WO2003014734A1 - Procede de detection d'une interaction entre une substance et une proteine, procede d'analyse de la proteine interagissant avec la substance et procede de formation d'un complexe contenant la substance et la proteine interagissant avec cette substance - Google Patents

Procede de detection d'une interaction entre une substance et une proteine, procede d'analyse de la proteine interagissant avec la substance et procede de formation d'un complexe contenant la substance et la proteine interagissant avec cette substance Download PDF

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Publication number
WO2003014734A1
WO2003014734A1 PCT/JP2002/008078 JP0208078W WO03014734A1 WO 2003014734 A1 WO2003014734 A1 WO 2003014734A1 JP 0208078 W JP0208078 W JP 0208078W WO 03014734 A1 WO03014734 A1 WO 03014734A1
Authority
WO
WIPO (PCT)
Prior art keywords
protein
substance
bait
prey
complex
Prior art date
Application number
PCT/JP2002/008078
Other languages
English (en)
French (fr)
Inventor
Hiroshi Yanagawa
Etsuko Miyamoto
Nobutaka Matsumura
Nobuhide Doi
Seiji Tateyama
Masamichi Ishizaka
Kenichi Horisawa
Original Assignee
Keio University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Keio University filed Critical Keio University
Priority to JP2003519415A priority Critical patent/JP3706942B2/ja
Publication of WO2003014734A1 publication Critical patent/WO2003014734A1/ja

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
PCT/JP2002/008078 2001-08-07 2002-08-07 Procede de detection d'une interaction entre une substance et une proteine, procede d'analyse de la proteine interagissant avec la substance et procede de formation d'un complexe contenant la substance et la proteine interagissant avec cette substance WO2003014734A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2003519415A JP3706942B2 (ja) 2001-08-07 2002-08-07 物質と蛋白質との間の相互作用の検出方法、物質と相互作用する蛋白質のスクリーニング方法、及び、物質とその物質と相互作用する蛋白質との複合体の形成方法

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP2001239175 2001-08-07
JP2001-239175 2001-08-07
JP2002-172129 2002-06-12
JP2002172129 2002-06-12

Publications (1)

Publication Number Publication Date
WO2003014734A1 true WO2003014734A1 (fr) 2003-02-20

Family

ID=26620098

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2002/008078 WO2003014734A1 (fr) 2001-08-07 2002-08-07 Procede de detection d'une interaction entre une substance et une proteine, procede d'analyse de la proteine interagissant avec la substance et procede de formation d'un complexe contenant la substance et la proteine interagissant avec cette substance

Country Status (2)

Country Link
JP (1) JP3706942B2 (ja)
WO (1) WO2003014734A1 (ja)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005024018A1 (ja) * 2003-09-08 2005-03-17 Zoegene Corporation 核酸構築物およびその製造方法
EP1736781A1 (en) * 2004-04-16 2006-12-27 Olympus Corporation Effective method of function analysis and screening of protein utilizing fluorescent generated by cell-free protein synthesizing system
US7842476B2 (en) 2002-09-06 2010-11-30 Isogenica Limited In vitro peptide expression library
JP2019037248A (ja) * 2012-07-03 2019-03-14 インテグレイテツド・デイー・エヌ・エイ・テクノロジーズ・インコーポレイテツド Ｔｍ増強ブロッキングオリゴヌクレオチド、ならびに標的濃縮の改善およびオフターゲット選択の低減のためのベイト
WO2023024066A1 (zh) * 2021-08-27 2023-03-02 中国科学院深圳先进技术研究院 临近标记复合物、临近标记方法、分子间互作分析方法

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5430804B1 (ja) 2012-03-30 2014-03-05 積水メディカル株式会社 粒子凝集測定用ラテックス粒子

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998016636A1 (fr) * 1996-10-17 1998-04-23 Mitsubishi Chemical Corporation Molecule permettant d'homologuer un genotype et un phenotype, et utilisation de celle-ci
JPH11322781A (ja) * 1998-05-15 1999-11-24 Mitsubishi Chemical Corp タンパク質のラベル化化合物およびその化合物を用いたタンパク質のラベル化方法
JP2000139468A (ja) * 1998-11-11 2000-05-23 Mitsubishi Chemicals Corp Ｃ末端がラベル化されたタンパク質の製造方法
JP2001027633A (ja) * 1999-05-07 2001-01-30 Inst Of Physical & Chemical Res 相互作用するタンパク質の検出方法
JP2001128690A (ja) * 1999-08-26 2001-05-15 Mitsubishi Chemicals Corp タンパク質−ｄｎａ連結分子及びその利用
JP2002176987A (ja) * 2000-12-14 2002-06-25 Keio Gijuku 遺伝子型と表現型の対応付け分子とその構成要素、および対応付け分子の製造方法と利用方法

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998016636A1 (fr) * 1996-10-17 1998-04-23 Mitsubishi Chemical Corporation Molecule permettant d'homologuer un genotype et un phenotype, et utilisation de celle-ci
JPH11322781A (ja) * 1998-05-15 1999-11-24 Mitsubishi Chemical Corp タンパク質のラベル化化合物およびその化合物を用いたタンパク質のラベル化方法
JP2000139468A (ja) * 1998-11-11 2000-05-23 Mitsubishi Chemicals Corp Ｃ末端がラベル化されたタンパク質の製造方法
JP2001027633A (ja) * 1999-05-07 2001-01-30 Inst Of Physical & Chemical Res 相互作用するタンパク質の検出方法
JP2001128690A (ja) * 1999-08-26 2001-05-15 Mitsubishi Chemicals Corp タンパク質−ｄｎａ連結分子及びその利用
JP2002176987A (ja) * 2000-12-14 2002-06-25 Keio Gijuku 遺伝子型と表現型の対応付け分子とその構成要素、および対応付け分子の製造方法と利用方法

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7842476B2 (en) 2002-09-06 2010-11-30 Isogenica Limited In vitro peptide expression library
US8679781B2 (en) 2002-09-06 2014-03-25 Isogenica Limited In vitro peptide expression library
WO2005024018A1 (ja) * 2003-09-08 2005-03-17 Zoegene Corporation 核酸構築物およびその製造方法
EP1736781A1 (en) * 2004-04-16 2006-12-27 Olympus Corporation Effective method of function analysis and screening of protein utilizing fluorescent generated by cell-free protein synthesizing system
EP1736781A4 (en) * 2004-04-16 2009-09-02 Olympus Corp EFFECTIVE METHOD FOR FUNCTIONAL ANALYSIS AND SCREENING OF PROTEIN USING A FLUORESCENT MADE WITH A CELL-FREE PROTEIN SYNTHESIS SYSTEM
JP2019037248A (ja) * 2012-07-03 2019-03-14 インテグレイテツド・デイー・エヌ・エイ・テクノロジーズ・インコーポレイテツド Ｔｍ増強ブロッキングオリゴヌクレオチド、ならびに標的濃縮の改善およびオフターゲット選択の低減のためのベイト
JP2021104032A (ja) * 2012-07-03 2021-07-26 インテグレイテツド・デイー・エヌ・エイ・テクノロジーズ・インコーポレイテツド Ｔｍ増強ブロッキングオリゴヌクレオチド、ならびに標的濃縮の改善およびオフターゲット選択の低減のためのベイト
JP7358413B2 (ja) 2012-07-03 2023-10-10 インテグレイテツド・デイー・エヌ・エイ・テクノロジーズ・インコーポレイテツド Ｔｍ増強ブロッキングオリゴヌクレオチド、ならびに標的濃縮の改善およびオフターゲット選択の低減のためのベイト
WO2023024066A1 (zh) * 2021-08-27 2023-03-02 中国科学院深圳先进技术研究院 临近标记复合物、临近标记方法、分子间互作分析方法

Also Published As

Publication number Publication date
JPWO2003014734A1 (ja) 2004-11-25
JP3706942B2 (ja) 2005-10-19

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