WO2004027068A2 - Systemes d'expression de proteines toxiques, vecteurs et procede de fabrication de proteines toxiques - Google Patents
Systemes d'expression de proteines toxiques, vecteurs et procede de fabrication de proteines toxiques Download PDFInfo
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- WO2004027068A2 WO2004027068A2 PCT/FR2003/002763 FR0302763W WO2004027068A2 WO 2004027068 A2 WO2004027068 A2 WO 2004027068A2 FR 0302763 W FR0302763 W FR 0302763W WO 2004027068 A2 WO2004027068 A2 WO 2004027068A2
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/24011—Flaviviridae
- C12N2770/24211—Hepacivirus, e.g. hepatitis C virus, hepatitis G virus
- C12N2770/24222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
Definitions
- the present invention relates to toxic protein expression systems, expression vectors comprising one of these systems, prokaryotic cells transformed by these systems, as well as to a method of synthesis of a toxic protein using these expression systems.
- E. coli Escherichia coli
- proteins or peptides hydrophobic toxic for example the overproduction of transmembrane domains of proteins of envelope of virus.
- the determination of the three-dimensional (3D) structure is a decisive step in the structural and functional understanding of proteins. Very great efforts and means have been, and are, being implemented to achieve this goal, and have been amplified with the accumulation of data provided by genome sequencing programs [1].
- the two main techniques for establishing these protein structures are X-ray diffraction, performed from crystallized proteins, and nuclear magnetic resonance (NMR) performed from proteins in solution. NMR, well suited to the study of proteins with a molecular mass of less than 20 kDa, requires, like X-ray diffraction, the production of large quantities of material. In most cases, it also involves preparing material enriched with 15 N and / or 13 C.
- the bacterium is a means of production widely used by the scientific community [2].
- the overexpression of proteins in the bacteria is not without problems, however. Indeed, it gives rise to three scenarios:
- the first case is where the protein is overproduced in a form correctly folded in space during its synthesis in vivo. It is not a rare situation, but it is not frequent either. It essentially concerns soluble proteins of reduced size, that is to say about 20 to 50 kDa.
- the second and more common case is where the protein is overproduced and aggregated as an inclusion body.
- protein folding kinetics is significantly slower than its rate of biosynthesis. This promotes exposure to the aqueous solvent of the hydrophobic regions of the protein which are normally buried inside it, and generates non-specific interactions which lead to the formation of insoluble aggregates.
- the inclusion bodies can be solubilized / unfolded under non-native conditions, with urea or guanidine.
- the solubilized protein is then subjected to various treatments such as dialysis or dilution to promote, in certain cases with success, a native 3D folding.
- the third case is where the expression generates toxicity of varying degrees. This ranges from the absence of an expression product if the bacteria manages to adapt, to the death of the latter if the product is too toxic. This is a case which occurs quite frequently and most often with proteins or domains of membrane proteins, such as for example those of the envelope proteins of the hepatitis C virus [5] or of the human immunodeficiency virus. [6].
- the toxicity problem essentially concerns the expression of membrane proteins, that is to say proteins having a hydrophobic domain. These proteins are of growing interest. On the one hand, they are relatively numerous since the establishment of the various genomes confirms that they represent approximately 30% of the proteins potentially encoded by these genomes [7]. On the other hand, they constitute 70% of therapeutic targets and their alteration is at the origin of many genetic diseases [8].
- HCV hepatitis C virus
- This virus consists of a positive RNA of approximately 9500 bases which codes for a polyprotein of 3033 residues [13], symbolized in FIG. 1 appended by the rectangle 1A.
- This polyprotein is cleaved after expression by endogenous and exogenous proteases to give rise to 10 different proteins.
- El and E2 Two of them, are glycosylated and form the envelope of the virus. They each have membrane domains called TM, in particular TME1 for the protein E1 and TME2 for the protein E2.
- cleavage positions that generate them are indicated in Figure 1 by arrows with, mentioned below, a number which corresponds to the position in the polyprotein of the first amino acid of sequence resulting from cleavage.
- the proteins E1 and E2 are symbolized by a rectangle.
- the white part of each rectangle corresponds to 1 ectodomain (ed) and the gray part to the transmembrane region (TM).
- the primary sequence of TMs is indicated at the bottom of the figure in letter code, with numbers corresponding to the position of amino acids in the polyprotein located at the ends of these domains.
- the stars indicate hydrophobic amino acids.
- the object of the present invention is precisely to provide a system for expressing a toxic protein, which meets, inter alia, the needs indicated above.
- an expression system characterized in that it successively comprises, in the 5 ′ -3 ′ direction, a nucleotide sequence coding for the dipeptide Asp -Pro, called dp sequence below, and a nucleotide sequence (pt) coding for a toxic protein (Pt).
- dp sequence a nucleotide sequence coding for the dipeptide Asp -Pro
- pt a nucleotide sequence coding for a toxic protein (Pt).
- Pt toxic protein
- the expression system comprises in in addition, upstream of the dp sequence, a nucleotide sequence (ps) coding for a soluble protein (Ps).
- This soluble protein can be for example glutathione S-transferase (GST) or thioredoxin (TrX) or another equivalent soluble protein.
- GST glutathione S-transferase
- TrX thioredoxin
- This expression system according to the invention will be identified below by: ps-dp-pt.
- the dp-pt expression system of the present invention which comprises a sequence coding for Asp-Pro (DP in letter code) placed upstream of the nucleotide sequence of the toxic protein, makes it possible, quite unexpectedly, to suppress the toxic effect of the protein for the host cell.
- the inventors have noted that, quite surprisingly, the suppression of protein toxicity in the host is even more effective with the ps-dp-pt expression system, when the toxic peptide is produced in C-terminal fusion with a soluble protein, for example glutathione S-transferase or thioredoxin, with the Asp-Pro sequence inserted between the soluble protein and the toxic peptide.
- the dp-pt or ps-dp-pt expression system of the present invention allows the overproduction of toxic proteins in host cells, in particular hydrophobic proteins, in particular peptides which correspond to, or which comprise, hydrophobic domains of proteins anchored to membranes. It may for example be a membrane protein or a domain of a membrane protein. It may for example be a protein of a virus, for example a hepatitis C virus, an AIDS virus, or any another virus pathogenic for humans and generally for mammals.
- the dp-pt or ps-dp-pt system of the invention makes it possible to overproduce in a host such as E. coli the transmembrane domains of the proteins E1 and E2 of the hepatitis C virus, called TME1 and TME2, corresponding respectively to the sequences:
- TME2 717-MEYVVLLFLLLADARVCSCLWMMLLISQAEA-746 Sequence ID # 2
- the nucleotide sequences which can be used to constitute the dp-pt system of the invention coding for the proteins TMEl (dp-pt (TMEi)) or TME2 (dp-pt ( T E 2 )) can be all the possible sequences coding for the DP-TME1 and DP-TME2 fusion proteins.
- the sequences coding for the proteins TME1 and TME2 can be, for example, advantageously, respectively, sequences IDn ° 3 and IDn ° 4 from the annexed sequence list.
- the dp sequence coding for the dipeptide Asp-Pro (DP) is added to these sequences.
- nucleotide sequences which can be used to constitute the ps -dp-pt system of the invention coding for the TMEl (ps-dp-pt (T MEi)) or TME2 (ps-dp-pt ( T ME 2 )) proteins can be all possible sequences coding respectively for the Ps-DP-TME1 and Ps-DP-TME2 fusion proteins. It can be, for example, advantageously, sequences ID No. 34, ID No. 35 and ID No. 36 from the sequence list annexed for TME1, making it possible to obtain a chimeric protein Ps-DP-TME1. It can be, for example, advantageously, sequences ID No. 37, ID No. 38 and ID No.
- HCV RNA sequences producing an infectious phenotype there are a large number of HCV RNA sequences producing an infectious phenotype: these sequences can also be used in the present invention.
- the sequence coding for the Asp-Pro dipeptide can be for example: gacccg, or any other sequence coding for this dipeptide.
- the sequence coding for GST can be, for example, that present in plasmids pGEXKT, the sequence of which corresponds to sequence ID No. 29 of the annexed sequence list or any equivalent sequence, ie coding for this soluble protein.
- the sequence coding for TrX can be, for example, that present in the expression plasmid pET32a +, the sequence of which corresponds to sequence ID No. 30 of the annexed sequence list or any equivalent sequence, ie coding for this soluble protein. .
- the dp-pt or ps-dp-pt expression system of the invention is placed inside a host cell, for example by cloning in an appropriate plasmid and this by usual host transformation techniques in genetic recombination techniques.
- the plasmid in which the expression system of the present invention can be cloned to form this vector will be chosen in particular according to the host cell. It may be, for example, the plasmid pT7-7 (sequence ID No. 33 of the annexed sequence list), a plasmid of the pGEX series (for example sequence ID No. 31 of the annexed sequence list), marketed for example by the company Pharmacia, or a plasmid of the pET32 series (for example of sequence ID No. 32 from the annexed sequence list), marketed for example by the company Novagen.
- the plasmids of the pGEX series and of the pET32 series will be advantageously used for the implementation of the present invention. In fact, they already include a ps sequence coding for a soluble protein (Ps), respectively for glutathione S-transferase and for thioredoxin. Also, advantageously, the dp-pt system will be cloned into these plasmids downstream of this ps sequence coding for the soluble protein.
- Ps soluble protein
- the present invention therefore also relates to an expression vector comprising an expression system according to the invention dp-pt or ps-dp-pt; in particular a vector comprising a dp-pt expression system according to the invention and the oligonucleotide sequence of the plasmid pT7-7, or a vector comprising a ps-dp-pt expression system according to the invention and the oligonucleotide sequence d 'a plasmid pGEX or a plasmid pET32.
- the expression vectors of the present invention suitable for a bacterial host such as E.
- coli and allowing the overexpression of the above-mentioned membrane protein TME1, can advantageously have an oligonucleotide sequence chosen from sequences ID No. 40 (with pGEXKT), ID No. 42 (with pET32a +) and ID No. 44 (with pT7-7) from the attached sequence list.
- the expression vectors of the present invention suitable for a bacterial host such as E. coli, and allowing the overexpression of the above-mentioned membrane protein TME2, can advantageously have an oligonucleotide sequence chosen from sequences ID No. 41 (with pGEXKT), ID No. 43 (with pET32a +) and ID No. 45 (with pT7-7) from the attached sequence list.
- the aforementioned expression vectors have codons optimized for the expression of the chimeric proteins of the present invention, including TMEl and TME2, in a bacterium, for example in E. coli.
- the present invention also relates to a prokaryotic cell transformed with an expression vector according to the invention.
- This prokaryotic cell transformed by the expression vector of the present invention should preferably allow the overexpression of the toxic protein for which the vector codes.
- any host cell capable of expressing the expression vector of the present invention can be used, for example E. coli, advantageously, the E. coli BL21 (DE3) pLysS strain.
- the present invention also relates to a process for the manufacture of a toxic protein by genetic recombination comprising the following steps: - transforming a host cell with an expression vector according to the invention, cultivating the transformed host cell under culture conditions such that it makes a fusion protein comprising the dipeptide Asp-Pro followed by the peptide sequence of the toxic protein from said expression vector, and isolate said fusion protein, and cleave said fusion protein to recover the toxic protein .
- the stages of transformation, of culture, as well as of the isolation of the manufactured chimeric protein can be carried out by the usual techniques of genetic recombination, for example by techniques such as those which are described in the document [25].
- the step of isolating the fusion protein can be carried out by the usual techniques known to those skilled in the art for isolating a protein from a cell extract.
- the fusion protein made by the process of the invention has a sequence "Soluble protein-Asp-Pro-toxic protein".
- the Asp-Pro dipeptide is also called DP according to the letter code of amino acids.
- the toxic protein is
- the fusion protein may have the sequence ID No. 46 of the annexed sequence list, which corresponds to the fusion protein GST-DP-TME1; sequence ID No. 48 from the attached sequence list, which corresponds to the fusion protein TrX-DP-TME1; or the sequence IDn 0 50 from the annexed sequence list, which corresponds to the fusion protein M-DP-TME1 from the annexed sequence list.
- the fusion protein can have the sequence ID No. 47 from the attached sequence list, which corresponds to the fusion protein GST-DP-TME2; sequence ID No. 49 from the attached sequence list, which corresponds to the fusion protein TrX-DP-TME2; or sequence ID No. 51 from the attached sequence list, which corresponds to the fusion protein M-DP-TME2 from the attached sequence list.
- the step of cleavage of this fusion protein can advantageously be carried out by means of formic acid which cleaves the fusion protein at the level of the Asp-Pro dipeptide. It can also be carried out by any suitable technique known to a person skilled in the art for recovering a protein from a sample from a fusion protein.
- the inventors are the first to have found a truly efficient system for producing and even overproducing, in particular in the bacterium Escherichia coli (E. coli) hydrophobic peptides corresponding to the membrane domains of the proteins El and E2 of the envelope of the virus. hepatitis C, the expression of which is lethal to the microorganism.
- the field of applications of the present invention relates mainly to the production of hydrophobic peptides on a large scale, in particular for fundamental and industrial research.
- the production of the chimeric protein consisting of the soluble protein, the Asp-Pro dipeptide, and the hydrophobic peptide can be used for a functional purpose, in particular to provide information on the degree of oligomerization of the membrane domain or even on its capacity. heteropolymerization.
- Fusion proteins are produced via their coding DNA present for example in commercial plasmids and following which is introduced in phase the DNA coding for the Asp-Pro sequence followed by that coding for the toxic peptide.
- This application can be marketed in the form of bacterial expression plasmids which will include the sequence of the Asp-Pro site, downstream from that of the soluble proteins already present.
- the corresponding plasmid will be described for example as a tool facilitating the biological production of peptides or toxic membrane proteins.
- the present invention is applicable to any system of overexpression of recombinant proteins, with or without fusion to a soluble protein such as for example GST, or thioredoxin, including an unnatural Asp-Pro sequence inserted upstream of a coding sequence for a toxic domain of the protein, for example a membrane domain of a protein.
- a soluble protein such as for example GST, or thioredoxin
- an unnatural Asp-Pro sequence inserted upstream of a coding sequence for a toxic domain of the protein, for example a membrane domain of a protein.
- Sequences IDn ° 1 and 2 respectively, peptide sequences of TMEl and TME2.
- Sequences IDn ° 3 and 4 respectively, sequences coding for the peptide TMEl and the peptide TME2.
- Sequences ID No. 5 and 6 respectively, oligonucleotide (+) of insertion into pT7-7 (OL13 (+)) and oligonucleotide (-) of insertion into pT7-7 (OL14 (-)).
- Sequences IDn ° 7 and 8 respectively, sense DNA coding for TMEl + site cla I in 3 'and sense anticoding DNA for TMEl + site cla I in 5' (sequence complementary to sequence IDn ° 7).
- - Sequences IDn ° 9 and 10 respectively, oligonucleotide sense coding (0L11 (+)) and oligonucleotide sense anticoding (OL12 (-)) for the synthesis of TMEl.
- Sequence ID no. 11 oligonucleotide (+) of insertion into pGEXKT without dp site (0L15 (+)).
- Sequence ID No. 12 insertion oligonucleotide (+) in pGEXKT with dp site (OL17 (+)).
- Sequence ID No. 13 insertion oligonucleotide (-) in pGEXKT (OL16 (-)).
- - Sequence ID No. 14 insertion oligonucleotide (+) in pET32a (OL18 (+)) (hybridizes to segment 915-932 of pGEXKT).
- Sequences IDn 0 15 and 16 respectively, oligonucleotides (+) (OL19 (+)) and (-) (OL20 (-)) of insertion in pT7-7 of the DNA coding for MDP-TME1.
- sequences 17 and 18 respectively, insertion oligonucleotide (+) in pT7-7 (OL23 (+)) and insertion oligonucleotide (-) in pT7-7 (OL24 (-)).
- Sequences ID No. 19 and 20 respectively, sense DNA coding for TME2 + Nde I site in 5 ′ and Hind III in 3 ′; and DNA sense anticoding of TME2 + site Nde I in 3 'and Hind III in 5' (complementary sequence of IDn ° 17).
- Sequences IDn ° 21 and 22 respectively, sense oligonucleotides coding (OL21 (+)) and anticoding (OL22 (-)) for the synthesis of TME2.
- sequences ID No. 24 and 25 respectively, oligonucleotides (+) (OL27 (+)) and (-) (OL26 (-)) of insertion into pGEXKT with dp site.
- Sequences IDn 0 26 and 27 respectively, oligonucleotides (+) (OL28 (+)) and (-) (OL29 (-)) of insertion into pT7-7 of the DNA coding for MDP-TME2.
- Sequence ID No. 28 end of the sequence of the soluble protein GST followed by the thrombin site encoded in the plasmid pGEXKT.
- Sequence ID No. 29 DNA coding for the GST protein in the plasmid pGEXKT.
- Sequence IDn 0 30 DNA coding for thioredoxin (TrX) in the plasmid pET32a- Sequences IDn ° 31, 32 and 33: respectively, expression plasmids pGEXKT, pET32a + and pT7-7.
- - Sequences ID No. 34, 35 and 36 respectively, expression systems according to the invention coding for the fusion proteins GST-DP-TME1, TrX-DP-TME1 and M-DP-TME1.
- Sequences ID No. 37, 38 and 39 respectively, expression systems according to the invention coding for the fusion proteins GST-DP-TME2, TrX-DP-TME2 and M-DP-TME2.
- Sequences ID No. 40 and 41 respectively, expression vectors pGEXKT-dp-pt T MEi and pGEXKT- dp-pt TM E 2 according to the invention coding for the fusion proteins GST-DP-TME1 and GST-DP-TME2 .
- Sequences ID No. 42 and 43 respectively, expression vectors pET32a-dp-pt T MEi and pET32a-dp-pt T E 2 according to the invention coding for the fusion proteins TrX-DP-TME1 and TrX-DP-TME2 (code by the complementary strand).
- Sequences IDn 0 44 and 45 respectively, expression vectors pT7-7-dp-pt T MEi and pT7-7-dp-pt T ME 2 according to the invention coding for the fusion proteins M-DP-TME1 and M -DP-TME2.
- Sequences IDn 0 46 and 47 respectively, GST-DP-TME1 and GST-DP-TME2 fusion proteins according to the invention obtained from the plasmids pGEXKT- dp-ptMEi and pGEXKT-dp-pt T ME 2 .
- - Sequences IDn ° 48 and 49 respectively, TrX-DP-TMEl and TrX-DP-TME2 fusion proteins according to the invention obtained from the plasmids pET32a-dp-pt TME ⁇ and pET32a-dp-pt TME 2.
- Sequences ID No. 50 and 51 respectively, fusion proteins M-DP-TME1 and M-DP-TME2 according to the invention obtained from the plasmids pT7-7-dp-pt T MEi and pT7-7-dp-pt T ME 2 .
- Sequences IDn 0 52 and 53 respectively, GST and TrX proteins coded by the vector pGEXKT and pET32a +.
- Figure 1 schematic representation of part of the HCV polyproptein and peptide sequence of the C-terminal membrane domains of the envelope proteins E1 and E2.
- the peptide sequences shown correspond to the infectious type # D00831 and # M67463 for TME1 and TME2 respectively obtained from the public sequence bank of the European Molecular Biology Laboratory (EMBL).
- - Figure 2 creation of DNA coding for the C-terminal membrane domain of the HC1 envelope protein El and additional sequences in 5 'and 3' for cloning in different plasmids. The sequences shown in this figure are given in the attached sequence list.
- Figure 3 Creation of DNA coding for the C-terminal membrane domain of the HCV E2 envelope protein and additional sequences in 5 ′ and 3 ′ for cloning in different plasmids. The sequences shown in this figure are given in the attached sequence list.
- Panel 4 panels A to F: toxicity of the membrane domains expressed in the bacterium and suppression of this toxicity by insertion of a dp site.
- Panels A, C and E are graphical representations of measurements of the optical density (OD) at 600 nm as a function of time (t) in hours of the production of different proteins in a bacterium using or not the expression system of the present invention.
- Panels B, D and F are representations of the protein migration gels of panels A, C and E respectively.
- FIG. 5A is a graphic representation of measurements of the optical density (OD) at 600 nm as a function of the time in hours of the production of different proteins in a bacterium using or not using the expression system of the present invention.
- Figure 5B is a representation of a protein migration gel of Figure 5A.
- FIG. 6 expression and purification of the fusion protein (or chimera) GST-TME2, and comparison with GST alone. This figure shows at the top the peptide sequences of GST and GST-TME2, and below the gels obtained by electrophoresis showing that, unlike GST alone, GST-TME2 is insoluble. The latter is produced in the form of inclusion bodies which cannot fold properly.
- FIG. 7A and 7B graphical representations of comparative experimental results showing the effect of DP dipeptide (oligonucleotide sequence dp-pt in accordance with the present invention) and DP dipeptide and soluble protein (oligonucleotide sequence ps-dp-pt in accordance with the present invention) on the synthesis of toxic proteins TME1 and TME2 in accordance with present invention.
- the plasmid pET32a + was obtained from Novagen (http://www.novagen.com); the plasmids pT7-7 and pGPl-2 and the strain K38 [22] were requested from Prof. Tabor (Department of Biological Chemistry, Harvard Medical School); the plasmid pGEX-KT was requested from Prof. Dixon (Department of Biological Chemistry, University of Michigan Medical School); the other products were obtained from Sigma (http://sigma.aldrich.com).
- TME1 and TME2 peptides were first carried out without the expression system of the present invention, then in fusion with a soluble protein, and finally in fusion with GST with insertion of the Asp site - Pro (“DP” in letter coding) between the soluble protein and TMEl or TME2.
- SEQ IDn 0 is used for "sequence IDn °" and refers to the attached sequence list.
- the DNA encoding the two domains was synthesized de novo from the appropriate oligonucleotides.
- the codons were chosen according to their greatest frequency of use in the bacterium, as quantified by Sharp et al. [17].
- the constructions are described in FIG. 2 annexed for TME1 and in FIG. 3 annexed for TME2.
- Each synthetic DNA was generated using a set of two long oligonucleotides and overlapping, OLll (SEQ IDn ° 9) and OL12 (SEQ n ° 10) for TMEl and OL2KSEQ IDn ° 19) and OL22 (SEQ IDn ° 20) for TME2, which were amplified after hybridization with two external oligonucleotides chosen according to the cloning into a given plasmid.
- telomere sequenced in pT7-7 was carried out using the set of external oligonucleotides OL13 (SEQ IDn ° 5) and OL14 (SEQ IDn ° 6) for TMEl and OL23 (SEQ IDn ° 15) and OL24 (SEQ IDn ° 16 ) for TME2.
- Each synthetic DNA was generated using a set of four oligonucleotides: two long and overlapping and two short and external.
- the DNAs were amplified by the “polymerase chain reaction” method called “PCR” [18] and then cloned into a bacterial plasmid pCR (trademark) T7 topo TA.
- the synthesized DNAs were sequenced and then subcloned into the bacterial expression vector pT7-7 [19] using the 5 'Nde I restriction sites and 3' Cla I or Hind III restriction sites.
- C and D DNA matrix-less amplification strategy.
- the coding direction and the anticoding direction of the oligonucleotides are indicated respectively by the (+) and (-) signs.
- the long oligonucleotides overlap by around twenty bases to create the primer and then the template.
- the short oligonucleotides make it possible to amplify the matrix by PCR by integrating the desired restriction sites and according to the plasmids used.
- the insertion into pT7-7 was carried out with the pair of oligonucleotides OL13 (SEQ IDn ° 5) and OL14 (SEQ IDn ° 6), via a subcloning in pCRT7 topo, by integrating the Nde I and Hind III sites .
- the insertion into pGEXKT was carried out according to the same method with the pair of oligonucleotides OL15 (SEQ ID No. 11) and OL16 (SEQ ID No. 13) by integrating the BamR I and EcoR I sites.
- the insertion of the dp site ( gacccg) and cloning in pGEXKT were carried out with the oligonucleotide pair 0L17 (SEQ ID No. 12) and OL16 (SEQ ID No. 13).
- the construction in pGEXKT was transferred to pET32a which codes for thioredoxin with the pair of 1 oligonucleotides OL18 (SEQ ID No. 14) and 0L16 (SEQ ID No.
- the oligonucleotide OL18 hybridizes in the terminal region of the DNA coding for GST in pGEXKT.
- the amplified sequence integrates the end of the GST (SDLSGGGGG) followed by the thrombin site
- LVPRGS (SEQ ID No. 28), the DP site and the membrane passage.
- the DNA inserted into pET32a makes it possible to express the thioredoxin-SDLSGGGGGLVPRGS-DP-TME1 chimera (SEQ ID No. 48).
- pT7-7 The insertion in pT7-7 was carried out with the couple d 1 oligonucleotides OL23 and OL24 (respectively SEQ IDn 0 17 and SEQ IDn ° 18) via a subcloning in pCRT7 topo by integrating the Nde I and Hind III sites.
- the insertion into pGEXKT was carried out according to the same method with the pair of oligonucleotides OL25 and OL26 (respectively SEQ ID No. 23 and SEQ ID No. 25) integrating the BamH I and EcoR I sites. Insertion of the dp site (gacccg ) and the cloning in pGEXKT were carried out with the oligonucleotide pair OL27 and OL26 (SEQ ID No. 24 and SEQ ID No. 25 respectively). The insertion into pET32a was carried out as described in FIG. 2 using the pair of oligonucleotides OL18 and OL26 (respectively SEQ ID No. 14 and SEQ ID No. 25).
- the expression vectors pGEXKT-pt TM Ei and pGEXKT-pt TME2 were constructed by PCR as described in Figures 2 and 3 attached.
- the DNA template used to amplify the DNA coding for TME1 or TME2 is that cloned in the plasmids pT7-7.
- the cloning into the plasmid pGEXKT [20, 21] of TMEl was carried out using the sets of oligonucleotides OL15 (SEQ IDno ll) and OL16 (SEQ IDn 0 13) allowing the insertion of the BamE I restriction sites in 5 'and EcoR I in 3'.
- the cloning of TME2 in the same vector was carried out using the oligonucleotide sets OL25 (SEQ ID No. 21) and OL26 (SEQ ID No. 23).
- the insertion of the dp site in the N-terminal position of TME1 was carried out by replacing the oligonucleotide 5 ′ 0L15 (SEQ IDno # 11) with the oligonucleotide OL17 (SEQ IDno # 12).
- the insertion of the dp site in the N-terminal position of TME2 was carried out by replacing the oligonucleotide 5 'OL25 (SEQ ID No. 21) with the oligonucleotide OL27 (SEQ ID No. 22), as shown in FIG. 3.
- the expression vectors pET32a-dp-TME1 and pET32a-dp-TME2 were constructed by PCR as described in FIGS. 2 and 3 appended, using the set of oligonucleotides indicated.
- the upstream oligonucleotide integrates an EcoR V site and hybridizes with the terminal region of the gene coding for GST. It makes it possible to integrate the tail of 5 glycines and the cleavage site by thrombin present in the plasmid.
- the downstream oligonucleotide is the same as that used for cloning in pGEXKT.
- the insertion into the plasmid pET32a is done by the 5 'MsC I / EcoR V and 3' EcoR I sites. It makes it possible to insert in phase at the end of the thioredoxin sequence the tail of 5 glycines, the cleavage site by thrombin, the DP site and the membrane passage.
- the original plasmid pET32a which serves as a control, codes for thioredoxin followed by a sequence integrating various elements which have not been deleted and which contribute for a large part to the mass of the chimeric protein produced.
- the template DNA used to amplify the DNAs coding for TME1 or TME2 is that cloned in the plasmids pGEXKT-dp-pt T MEi or pGEXKT-dp-p T E2 •
- the cloning in pET32a + was carried out using the sets of oligonucleotides OL18 (SEQ IDn ° 14) and OL16 (SEQ IDn 0 13).
- the cloning of TME2 in the same vector was carried out using the sets of oligonucleotides OL18 (SEQ ID No. 14) and OL26 (SEQ ID No. 23), as indicated in FIG. 3.
- the plasmid pT7-7 contains the DNA to be expressed, placed under the control of a promoter ⁇ 10 recognized by the polyerase RNA of phage T7.
- the plasmid pGPl-2 contains the gene coding for the phage T7 polymerase, placed under the control of a promoter ⁇ p L. This promoter is repressed by a heat-sensitive repressor, cI857, also present in pGP1-2. At 30 ° C, cI857 is normally expressed and represses the promoter ⁇ pi ,, which blocks the expression of the polymerase and therefore also that of the protein of interest.
- Induction is triggered by passing the culture from 37 to 42 ° C for 15-30 min, then expression continues at 37 ° C.
- This system is therefore particularly well suited when it is necessary to strictly control the expression of a given protein, especially if it is toxic to the bacteria.
- the presence of the plasmid pLysS allows continuous expression and at a low level of lysozyme of phage T7. This inhibits the phage T7 polymerase, whose low expression in the absence of induction could allow the basal expression of toxic protein.
- the inventors have also tested the expression of the membrane domains alone in strains called C41 and C43 [10] which have been selected to resist the expression of toxic membrane proteins. These strains are derived from the BL21 (DE3) strain and are used in the same way as the latter.
- the corresponding plasmids were introduced by transformation into the different strains of E. coli: K38 (HfrC ⁇ ) for the Tabor thermal induction system or the different BL21 strains for chemical induction. Table 1 below summarizes the tests carried out.
- ten transformants were cultured to test the expression. Briefly, the bacteria were cultured in 5 ml of LB (10 g tryptone, 5 g yeast extract, 5 g NaCl, qs 1 liter H 2 0) supplemented with 50 ⁇ g / ml of ampicillin (necessary to maintain pT7 -7 in the bacteria) and 60 ⁇ g / ml of kanamycin (necessary for maintain pGPl-2 in the bacteria) and then cultured until saturation either at 30 ° C for K38, or at 37 ° C for BL21 (DE3). The cultures are then diluted 1/10 in the same culture medium and cultured to an optical density ' (OD) of 1 measured at 600 nm on a Philips PU8740 spectrophotometer (trademark).
- OD optical density '
- the expression is then induced either thermally (K38) at 42 ° C for 15 min, or chemically (BL21 (DE3)) by addition of 1 ri of IPTG. It is continued for 3-5 hours at 37 ° C. The DO ⁇ oonm of cultures is measured at different times.
- a volume of culture containing the equivalent of 0.1 OD of bacteria is taken.
- the bacteria are harvested by centrifugation and suspended in 50 ⁇ l of lysis solution (SL: 50 mM Tris-Ci, pH 8.0, 2.5 mM EDTA, 2% SDS, 4 M urea, 0.7 M ⁇ -mercaptoethanol ). After a few minutes at room temperature, 10 ⁇ l are deposited on 16.5% polyacrylamide gel for "Tricine” type electrophoresis [23] which allows the separation of proteins of small molecular mass.
- Panels A, C and E The bacteria were transformed with the plasmids pT7-7, pT7-7-TMEl, pT7-7-TME2
- Panels B, D, F The bacteria were removed at the time indicated in the text and treated as described above. They were then deposited on an electrophoresis gel, ie 16.5% of acrylamide of the "Tricine" type (panel B), or 14% of acrylamide of the Laemmli SDS-PAGE type (panels D and F). The electrophoresis shown in panel F migrated longer than that shown in panel D, this to improve the separation of the bands in the region of 30000 Da. After migration, the gels were stained 10 minutes with Coomassie blue in a solution of 40% methanol, 10% acetic acid, 0.1% Coomassie blue R250, then discolored in a 10% methanol solution, 10% acetic acid, 1% glycerol.
- the expression vectors were constructed as described in Example 1, then introduced into the bacteria BL21 (DE3) pLysS.
- the BL21 (DE3) pLysS bacteria were used for the sake of comparison with the previous experiments because the expression of GST or of its chimeras does not require the DE3-pLysS system.
- Amino acids are indicated with the letter code. The sequence numbering is carried out with respect to the original proteins, GST and viral polyprotein. The one that refers to the membrane domains is shown in italics.
- the panels C and D of Figure 4 attached show the results obtained.
- the growth curves of the bacteria transformed with the different plasmids show that the expression of the chimeras GT1 and GT2 is toxic.
- the expression of TME1 fused to GST is accompanied by the absence of a migrant band at the expected size of 30 kDa. This suggests that a very low level of expression of the chimera is sufficient to kill the bacteria.
- the chimera GST-TME2 is this time visible on the electrophoresis gel, in the zone of molecular mass expected from 30 kDa.
- the level of expression remains limited.
- the protein produced is not soluble despite the presence of GST in the fusion.
- the tests for solubilization, folding and purification of the chimera GST-TME2 were a failure.
- the GST and GST-TME2 proteins were expressed as described in FIG. 4 from 150 ml of culture medium.
- the bacteria were then harvested by centrifugation and suspended 20 mM KPO pH 7.7, 0.1M NaCl, 1 mM EDTA, 1 mM NaN 3 to obtain 100 OD / ml.
- Two ml of each culture are taken for sonication in 30 sec increments at 15% amplitude.
- a sample is taken for electrophoresis. It corresponds to the well "To" in FIG. 6 (corresponding to the "total").
- a first centrifugation at low speed makes it possible to separate the unbroken bacteria and the inclusion bodies from soluble or membrane proteins. These are found in the supernatant and a sample is taken. It corresponds to the well “Surn” in FIG. 6.
- the fraction containing the GST alone is then treated with an affinity resin which makes it possible to fix and then to elute specifically this protein (well “Af” of the GST gel in FIG. 6 ).
- the fraction containing the insoluble protein GST-TME2 is treated either with a mild detergent such as newt X100 (TX100), in the presence or absence of NaCl, either with a more solubilizing but more destructuring detergent such as sarkosyl, before being again diluted in TX100 and passed over affinity resin.
- TX100 newt X100
- sarkosyl a more solubilizing but more destructuring detergent
- the supernatant containing the GST is passed over an agarose-GSH resin capable of fixing the GST. This is then eluted with an excess of GSH (well indicated “Af” of the GST gel in FIG. 6).
- the pellet containing the GST-TME2 fusion is not solubilized in the presence of a mild detergent such as TX100 (with or without added NaCl, well “TX100 +/- NaCl” of the GST-TME2 gel) but it can be solubilized with a more aggressive detergent like sarkosyl.
- a mild detergent which must promote its folding, the protein is not retained on the affinity resin, unlike GST, which suggests that the fusion protein does not can be folded up.
- EXAMPLE 4 Expression of Expression Vectors Coding for the Fusion Proteins Including an Asp-Pro Site and a GST Site
- the construction of the vectors was carried out as described above and for the two vectors coding for the chimeric proteins GST-TME1 and GST-TME2, to give rise to the vectors coding for the chimeric proteins GST-Asp-Pro-TME1 and GST-Asp-Pro-TME2. They are summarized in Table 3 below.
- Amino acids are indicated with the letter code. The sequence numbering is carried out with respect to the original proteins, GST and viral polyprotein. The one that refers to the membrane domains is shown in italics.
- the effect of the addition of the DP dipeptide is as significant as it is unexpected: it amplifies the expression of the domains and suppresses their toxicity.
- This toxicity attenuating effect is not known for DP dipeptide, the only property of which has been reported to date is its ability to be cut by formic acid. The effect being observed on two different peptides and both initially toxic for the bacterium, one can therefore reasonably think that this property can extend to other hydrophobic and toxic peptides.
- the inventors have verified that the site can be effectively cleaved by formic acid: the cut is slow and requires around 7 days at room temperature.
- the expression vectors pET32a-TrX, pET32a-TrX-dp-TME1 and pET32a-TrX-dp-TME2 were constructed as described above and were then introduced into the bacteria BL21 (DE3) pLysS.
- the BL21 (DE3) pLysS bacteria were used for the sake of comparison with the previous experiments because the expression of GST or of its chimeras does not require the DE3-pLysS system. Positive clones were cultured and induced as described above.
- Amino acids are indicated with the letter code. The sequence numbering is carried out with respect to the original proteins, GST and viral polyprotein. The one that refers to membrane domains is shown in italics. "Gend” refers to the C-terminal sequence of GST from the constructs with the plasmid pGEXKT. It corresponds to the primary peptide sequence SDLSGGGGGLVPRGS. The thioredoxin- SDLSGGGGGLVPRGS-DP- (TME1 or TME2) chimeras are shorter than the protein encoded in the original vector because the insertion is carried out immediately after thioredoxin.
- A Bacterial growth was followed by measuring the increase in turbidity of each culture by optical density at 600 nm as a function of time.
- B the bacteria were removed as indicated for FIG. 4. They were then deposited on electrophoresis gel 14% of acrylamide of Laemmli type SDS-PAGE and treated as indicated for FIG. 4.
- the present invention therefore makes it possible to manufacture, by genetic recombination, hydrophobic peptides corresponding to the membrane domains of proteins.
- E1 and E2 of the envelope of the hepatitis C virus the expression of which has been recognized as lethal in the techniques of the prior art.
- the effect being observed on two really different peptides and both initially toxic to the bacteria indicates that the present invention relates to other hydrophobic and toxic peptides.
- the plasmids pT7-7-pt TME ⁇ and pT7- 7-ptME 2 are those which are described in Example 1.
- the plasmids pT7-7-dp-pt TME ⁇ and pT7-7-dp-pt T ME 2 were constructed and cloned into pT7-7 (SEQ ID No. 33) as described in Example 1 but using the Nde I (5 ') and EcoR I (3') sites of the plasmid.
- the upstream oligonucleotides (5 ′) integrate the dp sequence (gacccg) after the 1st methionine (atg).
- the matrices used to generate each DNA were the plasmids pT7-7-pt T MEi and pT7-7-pt T ME 2 - The sequences were checked after cloning.
- the bacterial strain BL21 (DE3) pLysS was transformed either with the plasmid alone or with the different versions of pT7-7 integrating the 4 constructs expressing TME1, M -DP-TME1 ( Figure 7A) or TME2, M-DP- TME2 ( Figure 7B).
- M represents methionine, it is present in the N-terminal position of the peptides when the toxic proteins are produced according to the present invention with the plasmid pT7-7.
- the growth of the different clones was compared after induction by IPTG, according to the protocol identical to the chemical induction described in Example 2, and averaged over the OD values of 4 different clones for each construct.
- FIGS. 7A and 7B show that the bacteria having a plasmid expressing TMEl and TME2 proteins grow slower after induction than the control strain which is transformed with the vector pT7-7 alone. These results show that the strains transformed with the plasmids expressing the versions M-DP-TME1 (SEQ ID No. 50) and M-DP-TME2 (SEQ ID No. 51) according to the invention grow significantly better than those which express TMs without DP. This is true for TMEl and even more clearly for TME2.
- N-terminal insertion of DP in accordance with the present invention surprisingly contributes to a significant reduction in the toxicity of the expression of membrane domains, in particular in the absence of a soluble fusion protein such as GST or thioredoxin.
- Ciccaglione A.R., Marcantonio C, Costantino A., Equestre M., Geraci A. and Rapicetta M. (1998) Virology 250, 1-8;
- Ciccaglione A.R., Marcantonio C, Equestre M., Jones I.M. and Rapicetta M. (1998) Virus Res 55, 157-165;
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JP2020500004A (ja) * | 2016-09-15 | 2020-01-09 | サン ジェノミクス インコーポレイテッド | 試料における1つまたは複数のタイプの微生物の多様な集団からの核酸分子の普遍的抽出方法 |
US10428370B2 (en) * | 2016-09-15 | 2019-10-01 | Sun Genomics, Inc. | Universal method for extracting nucleic acid molecules from a diverse population of one or more types of microbes in a sample |
US11959125B2 (en) | 2016-09-15 | 2024-04-16 | Sun Genomics, Inc. | Universal method for extracting nucleic acid molecules from a diverse population of one or more types of microbes in a sample |
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US5322769A (en) * | 1988-03-11 | 1994-06-21 | Abbott Laboratories | Methods for using CKS fusion proteins |
US6881558B1 (en) * | 2000-06-26 | 2005-04-19 | The Trustees Of The University Of Pennsylvania | Expression system for cloning toxic genes |
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Non-Patent Citations (5)
Title |
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CICCAGLIONE A R ET AL: "Hepatitis C virus E1 protein induces modification of membrane permeability in E. coli cells." VIROLOGY. UNITED STATES 10 OCT 1998, vol. 250, no. 1, 10 octobre 1998 (1998-10-10), pages 1-8, XP001159707 ISSN: 0042-6822 cité dans la demande * |
CICCAGLIONE A R ET AL: "Secretion and purification of HCV E1 protein forms as glutathione-S-transferase fusion in the baculovirus insect cell system." VIRUS RESEARCH. NETHERLANDS JUN 1998, vol. 55, no. 2, juin 1998 (1998-06), pages 157-165, XP001159708 ISSN: 0168-1702 cité dans la demande * |
DATABASE TREMBL [en ligne] 1 novembre 1996 (1996-11-01) OKAMOTO,H ET AL.: "NS2A protein (Genome polyprotein) (Fragment)." retrieved from EBI Database accession no. Q81569 XP002244032 * |
DATABASE TREMBL [en ligne] 19 décembre 2001 (2001-12-19) SARRAZIN,C ET AL.: "Envelope protein (Genome polyprotein) (Fragment). E2" retrieved from EBI Database accession no. Q91EE4 XP002244033 * |
WANG YONGBAO ET AL: "A unique approach for high level expression and production of a recombinant cobra neurotoxin in Escherichia coli." JOURNAL OF BIOTECHNOLOGY. NETHERLANDS 11 APR 2002, vol. 94, no. 3, 11 avril 2002 (2002-04-11), pages 235-244, XP001159705 ISSN: 0168-1656 * |
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FR2859221A1 (fr) * | 2003-08-28 | 2005-03-04 | Centre Nat Rech Scient | Vecteur de co-expression de domaines membranaires de proteines d'enveloppe d'un virus et utilisations |
WO2005024031A1 (fr) * | 2003-08-28 | 2005-03-17 | Centre National De La Recherche Scientifique | Vecteur de co-expression de domaines membranaires de proteines d'enveloppe d'un virus et utilisations |
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US20060173165A1 (en) | 2006-08-03 |
FR2844806B1 (fr) | 2007-06-15 |
US7544775B2 (en) | 2009-06-09 |
AU2003276359A1 (en) | 2004-04-08 |
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