WO2003079806A1 - An improved process for the preparation of soy protein concentrate with improved functional properties - Google Patents
An improved process for the preparation of soy protein concentrate with improved functional properties Download PDFInfo
- Publication number
- WO2003079806A1 WO2003079806A1 PCT/IB2002/001128 IB0201128W WO03079806A1 WO 2003079806 A1 WO2003079806 A1 WO 2003079806A1 IB 0201128 W IB0201128 W IB 0201128W WO 03079806 A1 WO03079806 A1 WO 03079806A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- protein concentrate
- soy
- soy protein
- flakes
- range
- Prior art date
Links
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/14—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/14—Vegetable proteins
- A23J3/16—Vegetable proteins from soybean
Definitions
- the present invention particularly relates to an improved process for the preparation of soy protein concentrate from defatted soy flakes with improved functional properties.
- Vegetable proteins have formed part of man's diet in developed countries, it is the vegetable sources provide the essential nutrients. Vegetable proteins contribute significant percentage of daily requirement of protein. The richest sources are oilseeds, legumes and cereals. Soybeans are generally acknowledged as high quality vegetable protein source and are the most abundant. Annual production of soy in India is around 6.8 MT. Soy bean has high levels of important nutrients. It contains about 20% oil and 40% protein with very good proportions of essential amino acids. The remaining 40% is carbohydrates which is indigestible and functions as roughage or dietary fiber. Soybean is also good source of vitamins and minerals.
- soy beans soy beans, soy flour(full fat or defatted), soy protein concentrates and isolates.
- soy protein preparations are characterized by undesirable beany and bitter flavour which limiting their use in a variety of food products.
- undesirable flavour it also contains flatus producing indigestible carbohydrates; stachyose and raffinose.
- the raw soy bean also contains some of the antinutritional factors such as trypsin inhibitors, urease and lipoxygenase activity.
- Soy proteins concentrates are available in granular form, ground flour, textured pieces of various sizes and in spray dried form. Soy proteins concentrate find application in a variety of foods especially as a functional ingredient in bakery and as extenders in meat Industries. Also found use in major non-meat preparations like non-dairy products, health foods and pasta products.
- soy protein concentrate is prepared from soy beans which have been genetically modified to reduce or eliminate the raffinose, stachyose, lipoxygenase content and associated off flavours.
- the protein is solubilised using alkali and dried the slurry to get a protein content of not less than 60% on dry basis.
- the drawback of the process is, genetically modified soy seeds are used and protein content of concentrate is low.
- US patent 6313273 Thomas, R. L. N. et al, 2001, Abbot Laboratories, wherein soy protein concentrate is produced by a process enzyme treatment combined with ultrafiltration.
- the main object of the present invention is to provide an improved process for the preparation of soy protein concentrate with improved functional properties.
- Another object of the present invention is to provide a protein concentrate to eliminate or to reduce lipoxygenase, urease and trypsin inhibitor activity.
- Yet another object of the present invention is to obtain soy concentrate with reduced raffinose and stachyose with a protein content of 70%.
- the invention particularly relates to the preparation of soy protein concentrate from defatted soy flakes with improved functional properties. The preparation involves the process of autoclaving, leaching, and drying of defatted soy flakes. Detailed description of the Invention
- the present invention provides a process for the preparation of soy protein concentrate from defatted soy flakes with an improved functional properties, the said process comprising steps of: a) autoclaving defatted soy flakes at a temperature range of 100°-110° C for a period of 10-15 minutes, b) leaching by percolating water through autoclaved defatted soy flakes of step (a) in two stages, c) draining excess water from soy flakes of step (b), d) drying the wet flakes of step (c) in a cross flow drier, and e) pulverizing and sieving the dried soy flakes of step (d) to get required soy protein concentrate
- a starting material as defatted soy flakes having fat content of around 1%.
- the drying is effective in a fluidized bed dryer having inlet temperature 80-85°C and outlet temperature 35- 40°C for a period of 2 5 to 30min.
- the protein concentrate may have a protein content of 70-72%.
- the protein concentrate obtained has a particle size of 220 ⁇ -225 ⁇ .
- the complete inactivation of lipoxygenase, and reduction of the urease activity may be 0.2 to 0.5 units and trypsin inhibitor activity may be 6-8 TIU /mg material may be achieved.
- raffinose and stachyose contents may be reduced to less then
- product may have a water absorption capacity of 270 ⁇ 8 g/lOOg and a fat absorption capacity of 122 ⁇ 8 g/lOOg.
- the hexanal content may be reduced from 64 ⁇ mole/g to 22 ⁇ mole/g of the original.
- the protein concentrate may have 50% reduction in beany flavour of the original by sensory evaluation.
- the protein concentrate may retain all the amino acid content present in the starting material.
- the protein concentrate may have improved protein digestibility (88%) compared to starting material (80%).
- the protein concentrate will have good shelf life of 180 days.
- One more embodiment of the invention provides a soy protein concentrate obtained from defatted soy flakes having the following improved functional properties: a) soy protein concentrate has protein content in the range of 70-72%. b) the soy protein concentrate has reduced urease activity of 0.2-0.5 units. c) the soy protein concentrate has trypsin inhibitory activity in the range of 6-8 TlU/mg. d) the soy protein concentrate has raffinose and stachyose contents reduced to 10% of the original content. e) water absorption capacity is 270 + 8 gms/lOOgm and fat absorption capacity is 122 + 8 gm/100 gm. f) particle size is in the range of 220 ⁇ -225 ⁇ .
- hexanal content is reduced from to 22 ⁇ mole/gm from 64 ⁇ mole/gm.
- the beany flavor of the soy protein concentrate is by 50%.
- the product has all the amino acid contents retained as present in the starting defatted soy flakes.
- the product has improved protein digestibility up to 88% .
- the product has a shelf life of 180 days. Novelty of the process:
- the novelty of an improved process for the preparation of soy protein concentrate with improved functional properties are: II. The use of optimum conditions like temperature, moisture and pressure to maximize destruction of trypsin inhibitor activities, minimize the reduction of solubility and loss of essential amino acids. III. The defatted soy flakes are moist heat treated to denature protein and render them insoluble in water. IV. Soluble carbohydrates and salts are removed from insoluble protein and polysaccharide material by water leaching and dried to yield concentrate with protein content of 69-72%. V. The raffinose and stachyose content is reduced to less than 5-10% of its original value. VI. The protein concentrate has improved digestibility of proteins of 88%.
- the process improved the water and fat absorption capacities.
- the main advantages of the invention are: 1.
- the protein content of the protein concentrate ranges from 69-72%.
- the yield of protein concentrate ranged from 70-75%.
- the process reduces or eliminates the Lipoxygenase, urease and trypsin inhibitor activities in the product. 4. The process minimizes the flatus producing oligosaccharides, raffinose and stachyose by 96 and 90% respectively.
- the process improves the invitro digestibility of protein to 88%.
- Example 1 The beany flavour present in the starting material is reduced by 50%. 7. The process improves the functional properties such as water and fat absorption capacities. 8. The process preserves all the essential amino acids present in the original material.
- Example 2 One kg defatted soy flakes were spread over stainless steel trays and autoclaved at a temperature of 110°C for 15 min. The flakes were soaked in water in a ratio of 1:4 (w/v) in percolating columns for 2h and removed. Repeated the extraction in a ratio of 1 :3 (w/v). The wet flakes were dried at 60°C in a through flow drier. The moisture and protein content of the protein concentrate obtained was 4 and 67.7% respectively with an yield of 72%.
- Example 3 Two hundred grams of defatted soy flakes were spread over stainless steel trays and autoclaved at a temperature of 110°C for 15 min. The flakes were soaked in water in a ratio of 1 :4 (w/v) for 2h and removed. The extraction is repeated in a ratio of 1:3 (w/v). The wet flakes were dried at 85°C in a cross flow drier. The moisture and protein content of the soy protein concentrate obtained is 6 and 68.8% respectively with a yield of 70%.
- Example 4 Two hundred grams of defatted soy flakes were spread over stainless steel trays and autoclaved at a temperature of 110°C for 15 min. The flakes were soaked in water in a ratio of 1 :4 (w/v) for 2h and removed. The extraction is repeated in a ratio of 1:3 (w/v). The wet flakes were dried at 85°C in a cross flow drier. The moisture and protein content of the soy protein concentrate obtained is 6 and 68.8% respectively with
- Example 5 Five hundred grams of defatted soy flakes were spread over stainless steel trays and autoclaved at a temperature of 110°C for 15 min. The flakes were soaked in water in a ratio of 1:4 (w/v) for 2h and drained. The process is repeated with a ratio of 1 :3 (w/v). The wet flakes were dried at 90°C in a cabinet drier. The moisture and protein content of the protein concentrate obtained is 6.3 and 65.9% respectively with a yield of 76%. The product is evaluated for hexanal content reduced from 64 ⁇ mole/g to 22 ⁇ mole/g of the original material, and the beany flavour reduced by 55%.
- Example 6 4.5 kgs of defatted soy flakes were spread over stainless steel trays and autoclaved at a temperature of 110°C for 15 min. The flakes were soaked in water in a ratio of 1:4 (w/v) for 2h and drained. The extraction is repeated in a ratio of 1 :3 (w/v). The wet flakes were dried using fluid bed drier (inlet temp.85°C and outlet temp 40 °C) for 25-30min. The moisture and protein content of the protein concentrate obtained is 6.2% and 68.5% respectively with a yield of 75%.
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Beans For Foods Or Fodder (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Peptides Or Proteins (AREA)
Abstract
Description
Claims
Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU2002247914A AU2002247914A1 (en) | 2002-03-25 | 2002-03-25 | An improved process for the preparation of soy protein concentrate with improved functional properties |
JP2003577649A JP4176646B2 (en) | 2002-03-25 | 2002-03-25 | Improved process for the preparation of soy protein with improved functional properties |
PCT/IB2002/001128 WO2003079806A1 (en) | 2002-03-25 | 2002-03-25 | An improved process for the preparation of soy protein concentrate with improved functional properties |
CNB028286278A CN100370917C (en) | 2002-03-25 | 2002-03-25 | Improved process for the preparation of soy protein concentrate with improved functional properties |
CA2480250A CA2480250C (en) | 2002-03-25 | 2002-03-25 | An improved process for the preparation of soy protein concentrate with improved functional properties |
KR1020047015244A KR100897450B1 (en) | 2002-03-25 | 2002-03-25 | An Improved Process for the Preparation of Soy Protein Concentrate with Improved Functional Properties |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/IB2002/001128 WO2003079806A1 (en) | 2002-03-25 | 2002-03-25 | An improved process for the preparation of soy protein concentrate with improved functional properties |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2003079806A1 true WO2003079806A1 (en) | 2003-10-02 |
Family
ID=28053163
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/IB2002/001128 WO2003079806A1 (en) | 2002-03-25 | 2002-03-25 | An improved process for the preparation of soy protein concentrate with improved functional properties |
Country Status (6)
Country | Link |
---|---|
JP (1) | JP4176646B2 (en) |
KR (1) | KR100897450B1 (en) |
CN (1) | CN100370917C (en) |
AU (1) | AU2002247914A1 (en) |
CA (1) | CA2480250C (en) |
WO (1) | WO2003079806A1 (en) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2007149095A1 (en) * | 2006-06-21 | 2007-12-27 | Solae, Llc | Bland tasting soy protein isolate and processes for making same |
WO2007149093A1 (en) * | 2006-06-21 | 2007-12-27 | Solae, Llc | Bland tasting soy protein isolante and processes for making same |
WO2018087168A1 (en) | 2016-11-10 | 2018-05-17 | Hamlet Protein A/S | A method for the manufacture of a processed soy protein product |
US11627748B2 (en) | 2016-11-10 | 2023-04-18 | Hamlet Protein A/S | Processed protein product |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20110119784A (en) * | 2009-02-11 | 2011-11-02 | 버콘 뉴트라사이언스 (엠비) 코포레이션 | Preparation of soy protein product using water extraction ("s803") |
BRPI1008535B1 (en) * | 2009-02-11 | 2021-11-23 | Burcon Nutrascience (Mb) Corp | METHOD OF PRODUCTION OF A SOYBEAN PROTEIN PRODUCT USING CALCIUM CHLORIDE EXTRACTION |
KR101188792B1 (en) | 2010-06-15 | 2012-10-10 | 씨제이제일제당 주식회사 | A process for separating protein from food |
RU2769570C1 (en) * | 2021-03-31 | 2022-04-04 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Чеченский государственный университет" | Method for producing "yalt" full-fat soy |
CN115176860A (en) * | 2022-05-24 | 2022-10-14 | 山东凯斯达机械制造有限公司 | Method for preparing functional concentrated protein from flaky soybean high-temperature meal |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3305984A1 (en) * | 1982-11-22 | 1984-05-24 | VEB Schwermaschinenbau-Kombinat "Ernst Thälmann" Magdeburg, DDR 3011 Magdeburg | Process for recovering proteins |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3971856A (en) * | 1975-03-03 | 1976-07-27 | Archer Daniels Midland Company | Process for preparing soy protein concentrate |
US4493854A (en) * | 1983-09-20 | 1985-01-15 | The United States Of America As Represented By The Secretary Of Agriculture | Production of defatted soybean products by supercritical fluid extraction |
CN1068713A (en) * | 1991-07-18 | 1993-02-10 | 长春市工程食品研究所 | Take off raw meat, remove and expand, go enzyme soyabean protein powder method for making |
-
2002
- 2002-03-25 KR KR1020047015244A patent/KR100897450B1/en not_active IP Right Cessation
- 2002-03-25 JP JP2003577649A patent/JP4176646B2/en not_active Expired - Fee Related
- 2002-03-25 WO PCT/IB2002/001128 patent/WO2003079806A1/en active Application Filing
- 2002-03-25 CN CNB028286278A patent/CN100370917C/en not_active Expired - Fee Related
- 2002-03-25 CA CA2480250A patent/CA2480250C/en not_active Expired - Fee Related
- 2002-03-25 AU AU2002247914A patent/AU2002247914A1/en not_active Abandoned
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3305984A1 (en) * | 1982-11-22 | 1984-05-24 | VEB Schwermaschinenbau-Kombinat "Ernst Thälmann" Magdeburg, DDR 3011 Magdeburg | Process for recovering proteins |
Non-Patent Citations (4)
Title |
---|
LUSAS E W ET AL: "SOY PROTEIN PRODUCTS: PROCESSING AND USE", JOURNAL OF NUTRITION, WISTAR INSTITUTE OF ANATOMY AND BIOLOGY, PHILADELPHIA, PA,, US, vol. 1995, no. 3, SUPPL, 1995, pages 573S - 580S, XP001064997, ISSN: 0022-3166 * |
OHREN J A ET AL: "Process and product characteristics for soya concentrates and isolates.", JOURNAL OF THE AMERICAN OIL CHEMISTS' SOCIETY 1981 GRAIN PROCESSING CORP., MUSCATINE, IOWA 52761, USA, vol. 58, no. 3, pages 333 - 335, XP002219401 * |
SNYDER H E ET AL: "Soybean Utilization", 1987, SOYBEAN UTILIZATION, NEW YORK, VAN NOSTRAND REINHOLD, US, PAGE(S) 74-86,130-133, NEW YORK, US, XP002201822 * |
WANG L C: "Ultrasonic extraction of a heat-labile 7S protein fraction from autoclaved, defatted soybean flakes.", JOURNAL OF FOOD SCIENCE 1984 USDA, ARS, N. REG. RES. CENT., 1815 N. UNIV. STREET, PEORIA, ILLINOIS 61604, USA, vol. 49, no. 2, pages 551 - 554, XP002219400 * |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2007149095A1 (en) * | 2006-06-21 | 2007-12-27 | Solae, Llc | Bland tasting soy protein isolate and processes for making same |
WO2007149093A1 (en) * | 2006-06-21 | 2007-12-27 | Solae, Llc | Bland tasting soy protein isolante and processes for making same |
WO2018087168A1 (en) | 2016-11-10 | 2018-05-17 | Hamlet Protein A/S | A method for the manufacture of a processed soy protein product |
US11627748B2 (en) | 2016-11-10 | 2023-04-18 | Hamlet Protein A/S | Processed protein product |
Also Published As
Publication number | Publication date |
---|---|
JP4176646B2 (en) | 2008-11-05 |
KR100897450B1 (en) | 2009-05-14 |
KR20040097222A (en) | 2004-11-17 |
CA2480250C (en) | 2010-02-16 |
CN100370917C (en) | 2008-02-27 |
CA2480250A1 (en) | 2003-10-02 |
CN1622759A (en) | 2005-06-01 |
JP2005520529A (en) | 2005-07-14 |
AU2002247914A1 (en) | 2003-10-08 |
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