WO2004055511A1 - 非天然系有機化合物の生分解率の測定方法 - Google Patents
非天然系有機化合物の生分解率の測定方法 Download PDFInfo
- Publication number
- WO2004055511A1 WO2004055511A1 PCT/JP2003/015992 JP0315992W WO2004055511A1 WO 2004055511 A1 WO2004055511 A1 WO 2004055511A1 JP 0315992 W JP0315992 W JP 0315992W WO 2004055511 A1 WO2004055511 A1 WO 2004055511A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- biodegradation
- organic compound
- measuring
- medium
- biodegradation rate
- Prior art date
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/24—Earth materials
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
- Y10T436/109163—Inorganic standards or controls
Definitions
- the present invention relates to a method for measuring a biodegradation rate of a non-natural organic compound. More specifically, the present invention relates to a method for measuring the biodegradation rate of non-natural organic compounds using a change in the radioactive carbon isotope “C” from modern carbon, in a safe, clear, convenient, fast, and accurate manner.
- Background art
- the present invention provides a method for measuring the biodegradation rate of non-natural organic compounds by taking special radiation measures. It is an object of the present invention to provide a method that can perform safe, simple, quick and accurate measurement with little measurement error.
- the present inventors have conducted intensive studies in order to solve such a problem, and as a result, have found that the decaying radioactive carbon isotope 14 c has been completely destroyed and does not remain in the non-natural organic compound.
- the 14 C concentration in carbon dioxide from the medium mixed with the measurement sample when performing biodegradation, and measuring the difference between the “C concentration” and the “C concentration of modern carbon”, It has been found that the biodegradation rate of non-natural organic compounds can be measured.
- using the metal contained in the biodegradation medium as an internal standard enables the measurement of the biodegradation rate in an open field, which was not possible conventionally, and based on these findings.
- the present invention has been completed. That is, the gist of the present invention is as follows.
- a method for measuring the biodegradation rate of a non-natural organic compound which comprises measuring the biodegradation rate of a non-natural organic compound from the difference from the C concentration.
- FIG. 1 is an explanatory diagram of an example of a biodegradation reaction device used for carrying out the present invention.
- the method for measuring the biodegradation rate of a non-natural organic compound comprises measuring the concentration of a radiocarbon isotope "C" in the biodegradation medium in the biodegradation of the non-natural organic compound in the presence of the biodegradation medium. Measuring the biodegradation rate of the non-natural organic compound from the difference between the "C concentration and the 14 C concentration of the modern carbon. This is a method for measuring the biodegradation rate.
- the non-natural organic compounds used for measuring the biodegradation rate in the present invention include, for example, various petrochemical products and coal chemical products synthesized from fossil fuels such as petroleum, coal and natural gas. Of various organic compounds.
- substances such as synthetic detergents that have a large risk of adversely affecting the natural environment, such as draining into the natural world together with wastewater after use and contaminating the water quality of rivers and lakes, are particularly important. It is a substance for which it is necessary to examine the progress of biodegradation by microorganisms in nature.
- the carbon-containing materials of its natural system, Te carbon dioxide or plant odors in the atmosphere such as, but 1 mole of carbon is 6.
- the half-life of the radioactive carbon isotope 1 4 C is 5, since 7 is 3 0 ⁇ 4 0 years, these 6 X 1 0 1 1 single radiocarbon 1 4 C collapses all It will take 226,000 years to complete.
- 1 4 C concentration has a value which is calculated with 1 4 C concentration in the circulation carbon time 1 9 5 0 years 1 0 0% by international agreement, which p MC ( percent Modern Carbon). 1 9 5 0 onwards, the concentration of radioactive carbon isotope 1 4 C in the carbon dioxide in the atmosphere is varied, 1 4 C concentration in the carbon Contemporary (2 0 0 2 years) 1 1 0 It is within the range of 1 1 1%.
- the sample for measuring the biodegradability rate is a non-natural organic compounds such as petrochemical products that are synthesized fossil fuels as a raw material, radiocarbon 1 4 c
- a natural substance containing, which has a function of causing the biodegradation of non-natural organic compounds in the sample for example, compost, activated sludge, soil suspension, etc.
- a biodegradation reaction of the sample is performed at a predetermined temperature while introducing air from which carbon dioxide in the atmosphere has been completely removed into the vicinity of the bottom in the reaction tank 1.
- the air supplied to the reaction tank 1 is introduced into the reaction tank 1 via a carbon dioxide absorption tank 2 containing an aqueous sodium hydroxide solution and a washing tank 3 as shown in FIG. This prevents atmospheric carbon dioxide from entering the reaction system. Except for a part of the air introduced into the reaction vessel 1, except for a part consumed in the biodegradation reaction, together with the carbon dioxide generated by the biodegradation reaction, from the vicinity of the top of the reaction vessel 1, the storage tank 4, the first stage Introduced into trap 5, second stage trap 6. After the biodegradation reaction is carried out for a predetermined period of time, for example, 10 days, the medium containing the non-natural organic compound of the biodegraded sample is similarly removed from air containing carbon dioxide or pure oxygen.
- the content (p MC) of the radioactive carbon isotope 14 C in carbon dioxide can be measured using a scintillation counter or an accelerator mass spectrometer.
- the biodegradation rate of the sample to be measured is determined by adding pMC before and after biodegradation of the medium containing the sample obtained as described above and fertilizer without adding any non-natural organic compounds in the sample.
- a medium consisting only of natural products such as, for example, the value of the radioactive carbon isotope 14C content (pMC of the control medium) determined in the same manner, and using these values Then, it can be obtained by the following formula.
- the medium for performing the biodegradation contains metal species such as iron, copper, and manganese, and these metal species are not biodegraded even when the biodegradation operation is performed. Since no volatilization occurs, the total amount of these metal species hardly changes before and after the biodegradation operation. Therefore, using this property, the biodegradation rate can also be obtained by the following formula using the metal species in the biodegradation ground as an internal standard.
- a biodegradation test of polyproprolactone in compost was conducted using the apparatus shown in Fig. 1 as a biodegradation reactor.
- a biodegradation medium is prepared by mixing 5.0 g of polycaprolactone (hereinafter referred to as “PCL”) as a compound, and 4% by mass of the whole is sampled for analysis, and the rest is collected in a reaction vessel 1 Filled.
- PCL polycaprolactone
- reaction tank 1 was charged into a constant temperature bath at 58 ° C, and low-pressure air was removed through the carbon dioxide absorption tank 2 and the washing tank 3 to remove atmospheric carbon dioxide.
- the air was introduced from the air introduction pipe inserted near the bottom.
- An aqueous solution of sodium hydroxide having a 2N concentration was introduced into the carbon dioxide absorption tank 2 to absorb carbon dioxide in the atmosphere.
- the air flow rate was 30 milliliter / minute.
- excess air from the vicinity of the top of the reaction tank 1 and carbon dioxide generated by the biodegradation reaction in the reaction tank 1 are introduced into the storage tank 4 via a conduit, and the conduit from the outlet of the storage tank 4 is connected to the first conduit. It was introduced into the aqueous sodium hydroxide solution in the step trap 5.
- a second-stage trap 6 was provided downstream of the first-stage trap 5 to discharge only excess air to the atmosphere.
- PCL was biodegraded in the compost for one day.
- the biodegraded medium is burned in a combustion furnace using air from which carbon dioxide has been removed through the same carbon dioxide absorption tank as the biodegradation reactor described above, and the combustion gas is captured by a trap containing an aqueous sodium hydroxide solution. Then, the amount of carbon dioxide in the aqueous solution and its pMC value were determined.
- the biodegradation rate of a non-natural organic compound can be measured safely, simply, quickly, and accurately, and in the case of a method using an internal standard, it is further opened to the atmosphere. It is possible to provide a method for measuring the biodegradation rate of a non-natural organic compound, which also has the effect of being able to be measured with a configured device.
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Geology (AREA)
- General Life Sciences & Earth Sciences (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Environmental & Geological Engineering (AREA)
- Remote Sensing (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
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- Sampling And Sample Adjustment (AREA)
Abstract
Description
Claims
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US10/539,805 US7642094B2 (en) | 2002-12-18 | 2003-12-12 | Method of measuring biodegradation rate of unnatural organic compound |
EP03778911A EP1574852A4 (en) | 2002-12-18 | 2003-12-12 | METHOD FOR MEASURING THE BIODEGRADABLE SPEED OF A NON-NATURAL ORGANIC COMPOUND |
CA002510757A CA2510757A1 (en) | 2002-12-18 | 2003-12-12 | Method of measuring biodegradation rate of non-natural organic compound |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2002-366721 | 2002-12-18 | ||
JP2002366721A JP4233863B2 (ja) | 2002-12-18 | 2002-12-18 | 非天然系有機化合物の生分解率の測定方法 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2004055511A1 true WO2004055511A1 (ja) | 2004-07-01 |
Family
ID=32588321
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/JP2003/015992 WO2004055511A1 (ja) | 2002-12-18 | 2003-12-12 | 非天然系有機化合物の生分解率の測定方法 |
Country Status (5)
Country | Link |
---|---|
US (1) | US7642094B2 (ja) |
EP (1) | EP1574852A4 (ja) |
JP (1) | JP4233863B2 (ja) |
CA (1) | CA2510757A1 (ja) |
WO (1) | WO2004055511A1 (ja) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2007085874A (ja) | 2005-09-21 | 2007-04-05 | Idemitsu Kosan Co Ltd | 試料中の循環炭素含有量の測定方法及び試料中の循環炭素物質の含有率測定法 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH05180830A (ja) * | 1991-12-26 | 1993-07-23 | Showa Shell Sekiyu Kk | 油状物質の生分解性試験法 |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5284995A (en) * | 1993-03-08 | 1994-02-08 | The United States Of America As Represented By The Secretary Of The Army | Method to extract and recover nitramine oxidizers from solid propellants using liquid ammonia |
GB2314078B (en) * | 1996-06-14 | 2000-06-07 | British Gas Plc | Biodegradation of iron cyanide complexes |
-
2002
- 2002-12-18 JP JP2002366721A patent/JP4233863B2/ja not_active Expired - Fee Related
-
2003
- 2003-12-12 US US10/539,805 patent/US7642094B2/en not_active Expired - Fee Related
- 2003-12-12 WO PCT/JP2003/015992 patent/WO2004055511A1/ja active Application Filing
- 2003-12-12 EP EP03778911A patent/EP1574852A4/en not_active Withdrawn
- 2003-12-12 CA CA002510757A patent/CA2510757A1/en not_active Abandoned
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH05180830A (ja) * | 1991-12-26 | 1993-07-23 | Showa Shell Sekiyu Kk | 油状物質の生分解性試験法 |
Non-Patent Citations (2)
Title |
---|
LARSON R J: "Comparison of biodegradation rates in laboratory screening studies with rates in natural waters", RESIDUE REVIEWS, vol. 85, 1983, pages 159 - 171, XP002979604 * |
See also references of EP1574852A4 * |
Also Published As
Publication number | Publication date |
---|---|
JP2004198239A (ja) | 2004-07-15 |
US20060160228A1 (en) | 2006-07-20 |
JP4233863B2 (ja) | 2009-03-04 |
EP1574852A1 (en) | 2005-09-14 |
CA2510757A1 (en) | 2004-07-01 |
US7642094B2 (en) | 2010-01-05 |
EP1574852A4 (en) | 2008-08-06 |
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