METHOD FOR ENRICHING CO2 CONCENTRATIONS IN A PLANT ENVIRONMENT
INTRODUCTION This invention relates to a method of increasing CO2 concentrations in a plant environment. The invention also extends to a solution suitable for treating plants to increase absorption of the available CO2 concentrations.
BACKGROUND TO THE INVENTION It is generally appreciated that the process of photosynthesis is the primary mechanism of energy input into the living world. Photosynthesis, which occurs in all plants, basically involves oxidation of water and reduction of carbon dioxide (CO2) to produce organic compounds such as carbohydrates. It is these organic compounds that serve as a source of energy and as carbon skeletons for normal metabolism and production of biomass. The overall reaction can be summarized as follows:
6CO2 + 6H20 + sunlight ► C6Hι2 O6 + 6O2
It is estimated that the world primary production due to photosynthetic activity exceeds 170 gigatons (170 x 109 metric tonnes) of dry plant matter per annum. This translates to an annual fixation in excess of 70 billion metric tons of carbon per year.
According to the process of photosynthesis, plants absorb C02 from the atmosphere by diffusion through the plant stomata. Once inside the leaf, C02 dissolves in water vapor in the stomatal space and in a thin water layer surrounding plant cells to form bicarbonate, according to the following basic reaction:
H2O + CO2 ► HCO3 " + H+
The HCO3 " then diffuses into the plant cells and chloroplast where it is used in the production of photosynthates.
The amount of C02 in normal atmospheric air is generally in the order of approximately 400 μmol mol"1 (ppm). In most C3-species, this level is non-saturating due to an effect of photorespiration. In contrast, most C4-species are saturated at around 400 ppm.
It is generally appreciated by those engaged in the industry that photosynthetic rates are enhanced by higher concentrations of CO2 in the atmosphere, resulting in higher productivity and increased rates of photosynthesis. An increase in CO2 concentration causes a decrease in photorespiration, resulting in increased CO2 absorption by the plant. Accordingly, growers of greenhouse crops commonly enrich the atmosphere within a greenhouse with CO2 in order to increase yields. The CO2 levels in a greenhouse atmosphere can often be increased to as high as 1000 to 1200 ppm before resulting in stomatal closure and phytotoxicity. Enrichment is usually not done outside enclosed environments, because any air movement easily blows the CO2 gas away.
In recent years, mostly due to an increase in the concentration of "greenhouse" gasses, most notably CO2, there have been quite a few studies conducted on the effect of increased C02 concentration on photosynthesis, various ecological niches and the environment as a whole. A review paper by Bazzaz and Fajer (Plant life in a CO∑-rich world, Scientific American, January 1992, 18-24) confirms a general beneficial effect in the form of increased rates of photosynthesis and yield in many plant species when
gaseous atmospheric CO2 concentration is increased. However, one of the disadvantages reported by some researches with regard to an increase in atmospheric CO2 concentration is the apparent detrimental effects suffered by other plant species when present in a mixed or multi-species plant community and also possibly by some species of insects and even animals higher up in the food chain. It appears as if the main cause of the detrimental effects experienced by some plant species, under conditions where gaseous atmospheric C02 concentrations were increased, is the indiscriminate application of CO2 gas.
Another problem associated with present methods of atmospheric C02 enrichment is the high costs associated with generation, transport and application of gaseous C02. Yet a further practical difficulty is the containment of the CO2 gas within a confined space, a specific surface area, for example within a green house or a particular field of crops.
Another major problem with the present practice is that due to global warming, international conventions have imposed limits on the discharge of "greenhouse" gasses, including CO2. As a result the use of gaseous C02 is, at best, a temporary measure.
OBJECT OF THE INVENTION
It is accordingly an object of the present invention to provide a method of increasing C02 concentrations in a plant environment that will overcome or minimize some of the detrimental effects experienced by conventional methods and processes of increasing CO2 concentrations, or at least to provide a useful alternative to such methods.
It is a further object of the invention to provide a solution suitable for treating a plant environment so as to increase CO2 concentrations within the plant environment.
SUMMARY OF THE INVENTION According to the invention there is provided a method of increasing CO2 concentrations in a plant environment, the method providing for the application of a bicarbonate or carbonate solution to the plant environment, and more specifically applying the bicarbonate or carbonate solution to roots and/or foliage of plants.
The method may include treating the plant foliage with a spray, mist or fog of the bicarbonate or carbonate solution, and/or treating the plant roots by means of irrigation, hydroponics, aeroponics or the like soilless treatment means.
The solution may be prepared from a dilute bicarbonic and/or carbonic acid and/or from their various corresponding bicarbonate and/or carbonate salts. Also, it may be produced through the absorption and / or dissolution of gaseous carbon dioxide and / or carbon monoxide gases into water or an aqueous solution. In addition, the solution may contain small amounts of various organic acids, such as methanoic, ethanoic (acetic), propionic or similar acids and/or their corresponding salts.
The method further may include the step of activating the bicarbonate or carbonate solution before application by introducing the same into an electrolytic cell or an electrochemical activation unit. More specifically, the method provides for the pre- application activation of the bicarbonate or carbonate solution to enhance ready availability of CO2 to the plants in the form of bicarbonate ions, the method including the step of pre-treating the solution in a flow-through electrolytic module (FEM).
The concentration of salts in a feed stream to the electrolytic cell may be between 0.1 and 50g/l and preferably between about 2 and 3 g/l. The product stream from a 2 - 3 g/l feed stream may be diluted to a 20% or 1 in 5 solution, i.e. a concentration of 0.4 to 0.6 g/l in a final solution applied to the plants. It will however be appreciated that different plant species would require different concentrations.
The method according to the invention of increasing C02 concentrations in a plant environment may be applied inter alia to fresh produce, including fresh flowers such as chrysanthemums, during the production of seedlings and plant tissue culture, and during the production of a variety of crops such as lettuce and tomatoes.
Frequency of application may be dependent inter alia on plant identity, and weather, field and system conditions, but is typically done so as to ensure that at least the majority of plant leaves on each plant are wetted. Depending on the plant species and the prevailing weather, field and system conditions, the applicant has found good results with foliage spray applications in the order of 24 times per day, preferably during daylight hours and for durations of from about 1 second to several minutes at a time. Optimal results were achieved with applications of 1 minute at a time, and at about 4 to 8 times a day.
According to another aspect of the invention there is provided a bicarbonate or carbonate solution suitable for treating a plant environment so as to increase CO2 concentrations within the plant environment, the bicarbonate or carbonate solution being characterized therein that it is treated in an electrolytic cell or an electrochemical activation unit before being applied to the plant environment.
According to yet a further aspect of the invention there is provided the use of a bicarbonate or carbonate solution in the treatment of a plant environment so as to increase CO2 concentrations within the plant environment.
According to yet another aspect of the invention there is provided a method of treating plants so as to increase CO2 concentrations in the same, the method providing for the application of a bicarbonate or carbonate solution to the plant environment, and more specifically applying .the bicarbonate or carbonate solution to roots and/or foliage of the plants.
The applicant believes that this method of increasing C02 concentrations in a plant environment permits judicious choice in the simultaneous application of CO2 at various appropriate concentrations to different crops. Not only can concentration levels be controlled accurately, but also applications can be limited to designated target areas. Furthermore, C02 is applied in its bicarbonate form, which is the form in which CO2 is naturally absorbed by the plant. This facilitates uptake and distribution in the plant. In addition, the limitations and expenses of enrichment with CO2 gas can be overcome by applying CO2 in the form of HCO3 ".
SPECIFIC EMBODIMENT OF THE INVENTION
Without limiting the scope thereof, the invention will now further be illustrated and exemplified with reference to the following non-limiting examples.
Figure 1 illustration of stunted root growth in anoiyte treated, hydropoπically grown lettuce plants; root growth in the anoiyte water and anoiyte spray and water treated plants is clearly stunted, when compared with the control and anoiyte spray treated plants (from left to right: control, anoiyte spray, anoiyte spray and water, anoiyte water).
wth rate results:
Figure 2 Illustration of increased growth rate of hydropoπically grown lettuce treated with anoiyte; a clear gradient can be seen, with anoiyte water treated plants having the highest growth rate, followed by the anoiyte spray and water-, anoiyte spray treated, and control. From left to right: control, anoiyte spray, anoiyte spray
and water, anoiyte water.
Chlorophyll content was measured when tomatoes were harvested. The following table and graph shows the difference in chlorophyll content in the leaves of tomato plants.
TABLE ι CHLOROPHYLL CONTENT [SPAD]
CHLOROPHYLL CONTENT IN TOMATO PLANTS
TREATMENT
The following tables and graph show the firmness of tomatoes in various ripening stages and in all treatments.
The following tables and graph show vitamin c content in mg l in various ripening stages of tomatoes in all treatments.
Vtamin c content invarious ripening stages of tomatoes
The following tables and graph show the content of soluble sugars in various stages of tomatoes in ail treatment.
The following tables and graph show the rate of ripening of tomatoes at 23°C.
The following tables show an increase in the number of tomatoes during growth in ail treatments.
THE EFFECT OF ANOLYTE ON YIELD OF TOMATOES
week week week week week week week week week week week 5 6 7 8 9 10 11 12 13 14 15
D Control 3 Spray treatment D Spray & water treatment
THE EFFECT OF ANOLYTE ON GROWTH (STEM DIAMETER)
OF TOMATO PLANTS
week week week week week week week week week week 1 2 3 4 5 6 7 8 9 10 α Control FJ spray treatment □ spray & water treatment
The following tables and graphs show the Preharvest measurements that were made, namely: plant height (cm), stem diameter (cm) and number of tomatoes.
EFFECT OF ANOLYTE ON GROWTH (PLANT HEIGHT) OF
TOMATO PLANTS.
week week week week week week week week week week 1 2 3 4 5 6 7 8 9 10
! □ Contro 3 spray tratment D Spray & water treatment
Figure 3 Increased growth is visible in plant sprayed with anoiyte (B), when compared to the control (A).
Figure 4 Cross-section of vascular tissue in the leaf of anoiyte watered plants before flower induction.
Figure 5 Cross-section of vascular tissue in the leaf of anoiyte sprayed plants before flower induction.
Figure 6 Cross-section of vascular tissue in the stem of anoiyte watered plants before flower induction.
Figure 7 Cross-section of vascular tissue in the stem of anoiyte sprayed plants before flower induction.
Figure 8 Control (left), and EAW treated tomato plants (right) at ZZ2 in the Limpopo province
90 o o o
O
90 o Figure 9 Control (left) and EAW treated Macadamia nut seedlings (for two weeks), Limpopo Province o o
O
90 Figure 10. Control (foreground) and EAW treated lettuce in the background (two weeks old) , North of Pretoria
©
© ©
O
Figure 11. Control (left, top and bottom) and EAW treated (right, top and bottom) lettuce, Johannesburg
TRIAL RESULTS: RADICAL WATERS
C5 IΛ
© BLOCK 6 ©
© RW.0.66Ha KTRL. 1.4Ha
© ©
H Uα.
90
© ©