WO2004015117A3 - Targeted gene modification by single-stranded dna oligonucleotides - Google Patents

Targeted gene modification by single-stranded dna oligonucleotides Download PDF

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Publication number
WO2004015117A3
WO2004015117A3 PCT/IB2003/003699 IB0303699W WO2004015117A3 WO 2004015117 A3 WO2004015117 A3 WO 2004015117A3 IB 0303699 W IB0303699 W IB 0303699W WO 2004015117 A3 WO2004015117 A3 WO 2004015117A3
Authority
WO
WIPO (PCT)
Prior art keywords
desired alteration
target genomic
stranded dna
targeted gene
dna
Prior art date
Application number
PCT/IB2003/003699
Other languages
French (fr)
Other versions
WO2004015117A2 (en
Inventor
Riele Henricus Petrus Josep Te
Original Assignee
Nl Kanker I
Riele Henricus Petrus Josep Te
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nl Kanker I, Riele Henricus Petrus Josep Te filed Critical Nl Kanker I
Priority to AU2003253197A priority Critical patent/AU2003253197A1/en
Publication of WO2004015117A2 publication Critical patent/WO2004015117A2/en
Publication of WO2004015117A3 publication Critical patent/WO2004015117A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Mycology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A method of site-specifically modifying in a cell a target genomic DNA sequence consisting of first and second DNA strands to introduce a desired alteration, which desired alteration is of a single base-pair or a few base-pairs and is base-pair insertion, deletion, substitution or any combination thereof, the method comprising introducing into the cell a single-stranded targeting DNA molecule, wherein the targeting DNA molecule is 10-100 nucleotides in length and is of nucleotide sequence identical to nucleotide sequence within the first or second DNA strand of the target genomic DNA sequence except for the desired alteration and optionally one or more additional differences, wherein the differences are each insertion, substitution or deletion of a nucleotide, resulting in site-specific modification of the target genomic DNA to introduce the desired alteration.
PCT/IB2003/003699 2002-08-13 2003-08-13 Targeted gene modification by single-stranded dna oligonucleotides WO2004015117A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003253197A AU2003253197A1 (en) 2002-08-13 2003-08-13 Targeted gene modification by single-stranded dna oligonucleotides

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0218803A GB0218803D0 (en) 2002-08-13 2002-08-13 Targeted gene modification by single-standed DNA oligonucleotides
GB0218803.5 2002-08-13

Publications (2)

Publication Number Publication Date
WO2004015117A2 WO2004015117A2 (en) 2004-02-19
WO2004015117A3 true WO2004015117A3 (en) 2004-06-03

Family

ID=9942224

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2003/003699 WO2004015117A2 (en) 2002-08-13 2003-08-13 Targeted gene modification by single-stranded dna oligonucleotides

Country Status (3)

Country Link
AU (1) AU2003253197A1 (en)
GB (1) GB0218803D0 (en)
WO (1) WO2004015117A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011058555A1 (en) 2009-11-12 2011-05-19 Yeda Research And Development Co. Ltd. A method of editing dna in a cell and constructs capable of same
WO2014102688A1 (en) * 2012-12-27 2014-07-03 Cellectis New design matrix for improvement of homology-directed gene targeting
EP3498837A4 (en) * 2016-08-12 2020-04-15 Nexuspiral Inc. Genome editing method
WO2021186913A1 (en) * 2020-03-20 2021-09-23 国立大学法人筑波大学 Method for modifying methylated state of genomic dna
EP4349983A1 (en) * 2021-05-27 2024-04-10 National Institute Of Advanced Industrial Science and Technology Genome editing method

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990014092A1 (en) * 1989-05-18 1990-11-29 Cell Genesys, Inc. Single-strand site-directed modification of mammalian genes in vivo
WO1993004169A1 (en) * 1991-08-20 1993-03-04 Genpharm International, Inc. Gene targeting in animal cells using isogenic dna constructs
WO1994004032A1 (en) * 1992-08-21 1994-03-03 The Regents Of The University Of California Composition and method for altering dna sequences by homologous recombination
WO1996040271A1 (en) * 1995-06-07 1996-12-19 Yale University Treatment of hemoglobinopathies
WO1997005268A1 (en) * 1995-07-26 1997-02-13 Setratech Homologous recombination in mismatch repair inactivated eukaryotic cells
WO2000009755A2 (en) * 1998-08-12 2000-02-24 Pangene Corporation Domain specific gene evolution
WO2001015740A1 (en) * 1999-08-27 2001-03-08 Valigen (Us), Inc. Single-stranded oligodeoxynucleotide mutational vectors
EP1152058A1 (en) * 2000-05-03 2001-11-07 Institut Curie Methods and compositions for effecting homologous recombination
WO2002026967A2 (en) * 2000-09-25 2002-04-04 Thomas Jefferson University Targeted gene correction by single-stranded oligodeoxynucleotides

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990014092A1 (en) * 1989-05-18 1990-11-29 Cell Genesys, Inc. Single-strand site-directed modification of mammalian genes in vivo
WO1993004169A1 (en) * 1991-08-20 1993-03-04 Genpharm International, Inc. Gene targeting in animal cells using isogenic dna constructs
WO1994004032A1 (en) * 1992-08-21 1994-03-03 The Regents Of The University Of California Composition and method for altering dna sequences by homologous recombination
WO1996040271A1 (en) * 1995-06-07 1996-12-19 Yale University Treatment of hemoglobinopathies
WO1997005268A1 (en) * 1995-07-26 1997-02-13 Setratech Homologous recombination in mismatch repair inactivated eukaryotic cells
WO2000009755A2 (en) * 1998-08-12 2000-02-24 Pangene Corporation Domain specific gene evolution
WO2001015740A1 (en) * 1999-08-27 2001-03-08 Valigen (Us), Inc. Single-stranded oligodeoxynucleotide mutational vectors
EP1152058A1 (en) * 2000-05-03 2001-11-07 Institut Curie Methods and compositions for effecting homologous recombination
WO2002026967A2 (en) * 2000-09-25 2002-04-04 Thomas Jefferson University Targeted gene correction by single-stranded oligodeoxynucleotides

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
CAMPBELL C R ET AL: "HOMOLOGOUS RECOMBINATION INVOLVING SMALL SINGLE-STRANDED OLIGONUCLEOTIDES IN HUMAN CELLS", THE NEW BIOLOGIST, PHILADELPHIA,PA, US, vol. 1, no. 2, November 1989 (1989-11-01), pages 223 - 227, XP002933043, ISSN: 1043-4674 *
CULVER KENNETH W ET AL: "Correction of chromosomal point mutations in human cells with bifunctional oligonucleotides", NATURE BIOTECHNOLOGY, NATURE PUBLISHING, US, vol. 17, no. 10, October 1999 (1999-10-01), pages 989 - 993, XP002151266, ISSN: 1087-0156 *
GAMPER H B ET AL: "Strand invasion of supercoiled DNA by oligonucleotides with a triplex guided sequence", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, AMERICAN CHEMICAL SOCIETY, WASHINGTON, DC, US, vol. 120, 1998, pages 2182 - 2183, XP002151265, ISSN: 0002-7863 *
MOERSCHELL R P ET AL: "TRANSFORMATION OF YEAST WITH SYNTHETIC OLIGONUCLEOTIDES", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 85, January 1988 (1988-01-01), pages 524 - 528, XP002933044, ISSN: 0027-8424 *

Also Published As

Publication number Publication date
AU2003253197A8 (en) 2004-02-25
GB0218803D0 (en) 2002-09-18
WO2004015117A2 (en) 2004-02-19
AU2003253197A1 (en) 2004-02-25

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