WO2003046163A3 - Multifunctional caffeic acid o-methyltransferase - Google Patents

Multifunctional caffeic acid o-methyltransferase Download PDF

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Publication number
WO2003046163A3
WO2003046163A3 PCT/EP2002/013320 EP0213320W WO03046163A3 WO 2003046163 A3 WO2003046163 A3 WO 2003046163A3 EP 0213320 W EP0213320 W EP 0213320W WO 03046163 A3 WO03046163 A3 WO 03046163A3
Authority
WO
WIPO (PCT)
Prior art keywords
seq
ring system
polypeptide
ortho
nucleic acid
Prior art date
Application number
PCT/EP2002/013320
Other languages
French (fr)
Other versions
WO2003046163A2 (en
Inventor
Wilfried Schwab
Ralf Kaldenhoff
Martina Wein
Original Assignee
Bayerische Julius Maximilians
Wilfried Schwab
Ralf Kaldenhoff
Martina Wein
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayerische Julius Maximilians, Wilfried Schwab, Ralf Kaldenhoff, Martina Wein filed Critical Bayerische Julius Maximilians
Priority to AU2002356735A priority Critical patent/AU2002356735A1/en
Publication of WO2003046163A2 publication Critical patent/WO2003046163A2/en
Publication of WO2003046163A3 publication Critical patent/WO2003046163A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The present invention is related to an isolated polypeptide capable of methylating an ortho-dihydroxy substituted ring system, whereby the ring system preferably is an aromatic, or heteroaromatic ring system or furanone based ring, and mimetics of said ortho-dihydroxy substituted ring system, selected from the group consisting of: (a) a polypeptide having an amino acid sequence which has at least 65% identity with amino acids 1 to 365 for the mature polypeptide of SEQ ID NO. 2; (b) a polypeptide which is encoded by a nucleic acid sequence which hybridizes under high stringency conditions with (i) the open reading frame coding for SEQ ID No 2 within the nucleotide sequence SEQ ID No. 1, (ii) a subsequence of (i) of at least 100 nucleotides, preferably of at least 100 consecutive nucleotides, or (iii) a complementary strand of (i), or (ii); (c) a polypeptide which is encoded by a nucleic acid having a nucleic acid sequence according to SEQ. ID. No. 1 or SEQ. ID. No. 3; (d) an allelic variant of (a), (b), or (c) and (e) a fragment of (a), (b), (c) or (d) that has methyltransferase activity using as a substrate an ortho-dihydroxy substituted ring system or a mimetic thereof.
PCT/EP2002/013320 2001-11-26 2002-11-26 Multifunctional caffeic acid o-methyltransferase WO2003046163A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002356735A AU2002356735A1 (en) 2001-11-26 2002-11-26 Multifunctional caffeic acid o-methyltransferase

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US33253401P 2001-11-26 2001-11-26
US60/332,534 2001-11-26

Publications (2)

Publication Number Publication Date
WO2003046163A2 WO2003046163A2 (en) 2003-06-05
WO2003046163A3 true WO2003046163A3 (en) 2003-08-28

Family

ID=23298651

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/013320 WO2003046163A2 (en) 2001-11-26 2002-11-26 Multifunctional caffeic acid o-methyltransferase

Country Status (2)

Country Link
AU (1) AU2002356735A1 (en)
WO (1) WO2003046163A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009511062A (en) * 2005-10-14 2009-03-19 コーネル ユニヴァーシティー Polynucleotides encoding enzymes of the lignin biosynthetic pathway in coffee
EP2757871A4 (en) * 2011-09-20 2015-05-20 Univ Florida Tomato catechol-o-methyltransferase sequences and methods of use
CN108169481B (en) * 2017-07-27 2023-07-07 河北省中药材质量检验检测研究中心有限公司 Test strip for detecting paeoniflorin and preparation method and application thereof
CN109867714A (en) * 2017-12-04 2019-06-11 陈勇 A kind of algae toxin and its method for extraction and purification
CN110243959A (en) * 2019-06-11 2019-09-17 华中农业大学 A kind of separation of endogenous fragrance component in Carnation flowers and identification method

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DATABASE EBI [online] XP002239054, retrieved from EMBL Database accession no. AF220491 *
ROSCHER R. ET AL.: "metabolism of 2,5-dimethyl-4-hydroxy-3(2h)-furanone in detached ripening strawberry fruits", J. AGRIC. FOODCHEM., vol. 45, 1997, pages 3202 - 3205, XP002239053 *

Also Published As

Publication number Publication date
AU2002356735A1 (en) 2003-06-10
AU2002356735A8 (en) 2003-06-10
WO2003046163A2 (en) 2003-06-05

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