WO2004007706A8 - Azucar-nucleótido pirofosfatasa/fosfodiesterasa (nppasa) vegetal, procedimiento de obtención, uso en la fabricación de dispositivos de ensayo y su utilización en la obtención de plantas transgénicas - Google Patents

Azucar-nucleótido pirofosfatasa/fosfodiesterasa (nppasa) vegetal, procedimiento de obtención, uso en la fabricación de dispositivos de ensayo y su utilización en la obtención de plantas transgénicas

Info

Publication number
WO2004007706A8
WO2004007706A8 PCT/ES2003/000363 ES0300363W WO2004007706A8 WO 2004007706 A8 WO2004007706 A8 WO 2004007706A8 ES 0300363 W ES0300363 W ES 0300363W WO 2004007706 A8 WO2004007706 A8 WO 2004007706A8
Authority
WO
WIPO (PCT)
Prior art keywords
nppase
production
same
phosphosulphate
sugar
Prior art date
Application number
PCT/ES2003/000363
Other languages
English (en)
French (fr)
Other versions
WO2004007706A1 (es
Inventor
Perez Francisco Jose Munoz
Lopez Milagros Rodriguez
Fernandez Miren Edurne Baroja
Romero Francisco Javie Pozueta
Toshiaki Mitsui
Yohei Nanjo
Original Assignee
Univ Navarra Publica
Perez Francisco Jose Munoz
Lopez Milagros Rodriguez
Fernandez Miren Edurne Baroja
Romero Francisco Javie Pozueta
Toshiaki Mitsui
Yohei Nanjo
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Navarra Publica, Perez Francisco Jose Munoz, Lopez Milagros Rodriguez, Fernandez Miren Edurne Baroja, Romero Francisco Javie Pozueta, Toshiaki Mitsui, Yohei Nanjo filed Critical Univ Navarra Publica
Priority to MXPA05000599A priority Critical patent/MXPA05000599A/es
Priority to BR0312665-0A priority patent/BR0312665A/pt
Priority to US10/520,696 priority patent/US20060242739A1/en
Priority to JP2004520690A priority patent/JP2005532806A/ja
Priority to EP03740519A priority patent/EP1535998A1/en
Priority to AU2003281068A priority patent/AU2003281068A1/en
Publication of WO2004007706A1 publication Critical patent/WO2004007706A1/es
Publication of WO2004007706A8 publication Critical patent/WO2004007706A8/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Electrostatic Separation (AREA)

Abstract

La NPPasa es un enzima que cataliza la hidrólisis de una amplia gama de pequeñas moléculas con enlaces fosfodiéster y fosfosulfato, entre las que destaca la ADPG (adenosina difosfato glucosa) y el APS (adenosina 5'fosfosulfato). El enzima obtenido a partir de extractos vegetales se utiliza en dispositivos de ensayo para determinar niveles de azúcares-nucleósido-difosfato, basados bien en el azúcar-1-fosfato liberado, bien en el nucleósido monofosfato productos formados ambos por la reacción catalizada por la NPPasa, así como la detección de sulfonucleótidos como el 3'-fosfoadenosina 5'fosfosulfato (PAPS) y el APS. Asimismo se describe la secuencia aminoacídica del enzima, así como la secuencia nucleotídica de un cDNA completo y otro incompleto. Por último se describe la obtención de plantas transgénicas que sobre-expresen NPPasa y que poseen un contenido alto en azúcares, bajo contenido en almidón y polisacáridos de pared celular y una alta resistencia a altas concentraciones de sales y alta temperatura.
PCT/ES2003/000363 2002-07-15 2003-07-15 Azucar-nucleótido pirofosfatasa/fosfodiesterasa (nppasa) vegetal, procedimiento de obtención, uso en la fabricación de dispositivos de ensayo y su utilización en la obtención de plantas transgénicas WO2004007706A1 (es)

Priority Applications (6)

Application Number Priority Date Filing Date Title
MXPA05000599A MXPA05000599A (es) 2002-07-15 2003-07-15 Azucar-nucleotido pirofosfatasa/fosfodiesterasa (nppsas) vegetal, procedimiento de obtencion, uso en la fabricacion de dispositivos de ensayo y su utilizacion en la obtencion de plantas transgenicas.
BR0312665-0A BR0312665A (pt) 2002-07-15 2003-07-15 Pirofosfatase/fosfodiesterase (nppase) de açúcar nucleotìdeo vegetal, método de produção, uso na fabricação de dispositivo de teste e sua aplicação na produção de plantas transgênicas
US10/520,696 US20060242739A1 (en) 2002-07-15 2003-07-15 Plant nucleotide-sugar pyrophosphatase/phosphodiesterase (nppase), method of obtaining same and use of same in the production of assay devices and in the production of transgenic plants
JP2004520690A JP2005532806A (ja) 2002-07-15 2003-07-15 植物ヌクレオチド糖ピロフォスファターゼ／フォスフォジエステラーゼ（ＮＰＰａｓｅ）、産生方法、試験装置の製造における使用および形質転換植物の産生におけるその応用
EP03740519A EP1535998A1 (en) 2002-07-15 2003-07-15 Plant nucleotide-sugar pyrophosphatase/phosphodiesterase (nppase), method of obtaining same and use of same in the production of assay devices and in the production of transgenic plants
AU2003281068A AU2003281068A1 (en) 2002-07-15 2003-07-15 Plant nucleotide-sugar pyrophosphatase/phosphodiesterase (nppase), method of obtaining same and use of same in the production of assay devices and in the production of transgenic plants

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
ES200201647 2002-07-15
ESP200201647 2002-07-15
ESP200202673 2002-11-20
ES200202673 2002-11-20

Publications (2)

Publication Number Publication Date
WO2004007706A1 WO2004007706A1 (es) 2004-01-22
WO2004007706A8 true WO2004007706A8 (es) 2004-04-29

Family

ID=30116973

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/ES2003/000363 WO2004007706A1 (es) 2002-07-15 2003-07-15 Azucar-nucleótido pirofosfatasa/fosfodiesterasa (nppasa) vegetal, procedimiento de obtención, uso en la fabricación de dispositivos de ensayo y su utilización en la obtención de plantas transgénicas

Country Status (8)

Country Link
US (1) US20060242739A1 (es)
EP (1) EP1535998A1 (es)
JP (1) JP2005532806A (es)
CN (1) CN1668739A (es)
AU (1) AU2003281068A1 (es)
BR (1) BR0312665A (es)
MX (1) MXPA05000599A (es)
WO (1) WO2004007706A1 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006000066A2 (en) * 2004-06-29 2006-01-05 Puratos Naamloze Vennootschap Dough comprising nucleotide pyrophosphatase inhibitor
EP1771073B1 (en) 2004-06-29 2018-09-05 Puratos Naamloze Vennootschap Dough comprising nucleotide pyrophosphatase inhibitor
ES2354895B1 (es) * 2008-09-12 2012-01-23 Iden Carbohydrate Biotechnology, S.L Procedimiento para la producción de plantas transgénicas que presentan alto contenido y rendimiento en almidón y alto balance amilosa/amilopectina.
EP2449099A2 (en) * 2009-06-30 2012-05-09 E. I. du Pont de Nemours and Company Plant seeds with altered storage compound levels, related constructs and methods involving genes encoding cytosolic pyrophosphatase
CN102676556A (zh) * 2012-05-15 2012-09-19 四川农业大学 一种来源于新疆沙冬青的i型液泡膜焦磷酸酶的基因序列及其克隆方法
CN104946612B (zh) * 2015-07-15 2016-11-16 北京中科紫鑫科技有限责任公司 一种分离纯化无机焦磷酸酶的方法
CN106755022B (zh) * 2015-11-25 2020-08-04 中国科学院大连化学物理研究所 乙酰葡萄糖胺磷酸变位酶AtAGM编码基因及其酶、制备、应用与酶活性检测方法
CN113481180A (zh) * 2021-07-05 2021-10-08 吉林大学 碱性嗜热无机焦磷酸酶及其在增强聚合酶链式反应和合成udp-半乳糖反应中的应用

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5792920A (en) * 1988-11-10 1998-08-11 Imperial Chemical Industries Plc Plants with altered ability to synthesize starch and process for obtaining them
WO1995019570A1 (en) * 1994-01-14 1995-07-20 Genentech, Inc. Antagonists to insulin receptor tyrosine kinase inhibitor
US6329204B1 (en) * 1999-02-10 2001-12-11 E. I. Du Pont De Nemours & Company Plant caffeic acid 3-0-methyltransferase homologs
AU2001230263B2 (en) * 2000-02-02 2006-12-07 Universidad Publica De Navarra Plant adpglucose pyrophosphatase, method for the production thereof, its use in the production of assay devices and for obtaining transgenic plants

Also Published As

Publication number Publication date
JP2005532806A (ja) 2005-11-04
WO2004007706A1 (es) 2004-01-22
US20060242739A1 (en) 2006-10-26
AU2003281068A1 (en) 2004-02-02
MXPA05000599A (es) 2005-08-29
BR0312665A (pt) 2005-04-26
CN1668739A (zh) 2005-09-14
EP1535998A1 (en) 2005-06-01

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