WO2003018824A3 - Verfahren zur enzymatischen reduktion von substraten mit molekularem wasserstoff - Google Patents

Verfahren zur enzymatischen reduktion von substraten mit molekularem wasserstoff Download PDF

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Publication number
WO2003018824A3
WO2003018824A3 PCT/DE2002/002775 DE0202775W WO03018824A3 WO 2003018824 A3 WO2003018824 A3 WO 2003018824A3 DE 0202775 W DE0202775 W DE 0202775W WO 03018824 A3 WO03018824 A3 WO 03018824A3
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WO
WIPO (PCT)
Prior art keywords
molecular hydrogen
nadph
substrates
enzymatic reduction
method used
Prior art date
Application number
PCT/DE2002/002775
Other languages
English (en)
French (fr)
Other versions
WO2003018824A2 (de
Inventor
Andreas Liese
Rita Mertens
Lasse Greiner
Den Ban Eyke Van
Huub Haaker
Original Assignee
Forschungszentrum Juelich Gmbh
Andreas Liese
Rita Mertens
Lasse Greiner
Den Ban Eyke Van
Huub Haaker
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Forschungszentrum Juelich Gmbh, Andreas Liese, Rita Mertens, Lasse Greiner, Den Ban Eyke Van, Huub Haaker filed Critical Forschungszentrum Juelich Gmbh
Priority to EP02767065A priority Critical patent/EP1419262A2/de
Publication of WO2003018824A2 publication Critical patent/WO2003018824A2/de
Publication of WO2003018824A3 publication Critical patent/WO2003018824A3/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/36—Dinucleotides, e.g. nicotineamide-adenine dinucleotide phosphate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/14—Glutamic acid; Glutamine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/22—Preparation of oxygen-containing organic compounds containing a hydroxy group aromatic

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  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Die Erfindung betrifft ein Verfahren zur enzymatischen Reduktion von Substraten mit molekularem Wasserstoff. Erfindungsgemäss wird ein Mediator mittels einer Hydrogenase mit Wasserstoff umgesetzt. Im besonderen kann dieses Verfahren angewandt werden, um chemische Mediatoren oder auch Cofactoren von Enzymen zu reduzieren. Haupteinsatzpunkt dieses Verfahrens liegt in der Reduktion von NADP+ zu NADPH bzw. Einsatz des im Patent beschriebenen Enzyms zur Cofaktorregenerierung von NADPH mit molekularem Wasserstoff. Vorteil dieses Verfahrens, gegenüber allen etablierten Verfahren zur Regenerierung von NADPH, liegt in der Verwendung des sehr kostengünstigen molekularen Wasserstoffs zur Generierung bzw. Regenerierung des Cofaktors und damit nicht der Bildung eines noch weiter abzutrennenden Nebenproduktes. In diesem Fall wird Wasser als Nebenprodukt gebildet. Als Katalysator Können verschiedene Aufreinigungen und Präparationen des Enzyms Pyrococcus furiosus eingesetzt werden.
PCT/DE2002/002775 2001-08-21 2002-07-27 Verfahren zur enzymatischen reduktion von substraten mit molekularem wasserstoff WO2003018824A2 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP02767065A EP1419262A2 (de) 2001-08-21 2002-07-27 Verfahren zur enzymatischen reduktion von substraten mit molekularem wasserstoff

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE2001139958 DE10139958A1 (de) 2001-08-21 2001-08-21 Verfahren zur enzymatischen Reduktion von Substraten mit molekularen Wasserstoff
DE10139958.8 2001-08-21

Publications (2)

Publication Number Publication Date
WO2003018824A2 WO2003018824A2 (de) 2003-03-06
WO2003018824A3 true WO2003018824A3 (de) 2003-10-02

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2002/002775 WO2003018824A2 (de) 2001-08-21 2002-07-27 Verfahren zur enzymatischen reduktion von substraten mit molekularem wasserstoff

Country Status (3)

Country Link
EP (1) EP1419262A2 (de)
DE (1) DE10139958A1 (de)
WO (1) WO2003018824A2 (de)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102004007029A1 (de) * 2004-02-12 2005-09-08 Consortium für elektrochemische Industrie GmbH Verfahren zur enantioselektiven Reduktion von Ketoverbindungen durch Enzyme
WO2007084738A2 (en) * 2006-01-20 2007-07-26 Chemic Laboratories, Inc. Synthesis of (6s)-5-methyl-5,6,7,8-tetrahydrofolic acid
JP2013511973A (ja) * 2009-11-30 2013-04-11 ファルマツェル、ゲーエムベーハー 新規７β−ヒドロキシステロイドデヒドロゲナーゼおよびその使用

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
BRYANT F O ET AL: "CHARACTERIZATION OF HYDROGENASE FROM THE HYPERTHERMOPHILIC ARCHAEBACTERIUM PYROCOCCUS-FURIOSUS", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 264, no. 9, 1989, pages 5070 - 5079, XP001161213, ISSN: 0021-9258 *
GUNTHER H ET AL: "On a new artificial mediator accepting NADP(H) oxidoreductase from Clostridium thermoaceticum", JOURNAL OF BIOTECHNOLOGY, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 83, no. 3, 13 October 2000 (2000-10-13), pages 253 - 267, XP004225789, ISSN: 0168-1656 *
KIM CHULHWAN ET AL: "Stability and sulfur-reduction activity in non-aqueous phase liquids of the hydrogenase from the hyperthermophile Pyrococcus furiosus.", BIOTECHNOLOGY AND BIOENGINEERING, vol. 65, no. 1, 5 October 1999 (1999-10-05), pages 108 - 113, XP002246710, ISSN: 0006-3592 *
MA KESEN ET AL: "Characterization of hydrogenase II from the hyperthermophilic archaeon Pyrococcus furiosus and assessment of its role in sulfur reduction.", JOURNAL OF BACTERIOLOGY, vol. 182, no. 7, April 2000 (2000-04-01), pages 1864 - 1871, XP002246711, ISSN: 0021-9193 *
VERHAGEN M F J M ET AL: "The hyperthermophilic bacterium, Thermotoga maritima, contains an unusually complex iron-hydrogenase: amino acid sequence analyses versus biochemical characterization", BIOCHIMICA ET BIOPHYSICA ACTA. BIOENERGETICS, AMSTERDAM, NL, vol. 1412, no. 3, 4 August 1999 (1999-08-04), pages 212 - 229, XP004338894, ISSN: 0005-2728 *
VIGNAIS P M ET AL: "Continuous monitoring of the activation and activity of [NiFe]-hydrogenases by membrane-inlet mass spectrometry", INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, ELSEVIER SCIENCE PUBLISHERS B.V., BARKING, GB, vol. 27, no. 11-12, November 2002 (2002-11-01), pages 1441 - 1448, XP004381771, ISSN: 0360-3199 *
WONG C-H ET AL: "ENZYME CATALYZED ORGANIC SYNTHESIS NADPH REGENERATION USING DI HYDROGEN AND THE HYDROGENASE EC-1.2.1.2 FROM METHANOBACTERIUM-THERMOAUTOTROPHICUM", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 103, no. 20, 1981, pages 6227 - 6228, XP001161209, ISSN: 0002-7863 *
WOODWARD J ET AL: "Enzymatic production of biohydrogen.", NATURE. ENGLAND 29 JUN 2000, vol. 405, no. 6790, 29 June 2000 (2000-06-29), pages 1014 - 1015, XP002246709, ISSN: 0028-0836 *
WOODWARD JONATHAN ET AL: "In vitro hydrogen production by glucose dehydrogenase and hydrogenase.", NATURE BIOTECHNOLOGY, vol. 14, no. 7, 1996, pages 872 - 874, XP008019329, ISSN: 1087-0156 *

Also Published As

Publication number Publication date
EP1419262A2 (de) 2004-05-19
DE10139958A1 (de) 2003-03-20
WO2003018824A2 (de) 2003-03-06

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