WO2003060128A3 - Compositions, methods and systems for discovery of lipopeptides - Google Patents

Compositions, methods and systems for discovery of lipopeptides Download PDF

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Publication number
WO2003060128A3
WO2003060128A3 PCT/CA2002/002022 CA0202022W WO03060128A3 WO 2003060128 A3 WO2003060128 A3 WO 2003060128A3 CA 0202022 W CA0202022 W CA 0202022W WO 03060128 A3 WO03060128 A3 WO 03060128A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
polypeptide
systems
lipopeptides
discovery
Prior art date
Application number
PCT/CA2002/002022
Other languages
French (fr)
Other versions
WO2003060128A2 (en
Inventor
Chris M Farnet
Alfredo Staffa
Emmanuel Zazopoulos
Original Assignee
Ecopia Biosciences Inc
Chris M Farnet
Alfredo Staffa
Emmanuel Zazopoulos
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ecopia Biosciences Inc, Chris M Farnet, Alfredo Staffa, Emmanuel Zazopoulos filed Critical Ecopia Biosciences Inc
Priority to EP02787310A priority Critical patent/EP1461434A2/en
Priority to AU2002351637A priority patent/AU2002351637A1/en
Priority to JP2003560212A priority patent/JP2005514067A/en
Publication of WO2003060128A2 publication Critical patent/WO2003060128A2/en
Publication of WO2003060128A3 publication Critical patent/WO2003060128A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/36—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Actinomyces; from Streptomyces (G)

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The invention relates to isolated polypeptides involved in lipopeptide biosynthesis and polynucleotides encoding such polypeptides. In particular, the isolated polypeptide may be an acyl-specific C-domain, an adenylating enzyme, or an acyl carrier. The invention also relates to methods for detecting a polypeptide involved in lipopeptide biosynthesis or a polynucleotide encoding such a polypeptide, as well as relevant useful computer readable medium and computer systems.
PCT/CA2002/002022 2001-12-26 2002-12-24 Compositions, methods and systems for discovery of lipopeptides WO2003060128A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP02787310A EP1461434A2 (en) 2001-12-26 2002-12-24 Compositions, methods and systems for discovery of lipopeptides
AU2002351637A AU2002351637A1 (en) 2001-12-26 2002-12-24 Compositions, methods and systems for discovery of lipopeptides
JP2003560212A JP2005514067A (en) 2001-12-26 2002-12-24 Compositions, methods and systems for finding lipopeptides

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US34213301P 2001-12-26 2001-12-26
US60/342,133 2001-12-26
US37278902P 2002-04-17 2002-04-17
US60/372,789 2002-04-17

Publications (2)

Publication Number Publication Date
WO2003060128A2 WO2003060128A2 (en) 2003-07-24
WO2003060128A3 true WO2003060128A3 (en) 2004-06-10

Family

ID=26992837

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/CA2002/002021 WO2003060127A2 (en) 2001-12-26 2002-12-24 Genes and proteins involved in the biosynthesis of lipopeptides
PCT/CA2002/002022 WO2003060128A2 (en) 2001-12-26 2002-12-24 Compositions, methods and systems for discovery of lipopeptides

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/CA2002/002021 WO2003060127A2 (en) 2001-12-26 2002-12-24 Genes and proteins involved in the biosynthesis of lipopeptides

Country Status (5)

Country Link
EP (2) EP1458868A2 (en)
JP (1) JP2005514067A (en)
AU (2) AU2002351636A1 (en)
CA (1) CA2412226A1 (en)
WO (2) WO2003060127A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102007017861A1 (en) * 2007-04-13 2008-10-16 Philipps-Universität Marburg Protein for the chemoenzymatic production of L-threo-hydroxyaspartate

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000020573A2 (en) * 1998-10-07 2000-04-13 Maxygen, Inc. Dna shuffling to produce nucleic acids for mycotoxin detoxification
WO2000040704A1 (en) * 1999-01-06 2000-07-13 The Regents Of The University Of California Bleomycin gene cluster components and their uses
WO2001030985A1 (en) * 1999-10-22 2001-05-03 Marahiel Mohamed A Tailored peptide synthetases, a method for their production and the use thereof
CA2352451A1 (en) * 2001-07-24 2001-10-28 Ecopia Biosciences Inc. High throughput method for discovery of gene clusters
WO2002031155A2 (en) * 2000-10-13 2002-04-18 Ecopia Biosciences Inc. Gene cluster for ramoplanin biosynthesis
WO2002059322A2 (en) * 2000-10-17 2002-08-01 Cubist Pharmaceuticlas, Inc. Compositions and methods relating to the daptomycin biosynthetic gene cluster

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1337758C (en) * 1988-04-11 1995-12-19 Eli Lilly And Company Peptide antibiotics
WO2001053533A2 (en) * 2000-01-21 2001-07-26 Kosan Biosciences, Inc. Method for cloning polyketide synthase genes

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000020573A2 (en) * 1998-10-07 2000-04-13 Maxygen, Inc. Dna shuffling to produce nucleic acids for mycotoxin detoxification
WO2000040704A1 (en) * 1999-01-06 2000-07-13 The Regents Of The University Of California Bleomycin gene cluster components and their uses
WO2001030985A1 (en) * 1999-10-22 2001-05-03 Marahiel Mohamed A Tailored peptide synthetases, a method for their production and the use thereof
WO2002031155A2 (en) * 2000-10-13 2002-04-18 Ecopia Biosciences Inc. Gene cluster for ramoplanin biosynthesis
WO2002059322A2 (en) * 2000-10-17 2002-08-01 Cubist Pharmaceuticlas, Inc. Compositions and methods relating to the daptomycin biosynthetic gene cluster
CA2352451A1 (en) * 2001-07-24 2001-10-28 Ecopia Biosciences Inc. High throughput method for discovery of gene clusters

Non-Patent Citations (11)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL [online] 1 May 1999 (1999-05-01), XP002239871, accession no. EBI Database accession no. Q9Z4X6 *
DATABASE EMBL [online] 25 April 1997 (1997-04-25), DE CRECY-LAGARD V.A. ET AL., XP002239872, accession no. EBI Database accession no. Y11548 *
DATABASE SWALL [online] 1 December 2001 (2001-12-01), XP002254223, accession no. EBI Database accession no. Q93HG9 *
DATABASE SWALL [online] 1 December 2001 (2001-12-01), XP002254224, accession no. EBI Database accession no. Q93HB6 *
DATABASE SWALL [online] 1 December 2001 (2001-12-01), XP002254225, accession no. EBI Database accession no. Q93H11 *
DATABASE TREMBL [online] 1 June 1998 (1998-06-01), XP002254226, accession no. EBI Database accession no. O54155 *
DOEKEL S ET AL: "Biosynthesis of natural products on modular peptide synthetases", METABOLIC ENGINEERING, ACADEMIC PRESS,, US, vol. 3, no. 1, January 2001 (2001-01-01), pages 64 - 77, XP002237795, ISSN: 1096-7176 *
KONZ D ET AL: "MOLECULAR AND BIOCHEMICAL CHARACTERIZATION OF THE PROTEIN TEMPLATE CONTROLLING BIOSYNTHESIS OF THE LIPOPEPTIDE LICHENYSIN", JOURNAL OF BACTERIOLOGY, WASHINGTON, DC, US, vol. 181, no. 1, January 1999 (1999-01-01), pages 133 - 140, XP001113172, ISSN: 0021-9193 *
MCHENNEY MARGARET A ET AL: "Molecular cloning and physical mapping of the daptomycin gene cluster from Streptomyces roseosporus.", JOURNAL OF BACTERIOLOGY, vol. 180, no. 1, January 1998 (1998-01-01), pages 143 - 151, XP002239870, ISSN: 0021-9193 *
OMURA SATOSHI ET AL: "Genome sequence of an industrial microorganism Streptomyces avermitilis: Deducing the ability of producing secondary metabolites.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 98, no. 21, Q93HG9, 9 October 2001 (2001-10-09), October 9, 2001, pages 12215 - 12220, XP002254222, ISSN: 0027-8424 *
SYMMANK H ET AL: "Analysis of engineered multifunctional peptide synthetases. Enzymatic characterization of surfactin synthetase domains in hybrid bimodular systems", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, US, vol. 274, no. 31, 30 July 1999 (1999-07-30), pages 21581 - 21588, XP002133487, ISSN: 0021-9258 *

Also Published As

Publication number Publication date
WO2003060128A2 (en) 2003-07-24
JP2005514067A (en) 2005-05-19
CA2412226A1 (en) 2003-06-22
AU2002351637A1 (en) 2003-07-30
AU2002351636A1 (en) 2003-07-30
WO2003060127A2 (en) 2003-07-24
EP1458868A2 (en) 2004-09-22
EP1461434A2 (en) 2004-09-29
WO2003060127A3 (en) 2004-04-29

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