WO2001012799A3 - Regulatory sequences for pollen specific or pollen abundant gene expression in plants - Google Patents

Regulatory sequences for pollen specific or pollen abundant gene expression in plants Download PDF

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Publication number
WO2001012799A3
WO2001012799A3 PCT/EP2000/008002 EP0008002W WO0112799A3 WO 2001012799 A3 WO2001012799 A3 WO 2001012799A3 EP 0008002 W EP0008002 W EP 0008002W WO 0112799 A3 WO0112799 A3 WO 0112799A3
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WO
WIPO (PCT)
Prior art keywords
pollen
plants
specific
gene expression
regulatory sequences
Prior art date
Application number
PCT/EP2000/008002
Other languages
French (fr)
Other versions
WO2001012799A2 (en
Inventor
Horst Loerz
Thomas Dresselhaus
Daniela Schreiber
Sigrid Heuer
Original Assignee
Horst Loerz
Thomas Dresselhaus
Daniela Schreiber
Sigrid Heuer
Sued Westdeutsche Saatzucht
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Horst Loerz, Thomas Dresselhaus, Daniela Schreiber, Sigrid Heuer, Sued Westdeutsche Saatzucht filed Critical Horst Loerz
Priority to AU67021/00A priority Critical patent/AU6702100A/en
Publication of WO2001012799A2 publication Critical patent/WO2001012799A2/en
Publication of WO2001012799A3 publication Critical patent/WO2001012799A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8238—Externally regulated expression systems chemically inducible, e.g. tetracycline
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8231—Male-specific, e.g. anther, tapetum, pollen
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Botany (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to regulatory elements for tissue specific, in particular pollen specific, expression of genes of interest in plants. The invention also relates to methods to obtain transgenic plants which show pollen specific expression of genes of interest and to methods for isolating pollen specific genes and/or pollen specific regulatory elements.
PCT/EP2000/008002 1999-08-18 2000-08-16 Regulatory sequences for pollen specific or pollen abundant gene expression in plants WO2001012799A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU67021/00A AU6702100A (en) 1999-08-18 2000-08-16 Regulatory sequences for pollen specific or pollen abundant gene expression in plants

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP99116268.6 1999-08-18
EP99116268 1999-08-18

Publications (2)

Publication Number Publication Date
WO2001012799A2 WO2001012799A2 (en) 2001-02-22
WO2001012799A3 true WO2001012799A3 (en) 2001-08-23

Family

ID=8238811

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/008002 WO2001012799A2 (en) 1999-08-18 2000-08-16 Regulatory sequences for pollen specific or pollen abundant gene expression in plants

Country Status (2)

Country Link
AU (1) AU6702100A (en)
WO (1) WO2001012799A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8022274B2 (en) 1998-09-22 2011-09-20 Mendel Biotechnology, Inc. Plant tolerance to low water, low nitrogen and cold
EP1325140A2 (en) * 2000-10-05 2003-07-09 Pioneer Hi-Bred International, Inc. A nitrate-responsive plant root specific transcriptional factor
US7309816B1 (en) 2004-08-04 2007-12-18 Pioneer Hi-Bred International, Inc. Zinc finger proteins expressed in plant meristem
CN111593134B (en) * 2019-02-21 2022-08-26 中国检验检疫科学研究院 Method, composition and kit for identifying four bee pollen by multiple fluorescence PCR

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5086169A (en) * 1989-04-20 1992-02-04 The Research Foundation Of State University Of New York Isolated pollen-specific promoter of corn
WO1994001572A2 (en) * 1992-07-09 1994-01-20 Pioneer Hi-Bred International, Inc. Maize pollen-specific polygalacturonase gene
AU3901395A (en) * 1994-11-22 1996-05-30 Commonwealth Scientific And Industrial Research Organisation Engineered sterile plants and uses therefor
WO1997030162A1 (en) * 1996-02-19 1997-08-21 Forbio Research Pty. Ltd. Regulation of gene expression in eukaryotes
WO1998013503A1 (en) * 1996-09-23 1998-04-02 F.B. Investments Pty. Ltd. A plant and method of modification
US5744693A (en) * 1992-10-02 1998-04-28 California Institute Of Technology Plants having altered floral development
CA2227940A1 (en) * 1998-04-06 1999-10-06 Steven H. Strauss Floral homeotic genes for manipulation of flowering in poplar trees and other plant species
EP1033405A2 (en) * 1999-02-25 2000-09-06 Ceres Incorporated Sequence-determined DNA fragments and corresponding polypeptides encoded thereby
WO2000055172A1 (en) * 1999-03-17 2000-09-21 Carter Holt Harvey Limited Plants having modified reproductive capacity

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5086169A (en) * 1989-04-20 1992-02-04 The Research Foundation Of State University Of New York Isolated pollen-specific promoter of corn
WO1994001572A2 (en) * 1992-07-09 1994-01-20 Pioneer Hi-Bred International, Inc. Maize pollen-specific polygalacturonase gene
US5744693A (en) * 1992-10-02 1998-04-28 California Institute Of Technology Plants having altered floral development
AU3901395A (en) * 1994-11-22 1996-05-30 Commonwealth Scientific And Industrial Research Organisation Engineered sterile plants and uses therefor
WO1997030162A1 (en) * 1996-02-19 1997-08-21 Forbio Research Pty. Ltd. Regulation of gene expression in eukaryotes
WO1998013503A1 (en) * 1996-09-23 1998-04-02 F.B. Investments Pty. Ltd. A plant and method of modification
CA2227940A1 (en) * 1998-04-06 1999-10-06 Steven H. Strauss Floral homeotic genes for manipulation of flowering in poplar trees and other plant species
EP1033405A2 (en) * 1999-02-25 2000-09-06 Ceres Incorporated Sequence-determined DNA fragments and corresponding polypeptides encoded thereby
WO2000055172A1 (en) * 1999-03-17 2000-09-21 Carter Holt Harvey Limited Plants having modified reproductive capacity

Non-Patent Citations (11)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL 15 February 2000 (2000-02-15), WALBOT, V.: "660039F01.x1 660 - Mixed stages of anther and pollen Zea mays cDNA, mRNA sequence.", XP002161928 *
DATABASE EMBL 18 October 1999 (1999-10-18), WALBOT, V.: "614044G10.y1 614 - root cDNA library from Walbot Lab Zea mays cDNA, mRNA sequence", XP002161927 *
DATABASE EMBL 21 February 2000 (2000-02-21), WALBOT, V., ET AL.: "660039F01.y1 660 - Mixed stages of anther and pollen Zea mays cDNA, mRNA sequence.", XP002161926 *
DATABASE EMBL 23 September 1999 (1999-09-23), WALBOT, V., ET AL.: "614073H05.x1 614 - root cDNA library from Walbot Lab Zea mays cDNA, mRNA sequence.", XP002161925 *
DATABASE EMBL 24 September 1998 (1998-09-24), OZERSKY, P., ET AL.: "Arabidopsis thaliana BAC T25C13", XP002161923 *
DATABASE EMBL 25 March 1999 (1999-03-25), BEVAN, M., ET AL.: "Arabidopsis thaliana DNA chormosome 4, BAC clone T4F9 (ESSA project)", XP002161924 *
DATABASE WPI Section Ch Week 199629, Derwent World Patents Index; Class C06, AN 1996-278411, XP002161929 *
DATABASE WPI Section Ch Week 200010, Derwent World Patents Index; Class C06, AN 2000-106662, XP002161930 *
HEUER SIGRID ET AL: "The MADS box gene ZmMADS2 is specifically expressed in maize pollen and during maize pollen tube growth.", SEXUAL PLANT REPRODUCTION, vol. 13, no. 1, July 2000 (2000-07-01), pages 21 - 27, XP000982694, ISSN: 0934-0882 *
THEISSEN GUENTER ET AL: "Structural characterization, chromosomal localization and phylogenetic evaluation of two pairs of AGAMOUS-like MADS-box genes from maize.", GENE (AMSTERDAM), vol. 156, no. 2, 1995, pages 155 - 166, XP002161922, ISSN: 0378-1119 *
ZACHGO SABINE ET AL: "Pollen-specific expression of DEFH125, a MADS-box transcription factor in Antirrhinum with unusual features.", PLANT JOURNAL, vol. 11, no. 5, 1997, pages 1043 - 1050, XP002161921, ISSN: 0960-7412 *

Also Published As

Publication number Publication date
AU6702100A (en) 2001-03-13
WO2001012799A2 (en) 2001-02-22

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