CN106582289B - Filtration system and filtration method - Google Patents
Filtration system and filtration method Download PDFInfo
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- CN106582289B CN106582289B CN201510677054.6A CN201510677054A CN106582289B CN 106582289 B CN106582289 B CN 106582289B CN 201510677054 A CN201510677054 A CN 201510677054A CN 106582289 B CN106582289 B CN 106582289B
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Abstract
The invention discloses a filtering system and a filtering method, wherein the filtering system comprises: a sample tray having at least one placement aperture adapted to place a chromatography vial; the device comprises a filtration module, a liquid inlet and a gas inlet, wherein the filtration module comprises a first pore plate and a second pore plate which are attached to each other, at least one filtration membrane is fixed between the first pore plate and the second pore plate, a volume tube is arranged on the first pore plate at a position corresponding to a chromatogram sampling bottle, and the volume tube is provided with the liquid inlet and the gas inlet; the second orifice plate is provided with the filter orifice in the position department that corresponds chromatogram appearance bottle of advancing, the filter orifice with chromatogram appearance bottle intercommunication advances, wherein the equal upper and lower correspondence in position of volume pipe, filter membrane, filter orifice and chromatogram appearance bottle of advancing. This filtration system is suitable for and uses with the supporting of automatic sampling system's chromatogram appearance bottle, from this, utilizes this filtration system, can conveniently realize treating the filtration of filtering the sample fast to through handling chromatogram appearance bottle in batches, can realize the batch of a plurality of samples, efficient filtration and shift, the operation is very convenient, and can guarantee filtration quality.
Description
Technical Field
The invention belongs to the field of instrument analysis, and particularly relates to a filtering system and a filtering method.
Background
At present, with the improvement of the automation level of instruments, an automatic sample introduction system is more and more widely applied to the instrument analysis industry, and the efficiency of batch sample treatment is greatly improved through batch chromatographic sample introduction bottles. Each sample needs to be filtered through a filter before being transferred to a chromatography injection bottle. In the prior art, needle filters are commonly used, where a medical syringe is first used to draw a liquid sample into the syringe needle, the needle filter is attached to the syringe head, the filtrate is squeezed and transferred to a chromatographic vial. The process needs to transfer samples twice, so that sample loss is easily caused, and the process is not suitable for sample preparation with small amount; but also consumes various consumables such as an injector and the like; the operation is only one sample after the other, and the efficiency is very low.
Thus, existing filtration systems and filtration techniques suitable for use with chromatography vials of autosampler systems are in need of further improvement.
Disclosure of Invention
The present invention aims to solve at least to some extent one of the technical problems of the prior art or at least to provide a useful commercial choice. Therefore, an object of the present invention is to provide a filtration system and a filtration method suitable for use with a chromatography sampling bottle of an autosampler system, by which samples can be efficiently filtered and transferred in batches, the operation is very convenient, and the filtration quality can be ensured.
To this end, in a first aspect of the invention, the invention proposes a filtration system. According to an embodiment of the invention, the system comprises:
a sample tray having at least one placement aperture adapted to place a chromatography vial;
the device comprises a filtration module, a liquid inlet and a gas inlet, wherein the filtration module comprises a first pore plate and a second pore plate which are attached to each other, at least one filtration membrane is fixed between the first pore plate and the second pore plate, a volume tube is arranged on the first pore plate at a position corresponding to a chromatogram sampling bottle, and the volume tube is provided with the liquid inlet and the gas inlet; the second orifice plate is provided with the filter orifice in the position department that corresponds chromatogram appearance bottle of advancing, the filter orifice with chromatogram appearance bottle intercommunication advances, wherein the equal upper and lower correspondence in position of volume pipe, filter membrane, filter orifice and chromatogram appearance bottle of advancing.
The inventor surprisingly finds that the filtering system is suitable for being matched with a chromatographic sample feeding bottle of an automatic sample feeding system, so that the filtering system can conveniently and quickly filter samples to be filtered, and synchronous filtering of a plurality of samples can be realized by batch processing of the chromatographic sample feeding bottles and the filtering templates corresponding to the sample feeding bottles through the filtering system, so that the sample processing efficiency is greatly improved.
According to some specific examples of the present invention, the sample tray has a plurality of placing holes, the plurality of chromatography vials are provided, and the plurality of chromatography vials are respectively provided in the plurality of placing holes in a one-to-one correspondence.
According to some embodiments of the invention, the filter port and the chromatography vial are in communication via a filter tube.
According to some specific examples of the invention, the lower end of the filter tube is inserted into the chromatography bottle, and the lower end has an inclined surface.
According to an embodiment of the present invention, the first orifice plate and the second orifice plate have a first partition assembly and a second partition assembly thereon, respectively, the first partition assembly and the second partition assembly cooperating to connect the first orifice plate and the second orifice plate. Thereby, the interconnection of the first orifice plate and the second orifice plate can be effectively achieved.
According to some embodiments of the invention, each of the first partition assembly and the second partition assembly includes a plurality of first direction partitions and a plurality of second direction partitions thereon, the plurality of first direction partitions and the plurality of second direction partitions collectively defining a plurality of cells therebetween. Therefore, the partition of different positions on the first pore plate and the second pore plate can be realized, and the blocked unit just corresponds to the position of the chromatographic sampling bottle, so that the mutual interference of different samples during filtering is avoided, and the cross contamination of the samples is also avoided.
According to an embodiment of the present invention, the number and the position of the plurality of cells on the first well plate and the second well plate, which are partitioned by the plurality of first direction partitions and the plurality of second direction partitions in common, correspond to the number and the position of the plurality of chromatography bottles, one for one.
According to some specific examples of the present invention, the plurality of first direction partitions are parallel to each other, the plurality of second direction partitions are parallel to each other, and an angle α between the first direction partitions and an extending direction of the second direction partitions satisfies 0 < α ≦ 90 degrees. Thereby, the first orifice plate and the second orifice plate can be attached to each other.
According to some specific examples of the present invention, the first direction spacer and the second direction spacer extend in directions perpendicular to each other, and the first orifice plate and the second orifice plate are divided into a plurality of square units by the plurality of first direction spacers and the plurality of second direction spacers, respectively; the side length of the square unit is larger than the diameter of the filter membrane. Therefore, the filter membrane can be conveniently fixed, the filter membranes in different square units can be blocked, on the other hand, the first pore plate and the second pore plate form a whole and are further correspondingly connected with the chromatographic sample injection bottle, and the filter module and the chromatographic sample injection bottle can be conveniently connected; and the edge portions of the filter membrane can be located within square cells.
According to some embodiments of the invention, the first partition assembly comprises a first rib protruding towards the second orifice plate and/or a first groove recessed away from the second orifice plate; the second partition assembly comprises a second groove matched with the first convex strip and/or a second convex strip matched with the first groove. Therefore, the first pore plate and the second pore plate can be tightly attached to each other, the filter membrane can be conveniently fixed, and the filter membranes in different units can be blocked and separated.
According to some embodiments of the invention, the gas inlet is connected to a gas booster pump. It is thereby possible to achieve that the sample to be treated passes the filter membrane under gas pressure.
According to an embodiment of the present invention, the filter membrane is an organic filter membrane or an aqueous filter membrane, and can be freely selected according to the characteristics of the sample to be treated. Thereby increasing the applicability of the filtration system of the present invention.
According to an embodiment of the invention, the filter membrane is a 0.45 micron microporous filter membrane. Therefore, the requirement of the high performance liquid chromatograph on filtering the sample to be processed can be met.
In some embodiments of the present invention, the liquid inlet may be further connected to a drainage tube, which is inserted into the sample to be treated, and absorbs the sample to be treated through the drainage tube for filtration.
In some embodiments of the present invention, the sample tray is made of a material having elasticity, such as a plastic plate, a rubber plate, or a silicone plate. The chromatographic sample introduction bottle is embedded in the placing hole. On the other hand, the sample tray can be moved into the ultrasonic wave as a whole, so that the chromatographic sampling bottles can be cleaned in batches.
In some embodiments of the present invention, the diameter of the sample bottle placing hole is slightly smaller than that of the sample bottle, so that the sample bottle is fixed by using the elasticity of the sample tray.
In some embodiments of the invention, the filtration module is removably coupled to the chromatography sample vial. Whereby, when filtering a sample, a filtration module is placed on the chromatography sample vial; and after the sample is filtered, the filtering module is detached and replaced by a conventional chromatographic sample injection bottle cap. Therefore, the chromatographic sample injection bottle containing the filtered sample can be directly placed in the high-efficiency liquid-phase automatic sample injector for detection.
In a second aspect of the invention, the invention provides a method of filtering a sample using the above-described filtration system. According to an embodiment of the invention, the method comprises the steps of:
(1) placing a chromatographic sample introduction bottle in the sample tray, wherein the bottle mouth of the chromatographic sample introduction bottle faces upwards;
(2) placing a filter membrane between the first and second well plates;
(3) aspirating a sample to be filtered through a liquid inlet of a volume tube on a first well plate;
(4) and pressing the sample to be filtered by using a gas pressurizing pump through a gas inlet of the volume tube on the first pore plate so that the sample to be filtered enters the chromatographic sampling bottle through the filter membrane.
The method is suitable for the treatment of the chromatographic sample injection bottle of the automatic sample injection system, so that the filtering method can conveniently and quickly realize the filtering of the sample to be filtered, and the filtering system can realize that batch samples are simultaneously filtered through the filter membrane and enter different chromatographic sample injection bottles so as to be conveniently butted with the subsequent high-efficiency liquid-phase automatic sample injection process in time.
Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.
Drawings
The above and/or additional aspects and advantages of the present invention will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings of which:
FIG. 1 is a schematic diagram of a filtration system according to one embodiment of the present invention;
FIG. 2 is a schematic diagram of a partial structure of a square unit in a filtration system according to one embodiment of the present invention;
FIG. 3 is a top view of a first direction spacer in a filtration system according to one embodiment of the present invention;
FIG. 4 is a top view of a second direction spacer in a filtration system according to one embodiment of the present invention.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are illustrative and intended to be illustrative of the invention and are not to be construed as limiting the invention.
In the description of the present invention, it is to be understood that the terms "central," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," "circumferential," and the like are used in the orientations and positional relationships indicated in the drawings for convenience in describing the invention and to simplify the description, and are not intended to indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and are not to be considered limiting of the invention.
Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.
In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
In the present invention, unless otherwise expressly stated or limited, the first feature "on" or "under" the second feature may be directly contacting the first and second features or indirectly contacting the first and second features through an intermediate. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.
In a first aspect of the invention, a filtration system is presented. The filtering system is suitable for being matched with a chromatographic sample feeding bottle of an automatic sample feeding system, so that the filtering system can conveniently and quickly filter samples to be filtered, and synchronous filtering of a plurality of samples can be realized by batch processing of the chromatographic sample feeding bottles and the filtering templates corresponding to each sample feeding bottle through the filtering system, so that the sample processing efficiency is greatly improved.
For ease of understanding, the filtration system of the present invention is described in detail below with reference to fig. 1-4.
According to an embodiment of the invention, the filtration system 1000 of the invention comprises: a sample tray 100 and a filtration module 200.
According to an embodiment of the present invention, the sample tray 100 has at least one placement hole 110 adapted to place a chromatography vial 120.
According to the embodiment of the invention, the filtration module 200 comprises a first pore plate 210 and a second pore plate 220 which are attached to each other, at least one filter membrane 240 is fixed between the first pore plate 210 and the second pore plate 220, the first pore plate 210 is provided with a volume tube 250 at a position corresponding to the chromatographic sample bottle 120, and the volume tube 250 is provided with a liquid inlet 251 and a gas inlet 252; the second hole plate 220 is provided with a filtering port 270 at a position corresponding to the chromatographic bottle 120, the filtering port 270 is communicated with the chromatographic bottle 120, and the positions of the volume tube 250, the filtering membrane 240, the filtering port 270 and the chromatographic bottle 120 are vertically corresponding.
Therefore, the filtering system of the chromatographic sample feeding bottle 120 can conveniently and quickly realize the filtering of samples to be filtered, and the chromatographic sample feeding bottles 120 and the filtering templates corresponding to the sample feeding bottles are processed in batches by the filtering system of the invention, so that the synchronous filtering of a plurality of samples can be realized, and the sample processing efficiency is greatly improved.
It should be noted that, as used herein, the expression "the positions of the volume tube 250, the filter membrane 240, the filter port 270, and the chromatography bottle 120 correspond up and down" means that the positions of the volume tube 250, the filter membrane 240, the filter port 270, and the chromatography bottle 120 correspond one to one in the axial direction of the chromatography bottle 120.
According to some specific examples of the present invention, the sample tray 100 has a plurality of placing holes 110, the plurality of chromatography sample vials 120 have a plurality of placing holes 110, and the plurality of chromatography sample vials 120 are respectively disposed in the plurality of placing holes 110 in a one-to-one correspondence.
According to some embodiments of the present invention, the filter port 270 communicates with the chromatography bottle 120 via a filter tube 260.
According to some embodiments of the invention, the lower end of the filter tube 260 is inserted into the chromatography bottle 120, and the lower end has an inclined surface.
According to an embodiment of the present invention, the first orifice plate 210 and the second orifice plate 220 have a first partition assembly 280 and a second partition assembly 290 thereon, respectively, the first partition assembly 280 and the second partition assembly 290 cooperating to connect the first orifice plate 210 and the second orifice plate 220. Thereby, the interconnection of the first orifice plate 210 and the second orifice plate 220 can be effectively achieved.
According to some embodiments of the present invention, each of the first partition assembly 280 and the second partition assembly 290 includes a plurality of first direction partitions 231 and a plurality of second direction partitions 232 thereon, and the plurality of first direction partitions 231 and the plurality of second direction partitions 232 partition a plurality of cells in common therebetween. Therefore, the separation of different positions on the first pore plate 210 and the second pore plate 220 can be realized, and the unit formed by the separation just corresponds to the position of the chromatographic sampling bottle 120, so that the mutual interference of different samples during filtering is avoided, and the cross contamination of the samples is also avoided.
Specifically, the first partition assembly 280 positioned on the first orifice plate 210 includes a plurality of first direction partitions 231-1 and a plurality of second direction partitions 232-1, the second partition assembly 290 positioned on the second orifice plate 220 includes a plurality of first direction partitions 231-2 and a plurality of second direction partitions 232-2, and the plurality of first direction partitions 231-1 on the first orifice plate 210 and the plurality of first direction partitions 231-2 on the second orifice plate 220 are engaged with each other, and the plurality of second direction partitions 232-1 on the first orifice plate 210 and the plurality of second direction partitions 232-2 on the second orifice plate 220 are engaged with each other, so as to connect the first orifice plate 210 and the second orifice plate 220 to each other and partition a plurality of units.
Wherein fig. 3 and 4 show top views of the first direction spacer 231 and the second direction spacer 232, respectively, according to one embodiment of the present invention. Further, according to the embodiment of the present invention, the number and the position of the plurality of cells on the first well plate 210 and the second well plate 220, which are partitioned by the plurality of first direction partitions 231 and the plurality of second direction partitions 232 in common, correspond to the number and the position of the plurality of chromatography sample vials 120, one for one.
According to some specific examples of the present invention, the plurality of first direction partitions 231 are parallel to each other, the plurality of second direction partitions 232 are parallel to each other, and an angle α between the first direction partitions 231 and the extending direction of the second direction partitions 232 satisfies 0 < α ≦ 90 degrees. Thereby, the first orifice plate 210 and the second orifice plate 220 can be closely attached to each other.
According to some specific examples of the present invention, the first direction spacer 231 and the second direction spacer 232 extend in directions perpendicular to each other, and the first orifice plate 210 and the second orifice plate 220 are divided into a plurality of square units 23 by the plurality of first direction spacers 231 and the plurality of second direction spacers 232, respectively; the sides of the square cells 23 are larger than the diameter of the filter membrane 240. Thereby, the filter membrane 240 is convenient to be fixed and the filter membranes 240 in different square units 23 are blocked, on the other hand, the first pore plate 210 and the second pore plate 220 are integrated and further correspondingly connected with the chromatographic sample bottle 120, so that the filter module 200 and the chromatographic sample bottle 120 are convenient to be connected; and enables the edge portions of the filter membrane 240 to be located within the square cells 23.
According to some embodiments of the invention, the first partition assembly 280 comprises a first rib protruding towards the second orifice plate 220 and/or a first groove recessed away from the second orifice plate 220; the second break assembly 290 comprises a second groove cooperating with the first rib and/or a second rib cooperating with the first groove. Thus, the first and second well plates 210 and 220 can be closely attached to each other, and the filter membrane 240 can be easily fixed and the filter membranes 240 in different units can be blocked.
It should be noted that, as used herein, the expression that the first partition assembly 280 includes a first convex strip protruding toward the second orifice plate 220 and/or a first concave groove recessed away from the second orifice plate 220 means that the first partition assembly 280 can have three conditions:
(1) the first partition assembly 280 includes only a first rib protruding toward the second orifice plate 220 (accordingly, the second partition assembly 290 includes only a second groove cooperating with the first rib);
(2) the first partition assembly 280 includes only first grooves recessed away from the second orifice plate 220 (correspondingly, the second partition assembly 290 includes only second ribs that mate with the first grooves); and
(3) the first partition assembly 280 includes both a first rib protruding toward the second orifice plate 220 and a first groove recessed away from the second orifice plate 220 (accordingly, the second partition assembly 290 includes both a second groove cooperating with the first rib and a second rib cooperating with the first groove).
According to an embodiment of the present invention, the liquid inlet 251 may be further connected with a drain tube 253, and the drain tube 253 is inserted into the sample 254 to be processed, and the sample 254 to be processed is absorbed through the drain tube 253 to be filtered.
According to some embodiments of the invention, the gas inlet 252 is connected to a gas pressurizing pump 255. Whereby the passage of the sample to be treated through the filter membrane 240 under gas pressure can be achieved.
According to the embodiment of the present invention, the filter 240 is an organic filter or an aqueous filter, which can be freely selected according to the characteristics of the sample to be processed. Thereby increasing the applicability of the filtration system of the present invention.
According to an embodiment of the invention, the filter membrane 240 is a 0.45 micron microporous filter membrane. Therefore, the requirement of the high performance liquid chromatograph on filtering the sample to be processed can be met.
In some embodiments of the present invention, the sample tray 100 is made of a material with elasticity, such as a plastic plate, a rubber plate, or a silicone plate. The chromatographic sample bottle 120 is fitted in the placing hole 110. On the other hand, the sample tray 100 may be moved into the ultrasonic wave as a whole to clean the chromatography bottles in batches.
In some embodiments of the present invention, the diameter of the vial placement hole 110 is slightly smaller than that of the vial, so that the vial is fixed by the elasticity of the sample tray 100 itself.
In some embodiments of the invention, the filtration module 200 is removably coupled to the chromatography sample vial 120. Thus, when filtering a sample, the filtration module 200 is placed on the chromatography vial 120; when the sample filtration is completed, the filtration module 200 is removed and replaced with a conventional cap of a chromatography sample injection bottle. Thus, the filtered sample vial 120 may be placed directly into the hplc autosampler for detection.
In a second aspect of the invention, a method of filtering a sample is provided. According to an embodiment of the present invention, the method is performed using the filtration system 1000 described above. According to an embodiment of the invention, the method comprises the steps of:
(1) placing a chromatography sampling bottle 120 in the sample tray 100, wherein the mouth of the chromatography sampling bottle 120 faces upwards;
(2) placing a filter membrane 240 between the first well plate 210 and the second well plate 220;
(3) aspirating the sample to be filtered through the liquid inlet 251 of the volume tube 250 on the first well plate 210;
(4) the sample to be filtered is pressurized using a gas pressurizing pump 255 via a gas inlet 252 of the volumetric tube 250 on the first well plate 210 so that the sample to be filtered enters the chromatography sample bottle 120 through the filter membrane 240.
The method is suitable for the treatment of the chromatographic sample injection bottle of the automatic sample injection system, so that the filtering method can conveniently and quickly realize the filtering of the sample to be filtered, and the filtering system can realize that batch samples are simultaneously filtered through the filter membrane and enter different chromatographic sample injection bottles so as to be conveniently butted with the subsequent high-efficiency liquid-phase automatic sample injection process in time.
It should be noted that the features and advantages described above with respect to the filtering system 1000 are also applicable to the filtering method of the present invention, and will not be described herein again.
In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples and features of different embodiments or examples described in this specification can be combined and combined by one skilled in the art without contradiction.
Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art within the scope of the present invention.
Claims (9)
1. A filtration system, comprising:
a sample tray having a plurality of placement holes adapted to place chromatography vials;
the device comprises a filtration module, a liquid inlet and a gas inlet, wherein the filtration module comprises a first pore plate and a second pore plate which are attached to each other, at least one filtration membrane is fixed between the first pore plate and the second pore plate, a volume tube is arranged on the first pore plate at a position corresponding to a chromatogram sampling bottle, and the volume tube is provided with the liquid inlet and the gas inlet; the second pore plate is provided with a filter opening at the position corresponding to the chromatographic sample feeding bottle, the filter opening is communicated with the chromatographic sample feeding bottle, and the positions of the volume tube, the filter membrane, the filter opening and the chromatographic sample feeding bottle are vertically corresponding;
the plurality of chromatographic sample feeding bottles are arranged in the plurality of placing holes in a one-to-one correspondence manner;
the filter port is communicated with the chromatogram sample feeding bottle through a filter pipe;
the filter membrane is an organic filter membrane or a water system filter membrane;
the first orifice plate and the second orifice plate are respectively provided with a first partition assembly and a second partition assembly, and the first partition assembly and the second partition assembly are matched to connect the first orifice plate and the second orifice plate;
each of the first partition assembly and the second partition assembly includes a plurality of first direction partitions and a plurality of second direction partitions, and a plurality of cells are defined between the first direction partitions and the second direction partitions.
2. The filtration system of claim 1, wherein the lower end of the filter tube is inserted into the chromatography sample vial and the lower end has an inclined surface.
3. The filtration system of claim 1, wherein the plurality of first direction partitions are parallel to each other, the plurality of second direction partitions are parallel to each other, and an angle a between the first direction partitions and the extending direction of the second direction partitions satisfies 0 < a ≦ 90 degrees.
4. The filtration system of claim 3, wherein the first direction spacer and the second direction spacer extend in directions perpendicular to each other, and the first and second orifice plates are divided into a plurality of square units by the plurality of first direction spacers and the plurality of second direction spacers, respectively;
the side length of the square unit is larger than the diameter of the filter membrane.
5. The filtration system of claim 1, wherein the first partition assembly comprises a first rib protruding toward the second orifice plate and/or a first groove recessed away from the second orifice plate;
the second partition assembly comprises a second groove matched with the first convex strip and/or a second convex strip matched with the first groove.
6. A filtration system according to claim 1, wherein the gas inlet is connected to a gas booster pump.
7. The filtration system of claim 1, wherein the filter membrane is a 0.45 micron microporous filter membrane.
8. The filtration system of claim 1, wherein the filtration module is removably coupled to the chromatography sample vial.
9. A method of filtering a sample using the filtration system of any one of claims 1-8, comprising the steps of:
(1) placing a chromatographic sample introduction bottle in the sample tray, wherein the bottle mouth of the chromatographic sample introduction bottle faces upwards;
(2) placing a filter membrane between the first and second well plates;
(3) aspirating a sample to be filtered through a liquid inlet of a volume tube on a first well plate;
(4) and pressing the sample to be filtered by using a gas pressurizing pump through a gas inlet of the volume tube on the first pore plate so that the sample to be filtered enters the chromatographic sampling bottle through the filter membrane.
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| CN110789818B (en) * | 2019-11-29 | 2020-12-29 | 云南省烟草质量监督检测站 | Chromatographic sample injection bottle transfer device and transfer method thereof |
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| GB0121189D0 (en) * | 2001-08-31 | 2001-10-24 | Diagnoswiss Sa | Apparatus and method for separating an analyte |
| WO2004034026A2 (en) * | 2002-10-10 | 2004-04-22 | Irm, Llc | Capacity altering device, holder and methods of sample processing |
| CN103663623B (en) * | 2012-08-31 | 2016-04-13 | 中国科学院地理科学与资源研究所 | A kind of Halite water divides filtration unit and method thereof and application |
| CN203507640U (en) * | 2013-08-12 | 2014-04-02 | 浙江硕华医用塑料有限公司 | Needle head filter and lower cover thereof |
| CN204065037U (en) * | 2014-02-28 | 2014-12-31 | 中国农业科学院农产品加工研究所 | A kind of full-automatic matrix dispersive solid-phase extraction pre-treatment all-in-one |
| CN103852366B (en) * | 2014-03-13 | 2016-04-27 | 浙江省海洋水产研究所 | Aquatic products detect the batch filtration unit used |
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