmaev, V and Rajendran, R. US Patent, no 5,783,603, 1998), wherein the preparation of potassium hydroxycitrate from Garcinia fruit was reported. It involves the extraction of Garcinia fruit using alkyl alcohol, the extract was treated with potassium hydroxide and refluxed to form potassium hydroxycitrate precipitate. The main drawback of this method is the potassium salt is hygroscopic. Further, HCA is not available in natural state, limiting its bioavailability and versatile applicability for pharmaceutical purposes. It may be noted that all these references are mentioned above HCA derivative preparations. However, there is no report on the encapsulation of HCA in its native (not as derivatives) form. Objects of the invention
The main object of the present invention is to develop a novel process for the encapsulation of Garcinia extract.
Another object of the present invention is to develop a process for the encapsulation of HCA in powder form with whey protein to get stable and non- hygroscopic.
Still another object of present invention is to get encapsulated HCA powder. Summary of the Invention
Accordingly, the present invention provides a novel process for the encapsulation of Garcinia extract powder, which comprises (a) collecting the fruits from the species of Garcinia, (b) cutting the rinds of the fruits manually, (c) extracting with de-ionized water at a volume ratio of 1:4 for a period of 15-35 min at 110-130°C, (d) filtering the extract using filter cloth, (e) mixing of the Garcinia extract with 5-20 % (w/w) whey protein and (f) spray drying the above mixture to obtain the product in powder form. Detailed Description of the Invention
In accordance to the objectives of the present invention, the process of encapsulation of Garcinia extract comprising steps of: a) collecting the fruits of Garcinia pedunculata/ Garcinia cowa, b) cutting rind fruits of the plant of step (a), c) extracting the cut rind fruits of step (b) with de-ionized water for a time period of 20 to 30 minutes at a temperature range of 100-135° C,
d) filtering the water extract of step (c) through a filter cloth to obtain a water extract, e) mixing the water extract of step (d) with Whey protein, and f) spray drying the mixture of step (e) to obtain the encapsulated Garcinia extract powder. In an embodiment of the invention provides a process, wherein in step (b) the size of the cut rind fruits ranges between 5 x 7 mm to 9 x 11 mm.
One more embodiment, the ratio of the plant to de-ionized water used, is in the range of 1:4 and the extraction is performed at a temperature in the range of 115°C to 125°C. Another embodiment, wherein in step (e) the weight/weight ratio of water extract of Garcinia: Whey protein is in the range of 85:15 to 90:10.
Still another embodiment, the spray drying of the water extract is performed maintaining an inlet temperature in the range of 140 to 150° C and an outlet temperature in the range of 90° to 95° C. Still another embodiment, the feed rate of water extract is in the range of 80 ml to
90 ml per minute.
Yet another embodiment, the hydroxycitric acid is obtained form the aqueous extract of Garcinia pedunculate or Garcinia Cowa.
Yet another embodiment, the hydroxycitric content of encapsulated powder of extract is in the range of 53-62% on dry basis.
Yet another embodiment, the Whey protein content of the encapsulated powder of extract is in the range of 30 - 44 % determined by Bradfort method.
One more embodiment of the invention provides a process in which the encapsulated powder of Garcinia extract contains hydroxycitric acid in the native form and it is stable and non-hygroscopic.
Another embodiment, the encapsulated powder of Garcinia extract possesses better bioavailability. It also provides essential amino acids.
In the present invention, rinds of G. pedunculata / G.cowa were cut into small pieces and extracted with de-ionized water. The above extract was filtered through filter cloth. The entire filtrate was mixed with whey protein and fed to the spray dryer with a known feed rate. Spray dryer was maintained at desired inlet and outlet temperatures. Powder was collected from the bottom of the dryer in an aluminum pack and immediately sealed.
The purity of the preparation was analyzed by HPLC as described by Jayaprakasha, G.K. and Sakariah, K.K. (J. Liquid Chromatography & Related Technologies, 23, 915-923, 2000). Encapsulated hydroxycitric acid powder (O.lg) quantity was dissolved in 5ml water and made up to lOOml with water and filtered. The high performance liquid chromatographic system consisted of a Hewlett Packard HPLC model HP 1100 Series (Hewlett-Packard, CA, USA), fitted with a Waters μ-Bondapack™ (Waters Corporation, Milford, MA, USA) C18 column (250 x 4.6 mm ID). The injection system (Rheodyne) used was 20 μl sample loop. Detection was done by a HP 1100 series variable wavelength detector at wavelength of 210 nm. The elution was carried out with 8 mM sulphuric acid and flow rate was 1.0 ml/min under isocratic condition. A known volume (lOμl) of the samples was injected on to the HPLC and the concentration of HCA was obtained directly from the peak area and by application of the dilution factor. The HCA concentration of the sample was expressed as g/100 g of sample. The purity of hydroxycitric acid was 20-23% and HCA lactone was 30-39% (w/w). Protein concentration was determined by Bradford method (1976) using coomassie brilliant blue G-250 using bovine serum albumin as standard. The protein content was found to be 30-44%. Product specification
(a). Color - cream white
(b). Moisture content - 8-15 % (c). Free HCA - 20-23 %
(d). HCA lactone - 30-39 %
(e). Whey protein content - 30-44%
(f). Solubility - Highly soluble in water and methanol
(g). Storage Stability - Store under humid free condition The novelty of the present invention is
1. Encapsulation of HCA is achieved in its native form and not as derivative (salt).
2. HCA powder obtained is in shelf stable, non-hygroscopic.
3. Hydroxycitrate content was 53-62 % (w/w)
4. The Garcinia extract powder obtained from the present process is highly soluble in water.
5. The final product with whey protein has additional health benefits from the angle of essential amino acid content.
The following examples are given by way of illustration of the present invention and should not be construed to limit the scope of the present invention.
Example-1
Fruit rinds of G. pedunculata in 500g quantity were cut into small pieces and extracted with 1.5 liters of de-ionized water for a period of 20 min at 120°C. The above extract was filtered through filter cloth. The filtrate (1.5 kg) was mixed with 150g whey protein and fed through the spray dryer with the feed rate of 75ml/min. The inlet and outlet temperature of spray dryer was maintained at 135°C and 95 °C. HCA Powder was collected from the bottom of the dryer in an aluminum pack and sealed immediately. The HCA content was determined by HPLC method and the hydroxycitrate content was 61% (w/w). The protein content was found to be 30%. Example-2
Fruit rinds of G. cowa in lOOOg quantity were cut into small pieces and extracted with 3 liters of de-ionized water for a period of 30 min at 130 psi using autoclaving. The extract was filtered through filter cloth. The entire filtrate (3kg) was mixed with 300 g whey protein and fed through the spray dryer with the feed rate of 80ml/min. The inlet and outlet temperature of spray dryer was maintained at 150°C and 90°C,respectively. HCA powder was collected from the bottom of the dryer in an aluminum pack and sealed immediately. The HCA content was determined by HPLC method and the hydroxycitrate content was 53% (w/w). The protein content was found to be 34%.
Example-3 Fruit rinds of G. pedunculata in lOOgm quantity were cut into small pieces and extracting with 1 liter of de-ionized water for a period of 50 min at 120°C using autoclaving. The extract was filtered through filter cloth. The entire filtrate (1-kg) was mixed with 15g of whey protein and fed through the spray dryer with the feed rate of 95 ml/min. The inlet and outlet temperature of spray dryer was maintained at 155°C and 94°C, respectively. Powder was collected from the bottom of the dryer in an aluminum pack and immediately sealed. The HCA content was determined by HPLC method and the hydroxycitrate content was 62% (w/w). The protein content was found to be 31%. The main advantages of the present invention are 1. This is a simple process for obtaining the encapsulated Garcinia powder. 2. This encapsulated of Garcinia powder is in its native form without being any of its derivatives like sodium, potassium and calcium salts. Hence, it will have better bioavailability. 3. Recovery of final product is high, because it is less hygroscopic.
Claims
1. A process for the encapsulation of Garcinia extract the said process comprising steps of: a) collecting the fruits of Garcinia pedunculata Garcinia cowa, b) cutting rind fruits of the plant of step (a), c) extracting the cut rind fruits of step (b) with de-ionized water for a time period of 20 to 30 minutes at a temperature range of 100-135° C, d) filtering the water extract of step (c) through a filter cloth to obtain a water extract, e) mixing the water extract of step (d) with Whey protein, and f) spray drying the mixture of step (e) to obtain the encapsulated Garcinia extract powder.
2. A process as claimed in claim 1, wherein in step (b) the size of the cut rind fruits ranges between 5 x 7 mm to 9 x 11 mm.
3. A process as claimed in claim 1, wherein in step (c) the ratio of the plant to de-ionized water used, is in the range of 1 :4.
4. A process as claimed in claim 1, wherein in step (c) the extraction is performed at a temperature in the range of 115° C to 125°C.
5. A process as claimed in claim 1 wherein in step (e), the weight/weight ratio of water extract of Garcinia: Whey protein is in the range of 85:15 to 90:10.
6. A process as claimed in claim 1, wherein in step (f) the spray drying of the water extract is performed maintaining an inlet temperature in the range of 140 to 150° C and an outlet temperature in the range of 90° to 95°C.
7. A process as claimed in claim 1, wherein in step (f) the feed rate of water extract is in the range of 80 ml to 90 ml per minute.
8. A process as claimed in claim 1, wherein the hydroxycitric acid is obtained form the aqueous extract of Garcinia pedunculate or Garcinia Cowa.
9. A process as claimed in claim 1, wherein the hydroxycitric content of encapsulated powder of extract is in the range of 53-62% on dry basis.
10. A process as claimed in claim 1, wherein the Whey protein content of the encapsulated powder of extract is in the range of 30 - 44 % determined by Bradfort method.
11. A process as claimed in claim 1, wherein encapsulated powder of Garcinia extract contains hydroxycitric acid in the native form.
12. A process as claimed in claim 1, wherein the encapsulated powder of Garcinia extract is a stable and non-hygroscopic.
13. A process as claimed in claim 1 wherein the encapsulated powder of Garcinia extract possess better bio-availability.
14. A process as claimed in claim 1, wherein the encapsulated powder of Garcinia extract provides essential amino acids.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2004569896A JP4408815B2 (en) | 2003-03-26 | 2003-03-26 | Process for encapsulation of Garcinia extract |
| AU2003226621A AU2003226621B2 (en) | 2003-03-26 | 2003-03-26 | A process for the encapsulation of garcinia extract |
| PCT/IN2003/000086 WO2004084654A1 (en) | 2003-03-26 | 2003-03-26 | A process for the encapsulation of garcinia extract |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/IN2003/000086 WO2004084654A1 (en) | 2003-03-26 | 2003-03-26 | A process for the encapsulation of garcinia extract |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO2004084654A1 true WO2004084654A1 (en) | 2004-10-07 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/IN2003/000086 WO2004084654A1 (en) | 2003-03-26 | 2003-03-26 | A process for the encapsulation of garcinia extract |
Country Status (3)
| Country | Link |
|---|---|
| JP (1) | JP4408815B2 (en) |
| AU (1) | AU2003226621B2 (en) |
| WO (1) | WO2004084654A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012012844A1 (en) * | 2010-07-29 | 2012-02-02 | The University Of Sydney | Vegetable and fruit juice powder |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110771903B (en) * | 2019-10-22 | 2022-05-17 | 浙江索契壹营养科技有限公司 | Whey protein powder and preparation method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5536516A (en) * | 1994-08-24 | 1996-07-16 | Renaissance Herbs, Inc. | Hydroxycitric acid concentrate and food products prepared therefrom |
| US5783603A (en) * | 1995-05-15 | 1998-07-21 | Sabinsa Corporation | Potassium hydroxycitrate for the suppression of appetite and induction of weight loss |
| US5911992A (en) * | 1997-06-12 | 1999-06-15 | A. Glenn Braswell | Method for controlling weight with hypericum perforatum and garcinia cambogia |
| US6160172A (en) * | 1997-08-27 | 2000-12-12 | Vittal Mallya Scientific Research Foundation | Soluble double metal salt of group IA and IIA of (-) hydroxycitric acid, process of preparing the same and its use in beverages and other food products without effecting their flavor and properties |
| US6447807B1 (en) * | 1999-09-14 | 2002-09-10 | Dallas L. Clouatre | Potassium (-)-hydroxycitric acid methods for pharmaceutical preparations for stable and controlled delivery |
-
2003
- 2003-03-26 WO PCT/IN2003/000086 patent/WO2004084654A1/en active Application Filing
- 2003-03-26 AU AU2003226621A patent/AU2003226621B2/en not_active Ceased
- 2003-03-26 JP JP2004569896A patent/JP4408815B2/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5536516A (en) * | 1994-08-24 | 1996-07-16 | Renaissance Herbs, Inc. | Hydroxycitric acid concentrate and food products prepared therefrom |
| US5783603A (en) * | 1995-05-15 | 1998-07-21 | Sabinsa Corporation | Potassium hydroxycitrate for the suppression of appetite and induction of weight loss |
| US5911992A (en) * | 1997-06-12 | 1999-06-15 | A. Glenn Braswell | Method for controlling weight with hypericum perforatum and garcinia cambogia |
| US6160172A (en) * | 1997-08-27 | 2000-12-12 | Vittal Mallya Scientific Research Foundation | Soluble double metal salt of group IA and IIA of (-) hydroxycitric acid, process of preparing the same and its use in beverages and other food products without effecting their flavor and properties |
| US6447807B1 (en) * | 1999-09-14 | 2002-09-10 | Dallas L. Clouatre | Potassium (-)-hydroxycitric acid methods for pharmaceutical preparations for stable and controlled delivery |
Non-Patent Citations (1)
| Title |
|---|
| GIBBS B F ET AL: "ENCAPSULATION IN THE FOOD INDUSTRY: A REVIEW", INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION, CARFAX PUBLISHING LTD, GB, vol. 50, no. 3, 1999, pages 213 - 224, XP009013399, ISSN: 0963-7486 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012012844A1 (en) * | 2010-07-29 | 2012-02-02 | The University Of Sydney | Vegetable and fruit juice powder |
| CN103108558A (en) * | 2010-07-29 | 2013-05-15 | 悉尼大学 | Vegetable and fruit juice powder |
Also Published As
| Publication number | Publication date |
|---|---|
| JP4408815B2 (en) | 2010-02-03 |
| AU2003226621A1 (en) | 2004-10-18 |
| JP2006521087A (en) | 2006-09-21 |
| AU2003226621B2 (en) | 2007-09-06 |
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