WO2003022858A3 - Verfahren zum screenen von inhibitoren für die protein-protein-wechselwirkung sowie ribozyme hierzu - Google Patents

Verfahren zum screenen von inhibitoren für die protein-protein-wechselwirkung sowie ribozyme hierzu Download PDF

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Publication number
WO2003022858A3
WO2003022858A3 PCT/EP2002/010140 EP0210140W WO03022858A3 WO 2003022858 A3 WO2003022858 A3 WO 2003022858A3 EP 0210140 W EP0210140 W EP 0210140W WO 03022858 A3 WO03022858 A3 WO 03022858A3
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WO
WIPO (PCT)
Prior art keywords
domain
protein
inhibitors
screening
hairpin ribozyme
Prior art date
Application number
PCT/EP2002/010140
Other languages
English (en)
French (fr)
Other versions
WO2003022858A2 (de
Inventor
Seyed Hani Najafi-Shoushtari
Joerg Hartig
Michael Famulok
Original Assignee
Nascacell Gmbh
Seyed Hani Najafi-Shoushtari
Joerg Hartig
Michael Famulok
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE10144647A external-priority patent/DE10144647A1/de
Application filed by Nascacell Gmbh, Seyed Hani Najafi-Shoushtari, Joerg Hartig, Michael Famulok filed Critical Nascacell Gmbh
Priority to AU2002342672A priority Critical patent/AU2002342672A1/en
Publication of WO2003022858A2 publication Critical patent/WO2003022858A2/de
Publication of WO2003022858A3 publication Critical patent/WO2003022858A3/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C12N2310/122—Hairpin

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Die vorliegende Erfindung betrifft in einem Aspekt ein allosterisch regulierbares Hairpin-Ribozym. ln einem zweiten Aspekt betrifft die Erfindung ein Polynukleotid umfassend ein Hairpin-Ribozym, wobei das Hairpin-Ribozym mindestens eine substratbindende Domäne A und eine Domäne B umfasst, wobei bei Kontakt der Domäne B mit der Domäne A die katalytische Aktivität begründet wird und zwischen Domäne A und Domäne B eine Domäne C angeordnet ist, wobei die Domäne C die Domänen A und B verbindet und eine ein Zielmolekül bindende Nukleinsäure umfasst. ln einem dritten Aspekt betrifft die Erfindung ein Polynukleotid umfassend ein Hairpin-Ribozym, wobei das Hairpin-Ribozym mindestens eine substratbindende Domäne A und eine katalytische Domäne B umfasst, wobei bei Kontakt der Domäne B mit der Domäne A die katalytische Aktivität begründet wird, wobei zwischen Domäne A und Domäne B eine Domäne C angeordnet ist und die Domäne C die Domänen A und B verbindet und eine zu einer ein Zielmolekül bindenden Nukleinsäure zumindest teilweise komplementäre Nukleinsäure umfasst.
PCT/EP2002/010140 2001-09-11 2002-09-10 Verfahren zum screenen von inhibitoren für die protein-protein-wechselwirkung sowie ribozyme hierzu WO2003022858A2 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002342672A AU2002342672A1 (en) 2001-09-11 2002-09-10 Method for screening for inhibitors of protein/protein interaction and corresponding ribozymes

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE10144647A DE10144647A1 (de) 2001-09-11 2001-09-11 Verfahren zum Screenen von Inhibitoren für die Wechselwirkung zwischen Nukleinsäuren und Interaktoren sowie Robozyme hierzu
DE10144647.0 2001-09-11
DE10229393 2002-06-29
DE10229393.7 2002-06-29

Publications (2)

Publication Number Publication Date
WO2003022858A2 WO2003022858A2 (de) 2003-03-20
WO2003022858A3 true WO2003022858A3 (de) 2003-08-28

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/010140 WO2003022858A2 (de) 2001-09-11 2002-09-10 Verfahren zum screenen von inhibitoren für die protein-protein-wechselwirkung sowie ribozyme hierzu

Country Status (2)

Country Link
AU (1) AU2002342672A1 (de)
WO (1) WO2003022858A2 (de)

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* Cited by examiner, † Cited by third party
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US7115396B2 (en) 1998-12-10 2006-10-03 Compound Therapeutics, Inc. Protein scaffolds for antibody mimics and other binding proteins
US20040081647A1 (en) * 2002-08-27 2004-04-29 Afeyan Noubar B. Adzymes and uses thereof
BRPI0417302A (pt) 2003-12-05 2007-03-06 Compound Therapeutics Inc inibidores de receptores de fator de crescimento endotelial vascular do tipo 2
AU2007325838B2 (en) 2006-11-22 2013-09-19 Bristol-Myers Squibb Company Targeted therapeutics based on engineered proteins for tyrosine kinases receptors, including IGF-IR
EP2247615B1 (de) 2008-02-14 2014-07-30 Bristol-Myers Squibb Company Zielgerichtete therapeutika auf der basis von egfr gentechnisch hergestellten proteinen
JP2011520961A (ja) 2008-05-22 2011-07-21 ブリストル−マイヤーズ スクイブ カンパニー 多価フィブロネクチンをベースとする足場ドメインタンパク質
TWI496582B (zh) 2008-11-24 2015-08-21 必治妥美雅史谷比公司 雙重專一性之ｅｇｆｒ／ｉｇｆｉｒ結合分子
TW201138808A (en) 2010-05-03 2011-11-16 Bristol Myers Squibb Co Serum albumin binding molecules
WO2011150133A2 (en) 2010-05-26 2011-12-01 Bristol-Myers Squibb Company Fibronectin based scaffold proteins having improved stability
CN113150117A (zh) 2014-03-20 2021-07-23 百时美施贵宝公司 新的结合血清白蛋白的纤连蛋白iii型结构域
ES2781207T3 (es) 2015-09-23 2020-08-31 Bristol Myers Squibb Co Dominios de fibronectina de tipo iii de unión a seroalbúmina con velocidad de disociación rápida

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0277096A2 (de) * 1987-01-28 1988-08-03 Warner-Lambert Company Thrombin-Zubereitungen
US4959212A (en) * 1988-06-22 1990-09-25 Alexandra Stancesco Oxidizing-energizing composition and method for the treatment of diabetes
EP0468327A2 (de) * 1990-07-23 1992-01-29 Iketon Farmaceutici S.R.L. Hirudin enthaltende orale Arzneimittel
US5900407A (en) * 1997-02-06 1999-05-04 Inspire Pharmaceuticals, Inc. Method of treating dry eye disease with uridine triphosphates and related compounds
WO2002042491A2 (de) * 2000-11-22 2002-05-30 Nascacell Gmbh Verfahren zur identifizierung von verbindungen oder leitstrukturen gegen rna-target-motive und rna/protein wechselwirkungen

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0277096A2 (de) * 1987-01-28 1988-08-03 Warner-Lambert Company Thrombin-Zubereitungen
US4959212A (en) * 1988-06-22 1990-09-25 Alexandra Stancesco Oxidizing-energizing composition and method for the treatment of diabetes
EP0468327A2 (de) * 1990-07-23 1992-01-29 Iketon Farmaceutici S.R.L. Hirudin enthaltende orale Arzneimittel
US5900407A (en) * 1997-02-06 1999-05-04 Inspire Pharmaceuticals, Inc. Method of treating dry eye disease with uridine triphosphates and related compounds
WO2002042491A2 (de) * 2000-11-22 2002-05-30 Nascacell Gmbh Verfahren zur identifizierung von verbindungen oder leitstrukturen gegen rna-target-motive und rna/protein wechselwirkungen

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
ESTEBAN ET AL.: "Structural basis for heterogeneous kinetics: Reengineering the hairpin ribozyme", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCE US, vol. 95, May 1998 (1998-05-01), pages 6091 - 6096, XP002234998 *
FAMULOK M: "OLIGONUCLEOTIDE APTAMERS THAT RECOGNIZE SMALL MOLECULES", CURRENT OPINION IN STRUCTURAL BIOLOGY, CURRENT BIOLOGY LTD., LONDON, GB, vol. 9, no. 3, 1999, pages 324 - 329, XP001031183, ISSN: 0959-440X *
KOMATSU ET AL.: "In vitro selection of hairpin ribozymes activated with short oligonucleotides", BIOCHEMISTRY, vol. 41, 23 July 2002 (2002-07-23), pages 9090 - 9098, XP002235000 *
ROBERTSON MICHAEL P ET AL: "Design and optimization of effector-activated ribozyme ligases", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 28, no. 8, 15 April 2000 (2000-04-15), pages 1751 - 1759, XP002205328, ISSN: 0305-1048 *
SOUKUP GARRETT A ET AL: "Engineering precision RNA molecular switches", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 96, no. 7, 30 March 1999 (1999-03-30), pages 3584 - 3589, XP002158536, ISSN: 0027-8424 *
WALTER ET AL.: "Stability of hairpin ribozyme tertiary structure is governed by the interdomain junction", NATURE STRUCTURAL BIOLOGY, vol. 6, no. 6, 1999, pages 544 - 549, XP002234999 *

Also Published As

Publication number Publication date
AU2002342672A1 (en) 2003-03-24
WO2003022858A2 (de) 2003-03-20

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