WO2003099780A3 - Substrats luminogenes pour proteases - Google Patents

Substrats luminogenes pour proteases Download PDF

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Publication number
WO2003099780A3
WO2003099780A3 PCT/US2003/016530 US0316530W WO03099780A3 WO 2003099780 A3 WO2003099780 A3 WO 2003099780A3 US 0316530 W US0316530 W US 0316530W WO 03099780 A3 WO03099780 A3 WO 03099780A3
Authority
WO
WIPO (PCT)
Prior art keywords
protease
protease substrates
luminogenic
assays
compositions
Prior art date
Application number
PCT/US2003/016530
Other languages
English (en)
Other versions
WO2003099780A2 (fr
Inventor
Zhenjun Diwu
Jinfang Liao
Zhiqiang Wang
Original Assignee
Molecular Devices Corp
Zhenjun Diwu
Jinfang Liao
Zhiqiang Wang
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Molecular Devices Corp, Zhenjun Diwu, Jinfang Liao, Zhiqiang Wang filed Critical Molecular Devices Corp
Priority to EP03729139A priority Critical patent/EP1517997A4/fr
Priority to AU2003234638A priority patent/AU2003234638A1/en
Publication of WO2003099780A2 publication Critical patent/WO2003099780A2/fr
Publication of WO2003099780A3 publication Critical patent/WO2003099780A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/02—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing at least one abnormal peptide link
    • C07K5/021—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing at least one abnormal peptide link containing the structure -NH-(X)n-C(=0)-, n being 5 or 6; for n > 6, classification in C07K5/06 - C07K5/10, according to the moiety having normal peptide bonds
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
    • C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
    • C07D311/78—Ring systems having three or more relevant rings
    • C07D311/80—Dibenzopyrans; Hydrogenated dibenzopyrans
    • C07D311/82—Xanthenes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D493/00—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system
    • C07D493/02—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system in which the condensed system contains two hetero rings
    • C07D493/10—Spiro-condensed systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/02—Linear peptides containing at least one abnormal peptide link
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

L'invention concerne des systèmes, comprenant des compositions, des kits et des procédés adaptés à la réalisation des tests enzymatiques, en particulier de tests de protéases. La composition peut comprendre des dérivés de rhodamine 110, substitués sur les azotes de l'entité xanthylium respectivement par un groupe peptidique et un groupe morpholine-4-carbonyle. Ces compositions peuvent constituer des substrats luminogènes pour une grande variété de protéases, avec une utilité dans une variété de tests enzymatiques sensibles aux protéases.
PCT/US2003/016530 2002-05-24 2003-05-23 Substrats luminogenes pour proteases WO2003099780A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP03729139A EP1517997A4 (fr) 2002-05-24 2003-05-23 Substrats luminogenes pour proteases
AU2003234638A AU2003234638A1 (en) 2002-05-24 2003-05-23 Luminogenic protease substrates

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US38336302P 2002-05-24 2002-05-24
US60/383,363 2002-05-24

Publications (2)

Publication Number Publication Date
WO2003099780A2 WO2003099780A2 (fr) 2003-12-04
WO2003099780A3 true WO2003099780A3 (fr) 2004-04-01

Family

ID=29584553

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/016530 WO2003099780A2 (fr) 2002-05-24 2003-05-23 Substrats luminogenes pour proteases

Country Status (3)

Country Link
EP (1) EP1517997A4 (fr)
AU (1) AU2003234638A1 (fr)
WO (1) WO2003099780A2 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005047245A2 (fr) 2003-11-14 2005-05-26 Wisconsin Alumni Research Foundation Dosages par fluorescence à sensibilité améliorée
WO2009045104A1 (fr) * 2007-10-05 2009-04-09 Academisch Ziekenhuis Bij De Universiteit Van Amsterdam Procédé de diagnostic d'un rejet présymptomatique de greffe d'organes
US8460862B2 (en) * 2009-03-11 2013-06-11 Marker Gene Technologies Enzyme substrates for visualizing acidic organelles
WO2011087000A1 (fr) * 2010-01-13 2011-07-21 国立大学法人 東京大学 Diagnostic pour le cancer
FR2978163B1 (fr) * 2011-07-22 2013-08-30 Stago Diagnostica Utilisation d'un substrat dans une methode pour mesurer l'activite des enzymes proteolytiques disponibles
FR2978164B1 (fr) * 2011-07-22 2013-08-30 Stago Diagnostica Utilisation d'un substrat dans une methode pour mesurer l'activite des enzymes proteolytiques
EP2875040B1 (fr) 2012-07-19 2017-10-18 Kingston University Higher Education Corporation Sonde fluorescente à base de rhodamine pour l'activité coagulase et la détection de souches bactériennes produisant de la coagulase
JP6731669B2 (ja) 2014-07-11 2020-07-29 国立大学法人 東京大学 ジペプチジルペプチダーゼｉｖ検出用蛍光プローブ
EP3954697B1 (fr) * 2015-02-27 2024-01-03 The University of Tokyo Sonde fluorescente pour détecter l'activité de la calpaïne
CN109438319B (zh) * 2018-09-17 2022-05-10 湖北大学 一种检测亮氨酸氨肽酶的化合物及其制备方法和应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4557862A (en) * 1983-10-28 1985-12-10 University Patents, Inc. Rhodamine derivatives as fluorogenic substrates for proteinases
US4640893A (en) * 1983-10-28 1987-02-03 University Of Illinois Novel rhodamine derivatives as fluorogenic substrates for proteinases

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69920473T2 (de) * 1998-07-21 2006-02-23 Cytovia, Inc., San Diego Neue fluoreszenzfarbstoffe und ihre anwendung in fluoreszenznachweisverfahren ganzer zellen für kaspasen, peptidasen, proteasen und andere enzyme sowie deren verwendung
EP1090073B1 (fr) * 1999-04-23 2003-03-05 Molecular Probes Inc. Colorants a base de xanthene et leur utilisation en tant que composes d'extinction de luminescence

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4557862A (en) * 1983-10-28 1985-12-10 University Patents, Inc. Rhodamine derivatives as fluorogenic substrates for proteinases
US4640893A (en) * 1983-10-28 1987-02-03 University Of Illinois Novel rhodamine derivatives as fluorogenic substrates for proteinases

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP1517997A4 *

Also Published As

Publication number Publication date
AU2003234638A8 (en) 2003-12-12
EP1517997A4 (fr) 2006-11-15
EP1517997A2 (fr) 2005-03-30
WO2003099780A2 (fr) 2003-12-04
AU2003234638A1 (en) 2003-12-12

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