WO2003100420A3 - Elektrochemischer enzymimmunoassay zur simultanen erfassung mehrerer analyten - Google Patents

Elektrochemischer enzymimmunoassay zur simultanen erfassung mehrerer analyten Download PDF

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Publication number
WO2003100420A3
WO2003100420A3 PCT/EP2003/005623 EP0305623W WO03100420A3 WO 2003100420 A3 WO2003100420 A3 WO 2003100420A3 EP 0305623 W EP0305623 W EP 0305623W WO 03100420 A3 WO03100420 A3 WO 03100420A3
Authority
WO
WIPO (PCT)
Prior art keywords
analytes
different
enzyme immunoassay
simultaneously detecting
electrochemical enzyme
Prior art date
Application number
PCT/EP2003/005623
Other languages
English (en)
French (fr)
Other versions
WO2003100420A2 (de
Inventor
Jae-Chul Pyun
Sang-Doo Kim
Ji-Won Chung
Original Assignee
Kist Europe Forschungsges Mbh
Jae-Chul Pyun
Sang-Doo Kim
Ji-Won Chung
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kist Europe Forschungsges Mbh, Jae-Chul Pyun, Sang-Doo Kim, Ji-Won Chung filed Critical Kist Europe Forschungsges Mbh
Priority to AU2003237711A priority Critical patent/AU2003237711A1/en
Publication of WO2003100420A2 publication Critical patent/WO2003100420A2/de
Publication of WO2003100420A3 publication Critical patent/WO2003100420A3/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Die vorliegende Erfindung betrifft ein Verfahren zur Durchführung eines Enzymimmunoassays (EIA, ELISA) zur qualitativen und/oder quantitativen Erfassung von Analyten in einer Probe. Dabei wird die Probe und ei­ne der Anzahl der zu bestimmenden Analyten entspre­chende Anzahl Antikörpertypen, die mit einer der An­zahl der zu bestimmenden Analyten entsprechenden An­zahl verschiedener Enzyme gekoppelt sind, in ein ge­meinsames Reaktionsgefäss gegeben. Zur Erkennung der unterschiedlichen Analyten ist jeweils ein Antikörper spezifisch für jeweils einen der Analyten, und je­weils für verschiedene Analyten spezifische Antikör­per sind mit jeweils verschiedenen Enzymen gekoppelt. Durch die verschiedenen Enzyme sind jeweils verschie­dene Substrate umsetzbar und/oder jeweils verschiede­ne Produkte erzeugbar.
PCT/EP2003/005623 2002-05-29 2003-05-28 Elektrochemischer enzymimmunoassay zur simultanen erfassung mehrerer analyten WO2003100420A2 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003237711A AU2003237711A1 (en) 2002-05-29 2003-05-28 Electrochemical enzyme immunoassay for simultaneously detecting a number of analytes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10223960.6 2002-05-29
DE2002123960 DE10223960A1 (de) 2002-05-29 2002-05-29 Enzymimmunoassay

Publications (2)

Publication Number Publication Date
WO2003100420A2 WO2003100420A2 (de) 2003-12-04
WO2003100420A3 true WO2003100420A3 (de) 2004-04-01

Family

ID=29432442

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2003/005623 WO2003100420A2 (de) 2002-05-29 2003-05-28 Elektrochemischer enzymimmunoassay zur simultanen erfassung mehrerer analyten

Country Status (3)

Country Link
AU (1) AU2003237711A1 (de)
DE (1) DE10223960A1 (de)
WO (1) WO2003100420A2 (de)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2940318B1 (fr) * 2008-12-22 2011-05-13 Centre Nat Rech Scient Nouveaux biotracteurs et leurs utilisations pour le controle des installations de filtration
CN110082347B (zh) * 2019-05-30 2021-12-24 中国科学院成都生物研究所 一种简易的尿糖定量检测方法以及尿糖定量检测试剂盒

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4914023A (en) * 1986-06-10 1990-04-03 Serono Diagnostics Partners Method of extending the linear range of an enzyme immunoassay by measuring off-peak absorbance
WO1995001155A1 (en) * 1993-07-01 1995-01-12 Unilever N.V. Oral compositions

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4914023A (en) * 1986-06-10 1990-04-03 Serono Diagnostics Partners Method of extending the linear range of an enzyme immunoassay by measuring off-peak absorbance
WO1995001155A1 (en) * 1993-07-01 1995-01-12 Unilever N.V. Oral compositions

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DAN LI WANG ET AL: "MINIATURIZED FLEXIBLE AMPEROMETRIC LACTATE PROBE", ANALYTICAL CHEMISTRY, AMERICAN CHEMICAL SOCIETY. COLUMBUS, US, vol. 65, no. 8, 15 April 1993 (1993-04-15), pages 1069 - 1073, XP000368508, ISSN: 0003-2700 *
TATSUMA TETSU ET AL: "Enzyme monolayer- and bilayer-modified Tin oxide electrodes for the determination of hydrogen peroxide and glucose", ANAL CHEM;ANALYTICAL CHEMISTRY NOV 1 1989, vol. 61, no. 21, 1 November 1989 (1989-11-01), pages 2352 - 2355, XP002263990 *

Also Published As

Publication number Publication date
WO2003100420A2 (de) 2003-12-04
AU2003237711A1 (en) 2003-12-12
DE10223960A1 (de) 2003-12-11

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