WO2003060127A3 - Genes and proteins involved in the biosynthesis of lipopeptides - Google Patents

Genes and proteins involved in the biosynthesis of lipopeptides Download PDF

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Publication number
WO2003060127A3
WO2003060127A3 PCT/CA2002/002021 CA0202021W WO03060127A3 WO 2003060127 A3 WO2003060127 A3 WO 2003060127A3 CA 0202021 W CA0202021 W CA 0202021W WO 03060127 A3 WO03060127 A3 WO 03060127A3
Authority
WO
WIPO (PCT)
Prior art keywords
lipopeptides
biosynthesis
genes
proteins involved
lipopeptide
Prior art date
Application number
PCT/CA2002/002021
Other languages
French (fr)
Other versions
WO2003060127A2 (en
Inventor
Chris M Farnet
Alfredo Staffa
Emmanuel Zazopoulos
Original Assignee
Ecopia Biosciences Inc
Chris M Farnet
Alfredo Staffa
Emmanuel Zazopoulos
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ecopia Biosciences Inc, Chris M Farnet, Alfredo Staffa, Emmanuel Zazopoulos filed Critical Ecopia Biosciences Inc
Priority to AU2002351636A priority Critical patent/AU2002351636A1/en
Priority to EP02787309A priority patent/EP1458868A2/en
Publication of WO2003060127A2 publication Critical patent/WO2003060127A2/en
Publication of WO2003060127A3 publication Critical patent/WO2003060127A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/36—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Actinomyces; from Streptomyces (G)

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Genes and proteins involved in the biosynthesis of lipopeptides by microorganisms, in particular the nucleic acids forming the biosynthetic locus for the A54145 lipopeptide from Streptomyces fradiae and the A54145-like lipopeptide from Streptomyces refuineus. These nucleic acids can be used to make expression constructs and transformed host cells for the production of lipopeptides. The genes and proteins allow direct manipulation of lipopeptides and related chemical structures via chemical engineering of the proteins involved in the biosynthesis of A54145.
PCT/CA2002/002021 2001-12-26 2002-12-24 Genes and proteins involved in the biosynthesis of lipopeptides WO2003060127A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2002351636A AU2002351636A1 (en) 2001-12-26 2002-12-24 Genes and proteins involved in the biosynthesis of lipopeptides
EP02787309A EP1458868A2 (en) 2001-12-26 2002-12-24 Genes and proteins involved in the biosynthesis of lipopeptides

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US34213301P 2001-12-26 2001-12-26
US60/342,133 2001-12-26
US37278902P 2002-04-17 2002-04-17
US60/372,789 2002-04-17

Publications (2)

Publication Number Publication Date
WO2003060127A2 WO2003060127A2 (en) 2003-07-24
WO2003060127A3 true WO2003060127A3 (en) 2004-04-29

Family

ID=26992837

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/CA2002/002021 WO2003060127A2 (en) 2001-12-26 2002-12-24 Genes and proteins involved in the biosynthesis of lipopeptides
PCT/CA2002/002022 WO2003060128A2 (en) 2001-12-26 2002-12-24 Compositions, methods and systems for discovery of lipopeptides

Family Applications After (1)

Application Number Title Priority Date Filing Date
PCT/CA2002/002022 WO2003060128A2 (en) 2001-12-26 2002-12-24 Compositions, methods and systems for discovery of lipopeptides

Country Status (5)

Country Link
EP (2) EP1458868A2 (en)
JP (1) JP2005514067A (en)
AU (2) AU2002351636A1 (en)
CA (1) CA2412226A1 (en)
WO (2) WO2003060127A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102007017861A1 (en) * 2007-04-13 2008-10-16 Philipps-Universität Marburg Protein for the chemoenzymatic production of L-threo-hydroxyaspartate

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0337731A2 (en) * 1988-04-11 1989-10-18 Eli Lilly And Company Peptide antibiotics
WO2000040704A1 (en) * 1999-01-06 2000-07-13 The Regents Of The University Of California Bleomycin gene cluster components and their uses
WO2001030985A1 (en) * 1999-10-22 2001-05-03 Marahiel Mohamed A Tailored peptide synthetases, a method for their production and the use thereof
WO2001053533A2 (en) * 2000-01-21 2001-07-26 Kosan Biosciences, Inc. Method for cloning polyketide synthase genes
CA2352451A1 (en) * 2001-07-24 2001-10-28 Ecopia Biosciences Inc. High throughput method for discovery of gene clusters

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002526107A (en) * 1998-10-07 2002-08-20 マキシジェン， インコーポレイテッド DNA shuffling to generate nucleic acids for mycotoxin detoxification
ATE292684T1 (en) * 2000-10-13 2005-04-15 Ecopia Biosciences Inc RAMOPLANIN BIOSYNTHESIS GENE CLUSTERS
WO2002059322A2 (en) * 2000-10-17 2002-08-01 Cubist Pharmaceuticlas, Inc. Compositions and methods relating to the daptomycin biosynthetic gene cluster

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0337731A2 (en) * 1988-04-11 1989-10-18 Eli Lilly And Company Peptide antibiotics
WO2000040704A1 (en) * 1999-01-06 2000-07-13 The Regents Of The University Of California Bleomycin gene cluster components and their uses
WO2001030985A1 (en) * 1999-10-22 2001-05-03 Marahiel Mohamed A Tailored peptide synthetases, a method for their production and the use thereof
WO2001053533A2 (en) * 2000-01-21 2001-07-26 Kosan Biosciences, Inc. Method for cloning polyketide synthase genes
CA2352451A1 (en) * 2001-07-24 2001-10-28 Ecopia Biosciences Inc. High throughput method for discovery of gene clusters

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
BALTZ, R. H. ET AL: "Genetics of lipopeptide antibiotic biosynthesis in Streptomyces fradiae A54145 and Streptomyces roseosporus A21978", DEVELOPMENTS IN INDUSTRIAL MICROBIOLOGY SERIES (1997), 34, 93-98, XP001146956 *
DOEKEL S ET AL: "Biosynthesis of natural products on modular peptide synthetases.", METABOLIC ENGINEERING. UNITED STATES JAN 2001, vol. 3, no. 1, January 2001 (2001-01-01), pages 64 - 77, XP002237795, ISSN: 1096-7176 *
HOSTEDT T.J. ET AL.: "Cloning and DNA sequence scanning of the Streptomyces fradiae lipopeptide antibiotic biosynthetic gene cluster", ABSTR.GEN.MEET.AM.SOC.MICROBIOL.;(1996) 96 MEET., 366 CODEN: 0005P ISSN: 0067-2777 AMERICAN SOCIETY FOR MICROBIOLOGY, 96TH GENERAL MEETING, NEW ORLEANS, LA, 19-23 MAY, 1996., XP002237796 *
MCHENNEY MARGARET A ET AL: "Molecular cloning and physical mapping of the daptomycin gene cluster from Streptomyces roseosporus.", JOURNAL OF BACTERIOLOGY, vol. 180, no. 1, January 1998 (1998-01-01), pages 143 - 151, XP002237794, ISSN: 0021-9193 *
TANG L ET AL: "CLONING AND HETEROLOGOUS EXPRESSION OF THE EPOTHILONE GENE CLUSTER", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE,, US, vol. 287, no. 5453, 2000, pages 640 - 642, XP000910114, ISSN: 0036-8075 *
WESSELS PETRA ET AL: "Biosynthesis of acylpeptidolactones of the daptomycin type: A comparative analysis of peptide synthetases forming A21978C and A54145.", EUROPEAN JOURNAL OF BIOCHEMISTRY, vol. 242, no. 3, 1996, pages 665 - 673, XP001147704, ISSN: 0014-2956 *

Also Published As

Publication number Publication date
WO2003060128A2 (en) 2003-07-24
JP2005514067A (en) 2005-05-19
CA2412226A1 (en) 2003-06-22
AU2002351637A1 (en) 2003-07-30
AU2002351636A1 (en) 2003-07-30
WO2003060127A2 (en) 2003-07-24
EP1458868A2 (en) 2004-09-22
EP1461434A2 (en) 2004-09-29
WO2003060128A3 (en) 2004-06-10

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