WO2003092360A3 - Novel glyphosate-n-acetyltransferase (gat) genes - Google Patents

Novel glyphosate-n-acetyltransferase (gat) genes Download PDF

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Publication number
WO2003092360A3
WO2003092360A3 PCT/US2003/013638 US0313638W WO03092360A3 WO 2003092360 A3 WO2003092360 A3 WO 2003092360A3 US 0313638 W US0313638 W US 0313638W WO 03092360 A3 WO03092360 A3 WO 03092360A3
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WO
WIPO (PCT)
Prior art keywords
novel
proteins
compounds
glyphosate
acetyltransferase
Prior art date
Application number
PCT/US2003/013638
Other languages
French (fr)
Other versions
WO2003092360A2 (en
Inventor
Linda A Castle
Dan Siehl
Lorraine J Giver
Jeremy Minshull
Christina Ivy
Yong Hong Chen
Phil Patten
Rebecca Gorton
Nicholas B Duck
Billy F Mccutchen
Roger Kemble
Original Assignee
Verdia Inc
Pioneer Hi Bred Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Verdia Inc, Pioneer Hi Bred Int filed Critical Verdia Inc
Priority to AU2003234328A priority Critical patent/AU2003234328A1/en
Publication of WO2003092360A2 publication Critical patent/WO2003092360A2/en
Publication of WO2003092360A3 publication Critical patent/WO2003092360A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Novel proteins are provided herein, including proteins capable of catalyzing the acetylation of glyphosate and other structurally related proteins. Also provided are novel polynucleotides capable of encoding these proteins, compositions that include one or more of these novel proteins and/or polynucleotides, recombinant cells and transgenic plants comprising these novel compounds, diversification methods involving the novel compounds, and methods of using the compounds. Some of the novel methods and compounds provided herein can be used to render an organism, such as a plant, resistant to glyphosate.
PCT/US2003/013638 2002-04-30 2003-04-30 Novel glyphosate-n-acetyltransferase (gat) genes WO2003092360A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003234328A AU2003234328A1 (en) 2002-04-30 2003-04-30 Novel glyphosate-n-acetyltransferase (gat) genes

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US37771902P 2002-04-30 2002-04-30
US60/377,719 2002-04-30
US37717502P 2002-05-01 2002-05-01
US60/377,175 2002-05-01

Publications (2)

Publication Number Publication Date
WO2003092360A2 WO2003092360A2 (en) 2003-11-13
WO2003092360A3 true WO2003092360A3 (en) 2005-03-17

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/013638 WO2003092360A2 (en) 2002-04-30 2003-04-30 Novel glyphosate-n-acetyltransferase (gat) genes

Country Status (4)

Country Link
AR (2) AR039501A1 (en)
AU (1) AU2003234328A1 (en)
CL (2) CL2009000280A1 (en)
WO (1) WO2003092360A2 (en)

Cited By (4)

* Cited by examiner, † Cited by third party
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US7803992B2 (en) 2005-08-24 2010-09-28 Pioneer Hi-Bred International, Inc. Methods and compositions for expressing an herbicide-tolerant polynucleotide
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US9574201B2 (en) 2010-06-09 2017-02-21 Bayer Cropscience Nv Methods and means to modify a plant genome at a nucleotide sequence commonly used in plant genome engineering
US9593317B2 (en) 2010-06-09 2017-03-14 Bayer Cropscience Nv Methods and means to modify a plant genome at a nucleotide sequence commonly used in plant genome engineering

Families Citing this family (249)

* Cited by examiner, † Cited by third party
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AU2009201716B2 (en) * 2000-10-30 2012-05-17 E. I. Du Pont De Nemours And Company Novel glyphosate-N-acetyltransferase (GAT) genes
US7462481B2 (en) 2000-10-30 2008-12-09 Verdia, Inc. Glyphosate N-acetyltransferase (GAT) genes
EP1620557A2 (en) * 2003-04-29 2006-02-01 Pioneer Hi-Bred International, Inc. Novel glyphosate-n-acetyltransferase (gat) genes
US7405074B2 (en) 2004-04-29 2008-07-29 Pioneer Hi-Bred International, Inc. Glyphosate-N-acetyltransferase (GAT) genes
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CN1313614C (en) * 2005-10-17 2007-05-02 中国农业科学院生物技术研究所 Glyphosate acetyl transferase gene and its application
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