WO2003019172A3 - Apparatus and method for separating an analyte - Google Patents

Apparatus and method for separating an analyte Download PDF

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Publication number
WO2003019172A3
WO2003019172A3 PCT/EP2002/010369 EP0210369W WO03019172A3 WO 2003019172 A3 WO2003019172 A3 WO 2003019172A3 EP 0210369 W EP0210369 W EP 0210369W WO 03019172 A3 WO03019172 A3 WO 03019172A3
Authority
WO
WIPO (PCT)
Prior art keywords
compartments
compounds
analyte solution
analyte
solution
Prior art date
Application number
PCT/EP2002/010369
Other languages
French (fr)
Other versions
WO2003019172A2 (en
Inventor
Philippe Michel
Frederic Reymond
Joel Stephane Rossier
Original Assignee
Diagnoswiss Sa
Philippe Michel
Frederic Reymond
Joel Stephane Rossier
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Diagnoswiss Sa, Philippe Michel, Frederic Reymond, Joel Stephane Rossier filed Critical Diagnoswiss Sa
Priority to DE60206937T priority Critical patent/DE60206937T2/en
Priority to JP2003523988A priority patent/JP4227016B2/en
Priority to US10/486,181 priority patent/US7390389B2/en
Priority to EP02772306A priority patent/EP1421373B1/en
Priority to AT02772306T priority patent/ATE308042T1/en
Publication of WO2003019172A2 publication Critical patent/WO2003019172A2/en
Publication of WO2003019172A3 publication Critical patent/WO2003019172A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44795—Isoelectric focusing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Electrochemistry (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Electrostatic Separation (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The present invention relates to an apparatus and method for the separation and purification of charged and neutral compounds in an analyte solution by electrophoretic means using a plurality of compartments (1) to contain the analyte solution, an immobilized chemical buffering system (2) to fix a pH value or a pH gradient in its portion(s) contacting the analyte solution and an electrical field imposed by electrical means (4) disposed in at least two of said compartments. This invention allows one to differentially separate charged and neutral compounds, to extract the migrating compounds from the chemical buffering system (2), to recover the purified compounds mainly in solution and to collect them in the various compartments (4), preferably at various pI.
PCT/EP2002/010369 2001-08-31 2002-08-30 Apparatus and method for separating an analyte WO2003019172A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
DE60206937T DE60206937T2 (en) 2001-08-31 2002-08-30 DEVICE AND METHOD FOR SEPARATING ANALYTES
JP2003523988A JP4227016B2 (en) 2001-08-31 2002-08-30 Method and apparatus for separating analytes
US10/486,181 US7390389B2 (en) 2001-08-31 2002-08-30 Apparatus and method for separating an analyte
EP02772306A EP1421373B1 (en) 2001-08-31 2002-08-30 Apparatus and method for separating an analyte
AT02772306T ATE308042T1 (en) 2001-08-31 2002-08-30 APPARATUS AND METHOD FOR THE SEPARATION OF ANALYTES

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0121189.5A GB0121189D0 (en) 2001-08-31 2001-08-31 Apparatus and method for separating an analyte
GB0121189.5 2001-08-31

Publications (2)

Publication Number Publication Date
WO2003019172A2 WO2003019172A2 (en) 2003-03-06
WO2003019172A3 true WO2003019172A3 (en) 2003-12-18

Family

ID=9921346

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/010369 WO2003019172A2 (en) 2001-08-31 2002-08-30 Apparatus and method for separating an analyte

Country Status (7)

Country Link
US (1) US7390389B2 (en)
EP (1) EP1421373B1 (en)
JP (1) JP4227016B2 (en)
AT (1) ATE308042T1 (en)
DE (1) DE60206937T2 (en)
GB (1) GB0121189D0 (en)
WO (1) WO2003019172A2 (en)

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7105810B2 (en) 2001-12-21 2006-09-12 Cornell Research Foundation, Inc. Electrospray emitter for microfluidic channel
GB0226160D0 (en) * 2002-11-08 2002-12-18 Diagnoswiss Sa Apparatus for dispensing a sample in electrospray mass spectrometers
GB0300820D0 (en) * 2003-01-14 2003-02-12 Diagnoswiss Sa Membrane-microchannel strip
EP1673595A2 (en) * 2003-09-15 2006-06-28 DiagnoSwiss S.A. Microfluidic flow monitoring device
WO2005029061A1 (en) * 2003-09-24 2005-03-31 Agilent Technologies, Inc. Extraction of molecules using frame
US7537807B2 (en) 2003-09-26 2009-05-26 Cornell University Scanned source oriented nanofiber formation
US8529744B2 (en) 2004-02-02 2013-09-10 Boreal Genomics Corp. Enrichment of nucleic acid targets
US10337054B2 (en) 2004-02-02 2019-07-02 Quantum-Si Incorporated Enrichment of nucleic acid targets
WO2005072854A1 (en) 2004-02-02 2005-08-11 The University Of British Columbia Scodaphoresis and methods and apparatus for moving and concentrating particles
US20070240986A1 (en) * 2004-11-12 2007-10-18 Diagnoswiss S.A. Microfluidic Device with Minimized Ohmic Resistance
WO2006063625A1 (en) * 2004-12-17 2006-06-22 Agilent Technologies, Inc. Fractionation using electro elution
CA2496294A1 (en) 2005-02-07 2006-08-07 The University Of British Columbia Apparatus and methods for concentrating and separating particles such as molecules
EP1669751B1 (en) * 2005-07-07 2007-12-12 Agilent Technologies, Inc. Modular system for gel electrophoresis device
WO2007051492A1 (en) 2005-11-02 2007-05-10 Agilent Technologies, Inc. Force-promoted sample recovery in gel electrophoresis
GB0607205D0 (en) * 2006-04-10 2006-05-17 Diagnoswiss Sa Miniaturised biosensor with optimized anperimetric detection
US20090178929A1 (en) * 2006-06-02 2009-07-16 Koninklijke Philips Electronics N.V. Separation device for isoelectric focusing
EP2559666B1 (en) * 2007-08-27 2014-07-23 Technion Research & Development Foundation Ltd. pH gradients controlled by electrolysis, and their use in isoelectric focusing
EP2238434A4 (en) 2008-02-01 2011-06-22 Univ British Columbia Methods and apparatus for particle introduction and recovery
EP2146200A1 (en) 2008-07-16 2010-01-20 Agilent Technologies, Inc. Device and method for isoelectric focusing
US8877028B2 (en) 2009-04-21 2014-11-04 The University Of British Columbia System and methods for detection of particles
CN101556261B (en) * 2009-05-25 2012-01-11 北京理工大学 Method for detecting microorganism isoelectric point by adopting microtubule isoelectric focusing and cutting-blowout culture
US9028664B2 (en) * 2009-08-18 2015-05-12 Technion Research & Development Foundation Limited Proton concentration topographies, methods and devices for producing the same
US8864970B2 (en) 2009-08-18 2014-10-21 Technion Research & Development Foundation Limited Methods and devices of separating molecular analytes
WO2013134742A2 (en) 2012-03-08 2013-09-12 Cyvek, Inc Micro-tube particles for microfluidic assays and methods of manufacture
US9855735B2 (en) 2009-11-23 2018-01-02 Cyvek, Inc. Portable microfluidic assay devices and methods of manufacture and use
US10022696B2 (en) 2009-11-23 2018-07-17 Cyvek, Inc. Microfluidic assay systems employing micro-particles and methods of manufacture
US9759718B2 (en) 2009-11-23 2017-09-12 Cyvek, Inc. PDMS membrane-confined nucleic acid and antibody/antigen-functionalized microlength tube capture elements, and systems employing them, and methods of their use
US9700889B2 (en) * 2009-11-23 2017-07-11 Cyvek, Inc. Methods and systems for manufacture of microarray assay systems, conducting microfluidic assays, and monitoring and scanning to obtain microfluidic assay results
US10065403B2 (en) 2009-11-23 2018-09-04 Cyvek, Inc. Microfluidic assay assemblies and methods of manufacture
US9500645B2 (en) 2009-11-23 2016-11-22 Cyvek, Inc. Micro-tube particles for microfluidic assays and methods of manufacture
CN106552682B (en) 2011-03-22 2020-06-19 西维克公司 Microfluidic device and methods of manufacture and use
CA2742327A1 (en) 2011-05-20 2012-11-20 The University Of British Columiba Systems and methods for enhanced scoda
WO2013067509A1 (en) 2011-11-04 2013-05-10 Bio-Rad Laboratories, Inc. Simultaneous purification of cell components
CN104024856B (en) * 2011-11-04 2018-10-26 生物辐射实验室股份有限公司 Protein fractionation based on pI
US9766207B2 (en) 2011-11-04 2017-09-19 Bio-Rad Laboratories, Inc. Affinity methods and compositions employing electronic control of pH
WO2013104994A2 (en) 2012-01-13 2013-07-18 The University Of British Columbia Multiple arm apparatus and methods for separation of particles
WO2013123425A1 (en) 2012-02-15 2013-08-22 Bio-Rad Laboratories, Inc. Electronic control of ph and ionic strength
US9321012B2 (en) 2012-04-04 2016-04-26 Bio-Rad Laboratories, Inc. Electronic protein fractionation
WO2013166444A2 (en) 2012-05-04 2013-11-07 Boreal Genomics Corp. Biomarker analysis using scodaphoresis
US9340835B2 (en) 2013-03-15 2016-05-17 Boreal Genomics Corp. Method for separating homoduplexed and heteroduplexed nucleic acids
US11130986B2 (en) 2015-05-20 2021-09-28 Quantum-Si Incorporated Method for isolating target nucleic acid using heteroduplex binding proteins
CN106582289B (en) * 2015-10-16 2021-02-05 中美华世通生物医药科技（武汉）有限公司 Filtration system and filtration method
US10228367B2 (en) 2015-12-01 2019-03-12 ProteinSimple Segmented multi-use automated assay cartridge
CN107245093A (en) * 2017-08-09 2017-10-13 赖兵 A kind of electricity purification devices such as protein and method
CA3159566A1 (en) 2019-10-29 2021-05-06 Quantum-Si Incorporated Peristaltic pumping of fluids and associated methods, systems, and devices

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000074850A2 (en) * 1999-06-03 2000-12-14 University Of Washington Microfluidic devices for transverse electrophoresis and isoelectric focusing
WO2001068225A1 (en) * 2000-03-15 2001-09-20 Proteosys Ag Micropreparative isoelectric focussing
WO2001075432A2 (en) * 2000-04-03 2001-10-11 The Wistar Institute A method and device for analysis of charged molecules by solution isoelectric focusing
WO2001086279A1 (en) * 2000-05-05 2001-11-15 Ecole Polytechnique Federale De Lausanne Electrophoretic separation of compounds

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2815320A (en) 1953-10-23 1957-12-03 Kollsman Paul Method of and apparatus for treating ionic fluids by dialysis
DE2051715B2 (en) 1970-10-21 1976-01-15 SEPARATION CHAMBER FOR CONTINUOUS DEFLECTION ELECTROPHORESIS
US3719580A (en) 1971-06-04 1973-03-06 R Roberts Electrophoretic apparatus
GB1422118A (en) 1972-01-04 1976-01-21 Bovril Ltd Vertical gel electrophoresis apparatus
US3888758A (en) 1973-06-28 1975-06-10 Sheik Arshad Saeed Apparatus for large scale gel electrophoresis
US4148703A (en) 1976-02-11 1979-04-10 Morton Weintraub Method of electrophoretic purification of enzymes and peptides by means of an adjustable, specialized, geometrically located electrode system
US4362612A (en) 1978-04-18 1982-12-07 University Patents, Inc. Isoelectric focusing apparatus
CA1238296A (en) 1984-08-02 1988-06-21 Claude Hamelin Electrophoresis system for multiple agarose slab gels
EP0287513B1 (en) 1987-04-11 1992-12-02 Ciba-Geigy Ag An isoelectric focusing process and a means for carrying out said process
US5114555A (en) 1988-01-05 1992-05-19 Monsanto Company Continuous isoelectric separation
US5126022A (en) * 1990-02-28 1992-06-30 Soane Tecnologies, Inc. Method and device for moving molecules by the application of a plurality of electrical fields
US5104512A (en) 1990-05-14 1992-04-14 Labintelligence, Inc. Gel electrophoresis system
US5149418A (en) 1991-09-04 1992-09-22 Apogee Designs, Ltd. Vertical gel electrophoresis apparatus having universal gel assembly support structure
NO315930B1 (en) 1995-01-18 2003-11-17 Picower Inst For Medical Res T Use of Thiazolium Compounds in the Preparation of Pharmaceutical Preparations, Compounds Containing the Compounds, and Nyetiazolium Compounds
IT1272932B (en) * 1995-01-24 1997-07-01 Pier Giorgio Righetti IMMOBILIZED ENZYME REACTOR
US5540826A (en) 1995-03-15 1996-07-30 Protein Technologies, Inc. Multi-channel separation device
CA2192262C (en) 1995-12-08 2011-03-15 Yoshihide Hayashizaki Method for purification and transfer to separation/detection systems of dna sequencing samples and plates used therefor
US5773645A (en) 1997-05-05 1998-06-30 Bio-Rad Laboratories, Inc. Two-dimensional electrophoresis device
US6013165A (en) 1998-05-22 2000-01-11 Lynx Therapeutics, Inc. Electrophoresis apparatus and method
AUPP576598A0 (en) * 1998-09-07 1998-10-01 Life Therapeutics Limited Cassette for macromolecule purification
US6706162B1 (en) 2000-09-25 2004-03-16 Applera Corporation High speed, high resolution compositions, methods, and kits for capillary electrophoresis

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000074850A2 (en) * 1999-06-03 2000-12-14 University Of Washington Microfluidic devices for transverse electrophoresis and isoelectric focusing
WO2001068225A1 (en) * 2000-03-15 2001-09-20 Proteosys Ag Micropreparative isoelectric focussing
WO2001075432A2 (en) * 2000-04-03 2001-10-11 The Wistar Institute A method and device for analysis of charged molecules by solution isoelectric focusing
WO2001086279A1 (en) * 2000-05-05 2001-11-15 Ecole Polytechnique Federale De Lausanne Electrophoretic separation of compounds

Also Published As

Publication number Publication date
US20040231986A1 (en) 2004-11-25
WO2003019172A2 (en) 2003-03-06
DE60206937D1 (en) 2005-12-01
US7390389B2 (en) 2008-06-24
ATE308042T1 (en) 2005-11-15
EP1421373A2 (en) 2004-05-26
EP1421373B1 (en) 2005-10-26
JP4227016B2 (en) 2009-02-18
GB0121189D0 (en) 2001-10-24
DE60206937T2 (en) 2006-07-27
JP2005502864A (en) 2005-01-27

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