WO2004033673A8 - Production process for plant biomass and its use - Google Patents

Production process for plant biomass and its use

Info

Publication number
WO2004033673A8
WO2004033673A8 PCT/IT2002/000645 IT0200645W WO2004033673A8 WO 2004033673 A8 WO2004033673 A8 WO 2004033673A8 IT 0200645 W IT0200645 W IT 0200645W WO 2004033673 A8 WO2004033673 A8 WO 2004033673A8
Authority
WO
WIPO (PCT)
Prior art keywords
cellular
cell line
interest
clusters
liquid culture
Prior art date
Application number
PCT/IT2002/000645
Other languages
French (fr)
Other versions
WO2004033673A2 (en
WO2004033673A3 (en
Inventor
Toso Roberto Dal
Giovanna Pressi
Monte Renzo Dal
Original Assignee
I R B Istituto Di Ricerche Bio
Toso Roberto Dal
Giovanna Pressi
Monte Renzo Dal
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by I R B Istituto Di Ricerche Bio, Toso Roberto Dal, Giovanna Pressi, Monte Renzo Dal filed Critical I R B Istituto Di Ricerche Bio
Priority to AU2002349829A priority Critical patent/AU2002349829A1/en
Priority to PCT/IT2002/000645 priority patent/WO2004033673A2/en
Publication of WO2004033673A2 publication Critical patent/WO2004033673A2/en
Publication of WO2004033673A8 publication Critical patent/WO2004033673A8/en
Publication of WO2004033673A3 publication Critical patent/WO2004033673A3/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018Culture media for cell or tissue culture
    • C12N5/0025Culture media for plant cell or plant tissue culture
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/04Plant cells or tissues

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Botany (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to a production process for plant biomass and the subsequent use thereof in the production of substances of industrial interest, in particular biologically active substances. More in particular, the present invention relates to a method for the cultivation of a plant cell line capable of producing metabolites of interest, comprising a selection phase for the cell line which includes the following cycle of operations: a) cultivating on solid medium a predetermined stabilized plant cell line for a time sufficient to obtain the multiplication of said cell line to give substantially distinct cellular clusters; b) taking from said solid medium said substantially distinct cellular clusters and placing each of them into a distinct liquid culture medium; c) cultivating each of said substantially distinct cellular clusters in said liquid culture medium for a time sufficiently long to allow the multiplication of said cellular clusters and the analytical determination of the primary and/or secondary metabolites produced by it; d) operating a qualitative and quantitative determination of the primary and/or secondary metabolites produced by each of said cellular clusters in said liquid culture medium; e) selecting the cellular cluster capable of producing the greatest quantity of said metabolite of interest; f) repeating the cycle of operations according to steps a), b), c), d) and e) on said cellular cluster selected according to step e) for a sufficient number of times until the quantity of said metabolite of interest produced by a selected cellular cluster and by the cellular cluster derived from a successive cycle of the selection operation remains substantially unchanged.
PCT/IT2002/000645 2002-10-10 2002-10-10 Production process for plant biomass and its use WO2004033673A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2002349829A AU2002349829A1 (en) 2002-10-10 2002-10-10 Production process for plant biomass and its use
PCT/IT2002/000645 WO2004033673A2 (en) 2002-10-10 2002-10-10 Production process for plant biomass and its use

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/IT2002/000645 WO2004033673A2 (en) 2002-10-10 2002-10-10 Production process for plant biomass and its use

Publications (3)

Publication Number Publication Date
WO2004033673A2 WO2004033673A2 (en) 2004-04-22
WO2004033673A8 true WO2004033673A8 (en) 2005-02-24
WO2004033673A3 WO2004033673A3 (en) 2007-12-27

Family

ID=32088961

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IT2002/000645 WO2004033673A2 (en) 2002-10-10 2002-10-10 Production process for plant biomass and its use

Country Status (2)

Country Link
AU (1) AU2002349829A1 (en)
WO (1) WO2004033673A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ITMI20050498A1 (en) * 2005-03-24 2006-09-25 Giuliani Spa COMPOSITION BASED ON VEGETABLE EXTRACTS BY AJUGA REPTANS TO PREVENT HAIR DROP DOWN STIMULAR HAIR GROWTH ADJUST THE PRODUCTION OF SEBUS
EP1997501B1 (en) 2005-06-20 2015-07-22 I.R.B. Istituto Di Ricerche Biotecnologiche S.r.l. Isoteupolioside and uses
EP1736167B1 (en) * 2005-06-20 2018-03-21 I.R.B. Istituto Di Ricerche Biotecnologiche S.r.l. Extracts obtained from cell line cultures from Syringa vulgaris IRB-SV25/B (DMS:16857), their preparation and use
US11299700B1 (en) 2021-02-19 2022-04-12 Acequia Biotechnology, Llc Bioreactor containers and methods of growing hairy roots using the same

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3950227A (en) * 1970-12-21 1976-04-13 St. John's University Batch method of establishing and maintaining a controlled aerobic environment for a microbial culture
JPS5716692A (en) * 1980-07-01 1982-01-28 Nippon Paint Co Ltd Cultivating method of plant cell
DE3247610A1 (en) * 1982-12-23 1984-07-05 A. Nattermann & Cie GmbH, 5000 Köln Process for the preparation of rosmarinic acid from plant cell cultures and of plant cell pellets containing rosmarinic acid
DE3584317D1 (en) * 1984-06-14 1991-11-14 Teijin Ltd METHOD FOR BREEDING ANIMAL OR VEGETABLE CELLS.

Also Published As

Publication number Publication date
AU2002349829A1 (en) 2004-05-04
WO2004033673A2 (en) 2004-04-22
AU2002349829A8 (en) 2004-05-04
WO2004033673A3 (en) 2007-12-27

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