WO2003078454A3 - Polymer supported reagents for natural products purification - Google Patents

Polymer supported reagents for natural products purification Download PDF

Info

Publication number
WO2003078454A3
WO2003078454A3 PCT/EP2003/002635 EP0302635W WO03078454A3 WO 2003078454 A3 WO2003078454 A3 WO 2003078454A3 EP 0302635 W EP0302635 W EP 0302635W WO 03078454 A3 WO03078454 A3 WO 03078454A3
Authority
WO
WIPO (PCT)
Prior art keywords
polymer supported
natural products
reagents
supported reagents
purification
Prior art date
Application number
PCT/EP2003/002635
Other languages
French (fr)
Other versions
WO2003078454A2 (en
Inventor
Florian Thaler
Moreno Zamai
Original Assignee
Pharmacia Italia Spa
Florian Thaler
Moreno Zamai
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pharmacia Italia Spa, Florian Thaler, Moreno Zamai filed Critical Pharmacia Italia Spa
Priority to JP2003576458A priority Critical patent/JP2005535567A/en
Priority to AU2003218755A priority patent/AU2003218755A1/en
Priority to CA002479355A priority patent/CA2479355A1/en
Priority to EP03712004A priority patent/EP1487862A2/en
Priority to BR0308577-5A priority patent/BR0308577A/en
Priority to MXPA04009084A priority patent/MXPA04009084A/en
Publication of WO2003078454A2 publication Critical patent/WO2003078454A2/en
Publication of WO2003078454A3 publication Critical patent/WO2003078454A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K17/00—Carrier-bound or immobilised peptides; Preparation thereof
    • C07K17/02—Peptides being immobilised on, or in, an organic carrier
    • C07K17/06—Peptides being immobilised on, or in, an organic carrier attached to the carrier via a bridging agent
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
  • Peptides Or Proteins (AREA)

Abstract

There is provided a method for the purification of bio-molecules after chemical modification using one or more insoluble polymer supported reagents. More particularly, the present invention provides a method for the fast purification of labeled bio-molecules using insoluble polymer supported quenching reagents. There are also provided novel polymer supported reagents of the formula (I): Rs-Sp-Re, wherein Rs is an insoluble polymer, Re is one or more reagent residues and Sp is one or more chemically robust spacer that join Rs and Re; and a process for their preparation.
PCT/EP2003/002635 2002-03-20 2003-03-12 Polymer supported reagents for natural products purification WO2003078454A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
JP2003576458A JP2005535567A (en) 2002-03-20 2003-03-12 Polymer-supported reagents for natural product purification
AU2003218755A AU2003218755A1 (en) 2002-03-20 2003-03-12 Polymer supported reagents for natural products purification
CA002479355A CA2479355A1 (en) 2002-03-20 2003-03-12 Polymer supported reagents for natural products purification
EP03712004A EP1487862A2 (en) 2002-03-20 2003-03-12 Polymer supported reagents for natural products purification
BR0308577-5A BR0308577A (en) 2002-03-20 2003-03-12 Polymer Supported Reagents for Natural Product Purification
MXPA04009084A MXPA04009084A (en) 2002-03-20 2003-03-12 Polymer supported reagents for natural products purification.

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP02076087.2 2002-03-20
EP02076087 2002-03-20

Publications (2)

Publication Number Publication Date
WO2003078454A2 WO2003078454A2 (en) 2003-09-25
WO2003078454A3 true WO2003078454A3 (en) 2004-04-01

Family

ID=27838102

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2003/002635 WO2003078454A2 (en) 2002-03-20 2003-03-12 Polymer supported reagents for natural products purification

Country Status (7)

Country Link
EP (1) EP1487862A2 (en)
JP (1) JP2005535567A (en)
AU (1) AU2003218755A1 (en)
BR (1) BR0308577A (en)
CA (1) CA2479355A1 (en)
MX (1) MXPA04009084A (en)
WO (1) WO2003078454A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8038983B2 (en) * 2003-07-29 2011-10-18 Immunomedics, Inc. Fluorinated carbohydrate conjugates
IL212911A0 (en) * 2011-05-16 2011-07-31 Omrix Biopharmaceuticals Ltd Immunoglobulin reduced in thrombogenic contaminants and preparation thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997042230A1 (en) * 1996-05-03 1997-11-13 Warner-Lambert Company Rapid purification by polymer supported quench
WO2001019484A1 (en) * 1999-09-13 2001-03-22 Dyax Corporation Purification device and purification method

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997042230A1 (en) * 1996-05-03 1997-11-13 Warner-Lambert Company Rapid purification by polymer supported quench
US6306959B1 (en) * 1996-05-03 2001-10-23 Warner Lambert Company Rapid purification by polymer supported quench
US6506701B1 (en) * 1996-05-03 2003-01-14 Warner-Lambert Company Rapid purification by polymer supported quench
WO2001019484A1 (en) * 1999-09-13 2001-03-22 Dyax Corporation Purification device and purification method

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
NICEWONGER R B ET AL: "Alternative base matrices for solid phase quenching reagents", TETRAHEDRON LETTERS, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 41, no. 14, April 2000 (2000-04-01), pages 2323 - 2326, XP004193757, ISSN: 0040-4039 *

Also Published As

Publication number Publication date
BR0308577A (en) 2005-01-04
WO2003078454A2 (en) 2003-09-25
CA2479355A1 (en) 2003-09-25
EP1487862A2 (en) 2004-12-22
AU2003218755A1 (en) 2003-09-29
JP2005535567A (en) 2005-11-24
MXPA04009084A (en) 2004-12-06

Similar Documents

Publication Publication Date Title
WO2004037418A3 (en) Catalysts, in microchannel apparatus, and reactions using same
WO2006138666A3 (en) Iterated branching reaction pathways via nucleic acid-mediated chemistry
WO2005103073A3 (en) Td antigens
WO2004078907A3 (en) Streptococcus pyogenes antigens
ATE551352T1 (en) PEPTIDE ACCEPTOR BINDING METHOD
WO2003100012A3 (en) Microarrays and method for running hybridization reaction for multiple samples on a single microarray
WO2004092209A3 (en) S. pneumoniae antigens
WO2005043154A3 (en) High density reaction chambers and methods of use
WO2006044078A3 (en) Apparatus and method for analysis of molecules
WO2004097369A3 (en) Fragmentation-based methods and systems for de novo sequencing
GB0207975D0 (en) Isolating nucleic acid
WO2006054297A3 (en) Novel nucleotide and amino acid sequences, and assays and methods of use thereof for diagnosis
TW200602291A (en) Method of preparing 1-acetyl-1-chlorocyclopropane
WO2004106367A3 (en) Enterococcus antigens
WO2003078454A3 (en) Polymer supported reagents for natural products purification
WO2004094467A3 (en) H. pylori antigens
WO2002053771A3 (en) Detection of pathogenic bacteria
WO2000063435A3 (en) Method of identifying toxic agents using differential gene expression
WO2003020005A3 (en) Lp mammalian proteins; related reagents
AU2003270172A1 (en) Pentafluoroethane production method
DE60109759D1 (en) A METHOD OF PREPARING MIXTURES OF METHYLENEDIANILINE AND ITS HIGH HOMOLOGOUS PRODUCTS
ATE412062T1 (en) METHOD AND MEANS FOR DETECTING ENZYMATIC CLEAVING AND LINKING REACTIONS
WO2001094924A3 (en) Method for analysing enzyme-catalysed reactions using maldi-tof mass spectrometry
WO2002074906A3 (en) Lp mammalian proteins; related reagents
WO2004031724A3 (en) Apparatus and method for expression and capture of biomolecules and complexes on adsorbent surfaces

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NI NO NZ OM PH PL PT RO RU SC SD SE SG SK SL TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 2479355

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: PA/a/2004/009084

Country of ref document: MX

WWE Wipo information: entry into national phase

Ref document number: 2003576458

Country of ref document: JP

WWE Wipo information: entry into national phase

Ref document number: 2003712004

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2003712004

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 2003712004

Country of ref document: EP