WO2003083477A3 - Nouveau support solide actif et procede d'immobilisation de surface de composes ou de bibliotheques combinatoires - Google Patents
Nouveau support solide actif et procede d'immobilisation de surface de composes ou de bibliotheques combinatoires Download PDFInfo
- Publication number
- WO2003083477A3 WO2003083477A3 PCT/HU2003/000025 HU0300025W WO03083477A3 WO 2003083477 A3 WO2003083477 A3 WO 2003083477A3 HU 0300025 W HU0300025 W HU 0300025W WO 03083477 A3 WO03083477 A3 WO 03083477A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- solid support
- group
- active solid
- reacted
- drug
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 7
- 239000007787 solid Substances 0.000 title abstract 7
- 150000001875 compounds Chemical class 0.000 title 1
- 229940079593 drug Drugs 0.000 abstract 3
- 239000003814 drug Substances 0.000 abstract 3
- 125000000217 alkyl group Chemical group 0.000 abstract 2
- 238000004873 anchoring Methods 0.000 abstract 2
- 125000003710 aryl alkyl group Chemical group 0.000 abstract 2
- WERYXYBDKMZEQL-UHFFFAOYSA-N butane-1,4-diol Chemical compound OCCCCO WERYXYBDKMZEQL-UHFFFAOYSA-N 0.000 abstract 2
- 239000003153 chemical reaction reagent Substances 0.000 abstract 2
- 229940000406 drug candidate Drugs 0.000 abstract 2
- 238000004519 manufacturing process Methods 0.000 abstract 2
- 238000002493 microarray Methods 0.000 abstract 2
- 239000002516 radical scavenger Substances 0.000 abstract 2
- 239000000126 substance Substances 0.000 abstract 2
- JHWGFJBTMHEZME-UHFFFAOYSA-N 4-prop-2-enoyloxybutyl prop-2-enoate Chemical compound C=CC(=O)OCCCCOC(=O)C=C JHWGFJBTMHEZME-UHFFFAOYSA-N 0.000 abstract 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 abstract 1
- BRLQWZUYTZBJKN-UHFFFAOYSA-N Epichlorohydrin Chemical compound ClCC1CO1 BRLQWZUYTZBJKN-UHFFFAOYSA-N 0.000 abstract 1
- 239000002253 acid Substances 0.000 abstract 1
- 150000007513 acids Chemical class 0.000 abstract 1
- 125000003647 acryloyl group Chemical group O=C([*])C([H])=C([H])[H] 0.000 abstract 1
- 125000003277 amino group Chemical group 0.000 abstract 1
- 125000003118 aryl group Chemical group 0.000 abstract 1
- 125000004432 carbon atom Chemical group C* 0.000 abstract 1
- 238000001035 drying Methods 0.000 abstract 1
- 125000003700 epoxy group Chemical group 0.000 abstract 1
- RTZKZFJDLAIYFH-UHFFFAOYSA-N ether Substances CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 abstract 1
- 125000000524 functional group Chemical group 0.000 abstract 1
- 229910052736 halogen Inorganic materials 0.000 abstract 1
- 150000002367 halogens Chemical class 0.000 abstract 1
- NHBRUUFBSBSTHM-UHFFFAOYSA-N n'-[2-(3-trimethoxysilylpropylamino)ethyl]ethane-1,2-diamine Chemical compound CO[Si](OC)(OC)CCCNCCNCCN NHBRUUFBSBSTHM-UHFFFAOYSA-N 0.000 abstract 1
- 238000002203 pretreatment Methods 0.000 abstract 1
- 125000003107 substituted aryl group Chemical group 0.000 abstract 1
- 238000005406 washing Methods 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
Abstract
L'invention concerne un procédé de production d'un support solide actif convenant à l'ancrage de molécules de médicaments par des liaisons covalentes par les procédés suivants: a) un support solide est préparé à l'avance par prétraitement en présence de solides bases ou acides puis réagit avec un dérivé alcoxy-silane fonctionnalisé par un polyamine, de préférence un 3-(2-(2-aminoéthylamino)éthylamino)propyl-triméthoxy-silane, et est ensuite soumis à un lavage et à un séchage ; b1) le support solide obtenu en a) réagit avec (I) où Z représente une liaison ou un groupe CO ou CNR1 et le groupe R1 représente un alkyle, un aralkyle ou un groupe aryle ; B représente une liaison ou un groupe (CH2)n- ou (CH2)n-O-(CH2)n- ou O-(CH2)n- ou NH-(CH2)n ; A représente un groupe aryle ramifié ou non ramifié ou un groupe aryle ou aralkyle substitué contenant 1 à 5 atomes de carbone et D représente un halogène ou un groupe O-A et n vaut 0, 1, 2, un entier, avec un réactif bifonctionnel, de préférence, ayant réagi avec un chlorure d'acryloyle ou un 1,4-butanédiol diacrylate en présence d'un entraîneur organique ou inorganique ; ou bien B2) le support obtenu en a) réagit avec un réactif bifonctionnel, de préférence, un 1,4- butanédiol diglicidyl-éther ou épichlorohydrine, en présence d'un entraîneur organique ou inorganique contenant des groupes époxy terminaux et un sous-groupe fonctionnel réagissant au groupe amino. L'invention concerne également le support solide actif produit à l'aide des procédés susmentionnés, ainsi que l'application du support solide actif décrit ci-dessus pour la production de microréseaux chimiques et de microréseaux chimiques combinatoires. L'invention concerne enfin un procédé d'immobilisation des « molécules organiques de faible poids moléculaire » de taille inférieure à 1500 Da, de préférence, comprise entre 250 et 750 Da. Ce procédé consiste à ancrer un médicament non modifié et une molécule candidate de médicament sur un support solide actif décrit ci-dessus par l'addition des groupes fonctionnels appropriés du médicament ou de la molécule candidate de médicament, de préférence, exécutée par une technique de liaison robotisée de l'ordre du micromètre.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2003226574A AU2003226574A1 (en) | 2002-03-29 | 2003-03-28 | Active solid support and method for surface immobilization of combinatorial compounds or libraries |
| EP03745343A EP1493031A2 (fr) | 2002-03-29 | 2003-03-28 | Nouveau support solide actif et procede d'immobilisation de surface de composes ou de bibliotheques combinatoires |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| HUP0201091 | 2002-03-29 | ||
| HU0201091A HU226993B1 (en) | 2002-03-29 | 2002-03-29 | Novel active carrier and process for immobilization of combinatorial compounds and compound libraries |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO2003083477A2 WO2003083477A2 (fr) | 2003-10-09 |
| WO2003083477A3 true WO2003083477A3 (fr) | 2004-05-06 |
Family
ID=89980305
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/HU2003/000025 WO2003083477A2 (fr) | 2002-03-29 | 2003-03-28 | Nouveau support solide actif et procede d'immobilisation de surface de composes ou de bibliotheques combinatoires |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP1493031A2 (fr) |
| AU (1) | AU2003226574A1 (fr) |
| HU (1) | HU226993B1 (fr) |
| WO (1) | WO2003083477A2 (fr) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7217512B2 (en) | 2002-05-09 | 2007-05-15 | Corning Incorporated | Reagent and method for attaching target molecules to a surface |
| US7723126B2 (en) * | 2004-03-24 | 2010-05-25 | Wisconsin Alumni Research Foundation | Plasma-enhanced functionalization of inorganic oxide surfaces |
| US20060147943A1 (en) * | 2004-12-30 | 2006-07-06 | Lewis Mark A | Substrates having pendant epoxide groups for binding biomolecules and methods of making and using thereof |
| US7396676B2 (en) | 2005-05-31 | 2008-07-08 | Agilent Technologies, Inc. | Evanescent wave sensor with attached ligand |
| US20070099180A1 (en) * | 2005-10-31 | 2007-05-03 | Robotti Karla M | Evanescent wave sensor with attached ligand |
| HUP0600668A2 (en) | 2006-08-22 | 2008-02-28 | Avicor Kft | Active carrier, process for producing thereof and the use of thereof |
| US8029902B2 (en) | 2006-12-11 | 2011-10-04 | Wisconsin Alumni Research Foundation | Plasma-enhanced functionalization of substrate surfaces with quaternary ammonium and quaternary phosphonium groups |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5919626A (en) * | 1997-06-06 | 1999-07-06 | Orchid Bio Computer, Inc. | Attachment of unmodified nucleic acids to silanized solid phase surfaces |
| US5919523A (en) * | 1995-04-27 | 1999-07-06 | Affymetrix, Inc. | Derivatization of solid supports and methods for oligomer synthesis |
| WO2001001143A2 (fr) * | 1999-06-25 | 2001-01-04 | Motorola Inc. | Procedes et compositions permettant de fixer des biomolecules a des supports solides, a des hydrogels et a des matrices d'hydrogels |
-
2002
- 2002-03-29 HU HU0201091A patent/HU226993B1/hu not_active IP Right Cessation
-
2003
- 2003-03-28 AU AU2003226574A patent/AU2003226574A1/en not_active Abandoned
- 2003-03-28 WO PCT/HU2003/000025 patent/WO2003083477A2/fr not_active Application Discontinuation
- 2003-03-28 EP EP03745343A patent/EP1493031A2/fr not_active Withdrawn
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5919523A (en) * | 1995-04-27 | 1999-07-06 | Affymetrix, Inc. | Derivatization of solid supports and methods for oligomer synthesis |
| US5919626A (en) * | 1997-06-06 | 1999-07-06 | Orchid Bio Computer, Inc. | Attachment of unmodified nucleic acids to silanized solid phase surfaces |
| WO2001001143A2 (fr) * | 1999-06-25 | 2001-01-04 | Motorola Inc. | Procedes et compositions permettant de fixer des biomolecules a des supports solides, a des hydrogels et a des matrices d'hydrogels |
Non-Patent Citations (2)
| Title |
|---|
| BLACKBURN CHRISTOPHER: "Polymer supports for solid-phase organic synthesis.", BIOPOLYMERS, vol. 47, no. 5, 25 January 1998 (1998-01-25), pages 311 - 351, XP002255078, ISSN: 0006-3525 * |
| REHMAN F ET AL: "Immobilization of acrylamide-modified oligonucleotides by co-polymerization", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 27, no. 2, 15 February 1999 (1999-02-15), pages 649 - 655, XP002155808, ISSN: 0305-1048 * |
Also Published As
| Publication number | Publication date |
|---|---|
| HU0201091D0 (fr) | 2002-06-29 |
| HUP0201091A2 (hu) | 2003-12-29 |
| WO2003083477A2 (fr) | 2003-10-09 |
| EP1493031A2 (fr) | 2005-01-05 |
| HU226993B1 (en) | 2010-04-28 |
| HUP0201091A3 (en) | 2007-08-28 |
| AU2003226574A8 (en) | 2003-10-13 |
| AU2003226574A1 (en) | 2003-10-13 |
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