WO2003090775A1 - Foods and drugs containing silkworm and wild silkworm silk powder for antidementia effect and improving memorization learning - Google Patents

Abstract

It is intended to clarify that silkworm and wild silkworm silk powders have an antidementia effect and an effect of improving memorization learning. Foods and drugs containing silkworm and wild silkworm silk powders are administered to mammals including humans.

Description

 P leak 2/09051

Description Foods and pharmaceuticals containing silk powder of silkworms and wild silkworms to improve anti-dementia effects and improve memory learning

 The present invention relates to a method for improving the anti-dementia effect and the memory learning effect of mammals including humans. Technology background

 Silkworm and silkworm silk powders are pulverized by various manufacturing techniques, and the amino acid composition of the low molecular weight pulverized protein is a known amino acid composition because it is a protein at the mouth of the fiber. It is used for foods and cosmetics as its usage.

 However, it has not yet been clarified that silk powder of silkworms and wild silkworms has an anti-dementia effect and an effect of improving memory and learning. Problems to be solved by the invention

 Therefore, since the amino acids constituting the protein of the fibular mouth such as glycine, alanine, serine, and tyrosine are abundant, anti-dementia effects and memory learning improvement effects are expected for silkworms and silkworm wild silkworms.

Means for solving the problem

 Silkworms and wild silkworm silk powder prepared according to known production methods (acid hydrolysis, enzymatic hydrolysis) were allowed to freely ingest mice (five-week-old ICR female) orally as an aqueous solution, and tap water was used as a control.ゥ Serum albumin (adopted as brush-po) Oral administration of aqueous solution. Disclosure of the invention

 The present invention is embodied in the form described above, and achieves the effects described below.

By administering foods and medicines containing silk powder from silkworms and wild silkworms to mammals including humans, an anti-dementia effect and a memory learning effect can be obtained. T JP02 / 09051

2 Brief description of drawings

 Fig. 1 This is the result of performing a step-through passive avoidance experiment for two days 30 days after the start of ingestion. BEST MODE FOR CARRYING OUT THE INVENTION

 Thirty days after the start of ingestion, a step-through passive avoidance experiment is performed for two days.

 For passive avoidance, mice are divided into three groups: water (n = 28), silk powder (n = 31), and bovine serum albumin (n = 22).

 On the first day of the experiment, place the mouse in the light room with the guillotine door closed 15 minutes after the pre-acquisition trial measurement. After 30 seconds open the door and measure the time to enter the darkroom

(Hereinafter, it will be referred to as an acquisition attempt), and upon entry, close the door and apply electrical stimulation (0.5 Ma, 4 seconds). The time to enter the darkroom is measured assuming that the mouse enters the darkroom when the hind legs of the mouse have completely entered. Twenty-four hours later (day 2 of the experiment), the mouse is placed in the light room with the door closed. After 30 seconds, open the door and measure the time it takes to enter the darkroom (hereinafter referred to as pre-dose trial). Mice with a pre-administration trial of less than 180 seconds are considered non-achievement mice and will not be subjected to subsequent regeneration trials.

 Next, scopolamine, which causes temporary dementia, is injected subcutaneously, and 15 minutes later, the mouse is placed in a light room with the door closed, and 30 seconds later, the time until the door is opened and a dark room is measured (hereinafter referred to as reproduction) Trial). In this experiment, data analysis is performed using nonparametric analysis. Tests within the same county use the Wilcoxon signed rank test, and tests between different groups use the Mann-Whitney U test to find significant differences. The example shown in FIG. 1 is the result of the memory ability (sec) in the acquisition trial, the scopol amine administration trial, and the regeneration trial. As a result, when 0.5 mg / kg body weight of scopol amine was administered to each mouse to cause temporary dementia, the response latency in the regeneration trial was increased between the silk powder group and the tap water and the serum albumin. A significant difference is observed. * Ku 0.01 and Ku 0.05 show significant differences between the other two groups in the silk powder group in the regeneration trial (Mann-Whitney U test). ## 0.01 indicates a significant difference between the pre-dose trials and the regeneration trials in the three groups (Wilcoxen's signed rank test).

 That is, oral administration of silk powder in a mouse behavioral experiment has the effect of suppressing temporary dementia.

Claims

Three

1. Silk powder of silkworms and wild silkworms is a fiproin protein composed mainly of repeating amino acids of glycine, alanine, serine, and tyrosine. It is made into low molecular powder by various manufacturing processes (free amino acids and oligopeptides). The product is based on a known amino acid composition. By utilizing this, we will develop applications in the food and medical fields to improve anti-dementia effects and improve memory and learning.

 2. It has not been clarified that silk parrots of silkworms and wild silkworms have anti-dementia effects and memory-learning effects. Therefore, the composition of the fibrillary in-proteins such as glycine, alanine, serine, and tyrosine is considered.

A method characterized by having an anti-dementia effect and improving memory and learning in silk powder of silkworms and wild silkworms due to abundance of amino acids.