WO2004002216A3 - Harvest-inducible genes from alfalfa (medicago sativa) and methods of use thereof - Google Patents

Harvest-inducible genes from alfalfa (medicago sativa) and methods of use thereof Download PDF

Info

Publication number
WO2004002216A3
WO2004002216A3 PCT/CA2003/000964 CA0300964W WO2004002216A3 WO 2004002216 A3 WO2004002216 A3 WO 2004002216A3 CA 0300964 W CA0300964 W CA 0300964W WO 2004002216 A3 WO2004002216 A3 WO 2004002216A3
Authority
WO
WIPO (PCT)
Prior art keywords
alfalfa
harvest
methods
medicago sativa
genes
Prior art date
Application number
PCT/CA2003/000964
Other languages
French (fr)
Other versions
WO2004002216A2 (en
Inventor
Larry Erickson
Jian Zhang
Original Assignee
Univ Guelph
Larry Erickson
Jian Zhang
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Guelph, Larry Erickson, Jian Zhang filed Critical Univ Guelph
Priority to US10/519,811 priority Critical patent/US7388091B2/en
Priority to AU2003245772A priority patent/AU2003245772A1/en
Priority to EP03737803A priority patent/EP1517605A2/en
Priority to CA002490781A priority patent/CA2490781A1/en
Priority to US10/745,721 priority patent/US7329798B2/en
Publication of WO2004002216A2 publication Critical patent/WO2004002216A2/en
Publication of WO2004002216A3 publication Critical patent/WO2004002216A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The present invention relates to novel inducible genes from alfalfa. These genes are highly induced following mechanical harvesting or wounding of alfalfa. The genes are useful for inducible production of heterologous proteins following harvesting.
PCT/CA2003/000964 2002-06-28 2003-06-27 Harvest-inducible genes from alfalfa (medicago sativa) and methods of use thereof WO2004002216A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
US10/519,811 US7388091B2 (en) 2002-06-28 2003-06-27 Harvest-inducible genes from alfalfa (Medicago sativa) and methods of use thereof
AU2003245772A AU2003245772A1 (en) 2002-06-28 2003-06-27 Harvest-inducible genes form alfalfa (medicago sativa) and methods of use thereof
EP03737803A EP1517605A2 (en) 2002-06-28 2003-06-27 Harvest-inducible genes from alfalfa (medicago sativa) and methods of use thereof
CA002490781A CA2490781A1 (en) 2002-06-28 2003-06-27 Harvest-inducible genes from alfalfa (medicago sativa) and methods of use thereof
US10/745,721 US7329798B2 (en) 2002-06-28 2003-12-23 Harvest-inducible regulatory elements and methods of using same

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US39244402P 2002-06-28 2002-06-28
US60/392,444 2002-06-28

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US10/745,721 Continuation-In-Part US7329798B2 (en) 2002-06-28 2003-12-23 Harvest-inducible regulatory elements and methods of using same

Publications (2)

Publication Number Publication Date
WO2004002216A2 WO2004002216A2 (en) 2004-01-08
WO2004002216A3 true WO2004002216A3 (en) 2004-07-01

Family

ID=30000871

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA2003/000964 WO2004002216A2 (en) 2002-06-28 2003-06-27 Harvest-inducible genes from alfalfa (medicago sativa) and methods of use thereof

Country Status (5)

Country Link
US (1) US7388091B2 (en)
EP (1) EP1517605A2 (en)
AU (1) AU2003245772A1 (en)
CA (1) CA2490781A1 (en)
WO (1) WO2004002216A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7329798B2 (en) * 2002-06-28 2008-02-12 University Of Guelph Harvest-inducible regulatory elements and methods of using same
DE102004057291C5 (en) * 2004-11-26 2010-08-26 Südzucker AG Mannheim/Ochsenfurt Storage-induced promoters
CN115399286B (en) * 2022-07-21 2024-05-28 广西壮族自治区畜牧研究所 Livestock breeding method using alfalfa meal as feed

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001073090A2 (en) * 2000-03-24 2001-10-04 The Samuel Roberts Noble Foundation, Inc. Method for modifying lignin composition and increasing in vivo digestibility of forages

Family Cites Families (52)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL8300698A (en) 1983-02-24 1984-09-17 Univ Leiden METHOD FOR BUILDING FOREIGN DNA INTO THE NAME OF DIABIC LOBAL PLANTS; AGROBACTERIUM TUMEFACIENS BACTERIA AND METHOD FOR PRODUCTION THEREOF; PLANTS AND PLANT CELLS WITH CHANGED GENETIC PROPERTIES; PROCESS FOR PREPARING CHEMICAL AND / OR PHARMACEUTICAL PRODUCTS.
NL8300699A (en) 1983-02-24 1984-09-17 Univ Leiden METHOD FOR BUILDING FOREIGN DNA INTO THE NAME OF DIABIC LOBAL PLANTS; METHOD FOR PRODUCING AGROBACTERIUM TUMEFACIENS BACTERIEN; STABLE COINTEGRATE PLASMIDS; PLANTS AND PLANT CELLS WITH CHANGED GENETIC PROPERTIES; PROCESS FOR PREPARING CHEMICAL AND / OR PHARMACEUTICAL PRODUCTS.
US5504200A (en) 1983-04-15 1996-04-02 Mycogen Plant Science, Inc. Plant gene expression
DE3321346C2 (en) 1983-06-13 1987-04-09 Philips Patentverwaltung Gmbh, 2000 Hamburg Optical print head
JPS6083584A (en) 1983-10-13 1985-05-11 Rikagaku Kenkyusho Method for ingression of substance into live cell
US4784949A (en) 1984-04-19 1988-11-15 Cetus Corporation Universal dominant selectable marker cassette
US5231019A (en) 1984-05-11 1993-07-27 Ciba-Geigy Corporation Transformation of hereditary material of plants
US5149645A (en) 1984-06-04 1992-09-22 Rijksuniversiteit Leiden Process for introducing foreign DNA into the genome of plants
US4743548A (en) 1984-09-25 1988-05-10 Calgene, Inc. Plant cell microinjection technique
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
CA1340766C (en) 1984-12-24 1999-09-28 Clive Waldron Selectable marker for development of vectors and transformation systems in plants
US4943674A (en) 1987-05-26 1990-07-24 Calgene, Inc. Fruit specific transcriptional factors
US4940835A (en) 1985-10-29 1990-07-10 Monsanto Company Glyphosate-resistant plants
ES2018274T5 (en) 1986-03-11 1996-12-16 Plant Genetic Systems Nv VEGETABLE CELLS RESISTANT TO GLUTAMINE SYNTHETASE INHIBITORS, PREPARED BY GENETIC ENGINEERING.
US5120657A (en) 1986-12-05 1992-06-09 Agracetus, Inc. Apparatus for genetic transformation
AU2811889A (en) 1987-10-20 1989-05-23 Plant Genetic Systems N.V. A process for the production of biologically active peptide via the expression of modified storage seed protein genes in transgenic plants
US5179022A (en) 1988-02-29 1993-01-12 E. I. Du Pont De Nemours & Co. Biolistic apparatus for delivering substances into cells and tissues in a non-lethal manner
US5614395A (en) 1988-03-08 1997-03-25 Ciba-Geigy Corporation Chemically regulatable and anti-pathogenic DNA sequences and uses thereof
WO1990002484A1 (en) 1988-09-06 1990-03-22 Washington University Oral immunization by transgenic plants
DE3843627A1 (en) 1988-12-21 1990-07-05 Inst Genbiologische Forschung POTATO TUBE-SPECIFIC TRANSCRIPTIONAL REGULATION
US5723757A (en) 1988-12-21 1998-03-03 Hoechst Schering Agrevo Gmbh Plant promoters specific for sink organ expression of genes
US5302523A (en) 1989-06-21 1994-04-12 Zeneca Limited Transformation of plant cells
NL8901932A (en) 1989-07-26 1991-02-18 Mogen Int PRODUCTION OF heterologous PROTEINS IN PLANTS OR PLANTS.
US5202422A (en) 1989-10-27 1993-04-13 The Scripps Research Institute Compositions containing plant-produced glycopolypeptide multimers, multimeric proteins and method of their use
US5959177A (en) 1989-10-27 1999-09-28 The Scripps Research Institute Transgenic plants expressing assembled secretory antibodies
EP0472712B1 (en) 1990-03-16 2001-09-12 Calgene LLC Novel sequences preferentially expressed in early seed development and methods related thereto
US5543576A (en) 1990-03-23 1996-08-06 Mogen International Production of enzymes in seeds and their use
AU8914291A (en) 1990-11-23 1992-06-25 Plant Genetic Systems N.V. Process for transforming monocotyledonous plants
US5948682A (en) 1991-02-22 1999-09-07 Sembiosys Genetics Inc. Preparation of heterologous proteins on oil bodies
IL101508A0 (en) 1991-04-08 1992-12-30 Rhone Poulenc Agrochimie Chimeric plant genes based on upstream regulatory elements of helianthinin
US6034298A (en) 1991-08-26 2000-03-07 Prodigene, Inc. Vaccines expressed in plants
US5612487A (en) 1991-08-26 1997-03-18 Edible Vaccines, Inc. Anti-viral vaccines expressed in plants
US5484719A (en) 1991-08-26 1996-01-16 Edible Vaccines, Inc. Vaccines produced and administered through edible plants
EP0633940B1 (en) 1992-04-02 2002-12-04 SemBioSys Genetics Inc. Oil-body protein cis-elements as regulatory signals
EP0604662B1 (en) 1992-07-07 2008-06-18 Japan Tobacco Inc. Method of transforming monocotyledon
US5670349A (en) 1993-08-02 1997-09-23 Virginia Tech Intellectual Properties, Inc. HMG2 promoter expression system and post-harvest production of gene products in plants and plant cell cultures
ATE231733T1 (en) 1994-01-21 2003-02-15 Powderject Vaccines Inc GAS ACTUATED ELEMENT FOR DISCHARGING GENETIC MATERIAL
US6046037A (en) 1994-12-30 2000-04-04 Hiatt; Andrew C. Method for producing immunoglobulins containing protection proteins in plants and their use
US5565340A (en) 1995-01-27 1996-10-15 Clontech Laboratories, Inc. Method for suppressing DNA fragment amplification during PCR
FR2736285B1 (en) 1995-07-07 1997-08-22 Valois DEVICE FOR DISPENSING FLUID PRODUCT WITH BACTERIOSTATIC ACTION
US5859327A (en) 1995-08-22 1999-01-12 Genetronics, Inc. Electroporation-mediated molecular transfer in intact plants
WO1997010353A1 (en) 1995-09-14 1997-03-20 Virginia Tech Intellectual Property, Inc. Production of lysosomal enzymes in plant-based expression systems
US5804694A (en) 1995-11-06 1998-09-08 Prodigene, Inc. Commercial production of β-glucuronidase in plants
US5767379A (en) 1995-11-06 1998-06-16 John Howard Commercial production of avidin in plants
US5824870A (en) 1995-11-06 1998-10-20 Baszczynski; Chris Commercial production of aprotinin in plants
US5693512A (en) 1996-03-01 1997-12-02 The Ohio State Research Foundation Method for transforming plant tissue by sonication
US5981835A (en) 1996-10-17 1999-11-09 Wisconsin Alumni Research Foundation Transgenic plants as an alternative source of lignocellulosic-degrading enzymes
CA2221843A1 (en) 1998-01-27 1999-07-27 Her Majesty The Queen, In Right Of Canada, As Represented By The Ministe Ture Porcine reproductive and respiratory syndrome oral vaccine production in plants
AU4355099A (en) 1998-06-22 2000-01-10 Her Majesty The Queen In Right Of Canada As Represented By The Department Of Agriculture And Agri-Food Canada Non-food crop plant bioreactor
WO2000031251A1 (en) 1998-11-26 2000-06-02 University Of Otago Control of post harvest gene expression in plants by the use of harvest-regulated gene promoters
JP3427036B2 (en) 2000-03-30 2003-07-14 富士通日立プラズマディスプレイ株式会社 Display panel driving method and panel display device
US6783394B1 (en) 2003-03-18 2004-08-31 Randall A. Holliday Universal multi-stage compression connector

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001073090A2 (en) * 2000-03-24 2001-10-04 The Samuel Roberts Noble Foundation, Inc. Method for modifying lignin composition and increasing in vivo digestibility of forages

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
FERULLO JEAN-MARC ET AL: "Post-harvest alteration of in vitro translatable mRNA population in alfalfa (Medicago sativa L.)", CROP SCIENCE, CROP SCIENCE SOCIETY OF AMERICA, MADISON, WI, US, vol. 36, no. 4, 1996, pages 1011 - 1016, XP002146910, ISSN: 0011-183X *
KUHN EKKEHARD: "From library screening to microarray technology: Strategies to determine gene expression profiles and to identify differentially regulated genes in plants", ANNALS OF BOTANY (LONDON), vol. 87, no. 2, February 2001 (2001-02-01), pages 139 - 155, XP002259520, ISSN: 0305-7364 *
MATZ M V ET AL: "Different strategies of differential display: Areas of application", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 26, no. 24, 15 December 1998 (1998-12-15), pages 5537 - 5543, XP002245467, ISSN: 0305-1048 *

Also Published As

Publication number Publication date
CA2490781A1 (en) 2004-01-08
US7388091B2 (en) 2008-06-17
WO2004002216A2 (en) 2004-01-08
EP1517605A2 (en) 2005-03-30
AU2003245772A1 (en) 2004-01-19
US20060168676A1 (en) 2006-07-27

Similar Documents

Publication Publication Date Title
WO2001047952A3 (en) Insecticidal proteins from bacillus thuringiensis
WO2002057664A3 (en) Bacillus thuringiensis insecticidal proteins
ATE476427T1 (en) DIFLUOROMETHYL THIAZOLYL CARBOXANILIDE
WO2002088085A3 (en) Methods and intermediates for preparing 4-aminoquinoline cetp inhibitors
WO2005047511A3 (en) Bacterial expression of protease inhibitors and variants thereof
WO2004033659A3 (en) Novel tumor suppressor gene and compositions and methods for making and using the same
WO1997022619A3 (en) INHIBITORS OF INTERLEUKIN-1β CONVERTING ENZYME
WO2004003147A3 (en) Cngh0004 polypeptides, antibodies, compositions, methods and uses
WO2003087313A3 (en) Enhanced silk exsertion under stress
AU2002336768A1 (en) Identification of modulatory molecules using inducible promoters
WO2002038588A3 (en) Putrescine-n-methyltransferase promoter
WO2003010286A3 (en) Nucleic acids, polypeptides, and methods for modulating apoptosis
WO2003072602A3 (en) Protein complexes and methods for their use
WO2004002216A3 (en) Harvest-inducible genes from alfalfa (medicago sativa) and methods of use thereof
ATE446364T1 (en) ROOT-SPECIFIC STIMULANT-INDUCABLE PROMOTER AND USE THEREOF
NO20042429L (en) Novel immunogenic mimics of multimeric proteins
WO2005049814A3 (en) Novel atypical pneumonia-causing virus
PL372933A1 (en) Methylthiophene carboxanilides
WO2002053586A3 (en) Inhibitor of apoptosis proteins and nucleic acids and methods for making and using them
EP1338608A3 (en) Protein complexes and methods for their use
WO2001087931A3 (en) Bacterial insecticidal proteins
WO2003082912A3 (en) Transgenic plants expressing mussel adhesive proteins
WO2001012799A3 (en) Regulatory sequences for pollen specific or pollen abundant gene expression in plants
WO2007007327A3 (en) Il-32 modulators
WO2004033694A3 (en) Promoter to il-18bp, its preparation and use

Legal Events

Date Code Title Description
WWE Wipo information: entry into national phase

Ref document number: 10745721

Country of ref document: US

AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WWE Wipo information: entry into national phase

Ref document number: 2003245772

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 2003737803

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2490781

Country of ref document: CA

WWP Wipo information: published in national office

Ref document number: 2003737803

Country of ref document: EP

ENP Entry into the national phase

Ref document number: 2006168676

Country of ref document: US

Kind code of ref document: A1

WWE Wipo information: entry into national phase

Ref document number: 10519811

Country of ref document: US

NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Country of ref document: JP

WWP Wipo information: published in national office

Ref document number: 10519811

Country of ref document: US