WO2003089663A3 - Methodes de mesure d'activite enzymatique - Google Patents

Methodes de mesure d'activite enzymatique Download PDF

Info

Publication number
WO2003089663A3
WO2003089663A3 PCT/GB2003/001669 GB0301669W WO03089663A3 WO 2003089663 A3 WO2003089663 A3 WO 2003089663A3 GB 0301669 W GB0301669 W GB 0301669W WO 03089663 A3 WO03089663 A3 WO 03089663A3
Authority
WO
WIPO (PCT)
Prior art keywords
enzyme activity
measuring enzyme
methods
relates
measuring
Prior art date
Application number
PCT/GB2003/001669
Other languages
English (en)
Other versions
WO2003089663A2 (fr
Inventor
John Gerard Whateley
Original Assignee
Amersham Biosciences Uk Ltd
John Gerard Whateley
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amersham Biosciences Uk Ltd, John Gerard Whateley filed Critical Amersham Biosciences Uk Ltd
Priority to DE60336217T priority Critical patent/DE60336217D1/de
Priority to IL16434703A priority patent/IL164347A0/xx
Priority to JP2003586372A priority patent/JP4713835B2/ja
Priority to AU2003229914A priority patent/AU2003229914A1/en
Priority to AT03722751T priority patent/ATE500342T1/de
Priority to CA2484467A priority patent/CA2484467C/fr
Priority to EP03722751A priority patent/EP1497452B1/fr
Publication of WO2003089663A2 publication Critical patent/WO2003089663A2/fr
Publication of WO2003089663A3 publication Critical patent/WO2003089663A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/25—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving enzymes not classifiable in groups C12Q1/26 - C12Q1/66
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2337/00—N-linked chromogens for determinations of peptidases and proteinases
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/9015—Ligases (6)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/04—Screening involving studying the effect of compounds C directly on molecule A (e.g. C are potential ligands for a receptor A, or potential substrates for an enzyme A)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

L'invention concerne des méthodes de fluorescence pour mesurer l'activité enzymatique, en particulier les activités de clivage et d'assemblage enzymatiques. L'invention concerne également des substrats fluorogènes lesquels sont utiles pour mesurer l'activité enzymatique telle que dans des sondes d'imagerie in vitro et in vivo.
PCT/GB2003/001669 2002-04-19 2003-04-17 Methodes de mesure d'activite enzymatique WO2003089663A2 (fr)

Priority Applications (7)

Application Number Priority Date Filing Date Title
DE60336217T DE60336217D1 (de) 2002-04-19 2003-04-17 Verfahren zur bestimmung von enzym aktivität
IL16434703A IL164347A0 (en) 2002-04-19 2003-04-17 Methods for measuring enzyme activity
JP2003586372A JP4713835B2 (ja) 2002-04-19 2003-04-17 酵素活性測定方法
AU2003229914A AU2003229914A1 (en) 2002-04-19 2003-04-17 Methods for measuring enzyme activity
AT03722751T ATE500342T1 (de) 2002-04-19 2003-04-17 Verfahren zur bestimmung von enzym aktivität
CA2484467A CA2484467C (fr) 2002-04-19 2003-04-17 Methodes de mesure a base de fluorescence de l'activite enzymatique
EP03722751A EP1497452B1 (fr) 2002-04-19 2003-04-17 Methodes de mesure d'activite enzymatique

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0208989.4 2002-04-19
GBGB0208989.4A GB0208989D0 (en) 2002-04-19 2002-04-19 Methods for measuring enzyme activity

Publications (2)

Publication Number Publication Date
WO2003089663A2 WO2003089663A2 (fr) 2003-10-30
WO2003089663A3 true WO2003089663A3 (fr) 2004-05-27

Family

ID=9935152

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2003/001669 WO2003089663A2 (fr) 2002-04-19 2003-04-17 Methodes de mesure d'activite enzymatique

Country Status (9)

Country Link
US (2) US7727739B2 (fr)
EP (1) EP1497452B1 (fr)
JP (1) JP4713835B2 (fr)
AT (1) ATE500342T1 (fr)
AU (1) AU2003229914A1 (fr)
CA (1) CA2484467C (fr)
DE (1) DE60336217D1 (fr)
GB (2) GB0208989D0 (fr)
WO (1) WO2003089663A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101372632B1 (ko) 2011-08-26 2014-03-14 동국대학교 산학협력단 Ｘｐｇ 엔도뉴클레아제 활성 분석 방법

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0113434D0 (en) * 2001-06-04 2001-07-25 Amersham Pharm Biotech Uk Ltd Quinacridone derivatives as labels for fluorescence detection of biological materials
JP4637565B2 (ja) * 2004-11-02 2011-02-23 一般社団法人オンチップ・セロミクス・コンソーシアム 生体試料標識物および生体物質標識法および生体物質の検査法
US20090220587A1 (en) * 2005-02-01 2009-09-03 United State Army Liposomal drug delivery constructs targeted by lipid-conjugated peptide ligands
US7741431B2 (en) * 2005-02-01 2010-06-22 The United States Of America As Represented By The Secretary Of The Army Liposomes containing novel targeting and/or fusogenic peptides, preparations containing them and therapeutic use thereof
EP1900822B1 (fr) * 2005-05-24 2016-02-03 Suzuki, Miho Procédé d'analyse simultanée de réactions biologiques multiples ou de changements multiples en conditions in vivo
US9127164B2 (en) 2005-10-28 2015-09-08 Almac Sciences (Scotland) Limited Fluorescent dyes and uses thereof
US8691939B2 (en) 2007-06-08 2014-04-08 General Electric Company Compositions, methods, and kits for assaying complement activation
WO2009112791A1 (fr) * 2008-03-14 2009-09-17 Assaymetrics Limited Peptides fluorogènes et leur procédé de production
JP2010215572A (ja) * 2009-03-17 2010-09-30 Japan Health Science Foundation 歯髄炎診断マーカー及び歯髄炎診断システム
JP5697129B2 (ja) * 2010-03-24 2015-04-08 国立大学法人埼玉大学 Ｆｒｅｔを利用した酵素活性測定基質及びその製造方法
WO2012110833A2 (fr) 2011-02-18 2012-08-23 Almac Sciences (Scotland) Limited Modulateurs de fluorescence
US9180209B2 (en) 2011-02-28 2015-11-10 The Board Of Trustees Of The Leland Stanford Junior University Non-peptidic quenched fluorescent imaging probes
WO2012131345A2 (fr) 2011-03-25 2012-10-04 Almac Sciences (Scotland) Limited Essais enzymatiques
WO2012170727A1 (fr) * 2011-06-07 2012-12-13 Life Technologies Corporation Nanocristaux semi-conducteurs fluorogéniques
GB201122099D0 (en) 2011-12-22 2012-02-01 Almac Sciences Scotland Ltd Dyes
EP2841592B8 (fr) 2012-03-23 2017-05-03 Surmodics IVD, Inc. Compositions et procédés pour tests de diagnostic in vitro comportant de l'acide sulfonique
RU2622753C1 (ru) * 2016-11-17 2017-06-19 Федеральное государственное бюджетное учреждение "Национальный научно-практический центр сердечно-сосудистой хирургии имени А.Н. Бакулева" Министерства здравоохранения Российской Федерации (ФГБУ "ННПЦССХ им. А.Н. Бакулева" Минздрава России) Способ определения активности ангиотензин-превращающего фермента в тканях сердца
CN114605405B (zh) * 2022-03-09 2023-09-05 吉林大学 一种基于喹吖啶酮骨架的细胞脂滴荧光成像探针及其应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1979000602A1 (fr) * 1978-02-07 1979-08-23 Kabi Ab Substrats facilement dissociables pour la determination quantitative de proteases
WO2000073437A1 (fr) * 1999-05-27 2000-12-07 Merck Frosst Canada & Co. Analyses permettant de detecter l'activite de caspases a l'aide de proteines fluorescentes vertes
US20010005752A1 (en) * 1998-12-21 2001-06-28 Manfred Auer Fluorescent dyes (AIDA) for solid phase and solution phase screening

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE17262T1 (de) 1981-11-02 1986-01-15 Pentapharm Ag Verfahren zur quantitativen bestimmung von blutgerinnungsfaktor xii in humanplasma.
US4812409A (en) 1986-01-31 1989-03-14 Eastman Kodak Company Hydrolyzable fluorescent substrates and analytical determinations using same
FR2635114A1 (fr) * 1988-08-02 1990-02-09 Cibevial Sa Procede de traitement de solutions proteiques contenant des pigments tels que groupements heminiques ou chlorophylles en vue de leur decoloration et produits obtenus
EP0428000A1 (fr) * 1989-11-03 1991-05-22 Abbott Laboratories Substrats fluorogéniques pour la détection de l'activité prothéolytique d'enzymes
DK93990D0 (da) 1990-04-17 1990-04-17 Carlsberg As Fluorogene peptider og deres anvendelse ved bestemmelse af enzymatisk aktivitet
GB9310978D0 (en) 1993-05-27 1993-07-14 Zeneca Ltd Compounds
US5508289A (en) * 1994-03-14 1996-04-16 The United States America As Represented By The Department Of Health And Human Services Bis-acridone chemotherapeutic derivatives
US5506115A (en) * 1994-04-29 1996-04-09 G. D. Searle & Co. Reagent and method for determining activity of herpes protease
US5807746A (en) 1994-06-13 1998-09-15 Vanderbilt University Method for importing biologically active molecules into cells
DE69632244T2 (de) 1995-01-30 2005-04-14 Daiichi Pure Chemicals Co. Ltd. Diagnostisches Markierungsmittel
US20010004522A1 (en) 1997-10-28 2001-06-21 Thomas J. Burke Kinase activity measurement using fluorescence polarization
US6194143B1 (en) * 1996-12-27 2001-02-27 Boehringer Ingelheim (Canada) Ltd. Substrates for human cytomegalovirus protease
DE19717904A1 (de) 1997-04-23 1998-10-29 Diagnostikforschung Inst Säurelabile und enzymatisch spaltbare Farbstoffkonstrukte zur Diagnostik mit Nahinfrarotlicht und zur Therapie
CA2298067A1 (fr) 1997-07-24 1999-02-04 Ulo Langel Conjugues de peptides transporteurs et analogues d'acides nucleiques ainsi que leur utilisation
US6133445A (en) 1997-12-17 2000-10-17 Carnegie Mellon University Rigidized trimethine cyanine dyes
US6251583B1 (en) 1998-04-27 2001-06-26 Schering Corporation Peptide substrates for HCV NS3 protease assays
EP1102976A1 (fr) * 1998-08-08 2001-05-30 Imperial Cancer Research Technology Limited Imagerie de duree de vie de fluorescence a des frequences multiples
US6447724B1 (en) * 1998-08-11 2002-09-10 Caliper Technologies Corp. DNA sequencing using multiple fluorescent labels being distinguishable by their decay times
GB9901072D0 (en) * 1999-01-19 1999-03-10 Imp Cancer Res Tech Methods for detecting changes to a macromolecular component of a cell
US6410255B1 (en) * 1999-05-05 2002-06-25 Aurora Biosciences Corporation Optical probes and assays
US6323186B1 (en) 1999-09-17 2001-11-27 Molecular Probes, Inc. Phosphate-bound polyazaindacene derivatives of nucleotides
GB2357084A (en) 1999-12-06 2001-06-13 Amersham Pharm Biotech Uk Ltd A hydrophobic carrier peptide
EP1379284A4 (fr) 2001-01-05 2007-07-25 Gen Hospital Sondes d'imagerie activables
DE10117430A1 (de) 2001-04-06 2002-10-10 Nicole Marme Hochempfindlicher und hochspezifischer Enzymnachweis mit einer Nachweisgrenze bis in den femtomolaren Bereich
GB0113435D0 (en) 2001-06-04 2001-07-25 Amersham Pharm Biotech Uk Ltd Acridone derivatives as labels for fluorescence detection of target materials
GB0113434D0 (en) 2001-06-04 2001-07-25 Amersham Pharm Biotech Uk Ltd Quinacridone derivatives as labels for fluorescence detection of biological materials

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1979000602A1 (fr) * 1978-02-07 1979-08-23 Kabi Ab Substrats facilement dissociables pour la determination quantitative de proteases
US20010005752A1 (en) * 1998-12-21 2001-06-28 Manfred Auer Fluorescent dyes (AIDA) for solid phase and solution phase screening
WO2000073437A1 (fr) * 1999-05-27 2000-12-07 Merck Frosst Canada & Co. Analyses permettant de detecter l'activite de caspases a l'aide de proteines fluorescentes vertes

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
FALLER T ET AL: "A novel acridone derivative for the fluorescence tagging and mass spectrometric sequencing of peptides", CHEMICAL COMMUNICATIONS - CHEMCOM, ROYAL SOCIETY OF CHEMISTRY, GB, vol. 16, 1997, pages 1529 - 1530, XP002251180, ISSN: 1359-7345 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101372632B1 (ko) 2011-08-26 2014-03-14 동국대학교 산학협력단 Ｘｐｇ 엔도뉴클레아제 활성 분석 방법

Also Published As

Publication number Publication date
GB0308910D0 (en) 2003-05-21
EP1497452A2 (fr) 2005-01-19
JP2005527212A (ja) 2005-09-15
GB2388190A (en) 2003-11-05
DE60336217D1 (de) 2011-04-14
US20100184098A1 (en) 2010-07-22
US7727739B2 (en) 2010-06-01
ATE500342T1 (de) 2011-03-15
CA2484467A1 (fr) 2003-10-30
AU2003229914A1 (en) 2003-11-03
JP4713835B2 (ja) 2011-06-29
EP1497452B1 (fr) 2011-03-02
GB2388190B (en) 2004-11-17
US20030228609A1 (en) 2003-12-11
CA2484467C (fr) 2012-01-10
WO2003089663A2 (fr) 2003-10-30
GB0208989D0 (en) 2002-05-29

Similar Documents

Publication Publication Date Title
WO2003089663A3 (fr) Methodes de mesure d'activite enzymatique
WO2004029219A3 (fr) Interference arn basee sur les cellules, et procedes et compositions s'y rapportant
WO2006131928A8 (fr) Nouvelles sequences de nucleotides et d'acides amines, leurs dosages et methodes d'utilisation en vue du diagnostic
WO2007039903A3 (fr) Nouveaux nucleotides et nouvelles sequences d'acides amines, et bioessais et procedes d'utilisation associes a des fins de diagnostic
WO2006138418A3 (fr) Amelioration de la performance cognitive avec des activateurs de sirtuine
WO2004055519A3 (fr) Marqueurs specifiques pour le cancer du pancreas
WO2005014850A3 (fr) Systemes et procedes d'analyse de sequences d'acides nucleiques
IL176478A0 (en) Derivatives of 1-piperazine- and 1-homopiperazine-carboxylates, preparation method thereof and use of same as inhibitors of the faah enzyme
SG161247A1 (en) Variant forms of urate oxidase and use thereof
WO2006063055A3 (fr) Conjugues enzymatiques utilises en tant qu'agents detoxifiants
WO2008033427A3 (fr) Compositions et procédés de détection d'une maladie lysosomale
WO2005076908A3 (fr) Amplification d'acide nucleique ameliore de proteine antigel
WO2006014530A3 (fr) Imagerie de l'activite enzymatique
WO2003003004A3 (fr) Technique d'identification de composes ayant un effet de depletion specifiquement sur des mastocytes
WO2007050795A3 (fr) Inhibiteurs de transglutaminase et procedes d'utilisation
WO2005123913A3 (fr) Compositions d'enzymes thermostables modifiees de façon reversible, et methodes de production et d'utilisation desdites compositions
WO2007075910A3 (fr) Procedes et compositions permettant de detecter l'activite enzymatique
WO2005089505A3 (fr) Detection, isolement et utilisations de la renalase (monoamine oxydase de type c)
WO2006054297A3 (fr) Nouvelles sequences nucleotidiques et d'acides amines, et leurs dosages et leurs procedes d'utilisation pour le diagnostic
WO2008088865A3 (fr) Imagerie optique antisens et de préciblage
WO2005007663A3 (fr) Substrats d'enzymes fluorogenes et leurs utilisations
WO2006104761A3 (fr) Sondes d'hybridation unisequentielles
IL177329A0 (en) Derivatives of heteroaryl-alkylcarbamates, preparation method therof and use of the same as faah enzyme inhibitors
WO2004063339A3 (fr) Essai de liaison de g-quadruplex et composes utilises a cette fin
WO2003050265A3 (fr) Compositions et procedes pour normaliser des analyses

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NI NO NZ OM PH PL PT RO RU SC SD SE SG SK SL TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 2003722751

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 164347

Country of ref document: IL

WWE Wipo information: entry into national phase

Ref document number: 2003229914

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 2484467

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 2003586372

Country of ref document: JP

WWP Wipo information: published in national office

Ref document number: 2003722751

Country of ref document: EP