JP2005517433A5 - - Google Patents
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- JP2005517433A5 JP2005517433A5 JP2003569790A JP2003569790A JP2005517433A5 JP 2005517433 A5 JP2005517433 A5 JP 2005517433A5 JP 2003569790 A JP2003569790 A JP 2003569790A JP 2003569790 A JP2003569790 A JP 2003569790A JP 2005517433 A5 JP2005517433 A5 JP 2005517433A5
- Authority
- JP
- Japan
- Prior art keywords
- sina molecule
- nucleotide
- nucleotides
- fragment
- molecule
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 125000003729 nucleotide group Chemical group 0.000 claims 23
- 239000002773 nucleotide Substances 0.000 claims 19
- 230000000692 anti-sense Effects 0.000 claims 13
- 150000007523 nucleic acids Chemical class 0.000 claims 5
- 108020004707 nucleic acids Proteins 0.000 claims 5
- 239000002213 purine nucleotide Substances 0.000 claims 5
- 206010049460 Abasia Diseases 0.000 claims 4
- 102000000852 Tumor Necrosis Factor-alpha Human genes 0.000 claims 4
- 108010001801 Tumor Necrosis Factor-alpha Proteins 0.000 claims 4
- 125000000561 purinyl group Chemical class N1=C(N=C2N=CNC2=C1)* 0.000 claims 4
- 229920000160 (ribonucleotides)n+m Polymers 0.000 claims 3
- 239000002719 pyrimidine nucleotide Substances 0.000 claims 3
- 150000003230 pyrimidines Chemical class 0.000 claims 3
- 229920002676 Complementary DNA Polymers 0.000 claims 2
- 229920001850 Nucleic acid sequence Polymers 0.000 claims 2
- 230000025458 RNA interference Effects 0.000 claims 2
- 229920001914 Ribonucleotide Polymers 0.000 claims 2
- 230000000295 complement Effects 0.000 claims 2
- 125000003976 glyceryl group Chemical group [H]C([*])([H])C(O[H])([H])C(O[H])([H])[H] 0.000 claims 2
- 239000002336 ribonucleotide Substances 0.000 claims 2
- 125000002652 ribonucleotide group Chemical group 0.000 claims 2
- 230000002987 rna-interference Effects 0.000 claims 2
- RYYWUUFWQRZTIU-UHFFFAOYSA-K thiophosphate Chemical compound [O-]P([O-])([O-])=S RYYWUUFWQRZTIU-UHFFFAOYSA-K 0.000 claims 2
- IQFYYKKMVGJFEH-XLPZGREQSA-N DEOXYTHYMIDINE Chemical group O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 claims 1
- 240000003247 Erythrina poeppigiana Species 0.000 claims 1
- 229920000272 Oligonucleotide Polymers 0.000 claims 1
- 235000009776 Rathbunia alamosensis Nutrition 0.000 claims 1
- 229920000972 Sense strand Polymers 0.000 claims 1
- 239000000969 carrier Substances 0.000 claims 1
- 238000003776 cleavage reaction Methods 0.000 claims 1
- 239000003085 diluting agent Substances 0.000 claims 1
- 230000002452 interceptive Effects 0.000 claims 1
- 230000004048 modification Effects 0.000 claims 1
- 238000006011 modification reaction Methods 0.000 claims 1
- 239000008194 pharmaceutical composition Substances 0.000 claims 1
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 claims 1
- 229920000023 polynucleotide Polymers 0.000 claims 1
- 239000002157 polynucleotide Substances 0.000 claims 1
Claims (34)
a.第1の核酸鎖は第2の鎖に対して相補性を有しており;
b.前記siNA分子の各鎖は19−29ヌクレオチドの長さであり;そして
c.前記siNA分子はセンス鎖の5’末端および/または3’末端およびアンチセンス鎖の3’末端に少なくとも1つの化学的に修飾されたヌクレオチドまたは非ヌクレオチドを含む,
ことを特徴とするsiNA分子。 A chemically synthesized double stranded short interfering nucleic acid (siNA) molecule that directs cleavage of tumor necrosis factor (TNF) RNA by RNA interference (RNAi), essentially consisting of a first nucleic acid strand and a second nucleic acid strand Of nucleic acid strands, where
a. The first nucleic acid strand is complementary to the second strand;
b. Each strand of the siNA molecule is 19-29 nucleotides in length; and c. The siNA molecule comprises at least one chemically modified nucleotide or non-nucleotide at the 5 ′ end and / or the 3 ′ end of the sense strand and the 3 ′ end of the antisense strand;
SiNA molecules characterized in that.
Applications Claiming Priority (9)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US35858002P | 2002-02-20 | 2002-02-20 | |
US36312402P | 2002-03-11 | 2002-03-11 | |
US38678202P | 2002-06-06 | 2002-06-06 | |
US40678402P | 2002-08-29 | 2002-08-29 | |
US40837802P | 2002-09-05 | 2002-09-05 | |
US40929302P | 2002-09-09 | 2002-09-09 | |
US42935902P | 2002-11-26 | 2002-11-26 | |
US44012903P | 2003-01-15 | 2003-01-15 | |
PCT/US2003/004741 WO2003070897A2 (en) | 2002-02-20 | 2003-02-20 | RNA INTERFERENCE MEDIATED INHIBITION OF TNF AND TNF RECEPTOR SUPERFAMILY GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA) |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2005517433A JP2005517433A (en) | 2005-06-16 |
JP2005517433A5 true JP2005517433A5 (en) | 2006-03-30 |
Family
ID=27761726
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2003569790A Pending JP2005517433A (en) | 2002-02-20 | 2003-02-20 | RNA interference-mediated inhibition of TNF and TNF receptor superfamily gene expression using short interfering nucleic acids (siNA) |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP1478730A4 (en) |
JP (1) | JP2005517433A (en) |
AU (1) | AU2003217550A1 (en) |
CA (1) | CA2477014A1 (en) |
WO (1) | WO2003070897A2 (en) |
Families Citing this family (43)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU2001257526B2 (en) | 2000-05-04 | 2007-07-19 | Avi Biopharma, Inc. | Splice-region antisense composition and method |
US9657294B2 (en) | 2002-02-20 | 2017-05-23 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA) |
US9181551B2 (en) | 2002-02-20 | 2015-11-10 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA) |
US7951935B2 (en) | 2002-11-14 | 2011-05-31 | Dharmacon, Inc. | siRNA targeting v-myc myelocytomatosis viral oncogene homolog (MYC) |
WO2006006948A2 (en) | 2002-11-14 | 2006-01-19 | Dharmacon, Inc. | METHODS AND COMPOSITIONS FOR SELECTING siRNA OF IMPROVED FUNCTIONALITY |
US9839649B2 (en) | 2002-11-14 | 2017-12-12 | Thermo Fisher Scientific Inc. | Methods and compositions for selecting siRNA of improved functionality |
US9879266B2 (en) | 2002-11-14 | 2018-01-30 | Thermo Fisher Scientific Inc. | Methods and compositions for selecting siRNA of improved functionality |
US9228186B2 (en) | 2002-11-14 | 2016-01-05 | Thermo Fisher Scientific Inc. | Methods and compositions for selecting siRNA of improved functionality |
US7977471B2 (en) | 2002-11-14 | 2011-07-12 | Dharmacon, Inc. | siRNA targeting TNFα |
US7635770B2 (en) | 2002-11-14 | 2009-12-22 | Dharmacon, Inc. | siRNA targeting protein kinase N-3 (PKN-3) |
US9719094B2 (en) | 2002-11-14 | 2017-08-01 | Thermo Fisher Scientific Inc. | RNAi targeting SEC61G |
US7612196B2 (en) | 2002-11-14 | 2009-11-03 | Dharmacon, Inc. | siRNA targeting cyclin-dependent kinase inhibitor 1B (p27, Kip1) (CDKN1B) |
US9719092B2 (en) | 2002-11-14 | 2017-08-01 | Thermo Fisher Scientific Inc. | RNAi targeting CNTD2 |
US10011836B2 (en) | 2002-11-14 | 2018-07-03 | Thermo Fisher Scientific Inc. | Methods and compositions for selecting siRNA of improved functionality |
US7619081B2 (en) | 2002-11-14 | 2009-11-17 | Dharmacon, Inc. | siRNA targeting coatomer protein complex, subunit beta 2 (COPB2) |
US8198427B1 (en) | 2002-11-14 | 2012-06-12 | Dharmacon, Inc. | SiRNA targeting catenin, beta-1 (CTNNB1) |
EP2284266B1 (en) | 2002-11-14 | 2013-11-06 | Thermo Fisher Scientific Biosciences Inc. | siRNA targeting tp53 |
US7691998B2 (en) | 2002-11-14 | 2010-04-06 | Dharmacon, Inc. | siRNA targeting nucleoporin 62kDa (Nup62) |
US7592442B2 (en) | 2002-11-14 | 2009-09-22 | Dharmacon, Inc. | siRNA targeting ribonucleotide reductase M2 polypeptide (RRM2 or RNR-R2) |
US9771586B2 (en) | 2002-11-14 | 2017-09-26 | Thermo Fisher Scientific Inc. | RNAi targeting ZNF205 |
US20050191653A1 (en) * | 2003-11-03 | 2005-09-01 | Freier Susan M. | Modulation of SGLT2 expression |
WO2005117991A2 (en) * | 2004-05-04 | 2005-12-15 | Nastech Pharmaceutical Company Inc. | Compositions and methods for enhancing delivery of nucleic acids into cells and for modifying expression of target genes in cells |
US20060040882A1 (en) * | 2004-05-04 | 2006-02-23 | Lishan Chen | Compostions and methods for enhancing delivery of nucleic acids into cells and for modifying expression of target genes in cells |
US7605250B2 (en) | 2004-05-12 | 2009-10-20 | Dharmacon, Inc. | siRNA targeting cAMP-specific phosphodiesterase 4D |
US20060253100A1 (en) | 2004-10-22 | 2006-11-09 | Medtronic, Inc. | Systems and Methods to Treat Pain Locally |
US20060189564A1 (en) * | 2004-10-22 | 2006-08-24 | Medtronic, Inc. | Methods and sequences to suppress pro-inflamatory cytokine actions locally to treat pain |
EP1896586A2 (en) | 2005-06-28 | 2008-03-12 | Medtronic, Inc. | Methods and sequences to preferentially suppress expression of mutated huntingtin |
AU2006287481A1 (en) * | 2005-09-08 | 2007-03-15 | Mdrna, Inc. | Pharmaceutical compositions for delivery of ribonucleic acid to a cell |
EP1942943A2 (en) * | 2005-11-04 | 2008-07-16 | Nastech Pharmaceutical Company Inc. | Peptide-dicer substrate rna conjugates as delivery vehicles for sirna |
US7785834B2 (en) | 2005-11-10 | 2010-08-31 | Ercole Biotech, Inc. | Soluble TNF receptors and their use in treatment of disease |
US8138160B2 (en) * | 2006-08-03 | 2012-03-20 | Warsaw Orthopedic, Inc. | Reagents, methods and systems to suppress pro-inflammatory cytokines |
WO2008109369A2 (en) * | 2007-03-02 | 2008-09-12 | Mdrna, Inc. | Nucleic acid compounds for inhibiting tnf gene expression and uses thereof |
CN104278033A (en) | 2007-05-01 | 2015-01-14 | 桑塔里斯制药公司 | Splice Switching Oligomers for TNF Superfamily Receptors and their Use in Treatment of Disease |
EP3434259A1 (en) | 2007-05-04 | 2019-01-30 | Marina Biotech, Inc. | Amino acid lipids and uses thereof |
US20090036396A1 (en) * | 2007-08-03 | 2009-02-05 | Alcon Research, Ltd. | RNAi-RELATED INHIBITION OF TNFalpha SIGNALING PATHWAY FOR TREATMENT OF OCULAR ANGIOGENESIS |
WO2009033027A2 (en) | 2007-09-05 | 2009-03-12 | Medtronic, Inc. | Suppression of scn9a gene expression and/or function for the treatment of pain |
USRE48948E1 (en) | 2008-04-18 | 2022-03-01 | Warsaw Orthopedic, Inc. | Clonidine compounds in a biodegradable polymer |
KR20110071017A (en) | 2008-10-16 | 2011-06-27 | 마리나 바이오테크, 인크. | Processes and compositions for liposomal and efficient delivery of gene silencing therapeutics |
WO2010096813A1 (en) | 2009-02-23 | 2010-08-26 | New York Blood Center | Krüppel-like factors and fat regulation |
US20100239632A1 (en) | 2009-03-23 | 2010-09-23 | Warsaw Orthopedic, Inc. | Drug depots for treatment of pain and inflammation in sinus and nasal cavities or cardiac tissue |
EP2609198B8 (en) | 2010-08-24 | 2018-03-28 | Sirna Therapeutics, Inc. | SINGLE-STRANDED RNAi AGENTS CONTAINING AN INTERNAL, NON-NUCLEIC ACID SPACER |
WO2012058210A1 (en) | 2010-10-29 | 2012-05-03 | Merck Sharp & Dohme Corp. | RNA INTERFERENCE MEDIATED INHIBITION OF GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACIDS (siNA) |
PL226431B1 (en) | 2013-08-23 | 2017-07-31 | Inst Biochemii I Biofizyki Polskiej Akademii Nauk | Particle miR172 of vegetal origin or its synthetic equivalent, method�for decreasing proliferation of lymphocytes B and application of particle miR173 |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0649467B1 (en) * | 1992-07-02 | 1998-09-16 | HYBRIDON, Inc. | Self-stabilized oligonucleotides as therapeutic agents |
JPH08505521A (en) * | 1992-11-03 | 1996-06-18 | ジーン・シアーズ・プロプライアタリー・リミテッド | TNF-α Ribozyme and Degradation-Resistant mRNA Derivative Linked to TNF-α Ribozyme |
US6506569B1 (en) * | 1997-05-30 | 2003-01-14 | Human Genome Sciences, Inc. | Antibodies to human tumor necrosis factor receptor TR10 |
US6194151B1 (en) * | 1997-09-26 | 2001-02-27 | Millenium Pharmaceuticals, Inc. | Molecules of the TNF receptor superfamily and uses therefor |
WO1999027086A1 (en) * | 1997-11-25 | 1999-06-03 | Brax Group Limited | Chimeric antisense oligonucleotides against tnf-alpha and their uses |
US6506559B1 (en) * | 1997-12-23 | 2003-01-14 | Carnegie Institute Of Washington | Genetic inhibition by double-stranded RNA |
US6007995A (en) * | 1998-06-26 | 1999-12-28 | Isis Pharmaceuticals Inc. | Antisense inhibition of TNFR1 expression |
DE19956568A1 (en) * | 1999-01-30 | 2000-08-17 | Roland Kreutzer | Method and medicament for inhibiting the expression of a given gene |
ES2728168T3 (en) * | 2000-12-01 | 2019-10-22 | Max Planck Gesellschaft | Small RNA molecules that mediate RNA interference |
-
2003
- 2003-02-20 CA CA002477014A patent/CA2477014A1/en not_active Abandoned
- 2003-02-20 WO PCT/US2003/004741 patent/WO2003070897A2/en active Application Filing
- 2003-02-20 AU AU2003217550A patent/AU2003217550A1/en not_active Abandoned
- 2003-02-20 EP EP03713502A patent/EP1478730A4/en not_active Withdrawn
- 2003-02-20 JP JP2003569790A patent/JP2005517433A/en active Pending
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