WO2004005450A3 - Verfahren zur produktion von s-adenosyl-l-methionin durch fermentation von genetisch modifizierten mikroorganismen - Google Patents

Verfahren zur produktion von s-adenosyl-l-methionin durch fermentation von genetisch modifizierten mikroorganismen Download PDF

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Publication number
WO2004005450A3
WO2004005450A3 PCT/EP2003/007087 EP0307087W WO2004005450A3 WO 2004005450 A3 WO2004005450 A3 WO 2004005450A3 EP 0307087 W EP0307087 W EP 0307087W WO 2004005450 A3 WO2004005450 A3 WO 2004005450A3
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WIPO (PCT)
Prior art keywords
production strain
sam
cells
methionine
producing
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PCT/EP2003/007087
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English (en)
French (fr)
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WO2004005450A2 (de
Inventor
Juergen Philipp Baur
Sandra Weich
Sandra Bettermann
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Axaron Bioscience Ag
Juergen Philipp Baur
Sandra Weich
Sandra Bettermann
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Publication date
Application filed by Axaron Bioscience Ag, Juergen Philipp Baur, Sandra Weich, Sandra Bettermann filed Critical Axaron Bioscience Ag
Priority to AU2003246362A priority Critical patent/AU2003246362A1/en
Publication of WO2004005450A2 publication Critical patent/WO2004005450A2/de
Publication of WO2004005450A3 publication Critical patent/WO2004005450A3/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/38—Nucleosides
    • C12P19/40—Nucleosides having a condensed ring system containing a six-membered ring having two nitrogen atoms in the same ring, e.g. purine nucleosides

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Die Erfindung betrifft ein Verfahren zur Herstellung von S-Adenosyl-Methionin (SAM) in einem Produktionsstamm mit den folgenden Schritten: a) Durchführen des Anreicherungsprozesses, wobei der Produktionsstamm unter solchen Bedingungen kultiviert wird, die zu einer Vermehrung des Produktionstamrnes und die, gegebenenfalls nach Zugabe von Methionin, zu einer SAM-Anreicherung in den Zellen der Produktionsstamm-Kultur führen, b) Durchführen des SAM-Fermentationsprozesses, wobei die Produktionsstamm-Kultur aus a), gegebenenfalls unter Zugabe von Methionin, unter solchen Bedingungen kultiviert wird, die in den Zellen des Produktionsstammes im wesentlichen zur Anreicherung von SAM führen, c) gegebenenfalls Isolieren des in den Zellen des Produktionsstammes angereicherten SAM durch Aufschluss der Zellen und gegebenenfalls Aufreinigung des isolierten SAM, bei dem der Produktionsstamm ein prokaryontischer oder eukaryontischer Organismus ist, der mindestens eines der Gene MUP1, MUP3, SAM3, MET28, BAS1, PH02, GCN4, COQS, SIP18 oder eines seiner Homologen im Vergleich zum Wildtyp-Produktionsstamm überexprimiert oder in dem mindestens eine Kopie mindestens eines der Gene MIG1, SPE2, 13103, ABD1, ERG6, STE14, PEM1, CTM1, HSL7, HMT1, NOP2, DIM1, PET56, ALD6 und SNQ 1 inaktiviert ist oder der eine Kombination dieser Überexpressionen und Geninaktivierungen aufweist. Die Erfindung betrifft weiterhin einen prokaryontischen oder eukaryontischen Organismus, der mindestens eines der Gene ausgewählt aus der Gruppe MUP1, MUP3, SAM3, MET28, BAS1, PH02, GCN4, COQS, SIP18 im Vergleich zum Wildtyp-Organismus überexprimiert und/oder in dem mindestens eine Kopie mindestens eines der Gene MIG1, SAM3, SPE2, BI03, ABD1, ERG6, STE14, PEM1, CTM1, HSL7, HMT1, NOP2, D1M1, PET56, ALD6 und SNQ 1 inaktiviert ist, sowie die Verwendung dieser Organismen zur Herstellung von SAM. Einen weiteren Gegenstand der Erfindung stellt die Verwendung der vorstehend genannten Organismen zur Produktion von SAM dar.
PCT/EP2003/007087 2002-07-04 2003-07-03 Verfahren zur produktion von s-adenosyl-l-methionin durch fermentation von genetisch modifizierten mikroorganismen WO2004005450A2 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003246362A AU2003246362A1 (en) 2002-07-04 2003-07-03 Method for producing s-adenosyl-l-methionine by fermenting genetically modified microorganisms

Applications Claiming Priority (2)

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DE10230224.3 2002-07-04
DE2002130224 DE10230224A1 (de) 2002-07-04 2002-07-04 Verfahren zur Produktion von S-Adenosyl-L-Methionin durch Fermentation von genetisch modifizierten Mikroorganismen

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WO2004005450A2 WO2004005450A2 (de) 2004-01-15
WO2004005450A3 true WO2004005450A3 (de) 2004-04-15

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AU (1) AU2003246362A1 (de)
DE (1) DE10230224A1 (de)
WO (1) WO2004005450A2 (de)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105483190A (zh) * 2015-10-23 2016-04-13 山东金城生物药业有限公司 酿酒酵母基因工程改造提高s-腺苷-l-蛋氨酸产量的方法
CN106893726A (zh) * 2017-02-20 2017-06-27 天津大学 一种启动子以及重组酵母菌株

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL1731596T3 (pl) 2005-06-09 2013-08-30 Gnosis Spa Suszone, liofilizowane i/lub mikrokapsułkowane komórki Saccharomyces cerevisiae o dużej zawartości (S)-(+)-S-adenozylo-L-metioniny
CN105483153B (zh) * 2015-10-23 2019-09-20 山东金城生物药业有限公司 酿酒酵母代谢工程改造提高s-腺苷-l-蛋氨酸生产水平的方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5100786A (en) * 1987-12-28 1992-03-31 Kanegafuchi Kagaku Kogyo Kabushiki Kaisha Gene capable of enhancing S-adenosyl-L-methionine accumulation and process for producing S-adenosyl-L-methionine using the same
EP0647712A1 (de) * 1993-10-07 1995-04-12 Boehringer Ingelheim Espana S.A. Herstellung von S-Adenosyl-Methionin(SAM) durch Fermentation von transformierten Bakterien

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2139034T3 (es) * 1993-05-25 2000-02-01 American Cyanamid Co Seleccion de citocrome p-450 reductasa con respecto a inhibidores de la biosintesis del ergosterol.
US20020018774A1 (en) * 1998-12-17 2002-02-14 Simon Green CANDIDA ALBICANS mRNA 5'5-TRIPHOSPHATASE (CET-1) POLYNUCLEOTIDES
FR2801902B1 (fr) * 1999-12-01 2005-03-04 Centre Nat Rech Scient Procedes de criblages fonctionnels d'agents aptes a moduler l'activite des complexes ubiquitine-ligases scf et leurs applications

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5100786A (en) * 1987-12-28 1992-03-31 Kanegafuchi Kagaku Kogyo Kabushiki Kaisha Gene capable of enhancing S-adenosyl-L-methionine accumulation and process for producing S-adenosyl-L-methionine using the same
EP0647712A1 (de) * 1993-10-07 1995-04-12 Boehringer Ingelheim Espana S.A. Herstellung von S-Adenosyl-Methionin(SAM) durch Fermentation von transformierten Bakterien

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
DATABASE WPI Section Ch Week 199507, Derwent World Patents Index; Class B04, AN 1995-049854, XP002257478 *
ISNARD ANNE-DOMINIQUE ET AL: "The study of methionine uptake in Saccharomyces cerevisiae reveals a new family of amino acid permeases", JOURNAL OF MOLECULAR BIOLOGY, vol. 262, no. 4, 1996, pages 473 - 484, XP002257040, ISSN: 0022-2836 *
KODAMA YUKIKO ET AL: "Genome-wide expression analysis of genes affected by amino acid sensor Ssy1p in Saccharomyces cerevisiae", CURRENT GENETICS, vol. 41, no. 2, May 2002 (2002-05-01), pages 63 - 72, XP002257038, ISSN: 0172-8083 *
KOSUGI AKIHIKO ET AL: "MUP1, high affinity methionine permease, is involved in cysteine uptake by Saccharomyces cerevisiae", BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, vol. 65, no. 3, March 2001 (2001-03-01), pages 728 - 731, XP002257039, ISSN: 0916-8451 *
LEE K ET AL: "REGULATION OF SULFUR METABOLISM IN CEPHALOSPORIUM-ACREMONIUM", KOREAN JOURNAL OF APPLIED MICROBIOLOGY AND BIOENGINEERING, vol. 15, no. 5, 1987, pages 361 - 367, XP008023153, ISSN: 0257-2389 *
SHIOMI N ET AL: "PRODUCTION OF S-ADENOSYL-L-METHIONINE BY SACCHAROMYCES CEREVISIAE CELLS CARRYING A GENE FOR ETHIONINE RESISTANCE", BIOTECHNOLOGY AND BIOENGINEERING, INTERSCIENCE PUBLISHERS, LONDON, GB, vol. 35, no. 11, 1990, pages 1120 - 1124, XP008022498, ISSN: 0006-3592 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105483190A (zh) * 2015-10-23 2016-04-13 山东金城生物药业有限公司 酿酒酵母基因工程改造提高s-腺苷-l-蛋氨酸产量的方法
CN105483190B (zh) * 2015-10-23 2019-08-13 山东金城生物药业有限公司 酿酒酵母基因工程改造提高s-腺苷-l-蛋氨酸产量的方法
CN106893726A (zh) * 2017-02-20 2017-06-27 天津大学 一种启动子以及重组酵母菌株

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Publication number Publication date
AU2003246362A8 (en) 2004-01-23
WO2004005450A2 (de) 2004-01-15
AU2003246362A1 (en) 2004-01-23
DE10230224A1 (de) 2004-04-01

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